INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
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2023
·Vol. 73
·期 2
·005648
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IF 2.8
Rashidin Abdugheni,Dan-Hua Li,Yu-Jing Wang,Meng-Xuan Du,Nan Zhou,Chang Liu,Shuang-Jiang Liu
The human gastrointestinal tract is inhabited by various microorganisms, including thousands of bacterial taxa that have yet to be cultured and characterized. In this report, we describe the isolation, cultivation, genotypic and phenotypic characterization and taxonomy of five novel anaerobic bacterial strains that were recovered during the massive cultivation and isolation of gut microbes from human faecal samples. On the basis of the polyphasic taxonomic results, we propose two novel genera and five novel species. They areAcidaminococcus hominissp. nov. (type strain NSJ-142T=CGMCC 1.17903T=KCTC 25346T),Amedibacillus hominissp. nov. (type strain NSJ-176T=CGMCC 1.17933T=KCTC 25355T),Lientehia hominisgen. nov. sp. nov. (type strain NSJ-141T=CGMCC 1.17902T=KCTC 25345T),Merdimmobilis hominisgen. nov. sp. nov. (type strain NSJ-153T=CGMCC 1.17915T=KCTC 25350T) andParaeggerthella hominissp. nov. (type strain NSJ-152T=CGMCC 1.17914T=KCTC 25349T).
研究领域:
微生物学
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分类学
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胃肠道
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细菌学
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PubMed
高影响因子
Frontiers in Microbiology
·
2024
·
IF 4
Qiannan Li,Hanyu Pan,Peng Hao,Zhenhua Ma,Xiaojun Liang,Lianyu Yang,Yunhang Gao
The 17β-estradiol (E2)-degrading bacterium Rhodococcus sp.RCBS9 previously showed remarkable resistance to the combined stresses of low temperature and E2. In this study, physiological experiments and transcriptomic analysis were performed to investigate the mechanisms underlying the strain's low-temperature adaptation and briefly analyze how it maintains its ability to degrade E2 at low temperature. The results showed that the strain's signal transduction functions, adaptive changes in cell membrane and cell wall structure, gene repair functions, and synthesis of antioxidants and compatible solutes are key to its ability to adapt to low temperature. In addition, its stress proteins in response to low temperature were not typical cold shock proteins, but rather universal stress proteins (USPs) and heat shock proteins (HSPs), among others. The strain also upregulated biofilm production, transporter proteins for carbon source uptake, and proteins for fatty acid degradation to ensure energy generation. The strain's multiple stress responses work synergistically to resist low-temperature stress, ensuring its adaptability to low-temperature environments and ability to degrade E2. Finally, six genes related to survival at low temperature (identified in the transcriptome analysis) were expressed in E. coli BL21, and they were found to contribute to recombinant E. coli growth at low temperature.
研究领域:
微生物学
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环境科学
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分子生物学
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基因组学
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Xi Cao,Yayu Pan,Wei Wei,Tinglan Yuan,Sen Wang,Lingyun Xiang,Yongjun Yuan
Effects of various fermentation conditions on fatty acid composition of single cell oil (SCO) from Trichosporon sp. F1-2 were investigated. Ten carbon sources clustered into two groups using hierarchical clustering algorithm and principal component analysis. Xylose and soluble starch from each group were further studied. SCO using xylose had a high oleic acid content, while the linoleic acid and α-linolenic acid content increased significantly in SCO using soluble starch especially at the sn -2 position. 20 and 31 triacylglycerol (TAG) types were detected, respectively. The increased types were rich in linoleic acid and α-linolenic acid. The effect of C/N ratios was the weakest (distance threshold 3.05), followed by inoculation amounts (3.08), fermentation time (4.30) and nitrogen sources (5.64). The optimum nitrogen source, C/N ratio, inoculation amount and fermentation time for SCO accumulation were ammonium sulfate, 60, 6% and 120 h, respectively. Two different SCOs were produced using xylose and soluble starch under optimum conditions, which were demonstrated to be potential substrates for synthesis of 1,3-distearoyl-2-oleoyl-glycerol (a cocoa butter improver) and MLM-type TAG (the dietary TAG with low caloric value) respectively. Therefore, changing the carbon source can help broaden application fields of SCOs and increase feedstock sources of structured TAG synthesis.
研究领域:
生物技术
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食品科学
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微生物学
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CURRENT MICROBIOLOGY
·
2025
·Vol. 83
·期 2
·112
·
IF 2.6
Gao Peng,Lu Xie-Feng,Yao Ling,Xu Yuan-Jia,Zhang Shu-Yi,Liu Guo-Hong,Zhou Shun-Gui
Two novel aerobic, rod-shaped, motile bacterial strains, designated as strains sgz302251 T and sgz5001026 T , were both isolated from paddy soils. 16S rRNA gene sequences analysis revealed that strain sgz302251 T exhibited 98.3% similarity with its closest reference strain Paenibacillus vietnamensis N4 T , while strain sgz5001026 T had the highest similarity of 97.5% to Ectobacillus polymachus KACC 18242 T . The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strains sgz302251 T , sgz5001026 T and their closest reference strains were 86.0% and 21.2%, 84.9% and 22.9%, respectively. The genomic DNA G + C contents of sgz302251 T and sgz5001026 T were 63.4% and 68.8%, respectively. Strain sgz302251 T grew at 20–37 °C (optimum 30 °C), pH 5.0–9.0 (optimum 6.5–7.5) and tolerated 0–1.0% NaCl (optimum 0.5%, w/v), while strain sgz5001026 T grew at 4–45 °C (optimum 37 °C), pH 5.0–9.0 (optimum 6.0) and 0–0.5% NaCl (optimum 0%). The quinone in both strains was MK-7 and major polar lipids were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE) and phosphatidylglycerol (PG). The main fatty acids (> 5%) of strain sgz302251 T were anteiso-C 15:0 , anteiso-C 17:0 , and C 16:0 , whereas strain sgz5001026 T included anteiso-C 15:0 , iso-C 15:0 , iso-C 16:0 and iso-C 14:0 . Based on the above evidence, the proposed names of the two novel species are Paenibacillus praedii sp. nov. (type strain sgz302251 T = MCCC 1K09633 T = KCTC 43808 T ), Ectobacillus soli sp. nov. (type strain sgz5001026 T = MCCC 1K09373 T = KCTC 25903 T ), respectively.
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JOURNAL OF APPLIED MICROBIOLOGY
·
2021
·Vol. 131
·期 4
·1890-1908
·
IF 3.772
Win T.T.,Bo B.,Malec P.,Fu P.
AimsThis study sought to utilize indigenous soil micro‐organisms to suppress wilt‐causing fungal pathogens of the banana.Methods and ResultsFungal pathogens were isolated from wilt‐affected rhizospheric soil, and potential antagonistic bacterial strains were isolated from healthy rhizospheric soil in the same area from which fungal pathogens were isolated. The antifungal activity of isolated micro‐organisms against fungal pathogens was studied both in vitro and in vivo against fungal pathogens. It was found that Fusarium oxysporum and Alternaria sp. were pathogenic, while Penicillium sp., Bacillus velezensis and Bacillus subtilis were antagonistic. Moreover, it was seen that B. velezensis, B. subtilis and Penicillium sp. inhibited the growth of the two fungal pathogens in both in vitro and in vivo experiments. Further investigation indicated that B. velezensis, B. subtilis and Penicillium sp. were able to produce enzymatic antifungal compounds (chitinase and β‐1,3‐glucanase). The spray application around rhizome revealed that a combination of Bacillus spp. and Penicillium sp. in greenhouse conditions gave the highest reduction in disease severity by up to 60% to both fungal pathogens among the treatments.ConclusionsBanana disease is seen to be induced not only by F. oxysporum but also by Alternaria sp. The isolated indigenous micro‐organisms can effectively control both the pathogens. The combination of isolated antagonistic micro‐organisms has thus demonstrated substantial potential for suppressing banana disease.Significance and Impact of the StudyAn antagonistic consortium isolated in this study has demonstrated remarkable potential for controlling fungal diseases caused by Fusarium sp. and Alternaria sp. Therefore, the use of indigenous microflora to improve disease suppression of banana plants against soil‐borne pathogens is a preferable approach.
研究领域:
微生物学
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植物病理学
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农学
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AMB Express
·
2018
·Vol. 8
·期 1
·1-13
·
IF 1.719
Liang Jibei,Cheng Tao,Huang Yi,Liu Jianhua
Enhanced bioremediation is a favorable approach for petroleum pollutant cleanup, which depends on the growth of oil-eating microorganisms. In this study, we show that, by using the modified T-RFLP (mT-RFLP) methodology, one of the four major microbial populations derived from oil sludge has failed to propagate in MS medium supplemented with 2% yeast extract (YE). rDNA sequence-based analysis indicated that the four populations were Donghicola sp. CT5, Bacillus sp. CT6, Alcaligenes sp. CT10, and Pseudomonas sp. ZS1. Four purified strains grow well individually in MS medium supplemented with 2% YE, suggesting that ZS1 growth is antagonized by other strains. Co-growth analysis using mT-RFLP methodology and plate inhibitory assay indicated that ZS1 exhibited antagonistic effect against CT5 and CT6. On the other hand, co-growth analysis and plate inhibition assay showed that CT10 antagonized against ZS1. To investigate the potential compounds responsible for the antagonism, supernatant of CT10 culture was subjected to GC–MS analysis. Analysis indicated that CT10 produced a number of antimicrobial compounds including cyclodipeptide c-(L-Pro-L-Phe), which was known to inhibit the growth of Pseudomonas sp. Growth test using the purified c-(L-Pro-L-Phe) from CT10 confirmed its inhibitory activity. We further showed that, using both gravimetric and GC analysis, CT10 antagonism against the oil-eating ZS1 led to the diminishing of crude oil degradation. Together, our results indicate that bioremediation can be affected by environmental antagonists.
研究领域:
微生物学
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环境科学
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BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
·
2025
·Vol. 114
·期 2
·1-9
·
IF 2.2
Lin Shutao,Shi Chunhai,Wang Haimei,Ma Xiaoli,Li Jian,Chen Siqin,Guo Niuniu,Zhang Zhi
Ciprofloxacin (CIP) and oxytetracycline (OTC) are commonly detected antibiotic species in breeding wastewater, and microalgae-based antibiotic treatment technology is an environmentally friendly and cost-effective method for its removal. This study evaluated the effects of CIP and OTC on Scenedesmus sp . in the breeding wastewater tailwater and the removal mechanisms of antibiotics. The results showed that Scenedesmus sp could increase antibiotic tolerance by enhancing antioxidant system activity. Compared to CIP, Scenedesmus sp showed better performance for OTC removal, the removal efficiencies were 100%, 96.87%, 95.75%, 90.18% and 83.91% at 0.1, 0.5, 1, 5, and 10 mg L − 1 OTC, respectively. The removal routes indicated that CIP was mainly removed by biodegradation (38.88%) and photolysis (14.30%) whereas OTC was mainly removed by hydrolysis (43.47%) and biodegradation (33.45%). Product toxicity predictions showed that most of the degradation products of CIP and OTC were less toxic than their parent compounds, confirming the feasibility of microalgae biotreatment for antibiotic removal.
研究领域:
环境科学
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微生物学
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高影响因子
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
·
2025
·Vol. 75
·期 10
·006944
·
IF 2
Xie-Feng Lu,Peng Gao,Shu-Yi Zhang,Hui-Yuan Jiang,Guo-Hong Liu,Shun-Gui Zhou
Two novel aerobic, rod-shaped, non-motile and white-coloured bacterial strains, designated as sgz301288Tand sgz301302T, were isolated from paddy soil in Fujian Province. 16S rRNA gene phylogenetic analysis revealed that strain sgz301288Texhibited 97.2% similarity withAncylobacter moscoviensisKCTC 62336T, while strain sgz301302Thad the highest 16S rRNA gene sequence similarity toCiceribacter azotifigensKACC 19080T(97.4%) andEnsifer terangaeLMG 7834T(97.0%). Average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between sgz301288TandA. moscoviensisKCTC 62336Twere 79.5% and 22.9%, respectively. For strain sgz301302T, ANI and dDDH values were 76.7% and 21.5% withE. terangaeLMG 7834Tand 75.9% and 20.8% withC. azotifigensKACC 19080T. Phylogenomic analysis further supported strain sgz301288Tbelonging toAncylobacter, whereas sgz301302Tclustered withinEnsifer, distinct fromCiceribacter. The DNA G+C contents of sgz301288Tand sgz301302Twere 68.4% and 66.5 %, respectively. Strain sgz301288Tgrew at 20–30 °C (optimum 30 °C) and pH 6.5–9.5 (optimum 7.0) and tolerated up to 0–1.0% NaCl, while strain sgz301302Tgrew at 20–37 °C (optimum 30 °C), pH 5.0–9.5 (optimum 7.0) and 0–1.5 % NaCl. Both strains contained ubiquinone-10 as the predominant respiratory quinone. The main fatty acids were iso-C17 : 03-OH and summed feature 8 (C18 : 1ω7cand/or C18 : 1ω6c) for sgz301288Tand C16 : 0and summed feature 8 for sgz301302T. Based on the polyphasic evidence, the proposed names of the two novel species areAncylobacter terraesp. nov. (type strain sgz301288T=MCCC 1K09301T=KCTC 8858T) andEnsifer solisp. nov. (type strain sgz301302T=MCCC 1K09182T=KCTC 8857T), respectively.
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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
·
2025
·Vol. 75
·期 4
·006767
·
IF 2
Han-Yu Niu,Jie Zhang,Hao-Jie Huang,Xin-Wei Sun,Hao-Yu Chen,Xiao-Meng Wang,Ci Liu,Ming-Xia Bi,Shuang-Jiang Liu
Two Gram-stain-negative, strictly anaerobic, non-motile, non-spore-forming and rod-shaped bacterial strains, namely, P01024Tand P01025T, were isolated from piglet manure. The strains P01024Tand P01025Tfermented glucose to acetate and butyrate. The major cellular fatty acids (>10.0%) of strain P01024Twere C14 : 0, C16 : 0and summed feature 9 (iso-C17:1ω9c and/or 10-Methyl-C16:0) and of strain P01025Twere C14 : 0, C16 : 0and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c). Analysis of 16S rRNA gene sequences indicated that strains P01024Tand P01025Tbelonged to the familyOscillospiraceae. The strain P01024Tshowed high identities of 16S rRNA genes to type speciesFlavonifractor plautiiATCC 29863T(96.64%). The highest percentages of conserved protein (POCP) value between strain P01024TandFlavonifractor plautiiATCC 29863Twas 59.84%. The averagentidentity (ANI) and digital DNA–DNA hybridization (dDDH) values between strain P01024TandFlavonifractor plautiiATCC 29863Twere 79.51% and 23.80%, respectively, supporting that strain P01024Trepresented a novel species of the genusFlavonifractor. Strain P01025Tshowed high identities of 16S rRNA genes to the type speciesFlintibacter butyricusBLS21T(95.87%). The highest POCP and AAI (averageaaidentity) values of strains P01025TtoFlintibacter hominisNew-19Twere 53.02% and 73.11%, respectively. The ANI and dDDH values between strains P01025TandFlintibacter hominisNew-19Twere 75.44% and 23.40%, respectively, supporting that strain P01025Trepresented a novel species in the genusFlintibacter. The calculated G+C molar contents for strains P01024Tand P01025Twere 58.43 and 56.44 mol%, respectively. Together with phenotypic features, we concluded that strains P01024Tand P01025Trepresented novel species in the generaFlavonifractorandFlintibacterof the familyOscillospiraceae, respectively, for which the namesFlavonifractor porcisp. nov. (type strain P01024T=CGMCC 1.18055T=KCTC 25793T) andFlintibacter porcisp. nov. (type strain P01025T=CGMCC 1.18060T=KCTC 25794T) are proposed.
研究领域:
微生物学
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分子生物学
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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
·
2026
·Vol. 76
·期 4
·007124
·
IF 2.3
Guo-Hong Liu,Ling Yao,Hui-Yuan Jiang,Yuan-Jia Xu,Peng Gao,Xie-Feng Lu,Christopher Rensing,Shun-Gui Zhou
Biological nitrogen fixation is central to sustainable rice cultivation, yet diazotrophicPaenibacillusspecies from red paddy soils remain poorly characterized. Here, three novelPaenibacillusstrains, sgz500958T, sgz500992Tand sgz5001063T, were isolated from red paddy soils in China. Phylogenetic and phylogenomic analyses based on 16S rRNA and whole-genome data revealed that the three strains formed distinct lineages withinPaenibacillus, with average nucleotide identity values below 95% relative to their closest reference strains. All strains possessed a completenifgene cluster and exhibited nitrogenase activity, and strain sgz5001063Tmaintained high activity at low concentrations (0–3 mM), was inhibited at intermediate concentrations (4–30 mM) and recovered at very high ammonium concentrations (30–300 mM). Utilizing complementary plant growth-promoting traits and co-culture compatibility, two nitrogen-fixing synthetic communities (NFSCs) were assembled. In rice assays, NFSC2 markedly enhanced germination (100%), shoot height (+72.5%) and root growth relative to sgz5001063Talone and NFSC1. The isolates are proposed asPaenibacillus oryziterraesp. nov. (sgz500958T=MCCC 1K09377T=KCTC 43740T),Paenibacillus paludissp. nov. (sgz500992T=MCCC 1K09376T=KCTC 43729T) andPaenibacillus diazotrophicussp. nov. (sgz5001063T=MCCC 1K09391T=KCTC 43741T).
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BMC MICROBIOLOGY
·
2024
·Vol. 24
·期 1
·1-12
·
IF 4
Yang Wei-Kang,Gong Zhu,Wang Bao-Teng,Hu Shuang,Zhuo Ye,Jin Chun-Zhi,Jin Long,Lee Hyung-Gwan,Jin Feng-Jie
With the development of industry and modern manufacturing, nondegradable low-density polyethylene (LDPE) has been widely used, posing a rising environmental hazard to natural ecosystems and public health. In this study, we isolated a series of LDPE-degrading fungi from landfill sites and carried out LDPE degradation experiments by combining highly efficient degrading fungi in pairs. The results showed that the mixed microorganisms composed of Alternaria sp. CPEF-1 and Trametes sp. PE2F-4 (H-3 group) had a greater degradation effect on heat-treated LDPE (T-LDPE). After 30 days of inoculation with combination strain H-3, the weight loss rate of the T-LDPE film was approximately 154% higher than that of the untreated LDPE (U-LDPE) film, and the weight loss rate reached 0.66 ± 0.06%. Environmental scanning electron microscopy (ESEM) and Fourier transform infrared spectroscopy (FTIR) were used to further investigate the biodegradation impacts of T-LDPE, including the changes on the surface and depolymerization of the LDPE films during the fungal degradation process. Our findings revealed that the combined fungal treatment is more effective at degrading T-LDPE than the single strain treatment, and it is expected that properly altering the composition of the microbial community can help lessen the detrimental impact of plastics on the environment.
CAS / MDL:
研究领域:
环境科学
·
微生物学
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高影响因子
ENVIRONMENTAL POLLUTION
·
2023
·
IF 8.9
Edidiong Okokon Atakpa,Hanghai Zhou,Lijia Jiang,Dongdong Zhang,Yanhong Li,Wenjie Zhang,Chunfang Zhang
The widespread exploration and exploitation of crude oil has increased the prevalence of petroleum hydrocarbon pollution in the marine and coastal environment. Bioremediation of petroleum hydrocarbons using cell immobilization techniques is gaining increasing attention. In this study, the crude oil degradation performance of bacterial and fungal co-culture was optimized by entrapping both cells in sodium-alginate and polyvinyl alcohol composite beads. Results indicate that fungal cells remained active after entrapment and throughout the experiment, while bacterial cells were non-viable at the end of the experimental period in treatments with the bacterial-fungal ratio of 1:2. A remarkable decrease in surface tension from 72 mN/m to 36.51 mN/m was achieved in treatments with the bacterial-fungal ratio of 3:1. This resulted in a significant ( P < 0.05 ) total petroleum hydrocarbon (TPH) removal rate of 89.4%, and the highest degradation of n -alkanes fractions (from 2129.01 mg/L to 118.53 mg/L), compared to the other treatments. Whereas PAHs removal was highest in treatments with the most fungal abundance (from 980.96 μg/L to 177.3 μg/L). Furthermore, enzymes analysis test revealed that catalase had the most effect on microbial degradation of the target substrate, while protease had no significant impact on the degradation process. High expression of almA and PAH-RHDa genes was achieved in the co-culture treatments, which correlated significantly ( P < 0.05 ) with n -alkanes and PAHs removal, respectively. These results indicate that the application of immobilized bacterial and fungal cells in defined co-culture systems is an effective strategy for enhanced biodegradation of petroleum hydrocarbons in aqueous systems.
研究领域:
环境科学
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微生物学
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高影响因子
危险品化学品经营许可证（带存储）