CHEMICAL ENGINEERING JOURNAL
·
2017
·
IF 6.216
Yue Sun,Zimo Lou,Jianbing Yu,Xiaoxin Zhou,Dan Lv,Jiasheng Zhou,Shams Ali Baig,Xinhua Xu
Iron sulfide (FeS) nanoparticles were known for their excellent ability to scavenge aqueous Hg(II). However, the aggregation and oxidizability of FeS nanoparticles greatly restricted their practical application. This study developed an Al 2 O 3 -supported nanoscale FeS (FeS/Al 2 O 3 ) which effectively optimized the material performance. Characteristic data suggested that FeS/Al 2 O 3 (mass ratio of FeS to FeS/Al 2 O 3 is 30%) with a large specific surface area (142.7 m 2 g −1 ) could evenly distribute FeS, suppressing the aggregation of FeS successfully. The maximum enrichment capacity of Hg(II) on FeS/Al 2 O 3 achieved ∼313 mg/g at pH 6 and 30 °C. The pH studies indicated that alkaline environment strongly inhibited mercury uptake, while removal rate of 1 mg/L Hg(II) maintained a high level (>97.5%) over the pH range of 3–9. However, FeS/Al 2 O 3 loss was observed at low pH due to the acid corrosion. Additionally, the rates of Hg(II) reduction were almost unaffected over the range of 0.1–100 mM NaCl, but greatly inhibited by the presence of humid acid (HA). The synthetic FeS/Al 2 O 3 remained high mercury removal efficiency (over 95% in 1 mg/L Hg(II)) and low iron dissolution rate when preserved for 30 days, showing good long-term stability and remarkable application potential.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
Catalysts
·
2017
·Vol. 7
·期 11
·327
·
IF 3.082
Hao Liang,Shanshan Sun,Yan Zhou,Yanhui Liu
In this study, a one-step and facile immobilization of enzymes by self-assembly of zinc ions and adenine in aqueous solution with mild conditions was reported. Enzymes, such as glucose oxidase (GOx) and horseradish peroxidase (HRP), could be efficiently encapsulated in Zn/adenine coordination polymers (CPs) with high loading capacity over 90%. When the enzyme was immobilized by CPs, it displayed high catalytic efficiency, high selectivity and enhanced stability due to the protecting effect of the rigid framework. As a result, the relative activity of Zn/adenine nano-CP-immobilized GOx increased by 1.5-fold at pH 3 and 4-fold at 70 to 90 °C, compared to free GOx. The immobilized GOx had excellent reusability (more than 90% relative activity after being reused eight times). Furthermore, the use of this system as a glucose biosensor was also demonstrated by co-immobilization of two enzymes, detecting glucose down to 1.84 µM with excellent selectivity. The above work indicated that in-situ self-assembly of Zn/adenine CPs could be a simple and efficient method for biocatalyst immobilization.Keywords:adenine;enzyme;self-assembly;coordination polymers;immobilization
研究领域:
生物化学
·
纳米生物学
·
酶工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2018
·
IF 3.235
Xuejiao Liu,Wanwan Chen,Meiling Lian,Xu Chen,Yanluo Lu,Wensheng Yang
Metal-organic frameworks (MOFs) as a type of potential matrix for enzyme immobilization have attracted much interest. However, the sensitivity of most MOF-enzymatic electrochemical biosensors has not been satisfactory so far due to the poor conductivity and low apparent affinity of MOFs. In this work, new strategies have been presented: (i) using MOFs that themselves possess catalytic properties for substrates, so the synergistic catalysis by combining the MOFs and immobilized enzymes can be reinforced; (ii) introducing hydrophilic carbon nanomaterials and preparing MOF/carbon composites, not only the conductivity of the matrix but also the apparent affinity of immobilized enzyme for hydrophilic substrate can be remarkably enhanced. Horseradish peroxidase (HRP) and zeolite imidazolate framework-67(Co)/multi-walled carbon nanotube composite (ZIF-67(Co)/MWCNT) were adopted as the model enzyme and the immobilized matrix to illustrate and examine the proposed strategies. Compared with single component (ZIF-67(Co) or MWCNTs) based HRP electrochemical biosensors, the composite electrochemical biosensor displayed increased apparent substrate affinity (Michaelis constant K m reduced by ~75%) and 1.3-fold increased sensitivity in the detection of H 2 O 2 . The sensitivity (315 μA mM −1 cm −2 ) was also much larger than that of most previously reported MOF-HRP electrochemical biosensors. The proposed strategies are envisaged to be useful for other MOF-enzymes systems, which will be beneficial to the development of biocatalysis and biosensing.
研究领域:
生物化学
·
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
·
IF 5.162
Sizhu Ren,Ziyuan Wang,Muhammad Bilal,Yuxiao Feng,Yunhong Jiang,Shiru Jia,Jiandong Cui
Multienzymatic conversion of carbon dioxide (CO 2 ) into chemicals has been extensively studied. However, regeneration and reuse of co-factor are still the main problems for the efficient conversion of CO 2 . In this study, a nanoscale multienzyme reactor was constructed by encapsulating simultaneously carbonic anhydrase (CA), formate dehydrogenase (FateDH), co-factor (NADH), and glutamate dehydrogenases (GDH) into ZIF-8. In the multienzyme reactors, cationic polyelectrolyte (polyethyleneimine, PEI) was doped in the ZIF-8 by dissolving it in the precursors of ZIF-8. Co-factor (NADH) was anchored in ZIF-8 by ion exchange between PEI (positive charge) and co-factor (negative charge), and regenerated through GDH embedded in the ZIF-8, thus keeping high activity of FateDH. Activity recovery of FateDH in the multienzyme reactors reached 50%. Furthermore, the dissolution of CO 2 in the reaction solution was increased significantly by the combination of CA and ZIF-8. As a result, the nanoscale multienzyme reactor exhibited superior capacity for conversion of CO 2 to formate. Compared with free multienzyme system, formate yield was increased 4.6-fold by using the nanoscale multienzyme reactor. Furthermore, the nanoscale multienzyme reactor still retained 50% of its original productivity after 8 cycles, indicating excellent reusability.
研究领域:
生物技术
·
酶工程
·
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2018
·
IF 5.158
Lei Li,Feijun Wang,Ziqiang Shao,Jianxin Liu,Qiaoling Zhang,Weizhou Jiao
Magnetic fluorescent nanoparticles (MFNPs)-based protein immobilization systems were successfully synthesized via a layer-by-layer (LbL) assembly approach in an impinging stream-rotating packed bed, which adopted chitosan containing carbon dots (CDs)@Fe 3 O 4 nanoparticles (NPs) as a precursor, chitosan (CS) or carboxymethylcellulose (CMC) involving CDs as shells. To reveal the relationship between structure and efficiency of systems, the effect of self-assembly mode and layer number\was investigated to provide us insight into how to improve the design of MFNPs-based supports. Finally, an MFNPs-based protein immobilization system with excellent fluorescence and magnetic response, expanded specific surface area, and enhanced immobilization and release performance were obtained. Immobilization mechanism and kinetic studies were also conducted to reveal the rate-controlling step and the template affinity. Taken together, this study provides an effective strategy to synthesize bifunctional MFNPs and to adjust the performance, which aims for facilitating new possibilities for biomass-based nanomaterials used for protein immobilization.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2016
·
IF 3.138
Gang Fang,Honggao Chen,Yunpeng Zhang,Anqing Chen
Fe 3 O 4 magnetic nanometer particles (MNPS) with a diameter of 30–40 nm and coated by ammoniated silicon dioxides were developed by sol–gel method. The X-ray diffraction (XRD) pattern indicates that the Fe 3 O 4 is wrapped in the amorphous SiO 2 , and the Fourier-transform infrared spectroscopy (FT-IR) analysis confirmed the NH 2 group had been successfully introduced onto the surface of Fe 3 O 4 @SiO 2 nanoparticles. The main factors related to the immobilization process of pectinase onto the magnetic support were optimized by multi-factor orthogonal experiments, and the recommended combination is that 3% glutaraldehyde (coupling agent), pH = 8.0, 50 °C, and cross-linking time 8 h. Scanning electron microscope (SEM) and FT-IR analyses confirmed that the pectinase had been immobilized onto the surface of the amino modified nanoparticles. The storage stability and reusability of the pectinase were enhanced by the immobilization that 64.4% of the total starting activity was retrieved after 7 recycling batches and only about 21.1% of the total activity was lost after 30-day storage. For the application of the immobilized pectinase, the optimal pH and temperature were 8.5 and 55 °C, respectively.
研究领域:
生物化学
·
纳米生物学
·
酶工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROPOROUS AND MESOPOROUS MATERIALS
·
2016
·
IF 3.349
Jing Li,Lu-Shuang Li,Li Xu
In the present study, a hierarchically macro/mesoporous silica was fabricated, characterized and evaluated as a potential carrier for enzyme immobilization. The silica contained bicontinuous structure of skeletons and macropores, which were interwoven, thus forming a highly porous network. This unique structure rendered the silica as an efficient carrier for many applications, especially in which fast mass transfer was required. Herein, it was used as the carrier to immobilize catalase (CAT) for the catalytic purpose. The CAT loading amount can reach 285 mg/g (enzyme/carrier). As expected, the catalytic efficiency/activity of the immobilized catalase (IMCAT) was quite high (K m value of 176.54 mM), which was comparable with that of the free CAT (K m value of 169.78 mM). Additionally, the temperature and pH stability of IMCAT was almost the same as free CAT. However, it is noteworthy that, the IMCAT can be retrieved for repetitive usage. After 20 cycles, IMCAT still possessed relative activity more than 50%. The proposed hierarchically macro/mesoporous silica was demonstrated to be a promising carrier for enzyme immobilization and application, with high loading amount, excellent stability and desirable repeatability.
研究领域:
生物技术
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2013
·
IF 2.579
Zhongjie Wu,Wei Qi,Mengfan Wang,Yuefei Wang,Rongxin Su,Zhimin He
The ceramic powder with high metal content (MCP) was prepared as a new kind of immobilization carrier. Cu-MCP showed the best affinity to α-amylase and exhibited maximal activity. The effects of metal content, chelation time and amylase concentration on Cu-MCP-amylase activity were investigated. Under the optimal preparation conditions (the ratio of metal hydroxide precipitation to matrix (P/M): 0.23, chelation time: 8 h and amylase solution: 2.2 g/L), the activity of Cu-MCP-amylase could reach 31.5 U/g (54.5% of activity yield). Then, both Cu-MCP and Cu-MCP-amylase were characterized by XRD, SEM, BET-N 2 and FTIR. These characterizations confirmed that the Cu-MCP was a superior carrier with significant mechanical strength, considerable surface area, stable environment, homogeneous surface and low cost. Meanwhile, it also indicated that amylase had been efficiently immobilized on the external surface of Cu-MCP. Next, the properties of Cu-MCP-amylase were determined. It had optimal pH 7.0 and optimal temperature 70 °C with improved thermal stability. Finally, Cu-MCP-amylase showed significant catalytic capacity and stability in a column reactor, which could retain 80% of initial catalytic capacity after 40 recycle times.
研究领域:
生物化学
·
酶工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2019
·
IF 3.858
Jinheng Fu,Jinjing Li,Weiya Wang,Hongjing Wu,Peng Zhou,Yanping Li,Qinghua He,Zhui Tu
Affinity chromatography technologies play an important role in the purification of antibodies. To prepare affinity materials, prior isolation and purification of affinity ligands are required before coupling onto solid supports, which is quite expensive and laborious in large-scale applications. In this study, a one-step approach which circumvents the ligand purification procedures was developed to fabricate affinity gel for purifying immunoglobulin G (IgG). A self-labeling tag, haloalkane dehalogenase, was fused to the C-terminal of an anti-Fc variable domain of the heavy chain of the heavy-chain antibody (AFV) which was isolated in previous work. The AFV binds to various sources of IgG and is highly thermal stable. The fusion protein, namely HAFV, was expressed in Escherichia coli as a soluble protein. The binding affinity of HAFV to the Fc region of IgG was characterized and compared with the untagged anti-Fc nanobody. Next, the HAFV was immobilized directly from the crude cell lysate of isopropylthio-β-D-galactoside (IPTG) induced E. coli . The effects of NaCl concentrations and pH on the capacity of the HAFV resin were investigated. In addition, the one-step coupled HAFV resin was compared with the AFV resin and commercial resins (Protein A and Protein G) by evaluating the static capacity and stability. Though the Protein A (8.34 ± 0.37 mg/ml) and Protein G (9.19 ± 0.28 mg/ml) showed higher static capacity, the static capacity of HAFV resin (8.21 ± 0.30 mg/ml) was better than that of the untagged AFV gel (6.48 ± 0.56 mg/ml). The recovery results calculated for the reusability and stability show that there is no significant difference between the results obtained for the HAFV gel with those of the untagged AFV gel and commercial Protein A and G. After stored at 37 ℃ for 7 days and recycled 10 times, the static capacity of HAFV gel remains above 78%. Our strategy is site-specific, cost-effective, reproducible, and has the potential to dramatically cut down the costs of affinity materials for IgG purification.
研究领域:
生物化学
·
生物技术
·
分子生物学
·
蛋白质工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Research Express
·
2019
·Vol. 6
·期 11
·115526
·
IF 1.449
Kuibao Zhang,Baozhu Luo,Haibin Zhang
In this study, zirconolite-based ceramic powder was firstly fabricated from combustion reaction route. CeO2 was introduced as the surrogate of tetravalent actinides, which was directly immobilized by the single-phase zirconolite waste matrice. The results demonstrate that the crystalline incorporation of CeO2 is highly depended on the sintering temperature. 10 wt% CeO2 was directly immobilized into the zirconolite crystal structure under 1250 °C sintering, where Ce concurrently occupies the Ca and Zr sites. Higher CeO2 incorporation (15–25 wt%) leads to the formation of Ce-rich impurity phase and phase transformation from 2M-zirconolite to 3T-zirconolite. The Ce-bearing zirconolite waste forms exhibit the highest density and hardness after sintering at 1400 °C for 12 h. The aqueous durability was evaluated, where the 42 days LRCe values are 1.68 × 10–6 and 6.85 × 10–6 gm−2d−1 before and after the leachate treatment using nitric acid.
研究领域:
材料科学
·
核废料管理
·
陶瓷
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
BIOPROCESS AND BIOSYSTEMS ENGINEERING
·
2018
·Vol. 41
·期 7
·1051-1060
·
IF 2.139
Han Juan,Rong Junhui,Wang Yun,Liu Qian,Tang Xu,Li Cheng,Ni Liang
Abstract Magnetic double-shell hybrid microspheres (Fe 3 O 4 @SiO 2 @p(NIPAM- co -GMA)) have been developed as a promising supported substrate for the immobilization of cellulase. Since the surface of the magnetic microspheres not only contains an epoxy group from GMA (glycidyl methacrylate) that can covalently bind to the enzyme, but also has an intelligent temperature response property from NIPAM ( N -isopropylacrylamide), the cellulase can be covalently bonded to the magnetic microspheres and have a temperature-sensitive capability. The immobilized cellulase has the recovery ability of cellulase activity after a high-temperature inactivation. The average amount and activity of immobilized enzymes, respectively, was 233 mg g −1 , 57.4 U mg −1 under the optimized conditions. The experimental results show that the immobilized cellulase has a wider catalytic temperature range, better temperature and storage stability. The residual activity still remained about 65.6% of the initial activity after the sixth catalysis run, which indicated that the immobilized enzyme had high reproducibility. Graphical abstract
研究领域:
生物化学
·
生物技术
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2023
·
IF 5.8
Mianxing Luo,Liang Wang,Guo Chen,Jun Zhao
The orientation of the enzyme molecular on the interface of the carrier affects its activity. Therefore, it is very important to controllably induce the orientation of the enzyme on the surface to improve the performance of the immobilized enzyme . Magnetic nanoparticles were used to construct microenvironments with the different surface hydrophobicity and charge characteristics by controlled modification, and those particles with various microenvironments were further used to study their interaction with the lipase . The amount and activity of immobilized enzyme on different magnetic nanoparticles surfaces were studied by physical adsorption and covalent binding. Through the enzyme surface and particle surface characteristics analysis, the possible preferred orientation of enzyme and enzyme conformation on different surfaces were inferred, which well explained the effect of surface induction on enzyme loading and activity. The methods of surface microenvironment regulation and the strategy of controllable induction of enzyme orientation adopted in this study are enlightening for the rational design of immobilized enzyme methods.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）