TALANTA
·
2023
·
IF 6.1
Zichen Yang,Guoqing Chen,Chaoqun Ma,Jiao Gu,Chun Zhu,Lei Li,Hui Gao
Quinolone antibiotics have good antibacterial properties and are commonly used antibiotics in the dairy industry. Currently, the problem of excessive antibiotics in dairy products is very serious. As an ultra-sensitive detection technology, Surface-Enhanced Raman Scattering (SERS) was applied to the detection of quinolone antibiotics in this work. In order to classify and quantify three antibiotics (Ciprofloxacin, Norfloxacin, Levofloxacin) with highly similar molecular structures, a combination of magnetic COF-based SERS substrate and machine learning algorithms (PCA-k-NN, PCA-SVM, PCA-Decision Tree) was used. The classification accuracy of the spectral dataset could reach 100% and the results of LOD calculation were: CIP: 5.61 × 10 −9 M, LEV: 1.44 × 10 −8 M, NFX: 1.56 × 10 −8 M. This provides a new method for the detection of antibiotics in dairy products.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 12
·1-9
·
IF 5.833
Zhao Peng,Sun Jingyi,Zhao Shijun,Song Yanan,Gao Feng,Wang Hongbin,Ni Qingbin,Wang Ying,Sun Baoliang
A surface-enhanced Raman scattering (SERS)–based immunoassay with gold nanostars (GNSs) is utilized for determination of the subarachnoid hemorrhage (SAH) biomarker glial fibrillary acidic protein (GFAP) at very low concentration levels, which allows for early diagnosis and guides clinical decision-making to treat SAH-induced complications. The Raman reporter 5,5′-dithiobis-2-nitrobenzoic acid (DTNB) modified on GNSs was selected as the SERS tags. The SERS immunoassay was assembled by SERS tag and GFAP probe-immobilized ITO substrate. Therefore, the level of GFAP can be detected by monitoring the characteristic Raman peak intensity of GFAP-conjugated GNSs at 1332 cm −1 with a very low detection limit. Under optimized conditions, the assay can work in the GFAP concentration range from 1 pg⋅mL −1 to 1 μg⋅mL −1 , with a detection limit as low as 0.54 fg⋅mL −1 . The performance of the SERS immunoassay proven by the detection of GFAP is equivalent to that of the conventional enzyme-linked immunosorbent assay (ELISA). Graphical abstract Scheme 1. Schematic illustration of GNSs SERS immunoassay for ultrasensitive dynamic change detection of GFAP. (SAH: Subarachnoid hemorrhage, SCF: Cerebrospinal fluid; GNSs: gold nanostars; SERS: surface-enhanced Raman scattering; GFAP: glial fibrillary acidic protein)
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Peifang Chen,Caiyun Jiang,Zhouping Wang,Hong-zhen Lian,Xiaoyuan Ma
As a virulent metabolite, aflatoxin B 1 (AFB 1 ) presented in various cereal grain is tightly implicated in severe human diseases. In this study, 3D plasmonic nanohybirds of Raman molecule 4-mercaptobenzoic acid (4-MBA)-embedded and AFB 1 aptamer-modified bimetallic nanostars as SERS probes bound to magnetic nanoflowers were fabricated and demonstrated as a high-performance SERS-active aptasensor to quantitatively analyze AFB 1 . Bimetallic Au@Ag SERS plasmonic nanoprobes with enhanced properties were capable of enhancing discriminative Raman peaks of 4-MBA. Then, the integration of iron tetroxide nanoparticles (Fe 3 O 4 NPs) and molybdenum disulfide nanosheets (MoS 2 NShs) with huge specific surface area constituted stable 3D Fe 3 O 4 @MoS 2 plasmonic nanoflowers, facilitating the bind of numerous aptamer-based SERS probes via the non-covalent interaction between MoS 2 NShs and aptamer, which were ideal candidates for SERS-active substrates. Additionally, Fe 3 O 4 NPs as magnetic core endowed 3D nanocomposites with specific magnetic separation characteristic that caused the collected SERS hotspots to exhibit superior signal response, and further strengthening the sensitivity in a complex food matrix. Aptamer-target AFB 1 specific recognition triggered linearly diminished 4-MBA signal intensity (I 4-MBA ) on the substrate to achieve a low detection limit of 58.9 pg/mL. Furthermore, the sensor has the potential to be a promising monitoring tool for other trace contaminants.
✓ 已验证
产品关联
DOI
高影响因子
Nanomaterials
·
2020
·Vol. 10
·期 6
·1205
·
IF 4.324
Changbo Zhang,Jiying Xu,Yi Chen
Surface-enhanced Raman scattering (SERS) greatly increases the detection sensitivity of Raman scattering. However, its real applications are often degraded due to the unrepeatable preparation of SERS substrates. Herein presented is a very facile and cost-effective method to reproducibly produce a novel type of SERS substrate, a monolayer photonic crystal (PC). With a building block of laboratory-prepared monodisperse SiO2particles deposited with space-tunable silver nanobulges (SiO2@nAg), a PC substrate was first assembled at the air–water interface through needle tip flowing, then transferred onto a silicon slide by a pulling technique. The transferred monolayer PCs were characterized by SEM and AFM to have a hexagonal close-packed lattice. They could increase Raman scattering intensity by up to 2.2 × 107-fold, as tested with p-aminothiophenol. The relative standard deviations were all below 5% among different substrates or among different locations on the same substrate. The excellent reproducibility was ascribed to the highly ordered structure of PCs, while the very high sensitivity was attributed to the strong hotspot effect caused by the appropriately high density of nanobulges deposited on SiO2particles and by a closed lattice. The PC substrates were validated to be applicable to the SERS assay of trace thiol pesticides. Thiram pesticide is an example determined in apple juice samples at a concentration 102-fold lower than the food safety standard of China. This method is extendable to the analysis of other Raman-active thiol chemicals in different samples, and the substrate preparation approach can be modified for the fabrication of more PC substrates from other metallic nanobulge-deposited particles rather than silica only.Keywords:monolayer photonic crystal;silica particles;silver nanobulge deposition;SERS assay;pesticide determination
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Xiumei Zhuang,Yongjun Hu,Junjie Wang,Jieyu Hu,Qi Wang,Xingxing Yu
Tyrosinase (TYR) is as a well-known polyphenol oxidase and important biomarker of melanocytic lesions. Thus, developing powerful methods to determine TYR activity is of great value in the early diagnosis of skin disease. Direct surface-enhanced Raman scattering (SERS) detection of biomolecules is usually affected by non-specific interference and complicate structure of the analytes. It is a challenge to develop Raman-active molecules with specific recognition to analytes in complex media. Here, we report a novel colorimetric and surface-enhanced Raman scattering (SERS) dual-readout assay for the determination of TYR using commercially available and economical 4-mercaptophenyl boronic acid (4-MPBA) as a Raman-active and recognition molecule. 4-MPBA provides a unique interactive boronic acid group to the diol group of TYR substrate and exhibits good SERS signal. Also, the introduction of magnetic beads could promptly improve the anti-interference ability of dual-mode sensor. The TYR-incubated tyramine-modified magnetic beads could obviously change the concentration of 4-MPBA-AuNPs in the presence of O 2 and ascorbic acid , where the ultraviolet visible (UV–vis) absorption and SERS intensity were directly related to the concentration of TYR added. The dual-mode sensor had a rapid response to TYR within 1 min under optimized conditions and had high selectivity for TYR with a limit of detection at 0.001 U/mL. In addition, the dual-mode strategy showed promising prospects in the determination of TYR activity in serum samples and could be used to screen TYR inhibitors.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CELLULOSE
·
2021
·Vol. 28
·期 11
·7389-7404
·
IF 5.044
Xu Jiangtao,Shang Songmin,Gao Wei,Zeng Ping,Jiang Shouxiang
Although strategies of compositing noble materials and Zeolitic imidazolate frameworks (ZIFs) have been used to enhance the performance of ZIF-based surface-enhanced Raman scattering (SERS) substrates, the enhancement process still remains unclear and the sampling process with powder-form substrates is challenging for practical applications. In this study, a flexible SERS substrate with silver (Ag) nanoparticle decorated ZIF-67 as the active materials and cotton fabric as the supporting framework is developed with a facile method in two steps. The proposed flexible SERS substrate not only can reduce difficulties during the sampling process, but also expands future applications for sampling towards irregular target substances. Meanwhile, the enhancement mechanism has been investigated by using Methylene Blue to probe the molecular interactions. The constructed SERS substrate shows the highest enhancement factor of 6.25 × 10 6 and an excellent detection sensitivity with a limitation of detection (LoD) of 10 –14 M/L. Because of its excellent performance in SERS tracing, the proposed SERS substrate shows exceptional ability in detecting and identifying phenol-soluble modulins and is considered to be a label-free approach that requires no adding markers/tags or antibodies. The proposed substrate expands the potential applications of SERS technology for rapid detection of bacterial toxins during clinical diagnoses and treatment. Graphic abstract Ag decorated ZIF-67 particles are anchored onto cotton fabric to fabricate flexible SERS substrates. The proposed flexible SERS substrate achieves high SERS activity with high enhancement factor, low LoD, good reproducibility and stability. The flexible SERS substrate shows great potential in detection and identification of Phenol-soluble modulin.
✓ 已验证
产品关联
DOI
高影响因子
Nanomaterials
·
2021
·Vol. 11
·期 3
·672
·
IF 5.076
Rui Zhang,Yongchao Lai,Jinhua Zhan
The development of high-quality flexible surface-enhanced Raman spectroscopy (SERS) substrates is crucial for developing rapid SERS analysis in situ. Silver nanowire membranes as novel flexible substrates could benefit from the high collection efficiency of analytes by wrapping complex surfaces or wiping the surfaces of samples. However, their low SERS performance impedes further applications of silver nanowire membranes in analyte detection. Herein, we report an ultra-high-sensitivity silver nanowire membrane synthesized by a simple and time-saving cyclic voltammetry (CV) method. After CV treatment, a part of the silver nanowires on the silver nanowire membrane turned into small nanoparticles and nanorods. This nanostructure’s reconstitution increased the analytical enhancement factor of silver nanowire membranes by 14.4 times. Scanning and transmission electron microscopy, UV-vis spectroscopy, X-ray diffraction, and X-ray photoelectron spectroscopy were employed to investigate the transformation in the membrane nanostructure. The CV-treated substrates exhibited high surface-enhanced Raman activity and good temporal stability. The limits of detection (LODs) forp-aminothiophenol, crystal violet, tetramethylthiuram disulfide, sodium perchlorate, malachite green, fluoranthene, and potassium nitrate are 3.7 × 10−12M, 5.1 × 10−11M, 5.4 × 10−11M, 6.3 × 10−9M, 0.00693 ng, 0.0810 ng, and 0.0273 ng on this substrate, respectively. Additionally, the developed substrate is feasible for the detection of crystal violet in real samples. These results certify that CV-treated substrates possess broad application prospects in on-site SERS analysis.Keywords:surface-enhanced Raman spectroscopy;nanostructures reconstitution;silver nanowires;cyclic voltammetry;swabbing extraction
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Dongyue Lin,Yao He,Ronglu Dong,Wei Li,Fanli Meng,Yunfeng Zhang,Liangbao Yang
Succinylcholine chloride (SCC) is a common poison that threatens human life. At present, there is a lack of research on its on-site rapid detection methods. In this work, the use of gold nanorods as an enhanced substrate based on the high affinity between the quaternary ammonium salt structure can achieve rapid SERS detection of SCC in plasma. The long alkane chain structure of cetyltrimethylammonium bromide (CTAB) and the quaternary ammonium salt structure of SCC have a high molecular affinity, so that the target molecule can show a strong and obvious characteristic signal of SERS. Combined with a simple pretreatment method, acetonitrile is used as a protein precipitation agent to effectively remove matrix interference. The constructed SERS substrate can achieve the sensitive detection of 2 × 10 -8 M level of SCC in plasma samples and has high detection reproducibility. The entire pre-processing and testing process can be completed within 7 min, which can be used as an important technical basis for the preliminary identification of on-site SCC-related drug cases. The research results provide an effective solution for the establishment of SCC analysis strategies in complex matrices, and can provide new ideas for solving the problems of difficult identification of common poisons in the field and the lack of rapid detection methods on site.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2021
·Vol. 11
·期 33
·20326-20334
·
IF 3.361
Xiao-An Wang,Wei Shen,Binbin Zhou,Daoyang Yu,Xianghu Tang,Jinhuai Liu,Xingjiu Huang
Surface enhanced Raman spectroscopy (SERS) is a promising sensing technique that can provide unique chemical and structural fingerprint information, but gaining reliable SERS quantitative data with high sensitivity and stability still remains a challenge. Although using a molecule as an internal standard (IS) can improve the SERS quantitative capability, the reliability and SERS measuring conditions for signal fluctuations during calibration based on IS are yet to be explored when the embedded IS molecules and target objects are located in different environments. Herein, a 3D hotspot matrix SERS platform based on Au@4-MPy@AgNPs was constructed in water with the assistance of glycerol and the dynamic signal changes from the IS, i.e. 4-Mpy, and target molecules were monitored during the process of evaporation with high sensitivity and stability. In contrast to the traditional water-dispersed drying film system, the variation trends of IS and target molecules were consistent in the glycerol-assisted liquid film protection system. Therefore, it is reasonable to calibrate the signal fluctuation by utilizing the embedded IS based on the construction strategy of a glycerol-assisted 3D hotspot platform. This work demonstrates a rational, reliable and precise SERS quantitative technique for testing analyte concentrations in practical systems and has great application prospects in the field of analytical chemistry.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Hangqing Xie,Haogang Zhu,Lei Lei,Xiaolei Yang,Youjie Hua,Renguang Ye,Gongxun Bai,Shiqing Xu
Surface-enhanced Raman scattering (SERS) has attracted much attention with its powerful trace detection and analysis capabilities, especially biological and environmental molecules. However, building a protein SERS detection platform based on semiconductor devices is a huge challenge. Herein, through the synergy of NH 3 and nickel foam, a large-sized semiconductor tungsten oxide hydrate platform (WOHP) was synthesized. The crystal plane of a single WOHP particle is larger than the excitation spot. As a SERS substrate, WOHP can make full use of the excitation light without destroying the structure during the protein molecules detection process. Through the synergy of WOHP and Au NPs, the enhancement factor is 1.5 × 10 4 . Raman peaks of WOHP can be used as references for the detection of typical protein cytochrome C (Cyt C). As the Cyt C concentration decreases, the I Cyt C /I WOHP ratio decreases, and the signal can still be obtained when the concentration is as low as 5 × 10 −9 mol L −1 . More importantly, the method does not affect the catalytic activity of Cyt C and can be applied to the detection of Cyt C concentration in serum.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Optical Materials Express
·
2021
·Vol. 11
·期 7
·2001-2015
·
IF 3.442
Hui Ma,Linlin Xu,Yue Tian,Anxin Jiao,Mengya Zhang,Shuang Li,Shuang Li,Ming Chen,Ming Chen
Surface enhanced Raman spectroscopy (SERS) with both enhanced activity and pronounced thermal stability is very suitable for ultra-sensitive monitoring of thermally assisted chemical bonding/fragmentation reactions in important industrial catalysis. Herein, we report an appealing thermally stable SERS active sensor based on the construction of multiple-branched Au/Ag nanodendrites (NDs). The obtained Au/Ag NDs with tremendous elongated branches and enormous antennas exhibit a much higher SERS activity for dye detection under 785 nm near infrared (NIR) laser irradiation, as compared to as-prepared other reference samples. Meanwhile, the corresponding unique intermetallic synergy can effectively suppress the oxidation of chemically active Ag in a hot environment. It is important that more than 90% SERS activity at room temperature (∼25 ℃) can be well maintained at high temperature (∼170 ℃) within 30 min continuous tests. Thus, it holds great potential for the in-situ SERS monitoring of high-temperature catalytic reactions in the future.
✓ 已验证
产品关联
DOI
高影响因子
APPLIED SURFACE SCIENCE
·
2020
·
IF 6.182
Jie Yang,Gao Song,Ling Zhou,Xinyue Wang,Lijun You,Jumei Li
In this study, we successfully constructed Ag/TiO 2 core-shell nanoparticles through an easy wet chemistry method for SERS study. Compared to Ag cores, the Ag/TiO 2 core-shell nanoparticles presented higher SERS sensitivity, owing to a synergistic SERS enhancement arising from electromagnetic field effect of Ag cores and chemical enhancement of TiO 2 shells. Furthermore, uniform TiO 2 shells were able to effectively protect the internal Ag cores against destruction in external environment and thus storage stability of Ag/TiO 2 nanoparticles was prominently improved. The as prepared Ag/TiO 2 core-shell nanoparticles were applied to quantitatively sense tetramethylthiuram disulfide (TMTD) in solution using a portable Raman spectrometer. The results showed that the SERS intensity of 563 cm −1 assigned to S-S stretching mode had a good linear relationship with the concentration of TMTD with the correlation coefficient (R 2 ) of as high as 0.99 and the detection limit of as low as 1.15 × 10 −10 M. Consequently, the prepared Ag/TiO 2 nanoparticles present specially advantage of quantifying S-S groups by SERS, because the TiO 2 shell could act as a spacer between Ag cores and TMTD molecules and keep the S-S bonds intact.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）