Acta Biomaterialia
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2019
·
IF 6.638
Xiao Yang,Wen Liu,Yifen Shi,Guanghui Xi,Mingshan Wang,Bin Liang,Yakai Feng,Xiangkui Ren,Changcan Shi
It is challenging for traditional hemostatic sponges to meet the clinic demand for both uncontrolled and noncompressible hemorrhage. With the aim to develop a rapid shape recovery material with both active and passive hemostatic performance, a dual-functional hemostatic sponge (TRAP-Sp) with a macroporous structure and good mechanical properties for controlling massive and noncompressible hemorrhage was prepared by chemically immobilizing thrombin-receptor-agonist-peptide (TRAP) onto a starch/polyethylene glycol (PEG) sponge. The TRAP2-Sp1 showed the best hemostatic performance among all samples in both rat artery uncontrollable hemorrhage and liver defect noncompressible hemorrhage models. When analyzing the hemostatic mechanism of TRAP-Sp, the high water absorption capacity of the sponge contributed to absorbing plasma, concentrating blood cells, and enhancing blood coagulation . After absorbing water, the shape-fixed TRAP-Sp with sufficient mechanical strength and high resilience can rapidly expand and apply pressure to the wound. TRAP immobilized on the sponge could activate the adhered platelets in an active pathway. Additionally, evaluation of cytotoxicity, hemolysis, and histology further highlighted the adequate biocompatibility of TRAP-Sp. With excellent hemostatic performance and biosafety, this sponge could be a potential candidate as a topical hemostatic agent for uncontrolled and noncompressible hemorrhage. Statement of Significance There is a need for innovative hemostatic materials for both uncontrolled and noncompressible hemorrhage. This manuscript describes a rapid shape recovery hemostatic sponge with both active and passive hemostatic performances synthesized by foaming technique, cross-linking reaction, and chemical immobilization of thrombin-receptor-agonist-peptide (TRAP). On contact with blood, the shape-fixed sponge can not only rapidly recover its original shape and concentrate platelets and RBCs but also activate the adhered platelets efficiently. The dual-functional sponge has excellent hemostatic efficacy in rat femoral artery hemorrhage and can control noncompressible hemorrhage in penetrating liver wound. Thus, we believe that this sponge could be a potential candidate as a topical hemostatic agent for uncontrolled and noncompressible hemorrhage.
研究领域:
生物材料学
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医学工程
·
创伤修复
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PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2023
·
IF 7.7
Shiyu Bao,Likun Zhao,Yuanwen Liu,Lewei Yang,Peng Liu,Chaoqi Chen,Xuhui Mao
Polycyclic aromatic hydrocarbons (PAHs) are a group of contaminants with low bioavailability and biodegradability . As a result, removing PAHs from contaminated soils is often inefficient. In this study, soils spiked with fluoranthene and pyrene were inoculated with Aeromonas sp. BCP-3, a newly isolated strain from coke-plant sludge , and treated in slurry bioreactors . The removal of PAHs was significantly affected by aeration rate, slurry water-soil mass ratio, stirring rate, temperature, and the concentration of Tween-80. Such effects were attributed to changes in BCP-3 growth, PAHs solid-liquid mass transfer, and slurry particle size. Notably, under optimal conditions (i.e., aeration rate of 1.5 L/min, temperature of 30 °C, stirring rate of 200 rpm, water-soil mass ratio of 1.5:1, and Tween-80 concentration of 1.3 %), 96.3 % and 97.2 % of initially spiked fluoranthene and pyrene were removed within 12 days, respectively. A PAH-contaminated soil was collected from a local coking plant and treated under the same conditions, except for the Tween-80 concentration. For the real soil, the Tween-80 concentration was 4 % to ensure a significant proportion of PAHs released into the liquid phase. Twenty-one days after inoculation of BCP-3, the removal efficiencies of low molecular weight PAHs and high molecular weight PAHs were 82.2 % and 51.8 %, respectively. The removal efficiencies of PAHs were significantly higher than the slurry without BCP-3 or HgCl 2 -sterilized slurry. BCP-3 improved the bioavailability and biodegradability of PAHs, resulting in effective PAH removal in slurry bioreactors . Overall, successful bioaugmentation was achieved with the addition of Tween-80 and the optimization of operating conditions.
研究领域:
环境科学
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微生物学
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高影响因子
PLANTA MEDICA
·
2020
·
IF 2.687
Ying Gao,Jia Zhou,Hanli Ruan
Three new (alterchothecenes A – C, 1 –3) and 3 known (4 –6) trichothecenes, along with 9 known compounds (7 –15), were isolated from the culture of Alternaria sp. sb23, an endophytic fungus separated from the root of Schisandra sphenanthera Rehd. et Wils. Their structures were elucidated by spectroscopic analyses, and the absolute configurations of 1–3 were determined through comparison of the experimental electronic circular dichroism (ECD) spectra and optical rotations with similar analogues. In vitro cytotoxicity tests of compounds 1–6 against human HT-29 colon carcinoma and human MCF-7 breast cancer cell lines indicated that 4–6 exhibited significant cytotoxic effects, with IC50 values ranging from 0.89 to 9.38 µM. And the potential of compounds 1–6 as tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) sensitizers in HT-29 cells was evaluated. The results revealed that combination treatment of TRAIL with compounds 1–6 synergistically decreased cell viability compared with the sole treatment with those compounds.
研究领域:
药理学
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化学
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肿瘤学
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真菌学
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DOI
PubMed
高影响因子
CHEMISTRY & BIODIVERSITY
·
2025
·
IF 2.5
Jie Xu,Yanfang Yang,Qianqian Li,Ziyi Dong,Yaoyang Guo,Zhansheng Jiang
Ten chaetoglobosin alkaloids were isolated from the fermentation culture of a soil-derived fungus, Chaetomium sp., and their structures with absolute configurations were characterized by spectroscopic methods including high-resolution mass spectrometry (HRMS), nuclear magnetic resonance (NMR), X-ray diffraction analysis, and electronic circular dichroism, as well as comparison with literature data. Among them, three compounds ( 1 – 3 ) have not been described previously, and compound 8 is reported for the first time as a new natural product. A preliminary cytostatic screening revealed good inhibitory activity for 1 , 6 , and 8 against the A549 cell line (lung), and for 1 , 2 , 4 , 6 , and 8 against the MDA-MB231 cell line (breast). Further exploration demonstrated that compound 8 exerted in vitro antitumor activity by inducing significant apoptosis and S-phase cycle arrest, as well as blocking the migration and invasion of MDA-MB231 cells. Graphical
研究领域:
天然产物化学
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药理学
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微生物生物技术
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癌症研究
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生物有机化学
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PubMed
高影响因子
JOURNAL OF NATURAL PRODUCTS
·
2020
·
IF 3.782
Le Qin,Wenwen Yi,Xiao-Yuan Lian,Zhizhen Zhang
The new alkaloids marinacarbolines E–Q (1–10, 12–14), caerulomycin N (15), and actinoallonaphthyridine A (16), together with the known marinacarboline C (11) and cyanogramide (17), were isolated from the actinomycete Actinoalloteichus sp. ZZ1866. The structures of the isolated compounds were elucidated based on their HRESIMS data, extensive NMR spectroscopic analyses, Mosher’s method, ECD calculations, single-crystal X-ray diffraction analysis, and chemical degradation studies. Marinacarbolines E–L (1–8) share an indole-pyridone-imidazole tetracyclic skeleton, which is the first example of this kind of skeleton. Caerulomycin N (15) and cyanogramide (17) exhibited cytotoxic activity against both human glioma U251 and U87MG cells with IC50 values of 2.0–7.2 μM. Marinacarbolines E (1), G (3), I (5), and M (9) showed cytotoxic activity against U87MG cells with IC50 values of 2.3–8.9 μM.
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高影响因子
Advanced Science
·
2024
·Vol. 11
·期 29
·2401453
·
IF 14.3
Qian Xia,Shuoguo Yuan,Qiang Zhang,Can Huang,Jun Liu,Hongyun Jin
Li 1.3 Al 0.3 Ti 1.7 (PO 4 ) 3 (LATP) is one of the most attractive solid-state electrolytes (SSEs) for application in all-solid-state lithium batteries (ASSLBs) due to its advantages of high ionic conductivity, air stability and low cost. However, the poor interfacial contact and slow Li-ion migration have greatly limited its practical application. Herein, a composite ion-conducting layer is designed at the Li/LATP interface, which a MoS 2 film is constructed on LATP via chemical vapor deposition, followed by the introduction of a solid polymer (SP) liquid precursor to form a MoS 2 @SP protective layer. This protective layer not only achieves a lower Li-ion migration energy barrier, but also adsorbs more Li-ion, which is able to promote interfacial ion transport and improve interfacial contacts. Thanks to the improved migration and adsorption of Li-ion, the Li symmetric cell containing LATP-MoS 2 @SP exhibits a stable cycle of more than 1200 h at 0.1 mA cm −2 . More remarkably, the capacity retention of the full cell assembled with LiFePO 4 cathode is as high as 86.2% after 400 cycles at 1 C. This work provides a design strategy for significantly improving unstable interfaces of SSEs and realizing high-performance ASSLBs.
研究领域:
材料科学
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电化学
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DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2023
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IF 6
Huan Du,Yingyun Hu,Kaixiang Lu,Tao Li,Yang Tian,Yongjin Hu
Folate is an essential vitamin for the human body and must be obtained exogenously. It is recognized that some microorganisms are capable of producing folates during growth, revealing possibilities for innovations of natural folate biofortified foods. The aim of this study was to identify potential folate-producing strains to enhance folate content in fermented Moringa oleifera leaves powder (MOLP). Although most of the tested strains exhibited folate production, there were significant differences among them in terms of both quantity and distribution, whether intracellular or extracellular. 10 strains with potential for folate production were chosen for enhancing the folate content of MOLP. The two most promising strains, Lactiplantibacillus sp. LP1 and Saccharomyces cerevisiae Y-J-1, demonstrated high folate levels up to 1940 and 2115 μg/100 g dry matter, respectively. These levels were 1.6 and 1.8-fold the initial value in MOLP. Additionally, the fermentation of MOLP not only enhanced its nutritional quality but also preserved its significant bioactivity. This study provides the initial understanding of how selected microorganisms can naturally enrich folates in fermented MOLP, presenting an alternative solution to address the widespread issue of folate deficiency while promoting human health.
研究领域:
营养学
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微生物学
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食品科学
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生物技术
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DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2024
·
IF 5.7
Yu Zhang,Zeyuan Li,Shuxin Wei,Changsheng Xu,Minhua Chen,Jierong Sang,Yiru Han,Huang Yan,Zhoukun Li,Zhongli Cui,Xianfeng Ye
Researchers often consider microorganisms from Stenotrophomonas sp. to be beneficial for plants. In this study, the biocidal effects and action mechanisms of volatile organic compounds (VOCs) produced by Stenotrophomonas sp. NAU1697 were investigated. The mycelial growth and spore germination of Fusarium oxysporum f. sp. cucumerinum (FOC), which is a pathogen responsible for cucumber wilt disease, were significantly inhibited by VOCs emitted from NAU1697. Among the VOCs, 33 were identified, 11 of which were investigated for their antifungal properties. Among the tested compounds, 2-ethylhexanol exhibited the highest antifungal activity toward FOC, with a minimum inhibitory volume (MIV) of 3.0 μL/plate (equal to 35.7 mg/L). Damage to the hyphal cell wall and cell membrane integrity caused a decrease in the ergosterol content and a burst of reactive oxygen species (ROS) after 2-ethylhexanol treatment. DNA damage, which is indicative of apoptosis-like cell death, was monitored in 2-ethylhexanol-treated FOC cells by using micro-FTIR analysis. Furthermore, the activities of mitochondrial dehydrogenases and mitochondrial respiratory chain complex III in 2-ethylhexanol-treated FOC cells were significantly decreased. The transcription levels of genes associated with redox reactions and the cell wall integrity (CWI) pathway were significantly upregulated, thus indicating that stress was caused by 2-ethylhexanol. The findings of this research provide a new avenue for the sustainable management of soil-borne plant fungal diseases.
研究领域:
植物病理学
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微生物学
·
农业科学
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DOI
PubMed
高影响因子
LANGMUIR
·
2019
·
IF 3.683
Qingmei Peng,Xin Zhou,Zhi Wang,Qingyi Xie,Chunfeng Ma,Guangzhao Zhang,Xiangjun Gong
Surface stiffness plays a critical role in bacterial adhesion, but the mechanism is unclear since the bacterial motion before adhesion is overlooked. Herein, the three-dimensional (3D) motions of Escherichia coli and Pseudonomas sp. nov 776 onto poly(dimethylsiloxane) (PDMS) surfaces with varying stiffness before adhering were monitored by digital holographic microscopy (DHM). As Young’s modulus (E) of the PDMS surface decreases from 278.1 to 3.4 MPa, the adhered E. coli and Pseudonomas sp. decrease in number by 40.4 and 34.9%, respectively. Atomic force microscopy (AFM) measurements show that the adhesion force of bacteria to the surface declines with the decreased surface stiffness. In contrast, a nontumbling mutant of adhered E. coli (HCB1414 with the adaptive function being partially deficient) decreases much less (by 18.4%). On the other hand, the tumble frequency (Ft) of E. coli HCB1 and flick frequency (Ff) of Pseudomonas sp. increase as the surface stiffness decreases, and the motion bias (Bθ) of Pseudomonas sp. also increases. These facts clearly indicate that the bacteria have adapted responses to the surface stiffness. RNA sequencing (RNA-seq) reveals that the downregulated Cph2 and CsrA as well as the upregulated GcvA of swimming E. coli HCB1 in bulk near the softer surface promote the bacterial motility.
研究领域:
微生物学
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生物物理学
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DOI
高影响因子
ENVIRONMENTAL RESEARCH
·
2021
·
IF 6.498
Li Changmei,Wei Gengrui,Wu Haizhen,Wang Yuxiao,Zhu Shuang,Wei Chaohai
Polycyclic aromatic hydrocarbons (PAHs) and phenol are persistent pollutants that coexist in coking wastewater (CWW). Fluoranthene (Flu) is the predominant PAH species in the CWW treatment system. Our work emphasized on distinguishing the effects of phenol on Flu biodegradation by co-culture of Stenotrophomonas sp. N5 and Advenella sp. B9 and illustrated the molecular mechanisms. Results showed Flu biodegradation by co-culture was enhanced by phenol. According to the first-order degradation kinetic analysis of Flu, phenol significantly increased the biodegradation rate constant and shortened the half-life of Flu. Transcriptome analysis pointed out the up-regulation of DNA repair activity and 3717 significantly differentially expressed genes (DEGs), were triggered by 800 mg/L phenol. GO enrichment analysis suggested these DEGs are mainly concentrated in biochemical processes such as metal ion binding and alpha-amino acid biosynthesis, which are closely associated with Flu biodegradation, indicating that phenol promotes DNA repair activity and reduces Flu genotoxicity. qRT-PCR was performed to detect the gene expression of aromatic ring-opening dioxygenase. Combined with transcriptome analysis, the qRT-PCR results suggested phenol did not induce the expression of related PAHs-degrading enzymes. RNA extraction and microbial growth curves of COC and COC + Ph provided further evidence that phenol serves as co-substrate which increases biomass and the concentration of degrading enzymes, therefore promoting the Flu degradation.
研究领域:
环境科学
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微生物学
·
分子生物学
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DOI
高影响因子
PHYTOCHEMISTRY
·
2026
·
IF 3.4
Tong-De Zhao,Xue-Qiong Yang,Wei Chen,Hua Xu,Yan-Wei Yin,Yun-Li Zhao,Ya-Bin Yang,Zhong-Tao Ding
Candolleomyces sp. showed anti-PD in mice, and in vitro neuroprotective effect. • Candosin B had an unprecedented spiral 6/6/6/5-fused tetracyclic backbone. • Candosins A, C were undescribed 6/6/7/5-fused tetracyclic diterpenoid skeletons. • Candosins B-E, G-I, K and L showed obvious neuroprotective effects. Three fractions of Candolleomyces sp. cultured on Chinese yam agar solid medium were tested for the neuroprotective activity against Parkinson's disease (PD) induced by 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP) with model mice. Assessments including in vivo open field test (OFT), α-synuclein (α-syn) and tyrosine hydroxylase (TH) expression, pathological examination, and in vitro neuroprotective effects revealed that the dichloromethane fraction exhibited anti-PD effect. The work led to the isolation of 12 undescribed guanacastane diterpenoids candosins A–L ( 1 − 12 ). Candosin B ( 2 ) was an unprecedented diterpenoid with 6/6/6/5-fused tetracyclic backbone containing one [5.5] spiro ring. Candosins A and C ( 1 and 3 ) were undescribed diterpenoid skeletons with 6/6/7/5-fused tetracyclic backbone containing a pyran ring, and a furan ring. The structures were elucidated according to the NMR, HRESIMS, and ECD spectra. Compounds 2 – 5 , 7 – 9 , 11 , and 12 exhibited significant neuroprotective effects on MPP + induced PC-12 cell injury, with viability ranging from 68.63% to 87.97%. The docking results indicated that active diterpenoids produced by fungus Candolleomyces sp. might exert a protective effect on PD through α-syn mediated signaling pathway. The dichloromethane fraction of Candolleomyces sp., cultured on Chinese yam agar solid medium, exhibited anti-PD activity, leading to the isolation of 12 previously undescribed guanacastane diterpenoids, candosins A–L ( 1 – 12 ). Molecular docking suggested that α-synuclein (α-syn) may serve as a potential molecular target for the neuroprotective effects of compounds 2 – 5 , 7 – 9 , 11 , and 12 . Download: Download high-res image (433KB) Download: Download full-size image
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高影响因子
CURRENT MICROBIOLOGY
·
2026
·Vol. 83
·期 4
·207
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IF 2.8
Lu Xie-Feng,Gao Peng,Zhang Shu-Yi,Xu Yuan-Jia,Liu Guo-Hong,Chen Man,Zhou Shun-Gui
Two Gram-stain positive, motile, aerobic, yellowish rod-shaped bacterial strains sgz301303 and sgz301304 T were isolated from the paddy soils. The 16S rRNA gene phylogenetic analysis revealed that both strains belong to the genus Sphingobium , and exhibited the highest sequence similarity (98.3%) with reference strain Sphingobium amiense JCM 11777 T . The draft genome of sgz301304 T has a size of 4.3 Mbp, with DNA G + C content of 64.5%. Whole-genome relatedness analyses showed that the average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain sgz301304 T and S. amiense JCM 11777 T were 82.3% and 25.7%, respectively, both below the accepted species delineation thresholds. The main quinone was Q-10, and the main fatty acids included C 14:0 2-OH (11.3%), and Summed Features 8 (46.6%). Phylogenetic, genomic and phenotypic analyses suggest that strain sgz301304 T belongs to a novel species within the genus of Sphingobium . Therefore, it is recommended to be named as Sphingobium agrisoil sp. nov. The type strain is sgz301304 T (= MCCC 1K09180 T = JCM 37704 T ), respectively.
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高影响因子
危险品化学品经营许可证（带存储）