BIOSENSORS & BIOELECTRONICS
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2019
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IF 9.518
Weiran Mao,Junlin He,Zhiyong Tang,Chengli Zhang,Jun Chen,Jia Li,Chao Yu
A novel electrochemical immunosensor was proposed for the detection of monocyte chemoattractant protein-1 (MCP-1), a biomarker of cardiovascular disease. Due to thionine (Thi) possessing electroactive redox properties, a one-step approach was utilized to synthesize a reduced graphene oxide-tetraethylene-thionine-Au (rGO-TEPA-Thi-Au) nanocomposite at room temperature using the synergistic effect of Thi and rGO-TEPA towards HAuCl 4 . We obtained the excellent matrix material , which immobilized more primary antibody MCP-1-Ab 1 on rGO-TEPA on a modified glassy carbon electrode (GCE). To further enhance the sensitivity of the sensor, a novel signal generation and amplification strategy was developed for detection. RuPdPt trimetallic nanoalloy particles (RuPdPt TNPs), a novel nanomaterial , were synthesized by a one-pot method, displayed a uniform morphology as well as good electrochemical activity and bound with the secondary antibodies against MCP-1 via the Pt-NH 2 bond. Most importantly, RuPdPt TNPs have a significant ability to catalyze H 2 O 2 to produce an electron. The electrochemical signal was highly amplified because the electrochemical signal was primarily derived from the synergistic catalysis of H 2 O 2 by RuPdPt TNPs and recorded by chronoamperometry . Under the optimal conditions, this newly designed biosensor exhibited sensitive detection of MCP-1 in the range from 20 fg mL −1 to 1000 pg mL −1 , with a detection limit of 8.9 fg mL −1 (based on a S/N = 3). Additionally, the designed immunosensor showed acceptable selectivity , reproducibility and stability. This immunosensor is a promising strategy for analyzing clinical serum samples in the future.
研究领域:
生物化学
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纳米技术
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电化学
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生物传感器
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医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2018
·
IF 4.244
Jing Bao,Changjun Hou,Yanan Zhao,Xintong Geng,Mickey Samalo,Huisi Yang,Minghong Bian,Danqun Huo
In present study, a sensitive and effective electrochemical microRNA (miRNA) sensing platform is successfully developed by integrating gold nanoparticles/polypyrrole-reduced graphene oxide (Au/PPy-rGO), catalyzed hairpin assembly (CHA) and hybridization chain reaction (HCR) multiple signal amplification strategy. Firstly, Au/PPy–rGO was employed onto a bare GCE by electrodeposition that can greatly enhanced conductivity and effectively immobilize probes. Then, the thiolated capture probes (SH-CP) were self-assembled on the Au/PPy–rGO modified GCE via Au-S bond. The target miRNA triggered the dynamic assembly of the two hairpin substrates (H1 and H2), leading to the cyclicality of the target miRNA and the formation of H1–H2 complexes without the assistance of enzyme. Subsequently, the newly emerging DNA fragment of H2 triggered the HCR when a mixture solution (hairpins H3 and H4) and produced dsDNA polymers. Finally, a substantial amount of methylene blue (MB) as signal indicator was intercalated into the minor groove of the long dsDNA polymers to achieve detected electrochemical signal. The fabricated sensor is able to detect miRNA-16 (model target) with concentration range from 10 fM to 5 nM with a low detection limit (LOD) of 1.57 fM (S/N = 3). Current research suggests that the developed multiple signal amplification platform has a great potential for the applications in the field of biomedical research and clinical analysis.
研究领域:
生物医学工程
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分析化学
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纳米技术
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MATERIALS CHEMISTRY AND PHYSICS
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2024
·
IF 4.3
Hao Tian,Jie Ma,Bai Yang,Ran Li,Ronghai Yu
It is necessary to broaden absorption bandwidth, regulate applied frequency, reduce the weight and maintain strong absorption for magnetic microwave absorbing materials by constructing suitable material structures to satisfy the requirements for wide applications in various electromagnetic devices. In this work, well-structured amorphous-Co@Nd(BO 2 ) 3 /rGO nanocomposites have been fabricated using a selective chemical reduction in a mixed aqueous solution containing Co 2+ and Nd 3+ ions along with GO sheets reduced by NaBH 4 as the reductant, in which only Co 2+ and GO but no Nd 3+ ions were reduced. Very low coercivity of 0.4 Oe is achieved in amorphous-Co@Nd(BO 2 ) 3 /rGO nanocomposites due to amorphous Co@Nd(BO 2 ) 3 core-shell nanoparticles uniformly anchored on rGO sheets. These soft magnetic rGO-based nanocomposites exhibit high-frequency microwave absorption performance including a minimum reflection loss ( RL min ) value of −84.09 dB at 15.38 GHz for a 2.1 mm thickness at an applied frequency range (12.31–18 GHz) covering almost the entire Ku band. Moreover, a much wider absorption band of 7.24 GHz (10.67–17.89 GHz, RL ≤ −10 dB) with a 2.3 mm thickness along with a RL min value of −35.92 dB is also achieved. These rGO-based magnetic composites also show good structural stability , low density and easily controlled material compositions along with facile chemical fabrication suitable for mass production, which promises their latent use in high-performance microwave absorbing devices. Crystallization behavior of a new amorphous Nd(BO 2 ) 3 phase was also investigated.
研究领域:
材料科学
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电磁工程
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纳米技术
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
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2016
·Vol. 27
·期 9
·9278-9285
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IF 1.798
Liu Ziyu,Xu Guangliang,Zhang Ming,Xiong Kun,Meng Pingyuan
Cobalt ferrite/reduced graphene oxide (CoFe 2 O 4 /RGO) nanocomposites were initially prepared by the interaction of alkyne-functionalized CoFe 2 O 4 NPs and azide-functionalized graphene oxide (GO–N 3 ) via the Cu (I)-catalyzed click reaction. The morphology and structure of CoFe 2 O 4 /RGO were investigated by means of XRD, FTIR, TGA, TEM, SEM and the electromagnetic parameters were measured by vector network analyzer. For CoFe 2 O 4 /RGO composites with click reaction, the minimum reflection loss could reach −40 dB at 6.8 GHz with a matching thickness of 4.0 mm, and also it had a bandwidth below −10 dB ranging from 5.8 to 8.5 GHz. The CoFe 2 O 4 /RGO composites with click reaction exhibited better microwave absorbing performance than the physical mixture. Furthermore, the covalent ligation between CoFe 2 O 4 NPs and graphene oxide could make the composites have a promising future in electromagnetic wave absorption materials.
研究领域:
材料科学
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纳米技术
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电磁材料
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化学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
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2019
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IF 4.916
Xueliang Liu,Xinhuan Wang,Qiusen Han,Cui Qi,Chen Wang,Rong Yang
In this work, we reported a novel nanozyme synthesized by decorating highly dispersed ultrafine IrO 2 nanoparticles on reduced graphene oxide (rGO) nanosheets via a simple hydrothermal method . The as-prepared IrO 2 /rGO nanocomposites exhibited intrinsic peroxidase-like activity and could catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to produce blue product in the presence of H 2 O 2 . Catalytic kinetic of IrO 2 /rGO nanocomposites followed Michaelis-Menten behavior, exhibiting a higher affinity to TMB than horseradish peroxidase (HRP) enzyme. Catalytic mechanism studies suggested that the peroxidase-like activity of IrO 2 /rGO nanocomposites originated from their ability of electron transfer between substrate and H 2 O 2 . On the basis of high peroxidase-like activity of IrO 2 /rGO nanocomposites, a colorimetric strategy for rapid and sensitive detection of low weight biothiols was developed. The colorimetric detection assays for low weight biothiols showed high selectivity against other amino acids . Therefore, the IrO 2 /rGO nanozyme is expected for promising potential applications in the biosensor, diagnostics and environment.
研究领域:
纳米材料
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催化
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分析化学
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生物传感器
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生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2022
·
IF 4.598
Yuting Zhong,Zhirui Wu,Xiaoguang Liu,Ling Li
The combination of multi-metallic alloys and support materials is a promising approach for the design of electrocatalysts. In this study, Pd-M (M = Fe, Sb, Pb, Sn, Ag) bimetallic alloys embedded composites of 2D Ca-based metal–organic framework (Ca-MOF) nanosheets and reduced graphene oxide (rGO) were fabricated by a facile hydrothermal and impregnation reduction method, then applied as ethylene glycol oxidation reaction (EGOR) electrocatalysts. Remarkably, the forward peak current density of the best catalytic performer PdAg@rGO/Ca-MOF is as high as 258.30 mA cm −2 , which is 9.55 times greater than that of Pd/C. It also exhibits the biggest electrochemical active surface area of 3022.51 m 2 g −1 . After 3600 s chronoamperometry test, the retained current density of PdAg@rGO/Ca-MOF is about 87.94 % of the initial current density. These results imply that bimetallic Pd-M@rGO/Ca-MOF have superior electrocatalytic activity and anti-CO poisoning capacity compared to monometallic and Pd/C for EGOR, especially PdAg@rGO/Ca-MOF. The enhanced EGOR performance is due to the combined effect of Pd-M alloys and folded thin layer wrapped nanosheet rGO/Ca-MOF composites.
研究领域:
材料科学
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电化学
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催化
✓ 已验证
DOI
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 11
·1-17
·
IF 5.3
Liang Jintao,Liang Jianlu,Xiao Xinkai,Guo Fei,Zhan Yulian,Zhou Xueqing,Zhou Zhide,Li Guiyin
A sandwich-type electrochemical aptasensor for ultrasensitive detection of glypican-3 (GPC3) was constructed using GPC3 aptamer (GPC3 Apt ) labelled reduced graphene oxide-cerium oxide-gold nanoparticles (RGO-CeO 2 -Au NPs) as the signal probe and the same GPC3 Apt as the capture probe. The electrochemical redox properties of CeO 2 (Ce 3+ /Ce 4+ ) in the RGO-CeO 2 -Au NPs indicate the electrochemical signals. When the target GPC3 was present, an “aptamer-protein-aptamer” sandwich structure was formed on the sensing interface due to the specific binding between the protein and aptamers, resulting in an increased electrochemical redox signal detected by differential pulse voltammetry (DPV) technique. Under optimal conditions, the established aptasensor exhibited a logarithmic linear relationship between the current response and GPC3 concentration in the range 0.001–100.0 ng/mL, with a minimum detection limit of 0.74 pg/mL. Using the spike-recovery tests for measurement of the human serum samples, the recovery was from 99.26 to 114.01%, and the RSD range was 3.04 to 5.34%. Furthermore, the sandwich-type electrochemical aptasensor exhibited excellent performance characteristics such as good stability, high specificity, and high sensitivity, demonstrating effective detection of GPC3 in human serum samples and can be used as a clinical detection tool for GPC3. Graphical Abstract
研究领域:
电化学
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纳米技术
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生物传感器
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癌症诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
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2021
·
IF 19.503
Cheng-Xin Chen,Shan-Shan Yang,Jie Ding,Guang-Yuan Wang,Le Zhong,Shuang-Yang Zhao,Ya-Ni Zang,Jun-Qiu Jiang,Lan Ding,Yan Zhao,Lu-Ming Liu,Nan-Qi Ren
In this study, the effect of chloride ion (Cl − ) on the degradation of sulfadiazine (SDZ) was evaluated using reduced graphene oxide (rGO) modified with sodium anthraquinone-2-sulfonate (AQ2S) photocatalyst ( [email protected] ), which was prepared in a one-pot hydrothermal synthesis. AQ2S molecules are small organic photoactive components, that are intercalated and immobilized on the rGO surface through noncovalent π-π stacking. Under solar light, SDZ effectively underwent 99 % photodegradation within 150 min in a Cl − -rich solution (0.5 mol/L). The [email protected] system exhibited an excellent photocatalytic removal ability for some typical contaminants. SDZ degradation was driven by 3 AQ2S*. Superoxide radicals ( O 2 − ) and dichlorine radicals (Cl 2 − ) were found to be the dominant reactive species, whereas OH and 1 O 2 have minor roles. Density functional theory (DFT) calculations confirmed the interactions between AQ2S and rGO, and the electronic structural characteristics between Cl − and [email protected] The [email protected] composite has great potential for use in treating micropollutants under saline water conditions under solar irradiation.
研究领域:
环境科学
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纳米技术
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光化学
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材料科学
✓ 已验证
DOI
高影响因子
BIOELECTROCHEMISTRY
·
2021
·
IF 5.373
Jintao Liang,Xiaohang Shi,Huafu Feng,Min Chen,Wenzhan Li,Junxiang Lai,Weipeng Hu,Guiyin Li
In this paper, a novel silicon-based light-addressable potentiometric sensor (LAPS) has been designed for the detection of 1,5-anhydroglucitol (1,5-AG) in human serum. Reduced graphene oxide-chitosan-ferrocene (RGO-CS-Fc)/AuNPs nanohybrids and pyranose oxidase (PROD) enzyme is used to fabricate biological sensitive membrane unit by layer-by-layer assembly technology. When a bias voltage is provided to the LAPS system, the catalytic oxidation reaction between 1,5-AG and PROD to produce H 2 O 2 . The by-product H 2 O 2 can oxidize Fc(Fe 2+ ) ions in RGO-CS-Fc nanohybrids into Fc(Fe 3+ ) ions, which cause the potential of the sensitive membrane surface to change and the potential shift of I-V curve will generate a corresponding offset response. Under the optimal conditions, the potential shift of the LAPS is linearly related to the concentration of 1,5-AG at 10 µg·mL −1 –350 µg·mL −1 with the correlation coefficient of 0.97414. The sensitivity is 0.44273 mV/µg·mL −1 and the lowest detection limit is 10 µg·mL −1 . In addition, the biosensor showed good specificity, acceptable stability and satisfactory recovery rates (91.28%-107.66%), which would be a potential testing methods in actual clinical samples.
研究领域:
生物医学工程
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分析化学
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纳米技术
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生物传感器
✓ 已验证
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Yumei Chen,Jialin Zhang,RuiRui Liu,Ying Zhang,Jingming Zhou,Hongliang Liu,Yankai Liu,Ke Yan,Yanhua Qi,Enping Liu,Xifang Zhu,Aiping Wang
A label-free electrochemical immunosensor was developed to rapidly detect tilmicosin (TMC) residues in pork and milk. The immunosensor was constructed by immobilizing a high-affinity monoclonal antibody against TMC on an rGO-PEI-Ag nanocomposite-modified electrode. The rGO-PEI-Ag nanocomposites were prepared by mixing polyethyleneimine (PEI) modified reduced graphene oxide (rGO) with AgNO 3 solution. The prepared rGO-PEI-Ag nanocomposites showed good redox activity and conductivity, as characterized by ultraviolet-visible spectroscopy (UV–Vis), transmission electron microscopy (TEM), and X-ray diffraction (XRD). During the preparation process, staphylococcal protein A (SPA) was added to targetedly bind the Fc segment of the monoclonal antibody. The immunosensor showed a low detection limit (LOD) of 0.0013 ng/mL, a linear range of 0.01–100 ng/mL, and recoveries ranging from 92.77 to 100.02% in pork and 92.26–101.23% in milk. Furthermore, the immunosensor exhibited good stability, reproducibility, and specificity in detecting TMC in pork and milk real samples.
研究领域:
分析化学
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食品科学
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纳米技术
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Alan Meng,Xiangcheng Yuan,Zhenjiang Li,Kun Zhao,Liying Sheng,Qingdang Li
A binder-free, rapid and low-cost electrodeposition technology for growing 3D porous (Ni-Co) 3 S 4 nanosheets arrays on glassy carbon electrode (GCE) functionalized by reduced graphene oxide-poly (3,4-ethylenedioxythiophene) (rGO-PEDOT) hybrid film is successfully developed to construct a sensor for non-enzymatic glucose sensing. The main experimental variables affected on the electrochemical performance are optimized. Benefiting from the synergistic effect of the new active material system, the unique morphology and the strong combination among (Ni-Co) 3 S 4 , rGO-PEDOT and bare electrode, the sensor constructed under the optimal conditions manifests wide detection range (1–5000 μM), highly sensitivity (938.4 μA mM −1 cm −2 ), rapid response (<1.5 s) and low detection limit (0.503 μM). Additionally, the sensor exhibits outstanding selectivity, stability and reproducibility. Significantly, the detection results of the sensor for glucose in human blood serum are well consistent with that in hospital. This research work opens an attractive strategy to construct nanocomposites, and provide a new insight into the development of the non-enzymatic glucose biosensor with high performance.
研究领域:
材料科学
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电化学
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纳米技术
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生物传感器
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Xueyan Wang,Baoyu Huang,Xiaofang Wu,Ding Gu,Xiaogan Li
Two-dimensional reduced graphene oxide (rGO) nanosheets were pre-sulfonated and further incorporated by WS 2 nanoflakes to form S-rGO/WS 2 heterojunctions by hydrothermal synthesis. The sulfonation and WS 2 incorporation in S-rGO/WS 2 heterojunction have been confirmed by EDS, XPS and FTIR. The sensing performance of rGO-based chemiresistive-type sensor to ammonia at room temperature has been significantly improved by the sulfonation and incorporation of WS 2 nanoflakes. The chemiresistive sensor based on S-rGO/WS 2 exhibits enhanced response signals to 10∼50 ppm ammonia, while the response/recovery time is significantly shortened compared to rGO/WS 2 reported previously at room temperature. It benefits from both the extra introduced active acid centers sensitive to ammonia molecules and the better desorption-ability facilitated significantly by sulfonate groups (S O 3 H). The adsorption energy calculation of ammonia molecule on the sulfonated graphene sheet based on density functional theory (DFT) has been performed to illustrate the adsorption capacity of S O 3 H group. The selectivity and stability of gas sensor based on S-rGO/WS 2 have also been studied.
研究领域:
材料科学
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化学传感器
·
纳米技术
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）