JOURNAL OF NANOBIOTECHNOLOGY
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2025
·Vol. 23
·期 1
·1-20
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IF 12.6
Shen Jiayi,Luo Yuwen,Hu Wuming,Zhang Susu,Yang Yu,Lan Linwen,Liu Enqian,Xu Jian,Hu Tingting,Nie Jingjun,Chen Dafu,Liang Ruizheng,Lyu Lingchun
Myocardial ischemia/reperfusion (I/R) injury represents a major global health concern, driven by excessive reactive oxygen species (ROS), which closely correlates with subsequent myocardial infarction and fibrosis. Combining ROS scavenging with ischemic myocardium revascularization holds therapeutic promise. However, developing multifunctional therapeutic agents that can simultaneously scavenge ROS and promote angiogenesis remains unreported. Herein, we propose MgSrCeAl-layered double hydroxide (LDH) nanosheets as a multifunctional ROS scavenger and angiogenesis promoter for efficient myocardial I/R injury treatment. MgSrCeAl-LDH nanosheets not only exhibit exceptional hydroxyl radicals (·OH) and superoxide anions (·O 2 − ) scavenging capabilities, which is 1.78 times and 1.61 times that of CeO 2 , respectively, but also respond to the ischemia-induced acidic microenvironment to release angiogenic Sr 2+ with a concentration of 32.65 ppm (pH 5.0), which is conducive to efficient angiogenesis. Specifically, the ROS levels in I/R-injured myocardium decreased to 66.2% of its original value following intervention with MgSrCeAl-LDH nanosheets, while the vascular density in the peri-ischemic myocardium increased by approximately 3.6-fold. Ultimately, the infarcted myocardial area is reduced 60.9% compared to the group without MgSrCeAl-LDH intervention. Mechanistically, MgSrCeAl-LDH nanosheets alleviate myocardial infarction and fibrosis caused by I/R injury through activating PI3K/Akt pathway and inhibiting TGF-β pathway, ultimately improving cardiac function and inhibiting ventricular remodeling. Graphical abstract
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DALTON TRANSACTIONS
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2025
·
IF 3.3
Lihua Wang,Xionghongcan Zhou,Zengyu Ye,Sha Li,Yayang Wu,Kaiyue Song,Feng Zhao,Xianglong Li
Developing smart nanosystems that integrate controlled drug release with gas therapy represents a promising strategy to enhance chemotherapeutic efficacy while minimizing systemic toxicity. Herein, we report a biodegradable in-situ hydrogen sulfide (H2S) nanogenerator based on ZnS nanoparticles (NPs) for pH-responsive co-delivery of H2S and doxorubicin (DOX). ZnS NPs were synthesized via a rapid hydrothermal method and subsequently functionalized with hyaluronic acid (HA) to form ZnS@HA, which was then electrostatically loaded with DOX to yield ZnS@HA-DOX NPs. The nanosystem maintains structural integrity under physiological conditions (pH 7.4) but selectively releases both DOX and H2S under acidic tumor microenvironment (pH 5.0), as confirmed by fluorescence spectroscopy, lead acetate paper assays, and WSP-1 probe-based quantification. In vitro studies using 4T1 breast cancer cells revealed that ZnS@HA-DOX NPs exhibit significant cytotoxicity compared to ZnS@HA NPs alone, indicating a synergistic effect between H2S gas therapy and chemotherapy. This work demonstrates the potential of ZnS-based nanogenerators as a multifunctional platform for pH-triggered, site-specific, and synergistic cancer therapy.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2025
·
IF 9
Junjing Li,Xingzhou Mu,Di Wu,Ganhua Liu,Liang Wang
The development of efficient electrochemical coupling systems for persulfate activation is crucial for expanding the treatment of pollutants such as pharmaceuticals and personal care products (PPCPs) in wastewater. In this study, we present an electrically activated persulfate-coupled N-doped graphene membrane electrode system, which achieves complete (100 %) removal of chlortetracycline hydrochloride (CTC) within 90 min. Nitrogen doping significantly enhances the catalytic activity of graphene membrane electrodes for PMS activation, improving CTC degradation by 45 %. X-ray photoelectron spectroscopy (XPS) identified pyridinic nitrogen and graphitic nitrogen as the dominant PMS-active sites. Free radical quenching experiments and electron spin resonance (ESR) spectroscopy elucidated the reaction mechanism. Under an external electric field, the N-doped graphene membrane electrode functions as an anode, triggering PMS activation and generating reactive oxygen species (ROS), including SO 4 •- , •OH and 1 O 2 . Furthermore, high-performance liquid chromatography-mass spectrometry (HPLC-MS) was employed to analyze intermediate products and assess the toxicity of CTC. The system exhibited excellent pH adaptability and broad applicability for PPCPs removal. This innovative electrochemical coupling system with N-doped graphene membrane electrodes advances the prospects of electrochemical technology in water pollution control. Environmental Implication In this study, we propose an innovative “electrochemically activated persulfate coupled N-doped graphene membrane electrode system” strategy. Using this strategy, we successfully fabricated a graphene membrane electrode material with N-active sites for persulfate activation. The N-sites in this structure enhance the charge distribution, electronic density, and spin density of the graphene material, thereby facilitating the activation of persulfate. The electrode material not only exhibits facile synthesis, excellent activity, and high stability, but also demonstrates considerable promise for applications in the field of environmental remediation.
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Biosensors-Basel
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2025
·Vol. 15
·期 10
·700
·
IF 5.6
Yu Qin,Jiali Xie,Shujun Zhen
Fluorescence anisotropy (FA) has been widely used for analyzing biomolecules due to its high throughput, homogeneous detection, and strong resistance to photobleaching. However, the traditional FA method suffers from low sensitivity when the target molecules are small and rotate rapidly, often producing insignificant changes in the FA value. In this study, by combining double signal amplification through the trans-cleavage of CRISPR-Cas12a and DNA tetrahedron assemblies with a large molecular size, a new, fast, simple and highly sensitive FA method was constructed to achieve the quantitative detection of hepatitis B virus DNA (HBV-DNA). The experimental results showed that the linear range of this method was 0.5–9 nmol/L, and the detection limit (LOD = 3σ/k) was 48 pmol/L. In addition, the method demonstrated excellent selectivity and anti-interference, and it was successfully applied to detect HBV-DNA in human serum, indicating that this method has the potential for clinical diagnosis.
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DESALINATION
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2025
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IF 9.8
Yuxin Mou,Ziyi Yuan,Feng Li,Yuting Di,Mingduo Yao,Deyin Hou
This study systematically investigated the effects of three representative surfactants, SDS (anionic), DTAC (cationic), and Brij L23 (nonionic), on membrane wetting and fouling in DCMD using PVDF, PTFE, and omniphobic membranes. These surfactants were chosen because they represent the major classes with distinct headgroup chemistries and charge states, allowing comprehensive evaluation of electrostatic, hydrophobic, and micellar interactions. Results showed that PVDF membranes experienced severe wetting and fouling, particularly with Brij L23, while omniphobic membranes maintained strong anti-wetting performance and minimal fouling. ATR-FTIR and SEM analyses confirmed surfactant penetration and fouling layer formation on PVDF, in contrast to the clean porous structures of omniphobic membranes. XDLVO calculations indicated that Lewis acid-base interactions were the dominant driving force, and membranes with higher interfacial energy barriers exhibited better fouling resistance. This study uniquely integrates counterion effects with mechanistic insights while systematically evaluating different surfactant categories and membrane types, thereby establishing a novel framework for the rational design of fouling-resistant membranes in sustainable water treatment.
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ANALYTICA CHIMICA ACTA
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2025
·
IF 6
Zhouzhou Zhu,Xiangfu Hu,Jiahao Pan,Zhenda Lu
Background Electrochemiluminescence (ECL) imaging is a highly promising analytical technique offering ultra-sensitivity and spatiotemporal resolution for biosensing and environmental monitoring. However, trace-detecting based on ECL remains challenging due to inherently low signal intensity caused by stability limitations and poor luminescence efficiency. Microelectrodes (MEs) with three-dimensional (3D) dendritic structure integrate the advantages of large-surface-area and field-enhancing. Nevertheless, research specifically applying such MEs to ECL imaging remains scarce, which obstructed the development of optimized ECL detection systems. This work addresses this by developing a novel dendritic ME array for enhanced ECL detection. Results In this study, a dendritic microelectrode (ME) array was fabricated using Ag nanoparticle seeds integrated with nanoprinting, seed-mediated growth and electrodeposition. This approach enabled precise spatial and morphological control of nanoparticle growth, optimizing the 3D dendritic hierarchical architecture. The dendritic MEs demonstrated a three-order-of-magnitude enhancement in electrochemical catalytic efficiency compared to initial structures, while exhibiting equivalent ECL intensity amplification versus submicron and ITO electrodes under identical conditions. Comprehensive characterization confirmed exceptional response to ECL markers such as Ru(bpy) 3 2+ and high-performance glucose detection with a linear range of 0.1–10 mM, low detection limits, and high selectivity. Furthermore, ME array exhibited remarkable selectivity in detecting glucose concentrations amidst various interfering substances. The platform concurrently enables visualization of local analyte concentration gradients. Significance This work advances the fundamental understanding of ME design for signal amplification while providing a versatile platform for ultrasensitive electrochemical sensors. The findings offer key insights for future microelectrode development, demonstrating that incorporating 3D microstructures directly onto electrode surfaces is a highly effective strategy for amplifying ECL signals particularly for trace analytes. This approach opens new avenues for applications in bioanalysis and environmental monitoring.
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JOURNAL OF ALLOYS AND COMPOUNDS
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2025
·
IF 6.3
Yanning Qu,Wanquan Ma,Shihu Lei,Yuan Yu,Dongfeng Sun,Huayun Du,Madi Ren
Metal-organic frameworks (MOFs)-based heterojunctions, as one of porous materials, offer abundant unsaturated coordination sites, making them suitable for photocatalytic N 2 reduction. However, complicated reaction methods, mismatched interfaces and ineffective photo-induced carrier separation restricted their applications. In this study, a kind of Cu 2 O/Cu-MOF heterojunction was synthesized via a fast in situ method in acidic medium (CCM-a). It showed a typical core-shell structure with Cu-MOF coating surrounding the Cu 2 O. The photocatalytic NH 3 yield of CCM-a reached 83.25 μmol/g/h, which was 9.03 and 19.64 higher than that of pure Cu 2 O and CCM-b, respectively. The reaction pathway of N 2 reduction was investigated using density functional theory (DFT) and in-situ FT-IR spectra, revealing a preference for the alternating association pathway. The photocatalytic mechanisms were analyzed through the built-in electric field (IEF) theory, supporting its S-scheme charge-transfer route of CCM-a. The novel material achieved from in situ strategy not only ensured a good interface matching ability but also facilitated sufficient separation of photo-generated carriers via a S-scheme route for efficient photocatalytic N 2 fixation.
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JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
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2025
·
IF 4.9
Haohao Zhang,Mingyuan Pang,Min Yang,Zhen Kong,Jiajia Ye,Wensi Li,Yen Leng Pak,Juan An,Koulong Wu,Jibin Song
Chemotherapy, as a primary cancer treatment modality, faces limitations in efficacy and patient tolerance due to non-specific cytotoxicity, drug resistance, and adverse effects. This study develops a multifunctional nanoplatform (DOX-FA-CS-BiO 1 . 5 ) for NIR-Ⅱ photoacoustic imaging-guided visualized synergistic photothermal-chemotherapy, aiming to achieve enhanced therapeutic outcomes. The BiO 1 . 5 component ensures superior photothermal conversion efficiency and photoacoustic imaging performance, while the synergistic platform's high drug loading capacity and pH-responsive controlled release profile significantly improve tumor cell eradication rates. The chitosan-folic acid dual coating enhances biocompatibility and tumor-targeting specificity. Through synergistic effects, this nanoplatform demonstrates superior antitumor efficacy, offering a novel paradigm for constructing integrated platforms for imaging-guided visualized cancer theranostics.
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TALANTA
·
2025
·
IF 6.1
Muhammad Hussnain Afzal,Wajeeha Pervaiz,You Xu,Nomair Elahi,Zhuo Huang,Guangfang Li,Hongfang Liu
The widespread use of acetaminophen (AP) has resulted in its frequent presence in pharmaceutical waste, posing ecological and health risks. To address this, a voltametric sensor based on a bimetallic UiO-66-NH 2 metal-organic framework incorporating Zirconium (Zr) and Cerium (Ce) was developed for sensitive AP detection. The incorporation of Ce 3+ not only introduced redox active sites but also reduced the particle size from 495-650 nm in (Zr)UiO-66-NH 2 to 230–365 nm in (Zr/Ce)UiO-66-NH 2 , increasing surface area and enhancing electrocatalytic activity. The combined synergistic effect of Zr and Ce significantly improved charge transfer and enhanced oxidation current response compared to the unmodified GCE. The sensor exhibited excellent electrocatalytic performance with a wide linear range (0.072–680 μM), high sensitivity (0.496 μAμM −1 cm −2 ), and a low limit of detection (0.024 μM). In addition, the sensor exhibited excellent selectivity against interfering species and maintained stable performance over 21 days. The sensor's practical applicability was validated by testing real environmental samples, achieving recovery rates of 97–103 %, and the results were consistent with those obtained using high-performance liquid chromatography (HPLC).
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Scientific Reports
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2025
·Vol. 15
·期 1
·1-10
·
IF 3.9
Mo Fei,Hong Min,Chen Xiao,Wang Qiye,Dong Chao
The accumulation of regulatory T cells (Tregs) in the tumor microenvironment is a key factor for immunosuppression and immune escape of cancer cells. Genistein is a natural isoflavonoid in soybeans, possessing a wide range of pharmacological bioactivities. However, the immunomodulatory potential of genistein remains largely unexplored. This study investigated the synergistic efficacy of genistein with anti-PD-1 therapy in a mouse model of melanoma. The regulatory role of genistein in Treg differentiation and functional stability was also examined in this study. The antitumor effects of genistein, alone or in combination with anti-PD-1 therapy, were evaluated in the mouse B16-F10 melanoma model. The profiles of intratumoral Tregs and CD8 + cytotoxic T cells were profiled using flow cytometry. The impact of genistein on the suppressive function and differentiation of Tregs was also studied in vitro. RT-qPCR, Western blotting and flow cytometry were conducted to characterize the functional phenotype of Tregs under genistein treatment. Genistein showed a synergistic effect with anti-PD-1 therapy in B16-F10 melanoma models, achieving enhanced immune infiltration and functional reprogramming of the tumor microenvironment. This combinatorial strategy not only reduced intratumoral Tregs but also elevated the CD8 + /Treg ratio. At the mechanistic level, genistein effectively suppressed critical Treg surface markers, which are indispensable for Treg-mediated immunosuppression. Furthermore, it disrupted Treg differentiation by attenuating PI3K/AKT signaling phosphorylation. Our data demonstrated that genistein boosts the antitumor effect of anti-PD-1 therapy by impairing the immunosuppressive function and differentiation of Tregs, indicating that genistein has the potential to enhance the treatment outcome of the immune checkpoint inhibitor.
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CHEMICAL ENGINEERING JOURNAL
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2025
·
IF 13.2
Yi Yuan,Xia Zhang,Qiyong Guo,Bing Li,Lin Li,Shaoyun Wang,Qingrong Xue,Jintao Liu
Dietary bioactives and their combinations hold considerable promise for alleviating ulcerative colitis (UC). However, their use is often constrained by poor solubility, low bioavailability, and rapid clearance. To address these limitations, we developed a dual-targeted, food-grade Pickering emulsion engineered to respond to the gastrointestinal environment for improved dietary intervention in UC. Specifically, fish oil Pickering emulsions were stabilized with curcumin (Cur)–loaded shellac nanoparticles (CLSNPs) in combination with chitosan (CS). CLSNPs effectively encapsulated Cur and stabilized the oil–water interface through interactions with CS. The resulting Pickering emulsion remained highly stable in simulated gastric fluid but transformed into an emulsion gel in simulated small intestinal fluid via CS gelling. This transition prevented premature digestion and ensured safe delivery to the colon. In the colon, gut microbiota degraded the gelled emulsion, enabling the controlled release of fish oil and CLSNPs. The intrinsic negative charge of CLSNPs promoted selective binding to positively charged inflammatory sites, enhancing the therapeutic action of Cur, while fish oil contributed to restoring gut microbiota balance. In a dextran sulfate sodium–induced UC mouse model, this emulsion system significantly improved intestinal barrier repair, immune modulation, and gut microbiota regulation. Collectively, these results demonstrate that such custom-designed, food-grade Pickering emulsions provide a promising dietary intervention, with potential to complement or even replace conventional UC therapies.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Qi Liang,Tao Liao,Liqin Zhu,Changxiang Liu,Qian Liu,Jianhua Xiong,Ruiping Deng,Xiaoning Liao
A novel nanocomposite consisting of core (Cu)-shell (Ag) nanoparticles encapsulated in carbon nanotubes (CTs) grown on a MOF-derived carbon framework was synthesized by in-situ pyrolyzing method. After further modification with multi-walled carbon nanotubes (MWCNTs), a hierarchical porous carbon network confined core (Cu)-shell (Ag) bimetal (Cu@Ag@CTs/MWCNTs) with excellent catalytic properties to isoproturon (ISO) and norfloxacin (NOR) was obtained. The electrochemical sensor based on the Cu@Ag@CTs/MWCNTs can simultaneously detect the ISO and NOR with excellent sensitivity, good selectivity and perfect stability as well as a separation of peak potential of 270 mV. The calibration curves for ISO and NOR achieved by the proposed sensor range from 80 nM to 18 μM and 7.0 nM to 1.7 μM with detection limits of 26 nM and 2.0 nM, respectively. Moreover, the sensor shows good recoveries in simultaneous detection of ISO and NOR in real sample analysis, highlighting its high feasibility in practical application.
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