Microbial Cell Factories
·
2024
·Vol. 23
·期 1
·1-14
·
IF 4.3
Song Yuping,Lv Hongfa,Xu Lei,Liu Zhiying,Wang Jianfeng,Fang Tianqi,Deng Xuming,Zhou Yonglin,Li Dan
Background Inorganic polyphosphate (polyP)-targeted polyphosphate kinase 1 (PPK1) has attracted much attention by virtue of its importance in bacterial pathogenicity and persistence, as well as its exclusive presence in microorganisms. However, only very few drugs have been found to be efficacious in inhibiting the Acinetobacter baumannii (A. baumannii) PPK1 protein.Results In this study, we identified Scutellarein (Scu), a potent PPK1 inhibitor that could significantly influence PPK1-regulated motility, biofilm formation, and bacterial persistence, which was further validated by the results of transcriptome analysis. Mechanistic explorations revealed that Scu achieved its enzyme inhibitory activity predominantly through direct engagement with the active center of PPK1. Moreover, the survival rate of Galleria mellonella larvae was increased by about 35% with 20 mg/kg of Scu treatment. The remarkable therapeutic benefits of Scu were also observed in the mouse pneumonia model, shown mainly by reduced bacterial colonization, pathological lesions, and inflammatory factors.Conclusion Our results revealed that Scu could attenuate the pathogenicity and persistence of A. baumannii by interfering with its important kinase PPK1.
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INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2024
·
IF 8.1
Wenhua Feng,Ze Xiao,Binyi Chen,Yunhong Pi,Changsong Hu,Wenli Zhang,Qingwei Meng,Tiejun Wang
As non-noble metal catalyst, Cu-based catalyst shows promising applying potential in the aqueous-phase reforming of methanol (APRM) due to its high dehydrogenation activity and low CO selectivity, but the poor thermal and hydrothermal stability. Herein, a Cu@S-1 catalyst with ultrasmall-2nm Cu nanoparticles size was synthesized for the first time using one-step in-situ encapsulation strategy. By the confinement effect of the zeolite Silicalite-1 (S-1) micropores, the 1 wt%Cu@S-1 catalyst exhibited absolutely superior thermal stability without using any other promoter, as well as relatively good hydrothermal stability. After 600–700 °C high-temperature treatment, no obvious aggregation of the Cu nanoparticles was found, and superior performance in the steam reforming of methanol (SRM) was maintained after running for 36 h at 300 °C. In the APRM at 210 °C, the 1 wt%Cu@S-1 catalyst showed six-fold higher hydrogen releasing rate (4.26 × 10 5 μ mo l H 2 · g Cu − 1 · h − 1 ) than the impregnated 1 wt%Cu/S-1-im catalyst, meanwhile it exhibited even better performance than the commercial 5%Pd/C catalyst. Compared with the impregnated 1 wt%Cu/S-1-im catalyst, the confinement effect of the internal pores of the S-1 for the Cu nanoparticles in the 1 wt%Cu@S-1 catalyst effectively inhibited aggregation of the Cu particles and Cu leaching in APRM, thus greatly improved the activity and hydrothermal stability.
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JOURNAL OF CHEMICAL THERMODYNAMICS
·
2025
·
IF 2.2
Xianming Zhang,Yanping Li,Yongli Wu,Yunfei Wang,Panpan Yan,Zhilei Zheng,Hongyu Peng,Yuexin Chu
Liquid–liquid equilibrium (LLE) for the binary systems of 1-octene +1-ethyl-3-methylimidazolium acetate ([EMIM][Ac]), 1-octene +1-butyl-3-methylimidazolium acetate ([BMIM][Ac]), 1-octene +1-butyl-3-methylimidazolium tetrafluoroborate ([BMIM][BF 4 ]), 2-hexanone + [EMIM][Ac], and 2-hexanone + [BMIM][Ac], and the ternary systems of 1-octene +2-hexanone + [EMIM][Ac], 1-octene +2-hexanone + [BMIM][Ac], and 1-octene +2-hexanone + [BMIM][BF 4 ] were measured at 298.15 K, 303.15 K and 313.15 K under 101.3 kPa. The solute distribution coefficient ( D ) and selectivity ( S ) were calculated to investigate the efficiencies of [EMIM][Ac], [BMIM][Ac], and [BMIM][BF 4 ] as solvents. Moreover, the temperature dependencies of D and S were investigated in this study. NRTL and UNIQUAC models were applied to correlate the experimental LLE data.
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INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2024
·
IF 8.1
Leping Cheng,Yuwei Liu,Die Li,Wenjie Xu,Jingbo Jia,Runduo Zhang,Ying Wei,Maria A. Goula,Vagelis G. Papadakis
Thermochemically upcycling waste plastics to value-added products is emerging to alleviate the waste crisis. This study aims to develop highly active and stable Ni-based catalysts for coupling pyrolysis and catalytic reforming of waste plastics to produce syngas. A series of confined NiO@S-1 catalysts with various Ni loadings (5%, 10%, 15%, and 20%) were constructed by employing dissolution-recrystallization strategy. They were in situ reduced and 15%NiO@S-1 sample showed the highest catalytic activity in terms of yield of syngas with high CO selectivity and was superior to 15%NiO/S-1 and 15%NiO/SiO 2 samples prepared by impregnation method. The TEM images of 15%NiO@S-1 catalyst after reaction showed that metallic Ni nanoparticles with average size of 10.1 nm were kept dispersive due to the confinement effect. In contrast, the apparent agglomeration of Ni nanoparticles with average size of 54.9 nm were found on the surface of 15%NiO/S-1 after reaction. This provides an effective strategy for design robust Ni-based confined catalysts for thermochemically upcycling waste plastics.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Yanyan Xing,Mengke Tian,Ruoman Ji,Ting Wang,Xiaohong Hou
The immobilization of bovine serum albumin (BSA) with high efficiency, good reproducibility and stability for chiral separation has attracted much attention in the medicinal field. BSA was immobilized into MIL-101(Cr) by three ways for efficient chiral separation in this research. MIL-101(Cr)@BSA was firstly prepared by adsorption, NH 2 -MIL-101(Cr) ⊂ BSA by direct bonding, and NH 2 -MIL-101(Cr) ⊂ Glu ⊂ BSA by glutaraldehyde crosslinking bonding. Compared to the other two materials, NH 2 -MIL-101(Cr) ⊂ BSA as a new enantioselective separator, with the most BSA loading rate of 437.6 mg g −1 exhibited excellent chiral separation performances and succeeded in realizing a complete chiral separation of racemic 1–phenylethanol within 14 s. It was found that the NH 2 -MIL-101(Cr) ⊂ BSA was more strongly bound to R -1-phenylethanol and the ee value could reach 100 % when the material was added in three batches. The different interactions between BSA and racemates mainly including hydrogen bonding, hydrophobic interaction and π-π interaction were revealed by the molecular docking studies, especially hydrogen bonding, accounting for the observed selective chiral recognition capacity of racemic 1–phenylethanol of NH 2 -MIL-101(Cr) ⊂ BSA. Moreover, both of NH 2 -MIL-101(Cr) ⊂ BSA and NH 2 -MIL-101(Cr) ⊂ Glu ⊂ BSA showed excellent stability and reusability. This combination of theory and experiment provided a clearer basis for other researchers to have different requirements for protein immobilization, and provided new ideas and methods for the development of chiral selectors.
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JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Lisong Xia,Lei Cheng,Wenni Xi,Xiliang Zhang,Xianyang Shi
The environmental fate and risks of mononitrophenols (mono-NPs), the simplest nitrophenols (NPs) often found in aquatic environments, are profoundly influenced by anaerobic bioreduction and co-existing electron shuttles (ESs), but little is known about the underlying mechanisms. Here, we elucidate the pathways of anaerobic mono-NPs bioreduction by Shewanella oneidensis MR-1 and assess the effect of model ESs on these processes. We found that all three mono-NPs isomers could be readily reduced to their corresponding aminophenols by S. oneidensis MR-1 under anaerobic conditions. CymA, a core component of the Mtr respiratory pathway, performs a dynamic role in these bioreduction, which is highly dependent on the bioreduction kinetics. The exogenous addition of quinones was found to accelerate the mono-NPs bioreduction through interactions with key outer-membrane proteins (e.g., OmcA and MtrC), and all these processes matched well to linear free energy relationships (LFERs). Surprisingly, adding riboflavin did not influence the bioreduction of all three mono-NPs isomers, which may be due to the contribution of OmcA and MtrC to these bioreduction processes and their downregulated expression. This study enhances our understanding of the environmental fate of mono-NPs and their bioconversion processes, providing valuable insights for the bioremediation of nitrophenol-contaminated sites.
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POLYMER COMPOSITES
·
2024
·Vol. 45
·期 10
·8770-8780
·
IF 4.8
Weixing Sun,Guixiang Liu,Xinpeng Fan,Shuangquan Liao
Improving the interfacial interaction between silica and rubber is always a key issue to be solved for silica-filled rubbers. In this paper, composites are prepared by an in-situ modification method using 1-ethylpyridinium bromide ([EPy]Br) as the raw material, and the effect of its dosage on the interfacial interactions and macroscopic properties of silica-filled natural rubber (NR) composites is investigated. The effect of [EPy]Br on silica dispersion and interfacial interaction between silica and NR is verified through rheological tests and calculations of glass layers. Compared with the original samples, composite performance is optimized when the usage amount is at 1phr which increases tensile strength by 5 MPa, enhances abrasion resistance by 24%, and shortens optimum curing time in half. This study is expected to provide a new strategy for improving the processability of silica-containing composites while reducing energy consumption. Highlights [EPy]Br enhances the interaction between rubber and filler while modifying the surface of silica. Increasing the dosage of [EPy]Br leads to a reduction in the Payne effect. The addition of [EPy]Br enhances the dispersion of silica in natural rubber. [EPy]Br significantly reduces the duration of the induction period for vulcanization. The dosage of [EPy]Br allows for controlling the mechanical properties of NR/SiO 2 .
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ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Shuai Ma,Xiaohua Zhang,Jie Zhang,Xiaohui Zhang,Yu Liu,Chenglong Yu,Zhenxing Yue
Low-loss microwave dielectrics are of significant importance for the miniaturization and integration of microwave devices. In this paper, the ceramics of nominal composition Mgn+1TinO3n+1 (n = 3–6) are synthesized, and the correlations among their phase compositions, defect behaviors, and microwave dielectric properties are systematically investigated. The analyses indicate that the Mgn+1TinO3n+1 ceramics are a biphasic system consisting of hexagonal ilmenite-structured MgTiO3 and cubic spinel-structured Mg2TiO4. The Rietveld refinement results demonstrate that as the n value increases, the content of the MgTiO3 phase gradually increases, while that of the Mg2TiO4 phase decreases. The results of the thermally stimulated depolarization current (TSDC) indicate that the oxygen vacancy-related defects are considered to be the main types of defects in Mgn+1TinO3n+1. As the value of n increases, the increase of the MgTiO3 phases with a more stable crystal structure leads to a gradual decrease in the concentration of oxygen vacancies. The Mgn+1TinO3n+1 ceramics exhibit excellent microwave dielectric properties at n = 6: εr = 17.55, Q × f = 284,600 GHz, and τf = −46.39 ppm/°C. In addition, the relationship between the dielectric constant and the electric field distribution is solved utilizing high-frequency structure simulator (HFSS) software, and the Q × f values are also predicted. The low-frequency dielectric temperature spectra and direct current pulse charge/discharge tests show that the low-loss Mgn+1TinO3n+1 ceramics can be good candidates for microwave capacitors even when operated under high-temperature conditions.
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INORGANIC CHEMISTRY
·
2025
·
IF 4.7
Yong Song,Wenjia Hao,Jinsong Li,Bo Jin,Rufang Peng
Hierarchical porous metal–organic frameworks (HP-MOFs) have attracted considerable attention because of their hierarchical pores, which can address the slow mass transfer and less exposure of active sites in pristine microporous MOFs. Although several preparation methods have been developed to date, a large-scale technique for the synthesis of HP-MOFs is still lacking. In this study, we report a novel method for the large-scale synthesis of HP-HKUST-1 based on liquid-assisted spiral gas–solid two-phase flow (LA-S-GSF). This method ingeniously uses a nebulizer to promote the rapid synthesis of nano-HKUST-1 by introducing trace amounts of water during the S-GSF reaction. During the washing and drying process, these nanoparticles were fully fused to form nanocrystalline aggregates, resulting in a hierarchical porous structure with a large number of micropores, mesopores, and macropores. The pore size distribution can be regulated by changing the drying temperature to obtain HP products with combinations of micropores and mesopores, micropores and macropores, and the formation mechanism of the HP structure was also explored. This method required only 11 min of reaction time to obtain 25.2 g of HP-HKUST-1 in 96% yield, with a corresponding space-time yield (STY) of 6.9 × 104 kg m–3 day–1.
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ANALYST
·
2011
·Vol. 136
·期 22
·4770-4773
·
IF 3.913
Ling Chen,Tingting Lou,Chunwei Yu,Qi Kang,Lingxin Chen
An approach for mercury ions (Hg2+) sensing based on the Hg2+-induced aggregation of thymine (T)–SH-functionalized gold nanoparticles (AuNPs) has been reported. The T–SH ligands that we synthesized can easily be coupled to the surface of AuNPs through the Au–S bond and can recognize Hg2+ with high selectivity by forming a T–Hg–T complex with strong affinity. For the T–SH-functionalized AuNPs (T–S-AuNPs) sensor, upon addition of Hg2+, the formation of the T–Hg–T complex induces aggregation of T–S-AuNPs and results in a significant change of color and UV-Vis absorption spectra. Thus, our method can be used for the rapid, easy and reliable screening of Hg2+ in aqueous solution, with high sensitivity (2.8 nM) and selectivity over competing analytes. The developed method is successfully applied to the sensing of Hg2+ in real environmental samples.
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2024
·
IF 6.2
Wenmiao He,Zhiwei Liu,Hongchao Zhang,Qian Liu,Zhenkun Weng,Dongmei Wang,Wenhui Guo,Jin Xu,Dayong Wang,Zhaoyan Jiang,Aihua Gu
Bisphenol S (BPS) is widely presented and affects aging with unclear mechanisms. Here, we applied C. elegans to evaluate the effects of BPS on lifespan and healthspan and to investigate the underlying mechanisms. Both early-life and whole-life exposure to BPS at environmentally relevant doses (0.6, 6, 60 μg/L) significantly decreased lifespan, and healthspan (body bend, pharyngeal pumping, and lipofuscin accumulation). BPS exposure impaired mitochondrial structure and function, which promoted ROS production to induce oxidative stress. Furthermore, BPS increased expressions of the insulin/IGF-like signaling (IIS). Also, BPS inhibited expression of the IIS transcription factor daf-16 and its downstream anti-oxidative genes. Quercetin effectively improved BPS-induced oxidative stress byreversing BPS-regulated IIS/ daf-16 pathway and anti-oxidative gene expressions. In daf-2 and daf-16 mutants, the effects of BPS and quercetin on lifespan, healthspan, oxidative stress, and anti-oxidative genes expressions were reversed, demonstrating the requirement of IIS/ daf-16 for aging regulation. Molecular docking and molecular dynamics simulations confirmed the stable interaction between DAF-2 and BPS mainly via three residues (VAL1260, GLU1329, and MET1395), which was attenuated by quercetin. Our results highlighted that adverse effects of BPS on impairing lifespan and healthspan by affecting IIS/ daf-16 function against mitochondrial stress, which could be inhibited by quercetin treatment. Thus, we first revealed the underlying mechanisms of BPS-induced aging and the potential treatment.
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ACS Biomaterials Science & Engineering
·
2025
·
IF 5.5
Yang Zhang,Han Wang,Ke Liu,Ruimeng Sun,Yurou Wang,Jiayu Guo,Wenxiang Zhou,Haoran Zheng,Yanfei Qi
Acute renal injury (AKI) has a high incidence rate and mortality, but current treatment methods are limited. As a kind of nanomaterial with enzyme-like activity, nanozyme has shown outstanding advantages in treating AKI according to recent reports. Herein, we assess the potential of manganese-based nanozymes (MnO2–BSA NPs) with excellent biosafety in effectively alleviating AKI. Our findings in vitro and in vivo reveal that MnO2–BSA NPs exert regulatory effects on oxidative stress, inflammation, and apoptosis. These effects are mediated through activation of the Nrf2/HO-1 and PI3K/Akt/NF-κB pathways. Notably, it was observed that the cytoprotective effect of MnO2–BSA NPs is abrogated upon inhibition of Nrf2 expression, highlighting the important role of this transcription factor in cellular protection. In summary, the study demonstrates the protective effect of MnO2–BSA NPs in AKI and provides the molecular mechanisms involved, which can contribute to the advancement of potential therapeutic interventions for nanozyme-based treatments.
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