Agriculture-Basel
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2023
·Vol. 13
·期 12
·2192
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IF 3.6
Hao Zhu,Wendi Cao,Yicai Huang,Niel A. Karrow,Zhangping Yang
Pyocyanin (PCN) is an extracellular toxin secreted byPseudomonas aeruginosa(PA), which has redox capacity and disrupts the redox balance of host cells, affecting cell function and leading to cell death. The aim of this experiment was to compare the degree of apoptosis, inflammation, and oxidative stress of bovine mammary epithelium cells (bMECs) induced by lipopolysaccharide (LPS) and pyocyanin (PCN) and to examine whether PCN can promote the apoptosis, inflammation, and oxidative stress of bMECs induced by LPS. In this study, 1 µg/mL LPS and 1 µg/mL PCN were finally selected for subsequent experiments through dose-dependent experiments. In this study, cells were not given any treatment and were used as the control group (NC). The cells were treated with PCN or LPS individually for 6 h as the PCN group (PCN) or the LPS group (LPS), and the combination of LPS and PCN challenge for 6 h as the LPS + PCN (LPS + PCN) group. Compared with the control and LPS groups, PCN resulted in a significantly upregulated expression of genes related to pro-inflammatory (IL-6, TNF-α, MyD88), apoptotic (Bax, Caspase3, Caspase9), as well as protein expression of components in the TLR4/NF-κB signaling pathway (TLR4, p-p65, p65), and p53 signaling pathway (p-p53, p53, Caspase9) (p< 0.05). Moreover, the expression of genes and proteins was significantly upregulated after PCN treatment combined with LPS compared to either LPS or PCN challenge alone (p< 0.05). The stimulation of PCN combined with LPS significantly increased reactive oxygen species (ROS) and malondialdehyde (MDA) production in bovine mammary epithelial cells (bMECs), as well as decreased glutathione peroxidase (GSH-Px) and total antioxidant capacity (T-AOC). Moreover, cells in the LPS + PCN group aggravated oxidative stress and antioxidant inhibition in cells. In addition, the expression of the corresponding genes and proteins related to the Nrf2 pathway (Nrf2, HO-1) was significantly down-regulated in the PCN group as compared to the control group (p< 0.05). Altogether, PCN stimulation exacerbates inflammatory reactions, apoptosis, and oxidative stress reactions, as well as when combined with LPS challenge in bMECs. Therefore, this study indicates that PCN manifests a role in promoting apoptosis, inflammation, and oxidative stress and interacting with LPS to enhance more serious biological stress responses.Keywords:pyocyanin;LPS;apoptosis;cellular inflammation;oxidative stress
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Journal of Biological Engineering
·
2023
·Vol. 17
·期 1
·1-11
·
IF 5.6
Karimi Hajishoreh Negar,Dadashpour Mehdi,Akbarzadeh Abolfazl
Due to their outstanding structures and properties, three-dimensional (3D) hydrogels and nanoparticles have been widely studied and indicated a very high potential for medical, therapeutic, and diagnostic applications. However, hydrogels and nanoparticles systems have particular drawbacks that limit their widespread applications. In recent years, the incorporation of nanostructured systems into hydrogel has been developed as a novel way for the formation of new biomaterials with various functions to solve biomedical challenges. In this study, alginate-loaded Zinc- laponite—curcumin (Zn/La/Cur) nanocomposites were fabricated via ionic cross-linking. The prepared nanocomposite hydrogels were characterized via FTIR and FE-SEM. Moreover, energy dispersive x-ray spectroscopy (EDX) was used to study the elements of the Zn/La/Cur nanocomposite. The NIH3T3 fibroblast cell line was utilized for the MTT assay to determine the cell viability of the fabricated alginate-loaded Zn/La/Cur nanocomposites. MTT results demonstrated that there was no evidence of toxicity in the samples. These outcomes suggest that applying Al/Zn/La/Cur nanocomposite as a biological agent could be a novel tissue engineering strategy for treating soft tissue disorders.Graphical Abstract
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DEVELOPMENTAL CELL
·
2023
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IF 11.8
Sirui Zhu,Yuanyuan Li,You Wu,Yanan Shen,Ying Wang,Yujie Yan,Weijun Chen,Qiong Fu,Yirong Wang,Xiang Yu,Feng Yu
tRNA fragments (tRFs) are a recently identified class of small noncoding RNAs. To date, the regulation of tRF abundance and its functional mechanisms have been largely unclear in plants. We investigated how the Arabidopsis thaliana receptor kinase FERONIA (FER) regulates the abundance of tRFs to inhibit global mRNA translation. We demonstrate that FER regulates tRF abundance by directly phosphorylating the tRNA-binding protein YUELAO (YL) to modulate its function. Downregulation of FER and YL prevented the modification of tRNA via cytosine-5-methylation and 2'-O-methylation, thereby increasing tRF abundance. Furthermore, we show that YL acts as an important genetic downstream target of FER signaling, and knockdown of a specific tRF partially rescues the root hair growth defects of fer and yl mutants. Our findings shed light on the abundance and regulatory mechanisms of tRF and their role in inhibiting translation in plants.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
·
IF 8.2
Jie Jiang,Mengzhang Ke,Lili Zhang,Wenwen Zhang,Weifu Dong
Developing an environment-friendly preparation method for silver nanoparticles (AgNPs) composite is significant. However, it remains challenges in size adjustment and content improvement of AgNPs. Here, the NaIO 4 oxidation and TEMPO-mediated oxidation were applied to bagasse pulp to prepare nanocellulose (NC) with both carboxyl and aldehyde groups. The aldehyde content of NC could be adjusted in the range of 0.21–1.45 mmol/g by different NaIO 4 oxidation times. When the carboxyl groups were protonated, NC with a high length-diameter ratio could construct stable hydrogels in a low concentration at 0.5 wt%. The NC hydrogels showed excellent in situ synthesis ability of AgNPs with abundant pore structure. By regulating the carboxyl group content of NC, the size distribution of synthesized AgNPs could be controlled in the range of 7.14–28.6 nm with high content of 6.79–11.0 %. The NC/AgNPs composite hydrogel exhibited high catalytic degradation activity for 4-nitrophenol and antibacterial activity . This approach for constructing NC hydrogel paves the way for AgNPs composite products with adjustable sizes and high contents.
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Foods
·
2023
·Vol. 12
·期 18
·3417
·
IF 5.2
Xiaomei Zhou,Xiaojian Gong,Xu Li,Ning An,Jiefang He,Xin Zhou,Chao Zhao
The objective of this study was to determine the antioxidant activities of defatted walnut kernel extract (DWE) and whole walnut kernel extract (WE) in vitro and in vivo. Three spectrophotometric methods, DPPH, ABTS, and FRAP, were used in in vitro experiments, and mice were used in in vivo experiments. In addition, response surface methodology (RSM) was used to optimize reflux-assisted ethanol extraction of DWE for maximum antioxidant activity and total phenolic content. The results of in vitro experiments showed that both extracts showed antioxidant activity; however, the antioxidant activity of DWE was higher than that of WE. Both extracts improved the mice’s oxidative damage status in in vivo studies. An ethanol concentration of 58%, an extraction temperature of 48 °C, and an extraction time of 77 min were the ideal parameters for reflux-assisted ethanol extraction of DWE. The results may provide useful information for further applications of defatted walnut kernels and the development of functional foods.Keywords:walnut kernel;antioxidant activity;in vitro;in vivo;response surface methodology
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MICROCHEMICAL JOURNAL
·
2021
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IF 4.821
Yuecheng Zhang,Chengjia Li,Lingbo Sun,Jie Zhang,Xiaojun Yang,Hongyan Ma
Hexavalent chromium ion (Cr(VI)) is one of the most severe environmental nonbiodegradable pollutants. Considering its high biological toxicity, it is of great significance to establish a method for Cr(VI) analysis with high sensitivity and reliability. Therefore, a simple and sensitive ratiometric sensor is developed according to the coordination between Cr(VI) and carbon dots (CDs). In the presence of Cr(VI), the original CDs photoluminescence (PL) emission at 445 nm was significantly shifted to 565 nm and gradually intensified. The coordination mechanism was investigated, the results indicated that the dual-amino groups at ortho-position of benzene ring is critical for this coordination. Under the optimal condition, a good linear relationship between the concentration of Cr(VI) and the PL intensity ratio I 565 /I 445 was obtained from 120 nM to 200 µM, with a low detection limit of 37 nM. Moreover, this method is applied into real water samples analysis with a high quality.
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ELECTROCHEMISTRY COMMUNICATIONS
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2023
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IF 5.4
Fei Zhu,Jianbang Ge,Yang Gao,Biwu Cai,Zichen Zhang,Feifei Jia,Shuqiang Jiao
Herein we reported the in-situ electrochemical concentration measurement of soluble-soluble redox species (Eu 3+ /Eu 2+ and Sm 3+ /Sm 2+ ) in molten LiCl-KCl, based on the notable natural convection in molten salts. The combined simulation and experiments confirmed the presence of strong natural convection, which resulted in steady-state current in cyclic voltammetry tests at low scan rates. Interestingly, this natural convection effects offered a novel and simple way to calculate the diffusion coefficient and concentration ratio of soluble redox species.
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CELL BIOCHEMISTRY AND BIOPHYSICS
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2023
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IF 2.6
Zheng Yilin,Wang Jun,Chen Haijun,Gao Yu
The effects of ultrasonic parameters and treatment conditions on the in vitro cellular experiments of sonodynamic therapy (SDT) have not been fully studied. Exploring the factors that affect the efficacy of SDT can provide a reference for screening effective sonosensitizers in vitro. The aim of this work is to investigate the factors that affected the SDT effects in cancer cells. Cancer cells in culture plates were exposed to ultrasound and sonosensitizers. The intracellular drug concentration was measured by using flow cytometry and the cell viability was determined by MTT assay. The SDT effects of cancer cells treated with different ultrasonic parameters under the same sonosensitizer concentration were different. The ultrasonic parameters, intracellular drug concentration, drug treatment time, cell amount, and cell status could affect the sonodynamic therapeutic effects. It is necessary to select appropriate ultrasound conditions and optimize the cellular status to make the results of the in vitro cellular experiments more reliable.
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FOOD HYDROCOLLOIDS
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2023
·
IF 10.7
Yajuan Sun,Chenglei Wang,Hang Jiang,Bingtian Zhao,Cheng Yang,Yunxing Li
Herein we reported highly biocompatible and stable oil-in-water-in-oil (o/w/o) Pickering double emulsions (PDEs) using a one-step emulsification procedure. Natural phytosterol (PS) particles and chitosan (CS) self-assembled into PS/CS complex paritlces to serve as particulate stabilizers while gelatin was used as viscosity modifier for aqueous phase. By varying the viscosity of aqueous phase, water/oil volume ratio, and homogenization time, the formation and stabilization mechanism of o/w/o PDEs were explored. The viscosity of aqueous phase and occurrence of phase inversion are essential for the formation of PDEs. Additionally, the adsoprtion of PS/CS complex particles on the oil/water interface and high viscosity of aqueous phase both aid in the stabilization of PDEs. Furthermore, the o/w/o PDEs showed good storage stability at 4 °C and 25 °C (at least 2 months), with essentially little change in emulsion structure . And a variety of oils can be used to prepare the PDEs. What's more, the o/w/o PDEs can co-encapsulate hydrophilic and hydrophobic bioactive compounds , shielding them against UV exposure and long-term storage. In summary, these findings will not only aid in the development of biocompatible o/w/o PDEs, but also provide a guidance for co-encapsulating and protecting bioactive compounds that are both hydrophilic and hydrophobic.
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MOLECULES
·
2023
·Vol. 29
·期 1
·45
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IF 4.6
Yuyue Zang,Na Hang,Jiale Sui,Senlin Duan,Wanning Zhao,Jing Tao,Songqing Li
In recent years, the utilization of biomass materials for the removal and detection of water pollutants has garnered considerable attention. This study introduces, for the first time, the preparation of Fe3O4/persimmon leaf magnetic biomass composites. The magnetic composites were employed in a magnetic solid-phase extraction method, coupled with gas chromatography-electron capture detection (GC-ECD), for the analysis of four pesticides (trifluralin, triadimefon, permethrin, and fenvalerate) in environmental water samples. The innovative magnetic persimmon leaf composites were synthesized by in situ generation of Fe3O4nanoparticles through coprecipitation and loaded onto persimmon leaves. These composites exhibit superparamagnetism with a saturation magnetization of 12.8 emu g−1, facilitating rapid phase separation using a magnetic field and reducing the extraction time to 10 min. Desorption can be achieved within 30 s by aspirating 20 times, eliminating the need for time-consuming and labor-intensive experimental steps like filtration and centrifugation. The specific surface area of the magnetic composite adsorbent increased from 1.3279 m2g−1for the original persimmon leaf to 5.4688 m2g−1. The abundant hydroxyl and carboxyl groups on the composites provide ample adsorption sites, resulting in adsorption capacities ranging from 55.056 mg g−1to 73.095 mg g−1for the studied pesticides. The composites exhibited extraction recoveries ranging from 80% to 90% for the studied pesticides. Compared to certain previously reported MSPE methods, this approach achieves equivalent or higher extraction recoveries in a shorter operation time, demonstrating enhanced efficiency and convenience. Good linearity of the target analytes was obtained within the range of 0.75–1500 μg L−1, with a determination of coefficient (R2) greater than 0.999. These findings contribute to the use of magnetic persimmon leaf biomass materials as effective and environmentally friendly adsorbents for pollutant determination in water samples.Keywords:biomass adsorbent;Fe3O4/persimmon leaf magnetic composite;magnetic solid-phase extraction;pesticides;water
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JOURNAL OF POWER SOURCES
·
2023
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IF 9.2
Tian Yan,Hao Lin,Yang Xu,Kaijie Ma,Le Liu,Jingyu Xi
In aqueous redox flow batteries (ARFBs), the redox reaction of K 4 [Fe(CN) 6 ]/K 3 [Fe(CN) 6 ] is commonly applied as an active material for the positive electrode . Its performance is restrained by inhomogeneous electrochemical activity and oxygen evolution side reactions. The activity of both the redox reaction and the side reaction lacks two-dimensional detection method. In this study, ion concentration variations on the electrode surface are converted into refractive index changes by a total internal reflection (TIR) imaging system , which enables characterization of the two-dimensional distributions of surface activity and oxygen evolution reactions (OER) onset potential on the ARFBs positive electrode. This method can obtain the two-dimensional maps of parameters of cyclic voltammetry (CV) and the onset potential of the OER. We validate the feasibility of this method by examining three different electrodes and an electrochemical stability test. This work offers a characterization tool for developing homogeneous electrodes with enhanced activity, high reversibility, and minimized OER side reactions.
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FISH & SHELLFISH IMMUNOLOGY
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2023
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IF 4.7
Ju Li,Jinmei Zuo,Xiaoyan Lv,Jiahe Ma,Xiaohong Li,Simiao Fu,Jinsheng Sun
Tissue autotomy is a unique adaptive response to environmental stress, followed by regeneration process compensating for the loss of body parts. The crustaceans present remarkable activity of appendage autotomy and regeneration, however, the molecular mechanism is still unclear. In this study, the Eriocheir sinensis Hedgehog ( EsHH ) and Smoothened ( EsSMO ) were identified in the regenerative limbs, and the function of Hedgehog signaling pathway on limb regeneration was evaluated. At the blastema growth stage of limb regeneration, the expression of EsHH and EsSMO was up-regulated in response to limb autotomy stress, and down-regulated at blastema differentiation stage. To clarify the effect of Hedgehog pathway during limb regeneration, the regenerative efficiency was evaluated with Smoothened inhibitor cyclopamine or RNAi ( ds-HH ) injection. We observed that the regenerative efficiency was significantly repressed with blockage of Hedgehog pathway at both the basal growth stage and the proecdysial growth stage, which was indicated by the delay of wound healing and blastema growth, as well as a decrease in the size of newly formed limbs. In addition, gene expression and BrdU incorporation assay showed that the proliferation and myogenic differentiation of blastema cells were suppressed with either cyclopamine or ds- HH injection. Thus, these results suggest that Hedgehog signaling pathway is essential for the establishment of limb regeneration in E. sinensis through promoting the proliferation and myogenic differentiation of blastema cells.
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