FOOD CHEMISTRY
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2025
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IF 9.8
Yujie Pan,Peini Zhao,Weili Cai,Hehe Fang,Xiaoru Wang,Junyu Zhao,Peihua Zhu,Hongmei Yang,Shenguang Ge,Jinghua Yu
Carbaryl is a broad-spectrum carbamate fungicide that may pose a threat to ecosystems and human health. To prevent and control the harm caused by excessive application of carbaryl, a full-dimensional divergence effect SERS sensor has been constructed. Biodegradable paper chips were used as sensor substrates. ZIF-8 with structural flexibility was grown in situ on carbon paper. A rough silver layer with surface like metal etching was clad outside the ZIF-8. Artificial antibody (AAB) with accurate grasping ability was polymerized on the surface of ZIF-8@Ag paper substrate. Au@Ag CSNPs were decorated on the AAB to provide abundant “hot spots”. Every functional material layer works together to form a gap and tip region with superior plasmon resonance. The proposed SERS sensor realizes the rapid and accurate on-site screening and localization of carbaryl in food with good correlation responded in the range of 0.01–20 μg·mL −1 and a low detection limit of 5.72 × 10 –3 μ g·mL −1 .
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TALANTA
·
2018
·
IF 4.244
Lingxian Ye,Guangying Zhao,Wenchao Dou
A sensitive Point-of-Care Testing (POCT) with Au-Pt bimetallic nanoparticles (Au@Pt) functionalized silica nanoparticle (SiO 2 NPs) and Fe 3 O 4 magnetic nanoparticles (Fe 3 O 4 NPs) was designed for the quantitative detection of Escherichia coli O157:H7 ( E. coli O157:H7). The poly-(4-styrenesulfonic acid- co -maleic acid) (PSSMA) as a negatively charged polyelectrolyte can be easily coated on surface of the amino group modified SiO 2 NPs via electrostatic force . PSSMA is also a good stabilizer for water-soluble bimetallic nanostructures . The PSSMA is first time used as a “bridge” to connect the negative charge Au@Pt NPs to the SiO 2 NPs, forming Au@Pt/SiO 2 NPs. Antibody and invertase conjugated Au@Pt/SiO 2 NPs (denoted as Ab/invertase-Au@Pt/SiO 2 NPs) were used as signal labels. Monoclonal antibody against E. coli O157:H7 (Ab) functionalized magnetic nanoparticles (denoted as Ab-Fe 3 O 4 @SiO 2 NPs) were used to enrich and capture the E. coli O157:H7 in positive sample. The immunosensing platform also composed of a personal glucometer (PGM) using for signal readout. Based on this sandwich-type immunoassay , the invertase in the final formed sandwich immunocomplex catalyzed the hydrolysis of sucrose to produce a large amount of glucose for quantitative readout by the PGM. Under optimal conditions, a linear relationship between the glucose concentration and the logarithm of E. coli O157:H7 concentration was obtained in the concentration range from 3.5 × 10 2 to 3.5 × 10 8 CFU mL −1 with a detection limit of 1.83 × 10 2 CFU mL −1 (3σ). This method was used to detect E. coli O157:H7 in spiked milk samples, indicating its potential practical application. This protocol can be applied in various fields of study.
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NANO
·
2018
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IF 1.1
Yongfeng Gao,Yuanhui Feng,Lu Zhou,Lucia Petti,Zhe Wang,Jun Zhou,Shusen Xie,Jian Chen,Yanping Qing
Ultrasensitive detection of alpha-fetoprotein (AFP) is critical for the early diagnosis of liver cancer. In this work, a novel surface-enhanced Raman scattering (SERS)-based immunoassay complex has been successfully developed for the detection of AFP by using the Au-Ag alloy nanoparticals and the Ag/AgBr hybrid nanostructure. As the typical bimetal or metal/semiconductor plasmonic materials, besides the strong SERS enhancement characteristics, the Au-Ag alloy nanoparticals exhibit excellent monodispersity and the Ag/AgBr hybrid nanostructure demonstrates good stability. The experimental results show that the SERS-based immunoassay of AFP presents a low limit of detection of 1.86 fg/mL and a broad dynamic range from 2 fg/mL to 0.8 μ g/mL. Furthermore, the clinical applicability of the proposed SERS-based immunoassay has been assessed by the detection of AFP in the human serum samples of cancer patient and healthy person. The test data are consistent well with that of chemiluminescence immunoassay (CLIA) in the relative errors of − 8.82–8.06% and show better detection sensitivity. It reveals that the proposed immunoassay protocol is significant for giving insight into the design of ultrasensitive biosensor and the point-of-care testing of cancers.
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MATERIALS CHEMISTRY AND PHYSICS
·
2018
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IF 2.21
Zhiming Zhang,Tingting Li,Yuqing Li,Li Yan,Libao An
Novel thiolation poly (phthalazinone ether ketone) (PPEK-SH) nanofibrous membrane (NFM) was successfully fabricated via grafting and electrospinning. The PPEK-SH NFM with high glass transition temperature (324.4 °C), robust tensile strength (12.48 MPa), high porosity (89.5%) and superior chemical resistance was employed as support of Au nanoparticles (NPs) for preparing electrocatalyst . Au NPs were deposited on the NFM via electroless plating and characterized by SEM, EDX , TEM and XRD . The as-prepared Au/PPEK-SH NFM inherited the excellent properties of PPEK-SH NFM and good conductivity of Au, thereby was successfully used as a free-standing electrocatalyst without resorting to any conductive support. The free-standing NFM exhibited excellent electrocatalytic activity towards glycerol oxidation with high oxidation peak of 74.2 mA cm −2 and low onset potential of −0.34 V. More importantly, benefiting from the strong chemical interaction between Au and -SH, Au/PPEK-SH NFM presented an incredible stability during the process of glycerol electrooxidation. Only 2.7% decrease of the peak current density was observed after 100 cycles of experiments, indicating that the free-standing electrocatalyst had great potential for long-term use. This work may provide a new approach for the development of stable free-standing electrocatalyst based on electrospun nanofibers in the application of direct alcohol fuel cells.
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ACS Applied Materials & Interfaces
·
2018
·
IF 8.097
Qian Zhao,Jiafang Piao,Weipan Peng,Yang Wang,Bo Zhang,Xiaoqun Gong,Jin Chang
Identifying the microRNA (miRNA) expression level can provide critical information for early diagnosis of cancers or monitoring the cancer therapeutic efficacy. This paper focused on a kind of gold-nanoparticle-coated polystyrene microbeads (PS@Au microspheres)-based DNA probe as miRNA capture and duplex-specific nuclease (DSN) signal amplification platform based on an RGB value readout for detection of miRNAs. In virtue of the outstanding selectivity and simple experimental operation, 5′-fluorochrome-labeled molecular beacons (MBs) were immobilized on PS@Au microspheres via their 3′-thiol, in the wake of the fluorescence quenching by nanoparticle surface energy transfer (NSET). Target miRNAs were captured by the PS@Au microspheres-based DNA probe through DNA/RNA hybridization. DSN enzyme subsequently selectively cleaved the DNA to recycle the target miRNA and release of fluorophores, thereby triggering the signal amplification with more free fluorophores. The RGB value measurement enabled a detection limit of 50 fM, almost 4 orders of magnitude lower than PS@Au microspheres-based DNA probe detection without DSN. Meanwhile, by different encoding of dyes, miRNA-21 and miRNA-10b were simultaneously detected in the same sample. Considering the ability for quantitation, high sensitivity, and convenient merits, the PS@Au microspheres-based DNA probe and DSN signal amplification platform supplied valuable information for early diagnosis of cancers.
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OPTICS COMMUNICATIONS
·
2017
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IF 1.588
Guochao Shi,Mingli Wang,Yanying Zhu,Lin Shen,Yuhong Wang,Wanli Ma,Yuee Chen,Ruifeng Li
In this work, we presented an eco-friendly and low-cost method to fabricate a kind of flexible and stable Au nanoparticles/graphene oxide/cicada wing (AuNPs/GO/CW) substrate. By controlling the ratio of reactants, the optimum SERS substrate with average AuNPs size of 65 nm was obtained. The Raman enhancement factor for rhodamine 6G (R6G) was 1.08 × 10 6 and the limit of detection (LOD) was as low as 10 −8 M. After calibrating the Raman peak intensities of R6G, it could be quantitatively detected. In order to better understand the experimental results, the 3D finite-different time-domain simulation was used to simulate the AuNPs/GO/CW-1 (the diameter of the AuNPs was 65 nm) to further investigate the SERS enhancement effect. More importantly, the AuNPs/GO/CW-1 substrates not only can provide strong enhancement factors but also can be stable and reproducible. This SERS substrates owned a good stability for the SERS intensity which was reduced only by 25% after the aging time of 60 days and the relative standard deviation was lower than 20%, revealing excellent uniformity and reproducibility. Our positive findings can pave a new way to optimize the application of SERS substrate as well as provide more SERS platforms for quantitative detection of organic contaminants vestige, which makes it very promising in the trace detection of biological molecules.
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JOURNAL OF MOLECULAR LIQUIDS
·
2018
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IF 4.513
Guo Lin,Shixing Wang,Libo Zhang,Tu Hu,Jinhui Peng,Song Cheng,Likang Fu
In this study, corn bract was modified with thiosemicarbazide (TSC-CCB) and used to selectively adsorb Au(III) from aqueous solutions. FT-IR spectroscopy, Scanning electron microscope and X-ray photoelectron spectroscopy were used to characterize TSC-CCB and investigate the adsorption mechanism. The results suggested that the maximum adsorption amount of Au(III) was 1470.22 mg/g at 25 °C with pH of 6.0. The adsorption isotherm and kinetic data were matched adequately with Langmuir model and pseudo-second-order model, respectively. Meanwhile, TSC-CCB presented a good selectivity for Au(III) from the competitive ions. The ion exchange and chelation between Au(III) and S/N groups played an important role in the enhancement of the adsorption capacity. The regeneration experiments suggested that the TSC-CCB retained high adsorption efficiency after three cycles. TSC-CCB was a promising adsorbent for recovery Au(III) from wastewater in the view of sustainability.
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ANALYTICAL CHEMISTRY
·
2018
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IF 6.042
Xilin Bai,Suying Xu,Leyu Wang
The sensitive and selective detection of hydrogen sulfide is of great importance due to its crucial role in pathological and physiological processes. Herein, we report a novel fluorescent platform, AuNCs@GC, for selective detection of hydrogen sulfide in living cells by impregnating the Au nanoclusters (AuNCs) into a biocompatible cationic polymer matrix, glycol-chitosan (GC) nanogel. The confinement effect significantly increased the emissive Au(I) units, resulting in a 6-fold enhancement of quantum yield (from 6.38% to 36.42%). In addition, the prepared positively charged AuNCs@GC nanogel exhibits excellent selectivity and improved sensitivity to aqueous sulfides. Moreover, the as-fabricated AuNCs@GC showed very good biocompatibility and super fluorescence stability across the full pH range and good salt tolerance, which demonstrated excellent adaptability toward intracellular sulfide imaging.
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Journal of Biomedical Nanotechnology
·
2018
·Vol. 14
·期 1
·150-160
·
IF 5.068
Li Taotao,Yi Huan,Liu Yuan,Wang Zunliang,Liu Shiquan,He Nongyue,Liu Hongna,Deng Yan
DNA strands have been used as templates to form different sized silver nanoclusters. Although DNA templated silver nanoclusters (NCs) probes which show outstanding fluorescence properties have been widely applied in chemical sensing and cellular imaging, synthesis of DNA template Au–Ag
bimetallic nanoclusters remains a challenge. A facile one-step synthesis method was thus developed in this study to form Au–Ag NCs using C4-ATAT-C4 as template. C4-ATAT-C4 acted as a stabilizer for preventing aggregation of the Au–Ag NCs. The obtained Au–Ag NCs stabilized
by C4-ATAT-C4 showed bright fluorescence and high stability. A series of experiments showed that temperature, citrate-citric acid buffer, sequence and concentration of DNA played an important role in the synthesis of fluorescent Au–Ag NCs. In addition, a ratiometric fluorescence probe
was designed through combining the nucleotide sequence (C4-ATAT-C4) and hybridization sequence in the presence of the double-strand-chelating dye Super SYBR Green (SG). The combination of prepared Au–Ag NCs, Super SG, and perfectly matched DNA emitted fluorescence at 500 and 590 nm,
respectively, when the composite was excited at 290 nm. A gastric cancer gene was also selectively detected by our developed ratiometric fluorescence probe.
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International Journal of Nanomedicine
·
2024
·
IF 6.6
Xinbo Zhang,Bin Xu,Jiangwei Ni,Yucheng Xiang,Zhifeng He
Purpose The chemotherapeutic agent doxorubicin (DOX) is limited by its cardiotoxicity, posing challenges in its application for non-small cell lung cancer (NSCLC). This study aims to explore the efficacy of polydopamine/Au nanoparticles loaded with DOX for chemotherapy and photothermal therapy in NSCLC to achieve enhanced efficacy and reduced toxicity.Methods Hollow polydopamine (HPDA)/Au@DOX was synthesized via polydopamine chemical binding sacrificial template method. Morphology was characterized using transmission electron microscopy, particle size and potential were determined using dynamic light scattering, and photothermal conversion efficiency was assessed using near-infrared (NIR) thermal imaging. Drug loading rate and in vitro drug release were investigated. In vitro, anti-tumor experiments were conducted using CCK-8 assay, flow cytometry, and live/dead cell staining to evaluate the cytotoxicity of HPDA/Au@DOX on A549 cells. Uptake of HPDA/Au@DOX by A549 cells was detected using the intrinsic fluorescence of DOX. The in vivo anti-metastasis and anti-tumor effects of HPDA/Au@DOX were explored in mouse lung metastasis and subcutaneous tumor models, respectively.Results HPDA/Au@DOX with a particle size of (164.26±3.25) nm, a drug loading rate of 36.31%, and an encapsulation efficiency of 90.78% was successfully prepared. Under 808 nm laser irradiation, HPDA/Au@DOX accelerated DOX release and enhanced uptake by A549 cells. In vitro photothermal performance assessment showed excellent photothermal conversion capability and stability of HPDA/Au@DOX under NIR laser irradiation. Both in vitro and in vivo experiments demonstrated that the photothermal-chemotherapy combination group (HPDA/Au@DOX+NIR) exhibited stronger anti-metastatic and anti-tumor activities compared to the monotherapy group (DOX).Conclusion HPDA/Au@DOX nanosystem demonstrated excellent photothermal effect, inhibiting the growth and metastasis of A549 cells. This nanosystem achieves the combined effect of chemotherapy and photothermal, making it promising for NSCLC treatment.
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TALANTA
·
2024
·
IF 5.6
Lvhong Gong,Shengxi Wu,Junjie Liu,Mingjun Zhang,Jinghao Zhuang,Doudou Xu
An ultrasensitive immunosensor of Cys/Au@TiO 2 based on disposable screen-printed electrodes (SPE) for PIVKA-II detection for hepatocellular carcinoma (HCC) diagnosis was developed by utilizing Cystine (Cys) and nanocomposite Au@TiO 2 . Firstly, HAuCl 4 underwent a reduction reaction with NaBH 4 , then Au nanoparticles were coated onto TiO 2 nanoparticles. Followed, Cys/Au@TiO 2 was formed through self-assembly of cysteine to allow the monoclonal antibody of abnormal thrombospondin to bound to the amino group on the surface of the composite by covalent bonding. The mechanism is to determine the changes in the current of the sensor caused by the specific binding of the abnormal prothrombin monoclonal antibody adsorbed by the complex with its antigen. The Cys/Au@TiO 2 immunosensor was fully characterized by various analytical approaches and it showed a wide linear testing range of 1–10000 pg mL −1 (R 2 = 0.991) and the limit of detection down to 0.77 pg ml −1 , with highly sensitivity and specificity. The results showed that the developed immunosensor platform can effectively detect trace amounts of PIVKA-II protein and has potent clinical application for HCC diagnosis.
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DALTON TRANSACTIONS
·
2024
·Vol. 53
·期 37
·15618-15629
·
IF 3.5
Wei Zhang,Jin Li,Cuilian Sun,Xiujing Xing,Yaokang Lv,Wei Xiong,Hao Li
Surface modification is one of the effective strategies to control the morphology and electrocatalytic performance of noble metal/transition metal oxide matrix composite catalysts. In this work, we successfully introduced modification groups such as –NH2, –COOH, and –SH on the surface of Fe3O4 using the hydrothermal method. It was found that when the modification group –COOH was introduced, the regular spherical morphology of Fe3O4 was still maintained in Fe3O4–COOH, while Fe3O4–COOH had a relatively smaller spherical particle size (≈155.9 nm). Due to its smaller particle size, Fe3O4–COOH has a larger active area than Fe3O4, exposing more active sites. The abundant active sites in Fe3O4–COOH provide more nucleation and growth sites for Au particles, which is beneficial for the recombination between Fe3O4–COOH and Au. In addition, the experimental results of exploring the effect of Au precursor dosage on the synthesis of the Fe3O4–COOH@Au structure and performance show that the synthesized Fe3O4–COOH@Au1.0 catalyst has higher electrocatalytic activity. Due to the larger electrochemically active surface area of the Fe3O4–COOH@Au1.0 catalyst compared to those of Fe3O4–COOH@Au0.5 and Fe3O4–COOH@Au1.5 catalysts, the adsorption and activation of NO2− reactants were accelerated, thereby improving the electrocatalytic performance. Therefore, owing to the morphological and structural characteristics of Fe3O4–COOH combined with the high activity of Au nanoparticles, the synthesized Fe3O4–COOH@Au exhibits effective electrocatalytic activity in the electrocatalytic NO2−RR synthesis of ammonia. At a voltage of −0.8 V (vs. RHE), the ammonia yield reached 2092.8 μg h−1 mgcat−1 and Faraday efficiency reached 81.2%. The findings of this work will enrich our understanding of the construction of efficient Fe3O4@Au catalysts based on surface functionalization and help to design efficient electrocatalytic NO2−RR catalysts for practical applications.
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