Chinese Medicine
·
2019
·Vol. 14
·期 1
·1-11
·
IF 2.265
Guo Shangxin,Lin Xiaotong,Wang Yi,Gong Xingchu
Background The discovery of bioactive compounds in traditional Chinese medicine (TCM) has become an important field in TCM modernization. Ligand fishing is a suitable method for discovery of bioactive compounds in complex mixtures such as TCM with high selectivity. Because of unique advantage of low cost and convenience, paper-based microdevices can be good carriers for enzyme immobilized ligand fishing. Methods As an important enzyme for glucose metabolism, α-glucosidase was immobilized on polycaprolactone–chitosan-modified paper to prepare the microdevice with unique microfluid structure generated by 3D printing technology, which can be easily applied to screen active compounds in herbal extracts. The preparation conditions of the paper microarray were optimized. The activity of immobilized α-glucosidase was verified by colorimetric reactions which can be easily monitored by cellphone. The paper microarray with α-glucosidase immobilized was used to screen active compounds in the water extracts of mulberry leaves and lotus leaves. Results Several key parameters including Na 2 CO 3 solution concentration, Na 2 CO 3 solution volume, glutaraldehyde concentration, crosslinking time of glutaraldehyde and time of α-glucosidase immobilization were optimized. The proposed paper-based microarray was successfully applied in screening active compounds in two herbal extracts. Four compounds including chlorogenic acid, quercetin-3- O -glucuronide, isoquercetin, and quercetin were identified as α-glucosidase inhibitors. The compounds with significant non-specific adsorption caused by chitosan, such as isoquercitrin, astragalin, quercetin, were also found to be active compounds. Conclusions An enzyme immobilized paper microarray was designed and fabricated in this work. Polycaprolactone and chitosan were used to modify filter paper to prepare paper microarrays. Parameters of paper device preparation were optimized. Our findings suggested that 3D-printing paper-based microarrays can be a simple and low-cost approach for discovery of active compounds of TCM.
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高影响因子
Journal of the Mechanical Behavior of Biomedical Materials
·
2019
·
IF 3.485
Tao Feng,Junjie Niu,Bin Pi,Yingjie Lu,Jinning Wang,Wen Zhang,Bin Li,Huilin Yang,Xuesong Zhu
High brittleness and lack osteogenesis are two major limitations of calcium phosphate cement (CPC) in application in bone defect reconstruction. Here we prepared a composite calcium phosphate cement by mixing N-acetyl cysteine loaded silk fibroin solution with α-tricalcium phosphate. In vitro cytology experiment revealed that SF-NAC/α-TCP could significantly increase the activity of exocrine ALP and up-regulated expression of bone-related genes. However, NAC up-regulated gene expression could be significantly suppressed by DKK1. We propose that NAC functioning as osteogenic factor by activating the Wnt/β-catenin signaling pathway may be the possible mechanism of up-regulation of osteogenic genes. Bone regeneration in vivo shown in a rat femur defect was enhanced by the addition of NAC in SF/α-TCP. In addition, the combination intensity of cement-bone interface was improved. The combination SF-NAC/α-TCP might be developed into a promising tool for bone tissue repair in the clinic.
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高影响因子
JOURNAL OF FOOD BIOCHEMISTRY
·
2022
·Vol. 46
·期 10
·e14280
·
IF 3.654
Tian Liang,Jiawei Hu,He Song,Lili Xiong,Yanping Li,Yang Zhou,Lifang Mao,Jiamin Tian,Huan Yan,Ersheng Gong,Jiawen Fei,Yuan Sun,Hanyue Zhang,Xiaoyin Wang
This study aimed at comparing the physicochemical characteristics, α-glucosidase inhibitory effect, and hypoglycemic activity of pectins (N-NOP and H-NOP) from peels of normal and Huanglongbing (HLB)-infected Navel oranges. Results indicated the pectins were high methoxy pectins mainly composed of homogalacturonan and rhamnogalacturonan-I. The pectins exhibited similar functional groups, surface morphology, and particle size, and had no triple-helical conformation in solution. They exerted fat and glucose absorption capacities and were mixed-type noncompetitive α-glucosidase inhibitors with IC 50 values of 1.182 and 2.524 mg/ml, respectively. Both N-NOP and H-NOP showed hypoglycemic activity in alloxan-induced diabetic mice. Administration of them could promote the synthesis of hepatic glycogen and/or serum insulin to lower blood glucose levels and enhance antioxidant status to alleviate oxidative stress injury in diabetic mice. Moreover, N-NOP had higher yield, molecular weight, ζ-potential, oil holding capacity, α-glucosidase inhibitory effect and in vivo hypoglycemic activity, whereas H-NOP possessed higher uronic acid, degree of esterification, thermal stability, water holding capacity, swelling capacity, and fat absorption capacity. It could be concluded that some similarities and differences existed between N-NOP and H-NOP in physicochemical characteristics, functional properties, α-glucosidase inhibitory effects, and hypoglycemic activity. This study provides references for the basic research and application of pectins from peels of normal and HLB-infected Navel oranges. Practical applications Pectin has been widely used in the food and pharmaceutical industries for several decades due to its health benefit, gelling, thickening, and emulsification performances. Diabetes mellitus is a worldwide concern in recent years. Pectins (N-NOP and H-NOP) from peels of normal and Huanglongbing (HLB)-infected Navel oranges possessed in vitro and in vivo hypoglycemic activities, indicating they were potential anti-antidiabetic substitutes of chemical drugs. Moreover, comparative understanding on the physicochemical characteristic, α-glucosidase inhibitory effect and hypoglycemic activity of pectins from peels of normal and Huanglongbing-infected Navel oranges was conducive to the recycling and utilization of Navel orange peels. Recently, the biological activity of pectin from peels of normal Navel oranges has been rarely reported, and the information on pectin from peels of Huanglongbing-infected Navel orange is rare. This study provides references for the basic research and application of pectins from peels of normal and HLB-infected Navel oranges.
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高影响因子
JOURNAL OF MATERIALS ENGINEERING AND PERFORMANCE
·
2021
·Vol. 30
·期 5
·3484-3491
·
IF 1.819
Gao Yong,Zhang Haibo,Xu Zengshi
Molybdenum trioxide holds the promise of high-performance cathodes for lithium-ion batteries. This study introduces a facile one-step method for large-scale synthesizing B, C co-doped α -MoO 3 single crystals by simultaneous decomposition of boric acid, carburization and vapor deposition on a molybdenum substrate. Experimental characterizations indicate that the B and C co-doping promotes the carrier mobility by inducing impurity states near the Fermi level. Additionally, when subjected to the high voltage, the Mo-C and Mo-B bonds become the controlling factors for enhancing the structural stability through the reduced interplanar spacing which facilitates the restriction of transition-metal ion migration. The target material delivers a high discharge capacity of 305 mAh g −1 with initial Coulombic efficiency of 98%, and shows remarkable cycle stability with a specific capacity of 280 mAh g −1 after 80 cycles. This study sheds light on a new strategy for designing and controlling of the doping activity in other transition-metal-oxides for next-generation lithium-ion batteries.
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MOLECULES
·
2020
·Vol. 25
·期 19
·4507
·
IF 3.267
Zhen Li,Zongcai Tu,Hui Wang,Lu Zhang
Ceratophyllum demersumL. (CDL) is a traditional Chinese herb to treat many diseases, but research on its anti-diabetic activity is not available. In this research, the α-glucosidase inhibitory ability and phytochemical constituents of CDL extract were firstly studied. Optimal ultrasound-assisted extraction conditions for α-glucosidase inhibitors (AGIs) were optimized by single factor experiment and response surface methodology (RSM), which was confirmed as 70% methanol, liquid-to-solid ratio of 43 (mL/g), extraction time of 54 min, ultrasonic power of 350 W, and extraction temperature of 40 °C. The lowest IC50value for α-glucosidase inhibition was 0.15 mg dried material/mL (mg DM/mL), which was much lower than that of acarbose (IC50value of 0.64 mg DM/mL). In total, 80 compounds including 8 organic acids, 11 phenolic acids, 25 flavonoids, 21 fatty acids, and 15 others were identified or tentatively identified from CDL extract by HPLC-QTOF-MS/MS analysis. The results suggested that CDL could be a potential source of α-glucosidase inhibitors. It can also provide useful phytochemical information for research into other bioactivities.Keywords:Ceratophyllum demersumL.;response surface methodology;α-glucosidase inhibitors;phytochemical profile
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Catalysis Science & Technology
·
2015
·Vol. 6
·期 6
·1693-1700
·
IF 5.426
Zhixin Zhang,Yehong Wang,Min Wang,Jianmin Lu,Chaofeng Zhang,Lihua Li,Jingyang Jiang,Feng Wang
We herein report the oxidative C–C coupling of ketones and primary alcohols to produce α,β-unsaturated ketones in the absence of base additives. This cascade synthetic reaction was conducted at 150 °C in 12 h using a heterogeneous CeO2 catalyst. The conversion of acetophenone reached 74% with 89% selectivity to chalcone. A correlation between the CeO2 crystal plane and catalytic performance is established as the catalytic activities decrease in the sequence of (110) > (111) > (100). Characterization using Raman spectroscopy, CO2 temperature-programmed desorption (CO2-TPD), and in situ active site-capping tests has shown that the unusual catalysis of the CeO2 catalyst is attributed to the coexistence of basic and redox active sites. These sites synergistically catalyze the oxidation of alcohols to aldehydes and the aldol condensation to ketones. Moreover, the CeO2 catalyst can be reused several times after calcination to remove the surface-adsorbed substances.
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Materials Today Communications
·
2024
·
IF 3.7
Junliang Fan,Li Xiao,Yuantao Qin,Hao Yan,Xiaolin Qu,Zhenzhen Li,Hengxiang Gong
There is little research on α-Ga 2 O 3 single crystal film because it is a metastable phase of Ga 2 O 3 crystal and difficult for controllable fabrication. In this paper, highly crystallized Ga 2 O 3 thin films of poly-crystalline, α-Ga 2 O 3 and β-Ga 2 O 3 were prepared by modulating the growth temperature from 400 °C to 700 °C by mist-assisted CVD (Mist CVD) method. The results show that films grown at 525 °C owes the best crystalline quality of α-Ga 2 O 3 film. To further improve the crystallinity of α-Ga 2 O 3 thin film, a systematic simulation revealing the distribution changes of aerosol particle size, flow field, and temperature gradient in the reaction chamber were explored. The optimal growth region C with uniform particle size, well-distribute temperature gradient and stable flow field is determined. Meanwhile, the experimental results of α-Ga 2 O 3 thin films grown at different positions in the reaction chamber was proved to consistent with the simulation results. Furthermore, the optimal single crystallized α-Ga 2 O 3 thin films with smooth surface morphology and optical property were obtained. The PL spectrum operated at room temperature show the α-Ga 2 O 3 film with low defect density and high crystalline quality. It shows that the single crystallized α-Ga 2 O 3 is orientated grown on sapphire substrate with epitaxial relationship of α-Ga 2 O 3 (0006)//c-Al 2 O 3 (0006). Although, slight lattice mismatch is observed on the selected area electron diffraction pattern of the interface between sapphire and α-Ga 2 O 3 film, it is ideal for further application in devices. This work reveals the key point for α-Ga 2 O 3 single crystal film growth by mist CVD is to construct a steady and orderly environment through controlling aerosol particle state.
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Journal of Materials Chemistry A
·
2015
·Vol. 3
·期 46
·23607-23620
·
IF 7.443
Zhifeng Jiang,Deli Jiang,Wei Wei,Zaoxue Yan,Jimin Xie
In this work, we fabricated size-tunable metal (Pd, Ag) nanoparticles grafted on bionic dendritic α-Fe2O3 (M@Fe2O3) through an in situ growth method by using carbon nanodots as a reducing agent followed by a hydrothermal reaction. The size of the supported noble metal nanoparticles can be directly controlled on the surface of dendritic α-Fe2O3 by adjusting the added amount of noble metal salts. The catalytic performances of the resulting M@Fe2O3 hybrid composites were firstly evaluated by reduction of p-nitrophenol. The results indicated that the size of the noble metal nanoparticles had a crucial influence on the catalytic behaviors of the M@Fe2O3. In addition, the dendritic M@Fe2O3 also showed improved photocatalytic efficiencies for visible-light-driven MB degradation due to synergic effects between dendritic α-Fe2O3 and noble metal nanoparticles, including optical properties, unique structure, good dispersion, good charge separation and the SPR effect. The M@Fe2O3 composites have good reproducibility and are highly stable for p-nitrophenol reduction/degradation of MB. Our strategy can be extended to a general strategy for the formation of other novel composites consisting of a 3D hierarchical metal oxide and noble metal nanoparticles with tailorable size (e.g., Pt@TiO2, Pd@MnO2, Au@ZnO and Ag@Cu2O) for broad applications in water treatment.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Changdong Lin,Juyuan Luo,Shuo Wang,Cheng Shen,Shucan Zhang,Zhibo Song,Yumei Ma,Yongling Liu,Yulin Li,Tao Chen
Resveratrol with excellent α-glucosidase inhibitory activity is widely distributed in foods. However, in-depth research on the effects of glycosylation and acylation of resveratrol on its α-glucosidase inhibitory activity is limited. In the present study, we investigated the structure-activity relationship of resveratrol, resveratrol-4’- O -glucoside, resveratrol 4’- O -(6′′- O -galloyl)-glucoside and resveratrol 4’- O -(2′′- O -galloyl)-glucoside against α-glucosidase. Notably, C-4′ glycosylation enhanced potency, and subsequent galloyl acylation further improved activity with acylation at C-6′′ slightly outperforming that at C-2′′. Spectroscopic analyses confirmed conformational changes in the enzyme upon binding. Molecular docking showed C-4′ glycosylation led to the formation of additional hydrogen bonds with α-glucosidase, and subsequent acylation further increased their number. Molecular dynamics simulations showed resveratrol 4’- O -(6′′- O -galloyl)-glucoside, owing to its lower steric hindrance than resveratrol 4’- O -(2′′- O -galloyl)-glucoside, penetrates more deeply into the active-site pocket of α-glucosidase, resulting in a more stable binding. These findings may provide theoretical basis for the development of resveratrol and its derivatives in functional foods.
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产品关联
DOI
PubMed
高影响因子
Frontiers in Chemistry
·
2022
·
IF 5.545
Yang Shuhui,Ke Xi,Zhang Menglong,Luo Dongxiang
Metal nanoparticles have been extensively used as co-catalysts in photocatalytic systems in order to pursue improvements in both reaction kinetics and selectivity. In this work, PdAg dual-metallic nanoparticles synthesized by the co-reduction method were decorated on a well-established α-Fe2O3/CdS Z-scheme photoactive material as a co-catalyst to study their performance for promoting the photoreduction of CO2. Herein, α-Fe2O3 and CdS were in situ synthesized on fluorine-doped tin oxide (FTO) glass by hydrothermal and SILAR (successive ionic layer adsorption and reaction) methods, respectively. The direct Z-scheme charge transfer path between Fe2O3 and CdS and the effective electron migration toward the PdAg mainly contributed to the excellent photocatalytic CO2 reduction performance. The controllable work function based on Pd (5.12) and Ag (4.26) constructed an appropriate band alignment with α-Fe2O3/CdS and displayed favorable production for CH4 rather than CO. The optimum ratio of PdAg 1:2 performed a 48% enhancement than pure Pd for photoreduction of CO2. Meanwhile, the enhanced charge separation improved the photoelectrochemical performance and photocurrent generation, and reduced the electrical resistance between components. This work provided insights into the dual-metallic co-catalyst for boosting the activity and selectivity of photocatalytic CO2 reduction.
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高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
·
2019
·
IF 14.229
Siqi Huang,Qun Zhang,Pengyu Liu,Shengjia Ma,Bing Xie,Kai Yang,Yaping Zhao
In this study, novel up-converted carbon quantum dots (CQD) incorporated goethite (α-FeOOH) nanohybrids (CQDs/α-FeOOH) were fabricated via a facile hydrolysis method. CQDs/α-FeOOH exhibited remarkably degradation efficiency towards tetracycline (TC, 0.1503 min -1 ), approximately 3.7 times than pristine α-FeOOH with much lower amount of H 2 O 2 (0.5 mM) under visible light irradiation (λ＞420 nm). The quenching and electron spin resonance (ESR) tests proved · OH, O 2 · - and 1 O 2 were involved in TC degradation in CQDs/α-FeOOH catalyzed Fenton system. The potential Ultraviolet-Visible-Infrared (UV–vis-IR) broad-spectrum responsive properties of CQDs/α-FeOOH were proved in degradation various surface charged organic contaminants regarding the function of coupling up-conversion CQDs. CQDs/α-FeOOH also exhibited wide application pH, excellent durability, fairly good catalytic performance within different practical water matrices and completely eliminated related drug resistance of TC even under real solar-light conditions. The good biocompatibility makes CQDs/α-FeOOH served as a promising photocatalyst for remediation of water contamination with efficient utilization of solar energy.
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GREEN CHEMISTRY
·
2017
·Vol. 19
·期 20
·4838-4848
·
IF 9.125
Gang Wang,Yiming Xu,Suojiang Zhang,Zengxi Li,Chunshan Li
A one-pot synthesis of α,β-unsaturated esters from unactivated esters and aldehydes using strong bases, such as sodium alkoxide and potassium tert-butoxide, was reported. However, the ionic liquid (IL) catalyzed probase method for producing α,β-unsaturated esters was not reported until now. In this work, a series of ILs with fluoride anions were firstly prepared and used as catalysts in combination with the probase N,O-bis(trimethylsilyl) acetamide (BSA) for the α,β-unsaturated esters synthesis. This process could also be promoted through the introduction of another IL with Lewis acid sites. The yield and selectivity of the product could reach up to 84.2% and 95.0%, respectively, when [Bmim]F was used in combination with [Bmim]Cl/AlCl3 (the molar fraction of AlCl3 is 0.67). The mechanism investigation through GC-MS indicates that BSA would convert into onium amide, which acted as a strong base for α-H abstraction, with the catalysis of [Bmim]F. Meanwhile, [Bmim]Cl/AlCl3 played an important role in the condensation step between enolates and aldehydes. On the basis of mechanism insights, kinetic and thermodynamic studies were also carried out for a better understanding of this new route.
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