ACS Applied Nano Materials
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2022
·
IF 6.14
Jiandan Chen,Guangxian Liu,Qi Xiao,Chenwei Wang,Zhenzeng Zhou,Chunchuan Gu,Zheng-Zhi Yin,Hongying Liu
Glucose concentration in the body is a very important index for health evaluation. Nonenzymatic glucose biosensors attract persistent interest and research. The acupuncture technique has been developed for treating the disease for thousands of years. Here, a stainless-steel acupuncture needle (AN) was first used to sense glucose after functionalizing with bimetal of gold nanoparticles (AuNPs) and nickel nanoparticles (NiNPs). AuNPs were electrodeposited as particle clusters, and the NiNPs could be further electrodeposited around AuNPs to form a nano-pinecone shape with a size of about 600 nm. Au/AN exhibited the best electroconductivity compared to the weakest Ni/AN and medium Ni/Au/AN interfaces. Three nanotechnically processed interfaces showed obvious electrochemical response behavior for glucose compared with the bare AN. Surprisingly, the matrix of AuNPs could drastically boost the electrochemical response of NiNPs for glucose, which might be due to the abundance of active sites in the bimetallic hierarchy. The Ni/Au/AN electrode possessed an additive signal compared with Au/AN and Ni/AN. Under the optimized conditions, the prepared nonenzymatic biosensor exhibited two linear relationships in the wide concentration range of 0.5 μM to 1.5 mM and 1.5–5 mM with sensitivities of 766.02 and 368.77 (μA/mM/cm2), and the limit of detection was 0.14 μM. Moreover, the Ni/Au/AN showed superior performance over reported electrodes with great selectivity, stability, and reproducibility. The apparent Km of 0.161 mM was lower than that of glucose oxidase, implying that the Ni/Au-modified AN exhibited a strong affinity for glucose. The fabricated biosensor can be successfully used for the detection of glucose concentration in human serum, which provided a leading direction and potential for clinical point-of-care monitoring.
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FOOD CHEMISTRY
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2022
·
IF 9.231
Xiudan Wang,Yilin Wang,Chen Guo,Xiaoyun Zhang,Ying Wang,Liangtao Lv,Xiaokun Wang,Manman Wei
In this study, a paper-based enzyme biosensor for hypoxanthine (Hx) was developed, enabling visual and one-step fish freshness detection. Xanthine oxidase and horseradish peroxidase were immobilized on nitrocellulose membranes with 3,3′,5,5′-tetramethylbenzidine to output the colour signal. Chitosan oligosaccharide lactate-modified nitrocellulose membranes entrapped the dual-enzyme system and exhibited excellent microfluidic aggregation effect. The developed enzyme biosensor produced a linear response of 0.01–0.16 mmolL -1 with a detection limit of 8.22 μmolL -1 , and was selective for Hx with recoveries of 96.13–103.11 % for fish samples. These biosensors were attached directly to the surface of fish samples and the colour was revealed within 3 min. Colour signals can be judged by the naked-eye to distinguish between fresh and spoiled fish samples and analyzed by a smartphone for quantitative analysis. The biosensor shows great potential as a powerful pattern- and reagent-free device for on-site freshness evaluation of fish.
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PubMed
高影响因子
TALANTA
·
2022
·
IF 6.556
Pengfei Zhao,Sisi Tang,Xiaodan Wu,Yu Chen,Kangling Tang,Jinli Fu,Huibin Lei,ZhaoXia Yang,Zhaohui Zhang
Development of method with micro-volume reagent usage and high selectivity for rapid determination of valuable disease-related biomarkers is of great importance for disease surveillance. Herein, a novel ratiometric fluorescence capillary imprinted sensor was developed for highly selective fluorescence determination of sialic acid (SA) based on luminescent metal-organic framework (UiO-66-NH 2 ). In this strategy, the imprinted pre-polymer with double emissions was sucked into the capillary to grow imprinted layer on the inner wall of the capillary. In presence of SA, the blue fluorescence (440 nm) served as fluorescence response signal was quenched due to the electron transfer mechanism between metal organic framework and SA while the red fluorescence (655 nm) served as the reference signal was remained. Within SA concentration linear range of 1.0 × 10 −7 –3.0 × 10 −4 M, the ratiometric fluorescence capillary imprinted sensor was applied to detect SA in human serum and saliva samples successfully with a detection limit of 2.5 × 10 −8 M. A sensitive and selective fluorescence method with micro-volume sample consumption (15 μl) for determination of SA in practical samples was established.
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SENSORS AND ACTUATORS B-CHEMICAL
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2022
·
IF 9.221
Ming Shang,Haiyan Wei,Ge Gao,Nianlu Li,Wei Zou,Ranran Liu,Mengping Zhang,Xiao Meng,Wenwen Chen,Yaxin Sun,Cuijuan Wang
Bromadiolone is the most frequently detected anticoagulant rodenticide in human biofluids, resulting in increased cases of poisoning. Here, we present a portable kit that could realize rapid fingerprint detection of DQ in human biofluids. This strategy was based on surface-enhanced Raman scattering (SERS) and salt-induced liquid-liquid phase separation (SILLPS) using a gold/silver core-shell nanoparticle (Au@Ag NPs) substrate. The kit included a portable Raman spectrometer and five tubes that contained Au@Ag NPs colloid, KI solution, saturated NaCl solution, acetonitrile , and Mg(NO 3 ) 2 solution. This strategy did not require pretreatment for urine samples. For blood samples, a 2-min SILLPS method was proposed with satisfactory recovery, providing unique insights into efficient pretreatment of complex biomatrix. The detection limits for urine and blood were calculated to be 3.21 ppb and 6.86 ppb, respectively. A clear trend could be observed between signal intensity and logarithmic concentration of bromadiolone ranging from 0 to 2 ppm with high reproducibility. Such a portable kit based on SILLPS-SERS can rapidly provide fingerprint information of poison. This simple-yet-practical “Pretreat & Detect” SERS kit may open up new opportunities for point-of-care detection of poisons and has great potential as a tool in field applications.
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Journal of Analysis and Testing
·
2022
·Vol. 6
·期 4
·382-392
·
IF 0
Bai Li-Wei,Shi Yu-Fen,Zhang Xue,Liu Xue-Bo,Wu Feng,Liu Chang,Jia Jian-Feng,Lu Wen-Bo
Hydroquinone (HQ) poses immeasurable risk to human health and the natural environment on the grounds of high toxicity of organic phenolic compounds. Herein, an innovative electrochemical sensor based on two-dimensional nickel molybdate nanowires (NiMoO 4 NWs) is constructed for the ultra-sensitive determination of HQ, which can provide useful reference for the human health and environment protection. Two-dimensional NiMoO 4 NWs are prepared successfully through a facile hydrothermal reaction and annealing process. The obtained two-dimensional NiMoO 4 NWs are characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), high-resolution TEM (HRTEM), energy-dispersive X-ray spectroscopy (EDS) mapping and X-ray photoelectron spectroscopy (XPS). The accurate contents of Ni and Mo have been characterized by inductively coupled plasma atomic emission spectroscopy (ICP-AES), confirming that the lower content of the nickel in NiMoO 4 NWs possesses the higher catalytic activity of HQ. Under the optimized conditions, the constructed HQ sensor exhibits satisfactory electrocatalytic activity with a low detection limit of 0.0355 μmol/L ( S/N = 3), a wide linear range of 0.05–4600 μmol/L and sensitivity of 170.064 μA/(mmol cm 2 ). The sensor has been successfully applied to the detection of HQ in rainwater, tap water, domestic sewage and drinking water samples with satisfactory recovery. At the same time, this sensor has excellent reproducibility, selectivity, and stability. The constructed sensor has potential practical application value and broad application prospect in human health and environmental monitoring.
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CELLULOSE
·
2022
·Vol. 29
·期 16
·8919-8935
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IF 6.123
Fan Shuang,Chang Wei,Fei Cheng,Zhang Zhongguo,Hou Bingbing,Shi Zhuxuan,Wang Huixin,Hui Yuchen
A wearable self-powered biosensor, fabricated from a stretchable and bendable textile matrix, senses glucose in urine. This textile matrix is made of cellulose fibers. A porous three-dimensional electrode with high flexibility, conductivity, and electroactive surface area is obtained by coating multi-wall carbon nanotubes and reduced graphene oxides onto a textile matrix based on cellulose fibers. After immobilizing glucose oxidase and laccase by the polymer embed method, the bioelectrodes show good enzymatic loading density and high electrocatalytic efficiency. The relative self-powered biosensor has a good linear response to glucose in the concentration range from 0 to 40 mM (R 2 = 0.995), with a satisfactory limit of detection at 3.95 mM, owing to the high electrocatalytic efficiency of the bioelectrodes. The biosensors have good selectivity, repeatability, and long-term storage stability, exhibit excellent stretching and bending performance, and show stability to a variety of pH, because of the catalytic specificity and tolerance of enzymes, the reasonable design of the biosensor, and the stable catalytic microenvironment. The recovery of the biosensor integrated with a diaper for glucose in artificial urine samples is between 95.6 and 105.9%, suggesting a promising determination for real samples. Graphical abstract
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Pharmaceutics
·
2022
·Vol. 14
·期 11
·2307
·
IF 6.525
Xueyan Gou,Fenglin Ran,Jinru Yang,Yanrong Ma,Xin’an Wu
Background: Organic anion transporter 1 (OAT1) and OAT3 have an overlapping spectrum of substrates such that one can exert a compensatory effect when the other is dysfunctional. As a result, the knockout of either OAT1 or OAT3 is not reflected in a change in the excretion of organic anionic substrates. To date, only the mOAT1 and mOAT3 individual knockout mouse models have been available. Methods: In this study, we successfully generated aSlc22a6/Slc22a8double-knockout (KO) rat model using CRISPR/Cas9 technology and evaluated its biological properties. Results: The double-knockout rat model did not expressionmRNAfor rOAT1 or rOAT3 in the kidneys. Consistently, the renal excretion ofp-aminohippuric acid (PAH), the classical substrate of OAT1/OAT3, was substantially decreased in theSlc22a6/Slc22a8double-knockout rats. The relativemRNAlevel ofSlco4c1was up-regulated in KO rats. No renal pathological phenotype was evident. The renal elimination of the organic anionic drug furosemide was nearly abolished in theSlc22a6/Slc22a8knockout rats, but elimination of the organic cationic drug metformin was hardly affected. Conclusions: These results demonstrate that this rat model is a useful tool for investigating the functions of OAT1/OAT3 in metabolic diseases, drug metabolism and pharmacokinetics, and OATs-mediated drug interactions.Keywords:OAT1/OAT3;CRISPR/Cas9;gene knockout;pharmacokinetic
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高影响因子
Advanced Materials Interfaces
·
2022
·Vol. 10
·期 1
·2200738
·
IF 6.389
Bo Wu,Minzhang Li,Rajendran Ramachandran,Gaoqiang Niu,Mingxiang Zhang,Changhui Zhao,Zongxiang Xu,Fei Wang
Dopamine (DA) and uric acid (UA) are biomolecules of great consideration that coexist in human body. It is essential and challenging to detect them simultaneously with high sensitivity. This paper reports a novel electrochemical biosensor based on the nanocomposites of cobalt phthalocyanine (CoPc) anchored with graphene quantum dots (GQDs) for DA and UA detection. The GQDs incorporation on CoPc nanorods with partial end stretching significantly enhances the electrocatalytic activity and promotes the electron transfer rate. Low detection limits of 21 n m for DA and 145 n m for UA have been successfully achieved for the fabricated CoPc/GQDs biosensor. The density-functional theory proves the enhanced conductivity of CoPc/GQDs and the biomolecule's interaction with the electrode surface. Furthermore, the proposed sensor shows remarkable reproducibility, repeatability, and stability. The fabricated sensor is applied for the simultaneous detections of DA and UA in human urine samples. The excellent performance suggests that the CoPc/GQDs could be a promising and potential candidate for electrochemical sensors to detect DA and UA selectively.
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Journal of Materials Research and Technology-JMR&T
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2022
·
IF 6.267
Tongle Gao,Ting-Ting Li,Xilin Liao,Jia-Horng Lin,Bing-Chiuan Shiu,Ching-Wen Lou
Photoelectrodes constructed with photoactive materials are of great significance for the performance of photoelectrochemical (PEC) sensors. In this study, a facile and efficacious non-enzymatic glucose sensor based on the Cu 2 O/TiO 2 heterojunction was successfully constructed for quantitative determination of glucose concentration. The Cu 2 O/TiO 2 heterojunction was generated by first obtaining TiO 2 nanofibers via electrostatic spinning and high-temperature calcination, and then synthesizing Cu 2 O nanoparticles via a one-step hydrothermal reaction. By introducing the narrow band gap semiconductor Cu 2 O, the defect that pure TiO 2 could not absorb visible light was improved, resulting in the improved photoelectrochemical performance of the composite material compared to the single component semiconductor. The sensor exhibited favorable PEC non-enzymatic glucose sensing performance under simulated sunlight irradiation with a wide linear response range from 1 mM to 14 mM (R 2 = 0.9991) and a low detection limit of 14.4 μM (S/N = 3), along with good selectivity, stability and reproducibility. This also provided more ideas and possibilities for preparing photoelectrode materials by constructing heterojunctions.
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MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 10
·1-13
·
IF 6.408
Wang Fang,Shi Fengna,Chen Cheng,Huang Kexin,Chen Naipin,Xu Ziqi
Cobalt hydroxide nanoparticles (Co(OH) 2 NPs) were uniformly deposited on flexible carbon cloth substrate (Co(OH) 2 @CC) rapidly by a facile one-step electrodeposition, which can act as an enzyme-free glucose and uric acid sensor in an alkaline electrolyte. Compositional and morphological characterization were examined by X-ray diffraction (XRD), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDS), which confirmed the deposited nanospheres were Co(OH) 2 nanoparticles (NPs). The electrochemical oxidation of glucose and uric acid at Co(OH) 2 @CC electrode was investigated by electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), differential pulse voltammetry (DPV), and chronoamperometry methods. The results revealed a remarkable electrocatalytic activity toward the single and simultaneous determination of glucose and uric acid at about 0.6 V and 0.3 V (vs. Ag/AgCl), respectively, which is attributed to a noticeable synergy effect between Co(OH) 2 NPs and CC with good repeatability, satisfactory reproducibility, considerable long-term stability, superior selectivity, outstanding sensitivity, and wide linear detection range from 1 uM to 2 mM and 25 nM to 1.5 uM for glucose and UA, respectively. The detection limits were 0.36 nM for UA and 0.24 μM for glucose (S/N = 3). Finally, the Co(OH) 2 @CC electrode was utilized for glucose and uric acid determination in human blood samples and satisfying results were obtained. The relative standard derivations (RSDs) for glucose and UA were in the range 6 to 14% and 0 to 3%, respectively. The recovery ranges for glucose an UA were 97 to 103% and 95 and 101%, respectively. These features make the novel Co(OH) 2 @CC sensor developed by a low-cost, efficient, and eco-friendly preparation method a potentially practical candidate for application to biosensors. Graphical abstract
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高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
·
2022
·
IF 3.705
Ran Bi,Xiaoyan Ma,Kunpeng Miao,Pengcheng Ma,Qianqian Wang
With the development of blood glucose monitoring medical devices, higher requirements are put forward for the sensitivity and detection limit of biosensors. Herein, a high-performance flexible enzymatic glucose biosensor was prepared by electrochemical deposition of dendritic gold nanostructure on carbon cloth substrate, then covalently immobilizing glucose oxidase on the dendritic gold, and then increasing enzyme loading by enzyme precipitation coating. The morphology of dendritic gold nanostructures was characterized by scanning electron microscopy (SEM). The electrochemical performance of the biosensor was analyzed by cyclic voltammetry (CV), chronoamperometry curve and electrochemical impedance spectroscopy (EIS). It is found that the obtained biosensor has high sensitivity (72.45 μA mM −1 cm −2 ), low detection limit (6.7 μM) and ultra-wide linear range (0.02–31.7 mM) for glucose detection. In addition, the biosensor also has good selectivity, reproducibility and stability. In the detection of real serum samples, the biosensor also shows good accuracy and practicality. The results show that the new biosensor has great potential for applications in biomedical and health care.
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Food Science and Human Wellness
·
2022
·
IF 8.022
Hongyuan Zhao,Xiaoyu Chen,Fanqiang Meng,Libang Zhou,Xinyi Pang,Zhaoxin Lu,Yingjian Lu
Hyperuricemia is a critical threat to human health, and a high inosine diet can increase the prevalence of it. Lacticaseibacillus rhamnosus Fmb14 was isolated from traditional fermented Chinese yogurt, and its inosine degradation rate reached 36.3 % at 10 9 CFU/mL for 24 h. LC-MS analysis revealed that high concentrations of inosine could activate compensatory metabolic pathways of L. rhamnosus Fmb14 to catalyse inosine as an energy source and produce intracellular folic acid and riboflavin. The contents of folic acid and riboflavin were 6.0 and 4.3 fold increased after inosine treatment in the cell-free extracts (CFE). L. rhamnosus Fmb14 CFE treatment ameliorates hyperuricemia through xanthine oxidase (XOD) inhibition and ATP-binding cassette subfamily G member 2 (ABCG2) promotion, both of which are responsible for uric acid (UA) synthesis and secretion in HepG2 and Caco2 cells, respectively. The in vivo results showed that the serum UA level decreased from 236.28 to 149.28 μmol/L after 8 weeks of oral administration of L. rhamnosus Fmb14 in inosine-induced hyperuricemia model mice. Our results revealed that L. rhamnosus Fmb14 has a potential as a biological therapeutic agent in hyperuricemia prevention.
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