Journal of Pharmaceutical Analysis
·
2019
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IF 4.44
Ningning Zhao,Shu Liu,Junpeng Xing,Zifeng Pi,Fengrui Song,Zhiqiang Liu
Enrichment of trace bioactive constituents and metabolites from complex biological samples is challenging. This study presented a one-pot synthesis of magnetic polydopamine nanoparticles (Fe 3 O 4 @SiO 2 @PDA NPs) with multiple recognition sites for the magnetic dispersive solid-phase extraction (MDSPE) of ginsenosides from rat plasma treated with white ginseng . The extracted ginsenosides were characterized by combining an ultra-high-performance liquid chromatography coupled to a high-resolution mass spectrometry with supplemental UNIFI libraries. Response surface methodology was statistically used to optimize the extraction procedure of the ginsenosides. The reusability of Fe 3 O 4 @SiO 2 @PDA NPs was also examined and the results showed that the recovery rate exceeded 80% after recycling 6 times. Furthermore, the proposed method showed greater enrichment efficiency and could rapidly determine and characterize 23 ginsenoside prototypes and metabolites from plasma. In comparison, conventional methanol method can only detect 8 ginsenosides from the same plasma samples. The proposed approach can provide methodological reference for the trace determination and characterization of different bioactive ingredients and metabolites of traditional Chinese medicines and food.
研究领域:
分析化学
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中药学
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生物医学
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DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2018
·Vol. 410
·期 7
·1885-1891
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IF 3.307
Su Yu,Shao Chenggang,Huang Xielin,Qi Jinxia,Ge Renshan,Guan Huaqin,Lin Zhenkun
A new type of magnetic nanoparticles (MNPs), as the absorbents of bisphenol A (BPA), was prepared by functionalization of Fe 3 O 4 @SiO 2 with BPA-specific aptamer in this work. ssDNA aptamer was immobilized on the Fe 3 O 4 @SiO 2 surface through biotin-avidin interactions, playing a role of the specific probe for BPA. The resultant materials (Apt-MNPs) exhibited outstanding magnetic responsibility and can be separated efficiently by the magnetic field. Experimental results also showed that Apt-MNPs had large adsorption capacity and high competitive selectivity for the targeted compound BPA. Furthermore, Apt-MNPs were adopted as the specific absorbents to extract and enrich BPA from human serum and urine samples. Therefore, an efficient detection method of BPA was developed in combination with high-performance liquid chromatography (HPLC). The linearity of the method was over a range of 5–10,000 ng mL −1 with a correlation coefficient of 0.99997, and the limit of detections (LODs) for serum and urine were 2.0 and 1.0 ng mL −1 , respectively. The recoveries of BPA in the spiked human serum and urine samples were 90.8 ± 7.3% (RSD) and 92.3 ± 1.5%, respectively. Our results demonstrated that Apt-MNPs were high-performance adsorbents for extracting and enriching BPA, resulting in fast and efficient detection of BPA in serum and urine samples.
Aptamer-MNPs were effective for BPA separation from serum and urine
研究领域:
分析化学
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环境科学
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材料科学
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生物技术
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DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Qiao Yan,Pingping Zhang,Menglin Wang,Ling Ma,Ke Wang,Kaoqi Lian
This study aimed to synthesize a novel magnetic covalent organic framework (COF)-based material and establish an efficient magnetic solid-phase extraction (MSPE) method for the determination of benzimidazoles (BZDs) in honey. Given the abundant nitrogen atoms in BZDs, the target material (Fe₃O₄@SiO₂@MPA-TAPT-COF) was designed with Fe₃O₄ as the magnetic core and a COF shell polymerized from 5-bromobenzene-1,3-dicarbaldehyde (MPA) and 4,4',4''-(1,3,5-triazine-2,4,6-triyl) trianiline (TAPT). The synthesized Fe₃O₄@SiO₂@MPA-TAPT-COF exhibited considerable specific surface area, good magnetic responsiveness, and excellent reusability. An MSPE method using Fe₃O₄@SiO₂@MPA-TAPT-COF as the adsorbent was developed for the purification of BZDs in honey, followed by quantification via ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS). This method featured simple operation, high sensitivity, and good efficiency. Under optimized MSPE conditions, It showed good linearity for 17 BZDs in the concentration range of 0.2–100 μg/L, with correlation coefficients (R²) ≥ 0.9991. The limits of detection (LODs) for BZDs in honey were as low as 0.009–0.342 μg/kg. For spiked honey samples, the recoveries of the 17 target BZDs ranged from 61.44 % to 118.90 %.These results confirm that Fe₃O₄@SiO₂@MPA-TAPT-COF is a promising adsorbent for sample pretreatment, and the developed method provides a reliable tool for the sensitive and efficient analysis of BZDs in honey.
研究领域:
分析化学
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食品安全
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环境科学
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材料科学
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产品关联
DOI
高影响因子
Surfaces and Interfaces
·
2026
·
IF 6
Hui Zhou,Bingxin Zhu,Xiang Li,Baolei Liu,Zhigang Liu,Shihua Yu
To recover the high value-added pharmaceutical intermediate tenofovir from wastewater efficiently and conveniently, a tailored magnetic composites of Fe 3 O 4 @SiO 2 @La(OH) 3 have been prepared via solvothermal and in-situ reduction methods. It exhibits the ability to remove tenofovir with high efficiency and easily separated from matrix. Batch sorption experiments demonstrated that Fe 3 O 4 @SiO 2 @La(OH) 3 can remove tenofovir with the maximum adsorption capacity of 64.53 mg/g, the largest removal of 93.07%, and fast equilibrium time of 5 min. The kinetics of tenofovir adsorption on the magnetic composite is in line with the pseudo-second-order model, while the isotherm is fitted well by the Langmuir model, and thermodynamic parameters represent that adsorption process is spontaneous and endothermic. Moreover, the saturated Fe 3 O 4 @SiO 2 @La(OH) 3 have been desorbed by mechanical stirring with the high desorption rate of 93.02%, and the adsorption capacity maintaining 47.72 mg/g after five cycles. Importantly, the composites can be used for tenofovir removal from crude extracts of pharmaceutical wastewater, with an adsorption capacity of 51.61mg/g in 5 min. Finally, the adsorption mechanisms are explored by pH experimental evidence and XPS analysis, which show that mainly involve electrostatic attraction and the inner-sphere complexation between La(OH) 3 and phosphate groups of tenofovir via ligand exchange. All the results prove that the proposed tenofovir recovery technique, providing new ideas for the recovery of high value-added substances and waste resources rationalization of the application of development. A magnetic Fe 3 O 4 @SiO 2 @La(OH) 3 was synthesized and realized the recovery of tenofovir with high economic value in pharmaceutical wastewater crude extracts. Download: Download high-res image (249KB) Download: Download full-size image
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DOI
高影响因子
ACS Omega
·
2025
·
IF 4.3
Yoshiaki Nomura,Manabu Kiguchi,Zhongoing Liang,Mei Ma,Akira Fujishima,Nobuhiro Hanada
The triazine herbicides simetryn and prometryn may cause damage to nontarget crops due to persistent contamination of agricultural water. Photocatalysis is a promising technology for the degradation of agricultural pollutants. This study provides the first systematic integration of photocatalytic herbicide degradation with live plant bioassay validation using cost-effective Fe3O4/SiO2/TiO2 magnetic photocatalysts synthesized by a simple coprecipitation method suitable for large-scale production. The phytotoxicity of herbicide-containing water was evaluated using pak choi, a crop sensitive to triazines. Both herbicides were completely decomposed in water within 240 min after UV irradiation, and their phytotoxicity also disappeared. In the plant toxicity evaluation, prometryn exhibited temporary high toxicity exceeding that of the untreated 10 ppm solution during the photodegradation process. In previous photocatalyst studies, evaluations relied on either chemical analysis or biological tests. In this study, we developed a new rapid toxicity screening technique using plant seedlings and established a safety evaluation protocol integrating chemical analysis and plant toxicity tests. This method represents the first approach to comprehensively evaluate the safety of agricultural water.
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF RARE EARTHS
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2024
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IF 5.2
Tianhao Xia,Yuwen Yuan,Yunpeng Wang,Pragati Awasthi,Wenkun Dong,Xvsheng Qiao,Dong Chen,Shisheng Ling,Xianping Fan
This study aims to advance the development of magnetic fluorescent polymer microspheres for biomedical detection applications. Conventionally, dopants have utilized europium(III) (Eu(III)) organic complexes due to their high compatibility with polymers and strong fluorescence. However, as the common magnetic material Fe 3 O 4 can quench their fluorescence, it is hard to synthesize Eu complex-doped magnetic fluorescent materials. To maintain fluorescence in the presence of magnetic parts, in this work, we synthesized Eu-doped magnetic microspheres with multi-layered structure. Firstly, poly-(glycidyl methacrylate) (PGMA) microspheres were prepared as templates and subsequently coated with layers of Fe 3 O 4 and SiO 2 . Then, the synthesized Eu(TTA) 3 (TPPO) 2 were added into PGMA@Fe 3 O 4 @SiO 2 microspheres in either basic or acidic conditions, and covered them with an extra sol–gel layer of silica at the same time. The microspheres exhibit a core–shell structure with sub-micron dimensions (580 nm) and possess favorable superparamagnetic properties ( M s = 22.02 A·m 2 /kg, M r = 1.37 A·m 2 /kg, H c = 0.242 A/m). But the fluorescence of Eu 3+ are significantly quenched by Fe 3 O 4 , O–H oscillators, and N–H oscillators. Finally, to exclude the quenching mentioned above, the first pure SiO 2 shielding layer and the second Eu(TTA) 3 (TPPO) 2 -dispersed SiO 2 layer were coated onto PGMA@Fe 3 O 4 microspheres to prevent the energy transfer due to the quenching centers and hold the fluorescence of Eu 3+ . These findings underscore the considerable potential of these microspheres exhibiting rapid magnetic separation and stable fluorescence for bioimaging and biosensing applications.
研究领域:
材料科学
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生物医学工程
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分析化学
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产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2022
·
IF 5.518
Ben Chen,Tingting Lin,Haibo You,Liqun Fang,Chu Chu,Jintao Yang,Shengqiang Tong
The surface of SiO 2 coated magnetic nanoparticles (NMPs) was modified by bonding with sulfobutylether- β -cyclodextrin (SBE- β -CD) via carbodiimide activation for the first time. The magnetic nanoparticles were synthesized by chemical coprecipitation method under alkaline condition, and the magnetic particles were modified by citric acid . Then the surface of obtained magnetic nanoparticles was coated with SiO 2, and SBE- β -CD modified by citric acid was bonded to the surface of Fe 3 O 4 /SiO 2 MNPs under carbodiimide activation method. The synthesized functionalized magnetic nanoparticles were characterized by X-ray Diffraction, Fourier Transform Infra-Red spectroscopy, thermogravimetric analyses and transmission electron microscopy . These functional nano-sized particles were scrutinized for enantioselective liquid-liquid extraction of five racemic drugs/intermediates, including trans -paroxol, N-methyl duloxetine , fluoxetine , tropic acid and acetyltropic acid, and it was found that compared with traditional SBE- β -CD, the present magnetic silica nanoparticles bonded with SBE- β -CD showed higher enantiorecognition with some racemates using enantioselective liquid-liquid extractions. To our knowledge, this is the first-time report on preparation and application of magnetic nanoparticles bonded with SBE- β -CD in enantioselective liquid-liquid extraction for chiral separations .
研究领域:
纳米材料
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分析化学
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手性分离
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磁性材料
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超分子化学
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DOI
高影响因子
APPLIED ORGANOMETALLIC CHEMISTRY
·
2020
·Vol. 34
·期 8
·e5707
·
IF 3.14
Laiyun Zhou,Mingliang Wang,Kaixuan Wang,Tiantian Wen,Lanzhi Wang
Fe 3 O 4 @SiO 2 -CeCl 3 catalyzed chemoselective synthesis of functionalized 3-substituted-1,5-benzodiazepines via one-pot multicomponent and domino reactions has been developed. During the one-pot synthesis process, one new cycle and four new bonds (one C–C, two C–N and one C=C) were constructed by the nucleophilic addition and eliminate reaction (dehydration etc.) process, intramolecular proton transfer and cyclization process. The major advantages of the present method are good to excellent yields, shorter reaction time, simple experimental procedure, easy work up, mild reaction conditions, recyclability of the catalyst and ability to tolerate a variety of functional groups which gives economical as well as ecological rewards.
研究领域:
有机化学
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药物化学
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DOI
高影响因子
CHEMOSPHERE
·
2021
·
IF 7.086
Zhihui Chen,Jun Yao,Bo Ma,Bang Liu,Jonghyok Kim,Hao Li,Xiaozhe Zhu,Chenchen Zhao,Meseret Amde
The presence of chlorophenols in water and wastewater is considered a serious environmental issue . To eliminate these micropollutants, biodegradation of chlorophenols using enzyme-nanoparticle conjugated biocatalyst, is proposed as an economical and eco-friendly method. Herein, amino-functionalized superparamagnetic Fe 3 O 4 @SiO 2 –NH 2 nanoparticles with core-shell structure were constructed as a promising carrier for immobilization of laccase from Trametes versicolor . Compared with free laccase, Fe 3 O 4 @SiO 2 –NH 2 -Laccase displayed remarkable outcomes in all major areas such as temperature and storage stabilities, and tolerance to organic solvents and metal ions . The biocatalytic performance and reusability of Fe 3 O 4 @SiO 2 –NH 2 -Laccase were evaluated for the degradation of 2,4-dichlorophenol (2,4-DCP) and 2,4,6-trichlorophenol (2,4,6-TCP) in repeated cycles. Even after 10 successive reuses, the degradation rate of 2,4-DCP and 2,4,6-TCP were found to be 54.9% and 68.7%, respectively. The influences of solution pH, initial chlorophenol concentration, and temperature on the degradation rate of these two chlorophenols were evaluated. The degradation intermediate products including dimers, trimers , and tetramers of 2,4-DCP and 2,4,6-TCP were identified. Release of chloride ions was observed during the enzymatic degradation of these two chlorophenols. Based on the determination of intermediate products and released chloride ions, the degradation pathway that was involved in dehydrogenation , reactive radical intermediates formation, dechlorination , self-coupling and oligomers/polymers formation was proposed. The toxicity of these two chlorophenols and their intermediates was substantially reduced during the enzymatic degradation. The results of this study might present an alternative clean biotechnology for the remediation of 2,4-DCP and 2,4,6-TCP contaminated water matrices.
研究领域:
环境科学
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生物技术
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Jia Ma,Jiang-Hua Zhang,Hua-Wei Zhang,Qian-Qian Du,Zi-Xuan Li,Zhi-Fei Yang,Sha-Sha Yang,Da-Yong Zhou
It is of great importance to develop a most efficient, recyclable, and ecofriendly process to produce novel structured phospholipid emulsifiers. Herein, innovative medium-chain structured phospholipid (MCSPL) emulsifiers were synthesized through transesterification of soybean lecithins with medium-chain fatty acids (MCFAs) promoted by Zn- or Al-incorporated Fe 3 O 4 @SiO 2 , denoted by Fe 3 O 4 @SiO 2 /M (M = Zn or Al). Resultingly, Fe 3 O 4 @SiO 2 /M (M = Zn or Al) exhibited the most superior reactivity with 97.1% or 88.7% MCFA incorporation to other benchmark catalysts and also had excellent magnetic separability and recyclability. Noticeably, targeted MCSPLs possessed almost more superior emulsifying properties to other phospholipid emulsifiers, and had potential for use as oil–in–water emulsifiers. Conclusively, the present findings demonstrate that transesterification promoted by Fe 3 O 4 @SiO 2 /M (M = Zn or Al) can be a promising approach for green, economic, and highly effective synthesis of novel dual-function phospholipid emulsifiers with bioactive and emulsifying properties in food, pharmaceutical, and cosmetic industries.
研究领域:
食品科学
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化学工程
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生物技术
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2023
·
IF 5.2
Siqin He,Bin Xu,Ying Liu,Xiaoshuang Yin,Wenzhong Yang,Yun Chen
Polyaminoamide (PAMAM) dendrimers with different generations from 1 G to 5 G were grafted on magnetic core-shell Fe 3 O 4 -SiO 2 composite particles , respectively. Using Fe 3 O 4 as a magnetic center and SiO 2 as the connecting layer, the PAMAM dendrimers with amino functional groups were modified on SiO 2 successfully, while laccase was immobilized to the surface of Fe 3 O 4 -SiO 2 -PAMAM x by covalent cross-linking using amide bond. Under the optimal conditions, the loading capacity of laccase reached 65.5 mg/g, and about 62.8 % of its initial activity of free laccase was reserved. Immobilized laccase degradated 2,4-dichlorophenol (2,4-DCP) with a degradation ratio of 91.5 % after 24 h. The degradation ratio of 82.9 % was maintained after 5 cycles of the operation, and when enhanced to the 10 cycles degradation, the degradation rate was still remained close to 80 %. Compared with free laccase, the comprehensive stability of immobilized laccase was improved significantly, and it displayed the excellent catalytic degradation ability for 2,4-DCP.
研究领域:
环境科学
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生物技术
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2013
·
IF 5.008
Miaorong Pan,Yangfei Sun,Jin Zheng,Wuli Yang
In this work, core–shell–shell-structured boronic acid-functionalized magnetic composite microspheres Fe3O4@SiO2@poly (methyl methacrylate-co-4-vinylphenylbornoic acid) (Fe3O4@SiO2@P(MMA-co-VPBA)) with a uniform size and fine morphology were synthesized. Here, Fe3O4 magnetic particles were prepared by a solvothermal reaction, whereas the Fe3O4@SiO2 microspheres with a core–shell structure were obtained by a sol–gel process. 3-(Trimethoxysilyl) propyl methacrylate (MPS)-modified Fe3O4@SiO2 was used as the seed in the emulsion polymerization of MMA and VPBA to form the core–shell–shell-structured magnetic composite microspheres. As the boronic acid groups on the surface of Fe3O4@SiO2@P(MMA-co-VPBA) could form tight yet reversible covalent bonds with the cis-1,2-diols groups of glycoproteins, the magnetic composite microspheres were applied to enrich a standard glycoprotein, horseradish peroxidase (HRP), and the results demonstrated that the composite microspheres have a higher affinity for the glycoproteins in the presence of the nonglycoprotein bovine serum albumin (BSA) over HRP. Additionally, different monomer mole ratios of MMA/VPBA were studied, and the results implied that using MMA as the major monomer could reduce the amount of VPBA with a similar glycoprotein enrichment efficiency but a lower cost.
研究领域:
分析化学
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材料科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）