CURRENT MICROBIOLOGY
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2026
·Vol. 83
·期 4
·207
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IF 2.8
Lu Xie-Feng,Gao Peng,Zhang Shu-Yi,Xu Yuan-Jia,Liu Guo-Hong,Chen Man,Zhou Shun-Gui
Two Gram-stain positive, motile, aerobic, yellowish rod-shaped bacterial strains sgz301303 and sgz301304 T were isolated from the paddy soils. The 16S rRNA gene phylogenetic analysis revealed that both strains belong to the genus Sphingobium , and exhibited the highest sequence similarity (98.3%) with reference strain Sphingobium amiense JCM 11777 T . The draft genome of sgz301304 T has a size of 4.3 Mbp, with DNA G + C content of 64.5%. Whole-genome relatedness analyses showed that the average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain sgz301304 T and S. amiense JCM 11777 T were 82.3% and 25.7%, respectively, both below the accepted species delineation thresholds. The main quinone was Q-10, and the main fatty acids included C 14:0 2-OH (11.3%), and Summed Features 8 (46.6%). Phylogenetic, genomic and phenotypic analyses suggest that strain sgz301304 T belongs to a novel species within the genus of Sphingobium . Therefore, it is recommended to be named as Sphingobium agrisoil sp. nov. The type strain is sgz301304 T (= MCCC 1K09180 T = JCM 37704 T ), respectively.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2012
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IF 2.823
Jian-Dong Cui,Ji Qing Qiu
Curdlan is a microbial polysaccharide composed exclusively of β-(1,3)-linked glucose residues. Until now only bacteria belonging to the Alcaligenes and Agrobacterium species have been reported to produce Curdlan. In this study, a bacterium capable of producing extracellular Curdlan, identified as Pseudomonas sp. on the basis of 16S rDNA gene sequencing, was isolated from soil samples. From the HPLC, permethylation linkage analysis, 13C NMR, and FT-IR analytical data, the polysaccharide consisted exclusively of glucose; the most prominent sugar was 1,3-linked glucose, and most glycosidic bonds joining these sugar residues were of the β-type. This also supported that the exopolysaccharide produced by Pseudomonas sp. was actually Curdlan. In addition, the Pseudomonas sp. was studied for the production of Curdlan by conventional “one-factor-at-a-time technique” and response surface methodology (RSM). It was observed that glucose and yeast extract were the most suitable carbon source and nitrogen source for Curdlan production, respectively. By using RSM, Curdlan production was increased significantly by 188%, from 1.25 to 2.35 g/L, when the strain was cultivated in the optimal condition developed by RSM, and the highest Curdlan production rate of 0.81 g/(L h) was obtained. To the best of the authors' knowledge, this is the first report on Curdlan production by Pseudomonas sp.
研究领域:
微生物学
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生物技术
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化学工程
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JOURNAL OF HAZARDOUS MATERIALS
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2015
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IF 4.529
Shuyan Deng,Yao Chen,Daosheng Wang,Taozhong Shi,Xiangwei Wu,Xin Ma,Xiangqiong Li,Rimao Hua,Xinyun Tang,Qing X. Li
Organophosphorus insecticides have been widely used, which are highly poisonous and cause serious concerns over food safety and environmental pollution. A bacterial strain being capable of degrading O , O -dialkyl phosphorothioate and O , O -dialkyl phosphate insecticides, designated as G1, was isolated from sludge collected at the drain outlet of a chlorpyrifos manufacture plant. Physiological and biochemical characteristics and 16S rDNA gene sequence analysis suggested that strain G1 belongs to the genus Stenotrophomonas . At an initial concentration of 50 mg/L, strain G1 degraded 100% of methyl parathion, methyl paraoxon, diazinon, and phoxim, 95% of parathion, 63% of chlorpyrifos, 38% of profenofos, and 34% of triazophos in 24 h. Orthogonal experiments showed that the optimum conditions were an inoculum volume of 20% (v/v), a substrate concentration of 50 mg/L, and an incubation temperature in 40 °C. p -Nitrophenol was detected as the metabolite of methyl parathion, for which intracellular methyl parathion hydrolase was responsible. Strain G1 can efficiently degrade eight organophosphorus pesticides (OPs) and is a very excellent candidate for applications in OP pollution remediation.
研究领域:
环境微生物学
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污染修复
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农药毒理学
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Acta Pharmaceutica Sinica B
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2022
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IF 14.903
Hangyi Wu,Pei Yang,Aiqin Li,Xin Jin,Zhenhai Zhang,HuiXia Lv
Chronic diabetic wound remains a critical challenge suffering from the complicated negative microenvironments, such as high-glucose, excessive reactive oxygen species (ROS), hypoxia and malnutrition. Unfortunately, few strategies have been developed to ameliorate the multiple microenvironments simultaneously. In this study, Chlorella sp. (Chlorella) hydrogels were prepared against diabetic wounds. In vitro experiments demonstrated that living Chlorella could produce dissolved oxygen by photosynthesis, actively consume glucose and deplete ROS with the inherent antioxidants, during the daytime. At night, Chlorella was inactivated in situ by chlorine dioxide with human-body harmless concentration to utilize its abundant contents. It was verified in vitro that the inactivated-Chlorella could supply nutrition, relieve inflammation and terminate the oxygen-consumption of Chlorella-respiration. The advantages of living Chlorella and its contents were integrated ingeniously. The abovementioned functions were proven to accelerate cell proliferation, migration and angiogenesis in vitro . Then, streptozotocin-induced diabetic mice were employed for further validation. The in vivo outcomes confirmed that Chlorella could ameliorate the undesirable microenvironments, including hypoxia, high-glucose, excessive-ROS and chronic inflammation, thereby synergistically promoting tissue regeneration. Given the results above, Chlorella is considered as a tailor-made therapeutic strategy for diabetic wound healing.
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Chinese Journal of Natural Medicines
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2023
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IF 4.6
Pan TANG,Dan HUANG,Kai-Xuan ZHENG,Dan HU,Ping DAI,Chuan-Hui LI,Sheng-Ying QIN,Guo-Dong CHEN,Xin-Sheng YAO,Hao GAO
From the fungus Trichoderma sp . , we isolated seven novel 18-residue peptaibols, neoatroviridins E-K ( 1−7 ), and six new 14-residue peptaibols, harzianins NPDG J-O ( 8−13 ). Additionally, four previously characterized 18-residue peptaibols neoatroviridins A-D ( 14−17 ) were also identified. The structural configurations of the newly identified peptaibols ( 1−13 ) were determined by comprehensive nuclear magnetic resonance (NMR) and high-resolution electrospray ionization tandem mass spectrometry (HR-ESI-MS/MS) data. Their absolute configurations were further determined using Marfey’s method. Notably, compounds 12 and 13 represent the first 14-residue peptaibols containing an acidic amino acid residue. In antimicrobial assessments, all 18-residue peptaibols ( 1 − 7 , 14 − 17 ) exhibited moderate inhibitory activities against Staphylococcus aureus 209P, with minimum inhibitory concentration (MIC) values ranging from 8−32 μg·mL −1 . Moreover, compound 9 exhibited moderate inhibitory effect on Candida albicans FIM709, with a MIC value of 16 μg·mL −1 .
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Materials Today Communications
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2023
·
IF 3.8
Mihong Cao,Luyi Wang,Qian Zhang,He Zhang,Shengwen Zhong,Jun Chen
In this work, a conductive slurry containing CNTs is used as a modification reagent for the Super-P (SP) conductive agent. After CNTs is recombined with SP, spherical SP particles with a diameter of about 60 nm are evenly dispersed on the surface of CNTs nanotubes with a diameter of 20 nm, which effectively improves the specific surface area of the conductive agent. As a result, the battery using 1.5 % CNTs/SP composite conductive agent shows a smaller EIS impedance value, which makes the comprehensive behaviors of the LiNi 0.5 Co 0.2 Mn 0.3 O 2 battery significantly improved. The CNTs/SP battery shows larger CV curve area and smaller potential difference between oxidation and reduction peaks. In addition, the initial charge and discharge capacity of CNTs/SP battery are 181.7 and 184.7 mAh/g respectively with initial Coulombic efficiency of 98.4%, which are much higher than the pristine SP battery (173.7 and 182.0 mAh/g respectively with initial Coulombic efficiency of 95.4 %). After 70 cycles, the discharge capacity of CNTs/SP battery can still remain at 175.3 mAh/g with capacity retention ratio of 97.2 %, which are also much higher than the pristine SP battery (142.5 mAh/g with capacity retention ratio of 82.8 %). Moreover, compared with the pristine SP batteries, the CNTs/SP batteries show larger discharge capacities at different rates of 0.2 C, 0.5 C, 1.0 C, 2.0 C, 3.0 C and 5.0 C. After combining SP with CNTs nanowires, the discharge capacity, initial coulombic efficiency, cycle stability and rate capability of LiNi 0.5 Co 0.2 Mn 0.3 O 2 electrode are effectively improved, which provides a new idea for the development of novel conductive agent for high-performance cathode materials of lithium ion batteries .
研究领域:
材料科学
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电化学
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能源存储技术
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Catalysts
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2025
·Vol. 15
·期 6
·514
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IF 4
Xinyu Fan,Pengcheng Chen,Dan Wu,Pu Zheng
Phenylalanine butyramide (FBA) is a novel butyric acid derivative with favorable sensory properties, which has broad prospects in medicine and feed processing. However, there is currently limited research on the enzymatic synthesis of FBA. As is well known, lipase plays a crucial role in amide bond synthesis, but it typically requires completely anhydrous conditions. The lipase fromSphingomonassp. HXN-200 (SpL) is the only intracellular lipase identified to date, capable of catalyzing the ammonolysis of esters or acids in an aqueous phase. In this study, we developed a method for the synthesis of FBA catalyzed by SpL in a biphasic reaction system of water and n-hexane. SpL was successfully expressed inE. coliBL21, and the optimal induction conditions were 0.4 mM IPTG and 18 h. It was ascertained that the n-hexane system containing 2% water was conducive to the reaction. Under optimized reaction conditions, 0.89 mg/mL of FBA was obtained within 15 h at 30 °C. Additionally, we found that SpL also has the ability to hydrolyze amides in the reaction of SpL catalyzing the formation of amides, so we further analyzed its catalytic mechanism.
研究领域:
生物化学
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分子生物学
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生物催化
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应用微生物学
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CHEMOSPHERE
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2021
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IF 7.086
Jingwei Wang,Shuang Shan,Qiao Ma,Zhaojing Zhang,Hongsheng Dong,Shuzhen Li,Catherine Sekyerebea Diko,Yuanyuan Qu
Carbon nanotubes (CNTs) have been widely studied because of their potential applications. The increasing applications of CNTs and less known of their environmental fates rise concerns about their safety. In this study, the biotransformation of multi-walled carbon nanotubes (MWCNTs) by Labrys sp. WJW was investigated. Within 16 days, qPCR analysis showed that cell numbers increased 4.92 ± 0.36 folds using 100 mg/L MWCNTs as the sole carbon source. The biotransformation of MWCNTs, which led to morphology and functional group change, was evidenced by transmission electron microscopy and X-ray photoelectron spectroscopy analyses. Raman spectra illustrated that more defects and disordered carbon appeared on MWCNTs during incubation. The underlying biotransformation mechanism of MWCNTs through an extracellular bacterial Fenton-like reaction was demonstrated. In this bacteria-mediated reaction, the OH production was induced by reduction of H 2 O 2 involved a continuous cycle of Fe(II)/Fe(III). Bacterial biotransformation of MWCNTs will provide new insights into the understanding of CNTs bioremediation processes.
研究领域:
环境科学
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纳米技术
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微生物学
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Frontiers in Bioengineering and Biotechnology
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2022
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IF 6.064
Xinming Feng,Yujin Cao,Wei Liu,Mo Xian
Fluoride plays an important role in the fields of materials and medicine. Compared with chemical synthesis, fluorinases are natural catalysts with more application potential, which provide a green and effective way to obtain organofluorine. However, the application of fluorinases is limited by certain factors, such as the limited number of enzymes and their low activity. In this work, two new fluorinases from Amycolatopsis sp. CA-128772 and Methanosaeta sp. PtaU1.Bin055 were identified by gene mining and named Fam and Fme, respectively. The activities of these two enzymes were reported for the first time, and Fme showed good thermal stability, which was different from the reported fluorinases. In addition, the activity toward natural substrate of Fam was improved by site-directed mutagenesis, the catalytic efficiency ( k cat /K m ) of the best mutant containing two amino acid substitutions (T72A and S164G) toward the substrate S-adenosyl-L-methionine was improved by 2.2-fold compared to the wild-type. Structural modeling analysis revealed that the main reason for the increased enzyme activity might be the formation of a new substrate channel. Experimental evidence suggests that the substrate channel may indeed play a key role in regulating the function of the fluorinases.
研究领域:
生物化学
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酶学
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生物催化
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合成生物学
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STEROIDS
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2025
·
IF 2.3
Jiao Yao,Lin-Hong Lu,Sheng-Yun Wei,Ting Wu,Wei Yang,Wei-Hua Wang,Fa-Zhong Yang,Yun-Xian Li,Ping Zhao,Guo-Lei Zhu
Three ergostane-type steroids, including one rare C-29 methylated xylarstane A ( 1 ) and one C22,23-epoxied xylarstane B ( 2 ), along with one reported compound 3 , were isolated from the endophytic fungus Xylaria sp. VDL4 harbored within the medicinal plant Vaccinium dunalianum . The structures of novel compounds were elucidated through comprehensive spectroscopic analysis, DP4 + -validated theoretical 13 C nuclear magnetic resonance (NMR) calculations, and electronic circular dichroism (ECD) calculations. The antifungal activities of the isolates against four phytopathogens were assessed in vitro. Compounds 1 and 3 exerted significant inhibition against Alternaria solani and Botrytis cinerea respectively, both with minimal inhibitory concentration (MIC) of 12.5 μg/mL, comparing with the positive control (Carbendazim and Thiabendazole, MICs = 12.5–25.0 μg/mL)
研究领域:
天然产物化学
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微生物生物技术
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植物病理学
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药用真菌学
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结构鉴定
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BIOCHEMICAL ENGINEERING JOURNAL
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2021
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IF 3.978
Zhikang Deng,Jinyao Zhu,Lie Yang,Zulin Zhang,Bolin Li,Ling Xia,Li Wu
The widespread use of neonicotinoid pesticides has caused environmental threat that has attracted scientific concern. This study developed a microalgae fuel cells (MFCs) with Chlorella sp. as the cathode synergistically enhanced the removal of imidacloprid (IMI). The MFCs were operated at open-circuit and closed-circuit. Under closed-circuit condition (with an external load), 57.20–61.66% of IMI (10–50 mg/L) was removed within 12 days. However, only 39.66–42.66% was removed in the open-circuit (without an external load). Additionally, a voltage of 200 mV was also detected in the closed-circuit MFCs, indicating the green bioenergy was produced during the degradation of IMI. Cyclic voltammetry revealed that the cathodic electroactivity was higher with the addition of IMI. Biomass accumulation (indicated by chlorophyll and dry cell weight) were significantly enhanced by IMI. On the 12th day, there was no difference in MDA content between the different IMI concentration (10–50 mg/L), while a significantly upregulated superoxide dismutase (SOD) of each group was also found at the same time. Six intermediates were detected and five major degradation pathways were proposed. Based on the identified products and microalgae growth, the degradation products of IMI were less toxic /non-toxic than IMI. Considering the efficient removal of IMI and energy production, the MFCs could be a promising technique for controlling IMI contamination by effective detoxification.
研究领域:
环境科学
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生物技术
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电化学
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毒理学
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Molecular Catalysis
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2026
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IF 4.3
Kang An,Guoguo Wang,Lulu Zhang,Xuefeng Zhang,Jinchu Yang,Yongming Xu,Zhenzhen Huang,Jun Xi,Jun Meng,Hua Li,Beibei Zhao,Nannan Wang
Natural carvone is widely utilized in the food industry, cosmetics, agrochemicals, and pharmaceuticals due to its unique sensory characteristics and numerous biological effects. The enzyme carveol dehydrogenase catalyzes the oxidation reaction transforming carveol into carvone. In the present study, genome-based analysis of Klebsiella sp. O852 enabled the identification of four potential alcohol dehydrogenase (ADH) genes involved in the bioconversion process from carveol to carvone. These candidate genes were then heterologously expressed in Escherichia coli BL21(DE3), followed by purification and biochemical characterization of the recombinant proteins. All KlebADHs (ADHs from Klebsiella sp. O852) except KlebADH4 demonstrated carveol dehydrogenase activity and effectively catalyzed the conversion of carveol into carvone. Enzymatic experiments revealed optimal temperatures of 30–40 °C and an optimal pH of 9.0 for KlebADH3, KlebADH5, and KlebADH6. These enzymes were active with both NADP + and NAD + . Some organic solvents and metal ions, particularly Cu 2+ and Mn 2+ , significantly inhibited enzyme activity. Additionally, kinetic experiments indicated that KlebADH3 possessed the highest catalytic efficiency. This study expands the enzyme resources available for synthesizing carvone-derived compounds and provides a theoretical foundation for regulating enzymatic catalytic properties and improving carvone production.
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