BIOORGANIC CHEMISTRY
·
2025
·
IF 4.7
Hanbin Deng,Shanyu Yang,Huwen Wu,Han Zhou,Peishen Zhang,Shaowen Cheng,Fabiao Yu,Rui Wang
In the complex process of wound healing, real-time and dynamic monitoring of key indicators such as pH and reactive oxygen species (ROS) in the microenvironment can effectively evaluate the inflammatory status and therapeutic effect of the wound. In this study, a dual-responsive surface-enhanced Raman scattering (SERS) probe was developed, which could simultaneously detect the changes of pH and ROS levels during the wound healing process. The gold–silver core–shell nanoparticles (Au@Ag NPs) were utilized as the active substrate, and 4-mercaptobenzoic acid (4-MBA) and 2,5-dihydroxythiophenol (DHBT) were modified as Raman reporter molecules, which were further coated with polyethylene glycol and bovine serum albumin to improve the biocompatibility and stability. In the Raman spectra, the peak of DHBT at 876 cm −1 was used to indicate the ROS level, while the intensity ratio at 1360 and 1075 cm −1 (I 1360 /I 1075 ) of 4-MBA reflected the pH change. The SERS probe exhibited good ratiometric response characteristics, high selectivity, and low cytotoxicity. In vitro SERS imaging experiments demonstrated that the probe could dynamically monitor ROS generation and pH changes, while SERS imaging in the mouse wound model further confirmed its ability to monitor pH and ROS in the wound microenvironment in vivo. Histological analysis also verified its biosafety. In summary, the dual-responsive SERS probe designed in this study has potential application value in evaluating wound healing effects and provides a new approach for monitoring the wound microenvironment.
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EUROPEAN POLYMER JOURNAL
·
2025
·
IF 6.3
Ke Wang,Xiaofang Liu,Ziyan Li,Meng Zhao,Xuan Pu,Qingpei Guan,Yongfei Zeng
Here, we prepared three types of temperature/pH/redox-responsive diblock copolymers with similar molecular weights, using poly(2-((acrylamidomethyl)(methyl)amino)ethyl ferrocene formate) (P(Fc-N-AM)) as the hydrophobic block and poly( N -isopropylacrylamide) (PNIPAM), poly(ethylene glycol) (PEG), poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) as the hydrophilic block, respectively. Subsequently, their triple-responsive properties were characterized. For temperature-response, they exhibited a lower critical solution temperature (LCST)-type cloud point (CP) in water, and an upper critical solution temperature (UCST)-type CP in ethanol and propanol, and the CP temperature was influenced by the molecular weight and type of the hydrophilic block. For pH-response, all copolymers showed a critical pH in water, and when the molecular weights were similar, their critical pH was also close. The redox-response was confirmed by the reversible change of the characteristic peak of ferrocene (Fc) upon the addition of redox reagents to the solution. Subsequently, these copolymers self-assembled in water to form spherical micelles with different sizes, which also possessed triple stimuli-responsive properties. Finally, by encapsulating Nile Red (NR), the release behavior of NR-loaded micelles under heating, the addition of acid and oxidant were tested. The above studies have enriched the types of temperature/pH/redox triple-responsive polymers, revealed the influence of hydrophilic blocks on the responsive properties and self-assembly behavior of copolymers, and indicated that these copolymers have promising applications in the fields of self-assembly and controlled release.
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CHEMICAL ENGINEERING SCIENCE
·
2025
·
IF 4.3
Wei Hu,Jun Zhang,Changdong Sheng
Industrial wastewater containing hexavalent chromium (Cr(VI)) and methyl orange (MO) poses severe threats to aquatic ecosystems due to their high toxicity and difficulty in degradation. In this study, a microfibrillated cellulose (MFC)-reinforced polyethyleneimine (PEI) interpenetrating network cryogel was fabricated and further modified through a two-step synergistic quaternization process. The mechanical properties of the modified cryogel were validated using a universal testing machine, which showed a five-fold enhancement compared to its non-reinforced counterparts. The dynamic column adsorption behavior of the quaternized PEI/MFC cryogel at neutral pH was systematically investigated. For individual contaminant systems, the operational parameters were investigated, including flow rate, initial concentration, and bed height. In the mixed solution, a competitive adsorption relationship was observed between Cr(VI) and MO, wherein Cr(VI) preferentially occupied binding sites owing to the strong affinity of quaternary ammonium groups, resulting in a distinctive “peak–plateau” phenomenon in the breakthrough curves for MO. Breakthrough curves were analyzed using Thomas, bed depth service time (BDST), and Yoon–Nelson kinetic models, all of which exhibited strong agreement with the experimental results ( R 2 > 0.96), confirming that these models were well-suited for the design of this column. The regeneration performance of the adsorbent was evaluated using chemical regeneration methods. Based on FTIR and XPS analyses, the adsorption mechanisms can be summarized as follows: MO is captured by the cryogel mainly via ion exchange and hydrogen bonding, whereas Cr(VI) undergoes synergistic ion exchange and redox reactions. The study confirms the applicability of the synthesized cryogel as an effective adsorbent in mitigating pollutants.
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PHYTOMEDICINE
·
2025
·
IF 8.3
Jinjin Yu,Xinya Zhang,Xinyao Liu,Lingli Li,Huixin Song,Yajing Ma,Lingyi Liu,Yuzhi Luo,Sha Wen,Songyuan Xia,Ruisi Zhu,Jingyu Wang,Dezhu Zhang,Jianguo Meng,Weifeng Li,Xiaofeng Niu
Background Atherosclerosis (As) is a chronic vascular inflammatory disease, which has become a major global public health challenge. Therefore, it is urgent to find natural anti-As active ingredients with high efficacy and low toxicity and develop a suitable delivery system to prevent and treat AS. Schisandra chinensis (Sch) has attracted much attention because of its rich anti-inflammatory active compounds, but its targeted delivery and bioavailability in vivo are still problems to be solved urgently. Objective The purpose of this study is to screen the effective components of Sch with significant therapeutic effect on AS, and to construct a dual-response nano-carrier of pH‑ROS to realize accurate drug release and targeted therapy. Methods The experimental research design was employed by integrating network pharmacological prediction, molecular docking verification, in vitro cell model and in vivo animal model. Results Through network pharmacology and molecular docking technology, we first identified schizandrol A (Sch A) as the main active ingredient against As. Under the condition of a feed ratio of 6:4, the double-response nanoparticles showed excellent physical and chemical properties: the average particle size was 234.9 nm, the drug loading was 19.88%, and the encapsulation efficiency was 62.06%. In vitro biological evaluation demonstrated that the nanoparticles loaded with Sch A exerted a strong anti‑steatosis effect by inhibiting the PI3K/AKT/SREBP-1 signaling pathway, thus reducing intracellular lipid synthesis and inflammatory responses. In vivo experiments revealed that the nanoparticles displayed good biocompatibility and targeting ability, effectively reducing plaque area in the aorta, enhancing plaque stability, and significantly lowering blood lipid levels in mice. Conclusions In summary, Sch A is an effective component for anti-As, and the constructed pH-ROS dual-responsive nanoparticles demonstrate good biological safety and targeting both in vitro and in vivo , indicating their potential application in the treatment of As.
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LANGMUIR
·
2025
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IF 3.9
Haiqian Zhao,Fang Chen,Minghong Feng,Wenjing Lin,Hangbo Yue,Jianwei Guo,Chufen Yang
To improve the stability and drug-controlled release capability of folic acid-modified pH-responsive polymeric mixed micelles FA-PEG-PHEMA-PDEAEMA (FA-P1)/PCL-PHEMA-PPEGMA (P2), the hydroxyl termini of the PHEMA blocks in polymers FA-P1 and P2 were esterified with selenooctanoic acid (SA) to produce FA-P1-SA and P2-SA, which were capable of self-cross-linking and exhibited reduction responsiveness. Thus, folic acid-modified dual-responsive diselenide-containing mixed cross-linked micelles composed of FA-P1-SA and P2-SA were constructed and employed for the delivery of doxorubicin (DOX). Structural characterization by 1H NMR, FT-IR, Raman spectroscopy, and GPC confirmed successful SA conjugation at an esterification degree of approximately 70%. Experimental and dissipative particle dynamics (DPD) analyses showed that the DOX loading content and encapsulation efficiency of the FA-containing mixed cross-linked micelles (MCLM@DOX) (1:1 mass ratio of FA-P1-SA/P2-SA) were slightly lower than those of non-cross-linked micelles (MMSA@DOX), yet afforded superior retention under physiological conditions (16.11% vs 22.49% release at 120 h) and rapid, stimuli-triggered release (75.20% vs 83.32%) in reductive, acidic conditions. Cytotoxicity assays indicated minimal polymer toxicity after SA modification. Furthermore, FA-containing MCLM@DOX exhibited relatively good tumor-targeting and anticancer efficacy compared with FA-free counterparts (MCLM1@DOX).
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Jiawei Zhang,Kaili Zhang,Chen Tian,Gaoyue Zhang,Xiao Jiang,Kui Wang,Kang Sun,Jianchun Jiang
Kraft lignin (KL), a major by-product of the pulp and paper industry, has garnered significant research attention due to its abundant availability and distinctive chemical structure, making it a promising candidate for applications such as biofuels, carbon materials, and polymer composites. Despite these advantages, its utilization in fine chemical industries, including nanomaterials, is constrained by its inherent dark color and broad molecular weight distribution. Addressing these limitations has become a central focus in ongoing research efforts aimed at enhancing the functionality and commercial viability of KL. In this study, we fractionated KL from a paper-making enterprise using pH fractionation, and obtained four kinds of lignin with different molecular weights. Following that, lignin microspheres (LMPs) were formed by self-assembly from four sizes of KL. Analyzing the KL and LMP properties, the low molecular weight lignin, such as KL-2, exhibited good antioxidant properties (84.27 %, 5 mg/mL), high brightness (ISO% = 7.6), high L ⁎ value (L ⁎ = 86.2) and low polydispersity index (PDI = 1.19). The LMP showed a narrow size distribution (1.0–1.4 μm). When the LMP content is 5 %, the 5 % LMP/PVA film maintains high transparency and exhibits excellent UV protection, achieving a UV shielding rate of up to 94.6 %. Therefore, this study offered an effective way for the comprehensive utilization of pulping waste KL.
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ACS Sustainable Chemistry & Engineering
·
2025
·
IF 7.3
Huaying Zhong,Run Liu,Huihui Liu,Yi Zhang,Yihan Liu,Yufei Zhang,Mingming Zheng
A novel pH-responsive Pickering interfacial biocatalysis (PIB) system has been constructed. The oil-in-water emulsion was stabilized by immobilized enzyme CL@HMSS-DMAEMA, which was obtained through physical adsorption of lipase CL onto hollow mesoporous silicon spheres modified with 2-[dimethylamino]ethyl methacrylate (HMSS-DMAEMA). Within the pH response range of 3.0–11.0, the zeta potentials of the carrier and lipase exhibited opposite charges and almost mirror-symmetrical configuration, thereby ensuring electrostatic attraction throughout the demulsification/emulsification process. This strategy significantly enhanced enzyme loading, thermal stability, enzyme activity, and pH-responsive resilience. The pH-responsive PIB system achieved a 95.0% conversion rate for retinol fatty acid esters, with a catalytic efficiency (CE) 14.8 times higher than that of the PE system (CL@HMSS) and 25.2 times higher than that of free enzymes, while maintaining over an 80.0% conversion rate after 10 cycles. These findings underscore the importance of electrostatic interactions in enzyme immobilization, thereby improving the reliability of pH-responsive PIB platforms. The present study also proposes a novel design concept for the stimulus-response PIB system.
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WATER RESEARCH
·
2020
·
IF 9.13
Hankun Yang,Wu Xue,Mengjie Liu,Kai Yu,Wenzheng Yu
Oxidation is a widely used method in drinking water treatment to mitigate the membrane fouling caused by the natural organic matters (NOM) from the surface water during ultra-filtration (UF) and nano-filtration (NF) processes, and H 2 O 2 is one of the common oxidants for it. However, the oxidation capability of H 2 O 2 at neutral pH is lower, compared to the acidic and alkaline conditions. In order to improve the efficiency of NOM oxidation at neutral pH, a carbon-doped Fe 3 O 4 peroxidase-like nanozyme (CFPN) was synthesized in this study and used as a high-performance catalyst for H 2 O 2 to generate hydroxyl radical . The oxygen-containing groups on the carbon structure of CFPN can form an acidic microenvironment, allowing H 2 O 2 to produce hydroxyl radical by catalysis in neutral conditions. The results of hydrophilicity analysis, zeta potential , high-performance liquid size exclusion chromatography (HPSEC), Fourier transform infrared spectrum (FTIR) and flux indicated that the hydroxyl radical can oxidize the hydrophobic matters of humic acid (HA) into hydrophilic matters by Fenton reaction or electrophilic addition reaction, which can mitigate the fouling of NF membranes. The results of the same test for the bovine serum albumin (BSA) indicated that the hydroxyl radical can mitigate the fouling of UF membranes by degrading the tertiary and secondary structures of BSA and partly oxidizing the side chain groups. In addition, two types of surface water samples were used to verify the above mechanism, and the results indicated that the hydroxyl radical treatment at neutral pH is a new viable and effective strategy to significantly mitigate the NOM fouling of UF and NF membranes.
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ACS Applied Materials & Interfaces
·
2016
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IF 7.145
Zhao Dang,Libin Liu,Yan Li,Yu Xiang,Gailan Guo
The development of stimuli-responsive materials with the ability of controllable oil/water separation is crucial for practical applications. Here, a novel pH responsive nonfluorine-containing copolymer was designed. The copolymer together with silica can be dip-coated on different materials including cotton fabric, filter paper, and polyurethane foam. The coated materials exhibit switchable superhydrophilicity and superhydrophobicity and can be applied in continuous separation of oil/water/oil three phase mixtures, different surfactant stabilized emulsion (oil-in-water, water-in-oil, and oil-in-acidic water) as well as oil uptake and release via in situ and ex situ pH change. We expect that the coatings highlight the practical applications because of the cost-effective preparation process and fluorine-free strategy.
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FOOD HYDROCOLLOIDS
·
2024
·
IF 11
Mingxia Wang,Yaqian Li,Yuxuan Zhao,Huiling Gao,Zhaochu Xu,Liang Chen,Jianzhang Liu,Hao Liang
As a typical biofilm disease, dental caries is a public healthcare problem and high sugar diets increase the risk of dental caries. Cationic nanomaterials play an important role in targeting and removing of biofilms, but their negative effects on normal cells have always caused widespread concern. In this work, pH-triggered charge-switching nanoparticles (SC&CS NPs) with selectable targeting were skillfully constructed by electrostatic adsorption and chemical cross-linking of sodium caseinate (SC) and chitosan (CS) to develop functional foods. SC&CS NPs were negatively charged and stable at pH 7.4. Once encountering in acidic microenvironment below pH 5.7, the potential of SC&CS NPs converted to positive. Based on this property, SC&CS NPs could identify the infected sites of biofilms to selectively target bacteria, while not interacting with cells in the normal physiological environment, thereby avoiding damage to normal cells. Besides, SC&CS NPs were excellent delivery systems that could effectively encapsulate bioactive ingredients with high loading efficiency (12.16 ± 0.04%) and release bioactive ingredients on demand. Moreover, after being encapsulated by SC&CS NPs, the antibacterial and anti-biofilm activities of bioactive ingredients were significantly improved. The charge-switching nanomaterials are expected to provide a safer, more effective, and smarter strategy for functional foods.
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RSC Advances
·
2020
·Vol. 10
·期 61
·36971-36979
·
IF 3.119
Hua Tian,Yongcheng Dai,Wenzhe Fu,Haifang Liu,Mengting Li,Meiyuan Lv,Xueqiong Yin
In this work, a multifunctional ratiometric fluorescence (FL) nanohybrid (CSCDs@DC) was synthesized from chitosan based carbon dots (CSCDs) and dansyl chloride (DC) at room temperature. The CSCDs@DC revealed strong FL intensity, great stability and excellent anti-photobleaching properties. Herein, CSCDs@DC was responsive to pH value in the range of 1.5–4.0 and exhibited color-switchable FL properties between acidic and alkaline environments. In addition, CSCDs@DC showed good selectivity and sensitivity towards Fe3+ ions. A good linear relationship for the Fe3+ ion detection was obtained in the range from 0 μM to 100 μM, with a detection limit of 1.23 μM. What's more, CSCDs@DC can be used as a fluorescent ink. It expressed superior optical properties after 3 months of storage or continuous exposure to UV light for 24 h. This study suggested that CSCDs@DC had potential in the detection of pH and metal ions, as well as showing promising application in the anti-counterfeiting field.
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JOURNAL OF FLUORESCENCE
·
2024
·
IF 2.6
Guo Shanshan,Li Hua
Diabetes, particularly Type 2 Diabetes Mellitus (T 2 DM), is a chronic metabolic disorder with high and increasing global prevalence, characterized by insulin resistance and inadequate insulin secretion. Despite advancements in novel drug delivery systems, widespread and systematic treatment of advanced glycation end products (AGEs) remains challenging due to issues like drug toxicity, low water solubility, and uncontrolled release. Thus, developing nanoplatforms with controlled release capabilities has become a major research focus. Due to its excellent biocompatibility and drug delivery properties, chitosan has attracted considerable attention as a typical biopolymer. In this study, we designed and synthesized an intelligent fluorescence-pH sensitive nanopolymer material using chitosan. We loaded drug 1 and chromium phthalocyanine (CrPc) into folic acid-conjugated carboxymethyl chitosan (FA-CMCS) nanocarriers, forming FA-CMCS@1-CrPc. Comprehensive characterization of FA-CMCS@1-CrPc was conducted using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), and gas adsorption analysis (BET). The results indicate that the nanomaterial was successfully synthesized and exhibits excellent specific surface area, biocompatibility, and fluorescence response. Further research revealed that FA-CMCS@1-CrPc not only achieved controlled drug release but also could regulate drug release by adjusting pH. Additionally, due to its strong fluorescence performance, the nanomaterial demonstrated higher detection sensitivity, especially for monitoring the release of 5% trace drugs. An in vitro model of insulin-resistant cells was established to evaluate the effects of the drug delivery system on glucose degradation and AGE-RAGE regulation, providing a foundation for the development of new T 2 DM drugs. Graphical Abstract
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