PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION
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2015
·Vol. 33
·期 1
·53-62
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IF 3.081
Zhenglin Li,Ying Hu,Tingting Jiang,Kenneth A. Howard,Yonggang Li,Xuelei Fan,Ye Sun,Flemming Besenbacher,Miao Yu
Constructing novel multimodal antitumor therapeutic nanoagents has attracted tremendous recent attention. In this work, a new drug-delivery vehicle based on human-serum-albumin (HSA)-coated Prussian blue nanoparticles (PB NPs) is synthesized. It is demonstrated that doxorubicin (DOX)/HSA is successfully loaded after in situ polymerization of dopamine onto PB NPs, and the PB@PDA/DOX/HSA NPs are highly compatible and stable in various physiological solutions. The NPs possess strong near-infrared (NIR) absorbance, and excellent capability and stability of photothermal conversion for highly efficient photothermal therapy applications. Furthermore, a bimodal on-demand drug release sensitively triggered by pH or NIR irradiation has been realized, resulting in a significant chemotherapeutic effect due to the preferential uptake and internalization of the NPs by cancer cells. Importantly, the thermochemotherapy efficacy of the NPs has been examined by a cell viability assay, revealing a remarkably superior synergistic anticancer effect over either monotherapy. Such multifunctional drug-delivery systems composed of approved materials may have promising biomedical applications for antitumor therapy.
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JOURNAL OF CONTROLLED RELEASE
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2025
·
IF 11.5
Xiang-Yun Du,Yin-Jian Song,Chen Du,Ji-Dong Liu
Delayed skin wound healing caused by bacterial infection and persistent oxidative stress has been considered as a frequently encountered clinical problem that significantly affects patients' health. However, the real-time monitoring and on-demand treatment of non-healing wounds remain a great challenge. Herein, a feasible strategy is developed to core-shell polymer/hydrogel microfibers by integrating microfluidic spinning with shear-flow-induced coating technology. The fabricated self-healing microfibers are facilely assembled into nonwoven fabrics through reversible physical interactions. The core polycaprolactone (PCL) acting as a skeleton endows fabrics with structural and mechanical robustness (4.67 MPa), while the hydrogel shell brings functional diversity including great flexibility, self-healing, self-adhesion and pH-responsive swelling capability. By introducing epigallocatechin gallate (EGCG) in core PCL phase, the remarkable reactive oxygen species (ROS) cleaning capacity (93.8 % for 0.5 h) and antibacterial activity against E. coli (98.7 %) and S. aureus (99.6 %) are achieved. Particularly, the pH-regulated drug release of fabric in a prolonged form is achieved owing to the reversibly swelling/shrinking behavior of hydrogel shell with the changed environmental pH, which is expected to realize on-demand drug delivery and treatment of wound. Additionally, the pH-responsive curcumin-loaded mesoporous microparticles are incorporated into hydrogel shell, aiming to visually real-time detect pH level in a high-efficiency and reversible manner. Moreover, the fabric can serve as a flexible wearable sensor for precisely monitoring motions of patients. This strategy explores a feasible solution to diligently tracking wound pH and movements, along with inhibiting wound infection, substantiating a great foundation for application in personalized, intelligent and precise theranostic wound dressings.
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BIOSENSORS & BIOELECTRONICS
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2025
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IF 10.5
Shixin Cai,Rongfeng Cai,Sanxia Wang,Xin Chen,Lele Zhang,Yunpeng Zhao,Xiaoli Wang,Yuting Zhang,Nandi Zhou
Circulating tumor cells (CTCs) are closely associated with cancer progression and metastasis, making the development of precise CTCs enumeration technology crucial for real-time cancer monitoring and post-operative treatment. Researches indicated that miRNA-21 exhibits abnormally high expression in CTCs and can thus serve as a biomarker for CTCs profiling. Herein, an innovative dual-responsive detection platform was developed by integrating miRNA-21/pH-activated ZnO@Au NPs-hairpin DNA (HP) nanoprobes with microfluidic single-cell droplet technology for CTCs identification. In this platform, ZnO@Au NPs-HP nanoprobes and CTCs are co-encapsulated into single-cell droplets, after which the nanoprobes enter CTCs. Leveraging the characteristic intracellular high miRNA-21 level and acidic microenvironment of CTCs, Zn-DNAzyme contained in HP can be activated to cleave its substrate chain and the fluorescence is thus restored. This strategy effectively avoids the interference from free miRNA-21 in blood and enhances detection specificity. Subsequent droplet fluorescence detection enables the accurate CTCs quantification. The platform enables robust detection and precise isolation of CTCs in blood samples from three distinct cancer cell types HCT116, HepG2 and HeLa, and achieves remarkably high sensitivity with successful recovery of 5 CTCs/mL in spiked human blood samples. Furthermore, the platform has been successfully applied for the accurate counting of CTCs in whole blood samples from patients with three types of cancers, i.e. colorectal cancer, hepatocellular carcinoma, and cervical cancer. Therefore, the established system exhibits superior detection performance and significant clinical potential, providing a robust tool for liquid biopsy and personalized cancer management.
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LANGMUIR
·
2025
·
IF 3.9
Haiqian Zhao,Fang Chen,Minghong Feng,Wenjing Lin,Hangbo Yue,Jianwei Guo,Chufen Yang
To improve the stability and drug-controlled release capability of folic acid-modified pH-responsive polymeric mixed micelles FA-PEG-PHEMA-PDEAEMA (FA-P1)/PCL-PHEMA-PPEGMA (P2), the hydroxyl termini of the PHEMA blocks in polymers FA-P1 and P2 were esterified with selenooctanoic acid (SA) to produce FA-P1-SA and P2-SA, which were capable of self-cross-linking and exhibited reduction responsiveness. Thus, folic acid-modified dual-responsive diselenide-containing mixed cross-linked micelles composed of FA-P1-SA and P2-SA were constructed and employed for the delivery of doxorubicin (DOX). Structural characterization by 1H NMR, FT-IR, Raman spectroscopy, and GPC confirmed successful SA conjugation at an esterification degree of approximately 70%. Experimental and dissipative particle dynamics (DPD) analyses showed that the DOX loading content and encapsulation efficiency of the FA-containing mixed cross-linked micelles (MCLM@DOX) (1:1 mass ratio of FA-P1-SA/P2-SA) were slightly lower than those of non-cross-linked micelles (MMSA@DOX), yet afforded superior retention under physiological conditions (16.11% vs 22.49% release at 120 h) and rapid, stimuli-triggered release (75.20% vs 83.32%) in reductive, acidic conditions. Cytotoxicity assays indicated minimal polymer toxicity after SA modification. Furthermore, FA-containing MCLM@DOX exhibited relatively good tumor-targeting and anticancer efficacy compared with FA-free counterparts (MCLM1@DOX).
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BIOORGANIC CHEMISTRY
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2025
·
IF 4.7
Hanbin Deng,Shanyu Yang,Huwen Wu,Han Zhou,Peishen Zhang,Shaowen Cheng,Fabiao Yu,Rui Wang
In the complex process of wound healing, real-time and dynamic monitoring of key indicators such as pH and reactive oxygen species (ROS) in the microenvironment can effectively evaluate the inflammatory status and therapeutic effect of the wound. In this study, a dual-responsive surface-enhanced Raman scattering (SERS) probe was developed, which could simultaneously detect the changes of pH and ROS levels during the wound healing process. The gold–silver core–shell nanoparticles (Au@Ag NPs) were utilized as the active substrate, and 4-mercaptobenzoic acid (4-MBA) and 2,5-dihydroxythiophenol (DHBT) were modified as Raman reporter molecules, which were further coated with polyethylene glycol and bovine serum albumin to improve the biocompatibility and stability. In the Raman spectra, the peak of DHBT at 876 cm −1 was used to indicate the ROS level, while the intensity ratio at 1360 and 1075 cm −1 (I 1360 /I 1075 ) of 4-MBA reflected the pH change. The SERS probe exhibited good ratiometric response characteristics, high selectivity, and low cytotoxicity. In vitro SERS imaging experiments demonstrated that the probe could dynamically monitor ROS generation and pH changes, while SERS imaging in the mouse wound model further confirmed its ability to monitor pH and ROS in the wound microenvironment in vivo. Histological analysis also verified its biosafety. In summary, the dual-responsive SERS probe designed in this study has potential application value in evaluating wound healing effects and provides a new approach for monitoring the wound microenvironment.
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DYES AND PIGMENTS
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2025
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IF 4.2
Xin Hu,Shaorong Huang,Tongsheng Huang,Shiyu Huang,Lihua Chen,Weifeng Zhu,Xiancai Li,Meiying Liu,Xiaoyong Zhang,Yen Wei
Developing efficient drug delivery systems (DDS) is crucial for cancer therapy. Herein, we report the construction of a pH-responsive DDS using an aggregation-induced emission (AIE)-active carrier (termed as TPA-CHTAD-PEG-DA), which is engineered by integrating dopamine (DA) on the side chains of AIE-active TPA-CHTAD-PEG copolymer. TPA-CHTAD-PEG-DA pendant with DA can form dynamic catechol-boronate ester with anti-cancer drug bortezomib (BTZ) to form the aimed DDS (TCP-DA-BTZ). We demonstrated that TCP-DA-BTZ can self-assemble into nanoparticles with high water dispersibility, intense fluorescence and high drug-loading capacity (15.5 %). The DDS showed a significant pH-responsive drug release profile, with minimal leakage under physiological pH (26.3 % at pH 7.4) but rapid drug release under acidic conditions (50 % at pH 5.0). In vitro experiments suggested TPA-CHTAD-PEG could be efficiently internalized by cancer cells and exhibited neglected cytotoxicity, while TCP-DA-BTZ exerted considerable or even stronger cell killing ability towards MDA-MB-231 cells compared with free BTZ. Considering the above aspects, such as high drug loading capacity, pH-responsive drug release, and intrinsic imaging capabilities, the AIE-active pH-responsive DDS developed in this work is expected to overcome limitations (e.g., off-target toxicity and inadequate spatiotemporal control) of conventional chemotherapy, holding great potential for fluorescence-guided cancer theranostics.
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SENSORS AND ACTUATORS B-CHEMICAL
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2024
·
IF 8
Xiaoyan Huang,Wanlu Sun,Yanyan Zhu,Chao Zhao,Baojin Tan,Hai-Yan Wang,Yi Liu
Photodynamic therapy (PDT) could induce apoptosis by releasing reactive oxygen species (ROS) to treat tumor. Application of pH/light dual-activated nanoparticles could generate ROS in the acidic tumor microenvironment under 808 nm laser irradiation for precise treatment. L-arginine (L-Arg) triggered by ROS is an efficient nitric oxide (NO) donor for gas therapy. In addition, it is reported that peroxynitrite (ONOO - ) produced by rapid reaction of ROS and NO could sensitize PDT and induce apoptosis for synergistic treatment. Herein, we developed the L-Arg loaded pH/light dual-activated PEGylated photosensitizer for self-assembly into nanoparticles named Rho-Arg. As measured by fluorescence spectroscopy, the pKa of Rho-Arg was 5.5, which is suitable for applying in the tumor microenvironment. The nanoplatform was firstly activated under the acidic condition of tumor, and then illuminated by 808 nm laser to controllably release ROS, and sequentially produced NO and ONOO - . In conclusion, the Rho-Arg nanoplatform may provide a promising synergistic therapy tool for tumor treatment.
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TALANTA
·
2016
·
IF 4.035
Mingtai Sun,Libo Du,Huan Yu,Kui Zhang,Yang Liu,Suhua Wang
It is crucial to monitor intracellular pH values and their fluctuation since the organelles of cells have different pH distribution. Herein we construct a new small molecule fluorescent probe HBT-O for monitoring the subtle pH values within the scope of neutral to acid in living cells. The probe exhibited good water solubility, a marked turquoise to olivine emission color change in response to pH, and tremendous fluorescence hypochromatic shift of ∼50 nm (1718 cm −1 ) as well as the increased fluorescence intensity when the pH value changed from neutral to acid. Thus, the probe HBT-O can distinguish the subtle changes in the range of normal pH values from neutral to acid with significant fluorescence changes. These properties can be attributed to the intramolecular charge transfer (ICT) process of the probe upon protonation in buffer solutions at varied pH values. Moreover, the probe was reversible and nearly non-toxic for living cells. Then the probe was successfully used to detect pH fluctuation in living cells by exhibiting different fluorescence colors and intensity. These findings demonstrate that the probe will find useful applications in biology and biomedical research.
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Biotechnology for Biofuels and Bioproducts
·
2022
·Vol. 15
·期 1
·1-10
·
IF 0
Hua Xia,Zhang ChenHui,Han Jian,Xu Yong
Background Hydroxyl acid is an important platform chemical that covers many industrial applications due to its dual functional modules. At present, the traditional technology for hydroxyl acid production mainly adopts the petroleum route with benzene, cyclohexane, butadiene and other non-renewable resources as raw materials which violates the development law of green chemistry. Conversely, it is well-known that biotechnology and bioengineering techniques possess several advantages over chemical methods, such as moderate reaction conditions, high chemical selectivity, and environmental-friendly. However, compared with chemical engineering, there are still some major obstacles in the industrial application of biotechnology. The critical issue of the competitiveness between bioengineering and chemical engineering is products titer and volume productivity. Therefore, based on the importance of hydroxyl acids in many fields, exploring a clean, practical and environmental-friendly preparation process of the hydroxyl acids is the core purpose of this study. Results To obtain high-purity hydroxyl acid, a microbiological regulation for its bioproduction by Gluconobacter oxydans was constructed. In the study, we found a critical point of chain length determine the end-products. Gluconobacter oxydans catalyzed diols with chain length ≤ 4, forming hydroxyl acids, and converting 1,5-pentylene glycol and 1,6-hexylene glycol to diacids. Based on this principle, we successfully synthesized 75.3 g/L glycolic acid, 83.2 g/L 3-hydroxypropionic acid, and 94.3 g/L 4-hydroxybutyric acid within 48 h. Furthermore, we directionally controlled the products of C5/C6 diols by adjusting pH, resulting in 102.3 g/L 5‑hydroxyvaleric acid and 48.8 g/L 6-hydroxycaproic acid instead of diacids. Combining pH regulation and cell-recycling technology in sealed-oxygen supply bioreactor, we prepared 271.4 g 5‑hydroxyvaleric acid and 129.4 g 6-hydroxycaproic acid in 6 rounds. Conclusions In this study, a green scheme of employing G. oxydans as biocatalyst for superior-quality hydroxyl acids (C2–C6) production is raised up. The proposed strategy commendably demonstrated a novel technology with simple pH regulation for high-value production of hydroxyl acids via green bioprocess developments.
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FIBERS AND POLYMERS
·
2016
·Vol. 17
·期 10
·1531-1539
·
IF 1.022
Mao Yanli,Cui Jiuyun,Zhao Juan,Wu Yilin,Wang Chen,Lu Jian,Lin Xinyu,Yan Yongsheng
Via Pickering emulsion polymerization, pH-sensitive magnetic molecularly imprinted polymers (HM-MIPs) were synthesized using bifenthrin (BF) as the template molecule, and pH-sensitive monomer methacrylic acid as the functional monomer. Characterizations of HM-MIPs were achieved by FTIR, TGA, TEM, SEM, and VSM, and the results indicated that HM-MIPs exhibited magnetic property (Ms=1.06 emu/g). HM-MIPs were acted as the adsorbent for a series of adsorption performance testing of BF. Equilibrium data were described by the Langmuir and Freundlich isotherm models. Kinetic properties were successfully investigated by pseudo-first-order model, pseudo-second-order model. The selective recognition experiments exhibited outstanding selectively adsorption effect of the HM-MIPs for target BF over diethyl phthalate and fenvalerate. In water, the HM-MIPs could achieve the adsorption and release of BF by controlling the pH value of the aqueous solution. The results indicated that the molecularly imprinted polymers exhibited good adsorption, selectivity and pH-sensitive performance for BF.
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POLYMER
·
2016
·
IF 3.586
Gang Li,Guoping Zhang,Rong Sun,Ching-Ping Wong
Via the radical polymerization of 2-(dimthylamino) ethyl methacrylate in the polyacrylic acid solution, we obtain the dually pH-reponsive polyelectrolytes complex hydrogel, which could be triggered to be swollen either in acid and basic environments. The results show that the responsive behavior of these PEC hydrogels is insensitive to monomer concentration in the prepolymerization, but can be tuned by changing the charge ratio or introducing chemical crosslinker. Moreover, other amazing propertise of the prepared PEC hydrogel are presented, which are including the ionic strength-responsiveness, the modest mechanical property (a tensile stress of 0.2 MPa and a tensile strain of 1500%) and the self-healing ability (a self-healing efficiency of ca. 70%). In brief, we report a multi-functional hydrogel which might serve as a new platform for responsive materials.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Anqi Yang,Yan Huang,Songbao Fu,Haodong Zhang,Sailing He
A high-precision pH monitoring system over a wide pH range is introduced. The system comprises a cavity-enhancement module constructed by two high-reflectivity mirrors, a microfluidic pH sensing chip based on a binary-indicator membrane of Congo red and m-cresol purple, and a hyperspectral transmission module. This structure extends the effective absorption optical path of the sensing chip, significantly amplifying the spectral differences at various pH values. The spectrum of the transmitted light is recorded by a self-developed hyperspectral module and then converted to broadband cavity-enhanced absorption spectrum (BBCEAS) via the Beer-Lambert law. An artificial neural network (ANN) is employed to predict pH values of the solution. With such a design, this system exhibits a wide detecting range of 2 M [H + ] − 2 M [OH – ] (corresponding to pH −0.3–14.3) with a response time of about 120 s. The system can achieve a higher detection accuracy with root mean square error (RMSE) of 0.073, as compared to 0.137 without the cavity enhancement. The system also possesses good properties of repeatability, long-term stability, ion resistance, and organic corrosion resistance. These excellent properties make the proposed system a promising candidate technology for harsh environments, such as seawater acidification warning, chemical plant sewage monitoring, and biological sample detection.
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