SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Yafang Wu,Yujia Tong,Li Zhang,Xiaohui Ju,Chenxuan Shao,Qi Zhang,Weixing Li,Weihong Xing
Ultrafiltration membrane for purification of proteins represents a significant research challenge. In order to improve the uniformity of membrane pores, carboxymethyl chitosan hydrogel (TB-g-MC) grafted by methacrylic anhydride and 2, 3, 4-trihydroxybenzaldehyd was proposed to construct polyethersulfone (PES) ultrafiltration membrane with crosslinked network. TB-g-MC with better hydrophilicity could effectively slow down the phase transition process, which led to more uniform membrane pores. The water flux of the PES/TB-g-MC membrane arrived at 880.2 L·m −2 ·h −1 , three times higher than that of the pristine membrane. The rejection of bovine serum albumin (BSA) was 99.5 % with the collagen permeability of 99.9 %, which showed that the developed membrane had narrow pore size distribution and better separation performance. Furthermore, it exhibited noteworthy anti-fouling characteristics with the flux recovery of 90 %. The proposed membrane with hydrogel network structure will be prospective for protein purification and production in biomedical field.
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Xinhu Gong,Ying Luo,Chaoqun Zhang,Jiating Xie,Yang Wang
Carboxymethyl chitosan (CA)/polyacrylamide (PAM) tough anti-freezing conductive (GCPC) hydrogels were fabricated via a simple one-pot method. The key innovation lies in strategic construction of interpenetrating network, reinforced by a synergistic interplay of hydrogen bonding, ionic coordination, and electrostatic interactions. Crucially, the CaCl 2 solution served a triple role: it dynamically cross-linked molecular chains to enhance mechanical toughness, acting as anti-freezing agent to suppress ice crystallization, enabling remarkable freezing resistance at ultra-low temperatures down to −50 °C, while simultaneously provides high conductivity for hydrogels as ionic electrolyte. These unique combinations endowed the GCPCs with remarkable strength (highest tensile strength of 1.24 MPa and highest compressive strength of 6.45 MPa), low hysteresis performance (6.02 % in tensile cycles and 22.09 % in compression cycles), excellent mechanical stability and durability (could withstand 1000 stretching and compression cycles without damage or relaxation) and high electrical conductivity (up to 7.20 S m −1 ). The GCPC could be assembled into a strain sensor and a temperature sensor, both of which delivered rapid, stable responses across extreme environments, highlighting their potential for multi-functional low-temperature wearable electronics and durable soft robotics. The present work is expected to provide a simple and efficient route for the preparation of multifunctional anti-freezing, tough and conductive hydrogels based on CA, and holds promise for a wide range of applications in soft sensor devices, suggesting new possibilities for technological progress within this domain.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Shiyu Liu,Naymul Karim,Ahmed K. Rashwan,Jiahong Xie,Wei Chen
Cyanidin-3-O-glucoside (C3G) is classified as an anthocyanin (ACN) and is recognized for its remarkable antioxidant properties. Yet, the inadequate physicochemical stability of C3G restricts its potential for various biological applications. Thus, in this study, carboxymethyl chitosan (CMC)-coated nanonutriosomes (NS) were synthesized as a novel carrier for encapsulating C3G (CMC-C3G-NS) to improve C3G stability. CMC-C3G-NS exhibited a diameter of less than 200 nm along with an encouraging encapsulation efficiency exceeding 90%. Notably, the formulated CMC-C3G-NS possessed better stability under various pH, ionic, and oxygen conditions, improved controlled release properties, and higher hepatocellular uptake than uncoated particles (C3G-NS), indicating a longer retention time of C3G in a physiological environment. Of utmost significance, CMC-C3G-NS demonstrated superior alleviating effects against palmitic acid (PA)-induced oxidative hepatic damage compared to C3G-NS. Our study provided promising nanocarriers with the potential to deliver hydrophilic ACNs and controlled release properties for PA-induced hepatotoxicity alleviation.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Xiaojie Zhang,Kangrui Ma,Liqin Zhao,Hong Peng,Yuefa Gong
To enhance the stability and adsorption performance of chitosan in Cr(VI)-contaminated acidic wastewater, a novel EDAC-modified-EDTA-crosslinked chitosan derivative (CSEC) was synthesized via a one-pot method with chitosan, 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDAC), and Na 2 EDTA as raw materials. To further improve the mechanical strength and separation performance of CSEC, a novel composite bead (CSEP) of CSEC and imidazolium-functionalized polysulfone (IMPSF) was prepared through a phase inversion method. The chemical composition and microstructure of CSEC and CSEP were characterized by FESEM, FTIR, NMR and XPS techniques. The maximum adsorption capacities of CSEC and CSEP for Cr(VI) were 145.96 and 135.82 mg g −1 at pH 3, respectively, and the equilibrium time for Cr(VI) adsorption by CSEC and CSEP was 5 min and 8 h, respectively. The adsorption process of Cr(VI) by both CSEC and CSEP was exothermic and spontaneous. Compared to CSEC, CSEP has significantly enhanced resistance to interference from coexisting anions. The removal mechanism of Cr(VI) by CSEP might involve redox reaction as well as electrostatic attraction between Cr(VI) oxyanions and various nitrogen cations, including protonated amino groups, guanidinium groups, protonated tertiary amine groups, and imidazolium cations. The CSEP beads have potential application value in the treatment of acidic wastewater containing Cr(VI).
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2024
·
IF 5.3
Jingxuan Lu,Juan Li,Shiyan Fu,Huanhuan Tan,Yuhui Hao
The combined chemotoxicity and radiotoxicity associated with uranium, utilized in nuclear industry and military applications, poses significant threats to human health. Among uranium pollutants, uranyl is particularly concerning due to its high absorptivity and potent nephrotoxicity in its + 6 valence state. Here, we have serendipitously found Na 2 SeO 3 facilitates the conversion of U(VI) to U(IV) precipitates. A novel approach involving nano-chitosan loaded internally with melatonin and externally modified with selenite (NPs Cs-Se/MEL) was introduced. This modification not only enhances the conversion of U(VI) to U(IV) but also preserves the spherical nanostructure and specific surface area, leading to increased adsorption of U(VI) compared to unmodified samples. Selenite modification improves lysosomal delivery in HEK-293 T cells and kidney distribution of the nanoparticles. Furthermore, NPs Cs-Se/MEL demonstrated a heightened uranium concentration in urine and exhibited remarkable efficiency in uranium removal, resulting in a reduction of uranium deposition in serum, kidneys, and femurs by up to 52.02 %, 46.79 %, and 71.04 %, respectively. Importantly, NPs Cs-Se/MEL can be excreted directly from the kidneys into urine when carrying uranium. The results presented a novel mechanism for uranium adsorption, making selenium-containing nano-materials attractive for uranium sequestration and detoxification.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Biophysics
·
2024
·
IF 2.8
Weng Shuni,Su Huanhuan,McClements David Julian,Wang Yuting,Li Teng,Fu Yuying
Curcumin (CUR) is found to exhibit a broad spectrum of potentially beneficial biological activities, which make it suitable as a nutraceutical ingredient in functional foods and supplements. But its application for this purpose is often limited by its low water-solubility, chemical stability, and bioavailability. In this study, acid-treated soybean protein isolate (SPI) and chitosan quaternary ammonium salt (CQAS) were used to assemble colloidal carriers for CUR to form soybean protein isolate-curcumin-chitosan quaternary ammonium salt nanoparticles (SPI-CUR-CQAS NPs). The nanoparticles formed were relatively small (< 400 nm), approximately spherical, and strongly positively charged (> 45 mV). Fluorescence spectroscopy demonstrated that the dominant interaction between CUR and SPI was hydrophobic attraction, while the dominant interaction between CQAS and SPI was electrostatic attraction. The encapsulation efficiency (95.4%) and loading capacity (14%) of the SPI-CUR-CQAS NPs (pH 1.5) were relatively high. The vitro digestion experiments showed that the CUR was progressively released from the nanoparticles under stomach, small intestine, and colon conditions. Moreover, the final bioavailability of the encapsulated CUR (63%) was much higher than for free CUR (18.5%). The SPI-CQAS NPs developed in this study can therefore be used as an effective delivery system for CUR in functional foods.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2024
·
IF 3.3
Ming Cheng,Ye Tao,Chunwei Wang,Ao Li
BACKGROUND Phytosterols (PS) have various beneficial effects on human health, especially the property of reducing blood cholesterol. However, the low solubility and bioaccessibility of PS have greatly limited their application in functional food ingredients. RESULTS To improve the bioaccessibility and stability of PS, chitosan-coated PS nanoparticles (CS-PNP) were successfully prepared by self-assembly. The properties of CS-PNP, including size, zeta potential, encapsulation efficiency (EE) and loading amount (LA) were characterised. The optimisation of CS concentration (0.4 mg mL −1 ) and pH (3.5) resulted in the formation of CS-PNP with an EE of over 90% and a particle size of 187.7 nm. Due to the special properties of CS chitosan, the interaction between CS and soybean protein isolate (SPI)/lecithin (SL) led to the formation of a soluble complex. CS-PNP exhibited good stability to temperature variations but was more sensitive to salt ions. During in vitro digestion, CS efficiently maintained the stability of nanoparticles against the hydrolysis of SPI by pepsin under acidic conditions. However, these nanoparticles tended to aggregate in a neutral intestinal environment. After 3 h of in vitro digestion, the bioaccessibility of PS increased from 18.2% of free PS to 63.5% of CS-PNP. CONCLUSION Overall, these results highlight the potential of chitosan-coated nanoparticles as effective carriers for the oral administration of PS. This multilayer construction may serve as a promising for applications in food products as delivery vehicles for nutraceuticals. © 2024 Society of Chemical Industry.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Lili Zhang,Yuxuan Xu,Tengyu Feng,Yu Zhang,Jiajun Sun,Xiaodong Wang,Chengying Bai,Xiaohong Zhang,Jun Shen
There is a growing demand for the development of epoxy resin modified with biomaterials, aiming to achieve high toughness. Herein, chitosan crosslinked epoxy resin (CE) was synthesized by diisocyanate as a bridge. With 4,4′-diamino-diphenylmethane (DDM) as the curing agent, thanks to the unique cross-linking structure of the CE resin and the presence of carbamate groups, the cured CE/DDM exhibited superior properties compared to commercially available epoxy resin (E51). The tensile strength of the cured CE-3/DDM reached 90.17 MPa, the elongation at break was 11.2 %, and the critical stress intensity factor (K IC ) measured 1.78 MPa m 1/2 . These values were 21.4 %, 151.6 %, and 81.6 % higher than those of the cured E51/DDM, respectively. It is worth noting that the addition of biomass material chitosan did not reduce the thermal stability of the resin. Additionally, the CE coatings on the metal substrate exhibited exceptional corrosion resistance , as evidenced by higher impedance values in electrochemical impedance spectroscopy (EIS) and polarization voltages in the Tafel curve compared to those of the E51 coating. This study opens up a novel approach to modifying epoxy resin with biomass materials with high toughness and corrosion resistance, without sacrificing other performance.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Xinyuan Zhang,Yongkang Lai,Liang Zhang,Zheng Chen,Jiulong Zhao,Shige Wang,Zhaoshen Li
Helicobacter pylori ( H. pylori ) is one of the major causes of gastrointestinal diseases, including gastric cancer. However, the acidic environment of the stomach and H. pylori resistance severely impair the antimicrobial efficacy of oral drugs. Here, a biocompatible chitosan-modified molybdenum selenide (MoSe 2 @CS) was designed for the simultaneous photothermal treatment of H. pylori infection and gastric cancer. MoSe 2 @CS showed a photothermal conversion efficiency was as high as 45.7 %. In the H. pylori -infected mice model, MoSe 2 @CS displayed a high bacteriostasis ratio of 99.9 % upon near-infrared irradiation. The antimicrobial functionality was also proved by transcriptomic sequencing study, which showed that MoSe 2 @CS combined with NIR laser irradiation modulated the gene expression of a variety of H. pylori bioprocesses, including cell proliferation and inflammation-related pathways. Further gut flora analysis results indicated that MoSe 2 @CS mediated PTT of H. pylori did not affect the homeostasis of gut flora, which highlights its advantages over traditional antibiotic therapy. In addition, MoSe 2 @CS exhibited a good photothermal ablation effect and significantly inhibited gastric tumor growth in vitro and in vivo . The comprehensive application of MoSe 2 @CS in the PTT of H. pylori infection and gastric cancer provides a new avenue for the clinical treatment of H. pylori infection and related diseases.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Yanke Lu,Pengfei Li,Liuliu Cheng,Lianfeng Ai,Jiankun Cao,Hongyuan Yan
Halogenated carbazoles (HCZs) are prevalent in sediments, pose a risk of entering the human food chain via lotus roots. However, starch, protein, and fat in lotus root complicate determination of HCZs in this food. This study introduces a straightforward, sensitive, and rapid method for detecting four HCZs in lotus roots. The method employs a conical monolithic extraction column (MEC) containing chitosan-glutaraldehyde-graphene oxide aerogel (CS-GA-GOA) material. The CS-GA-GOA − MEC successfully achieved extraction of four HCZs in spiked lotus root samples in under 5 min. The method was rapid (15 min) compared with alternatives, as well as being sensitivity (0.08–0.27 ng/g), accurate (89.7 %–104.2 %), and more cost-effective. This work contributes to the exploration of advanced adsorbents but also broadens the applicability of graphene oxide aerogels in extraction processes, paving the way for the development of rapid and accurate analytical methods for HCZs in foods.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Yonghe Wang,Yulan Wang,Jing Guo,Fucheng Guan,Xinbin Ji,Minghan Li,Qiang Yang,Jianbin Sun,Shi Feng,Jing Tao
In scenarios of arterial bleeding, common hemostatic agents or dressings often fail due to the loss of active components, high blood loss, and high bleeding pressure. To overcome these challenges, we have developed a novel bio-based hemostatic membrane capable of rapid hemostasis under high pressure. A biomass hemostatic membrane with rapid hemostatic function under high pressure was prepared based on polyelectrolyte electrostatic self-assembly and a “concrete reinforcement” strategy. Hyaluronic acid (HA) was sprayed by airflow to form CSH hydrogel bubbles in the Chitosan (CS) solution. Then CSH hydrogel bubbles were deposited onto cellulose nonwoven (CN) by vacuum filtration, and the CSH composite membrane was obtained after drying. The hemostatic effect of several CSH composite membrane was evaluated in the analysis of venous bleeding in vitro (1.5 KPa). Among them, CS 1 HA 0.4 @CN had a small amount of blood loss, but the amount of blood loss was only about 0.29 g, the other samples did not show any blood loss. In vitro arterial bleeding analysis, CS 1 HA 0.2 @CN showed significant hemostasis in just 20 s and successfully stabilized the blood flow at 0.002 mL/s within 40 s. Despite being subjected to a high pressure of 96.235 KPa, the CSH composite membrane effectively maintains its hemostatic capability. Consequently, it is viewed as a promising solution for emergency arterial hemostasis.
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Min Wang,Yajie Yue,Xiaoning Wei,Jinyuan Zhang,Xinyuan Bi,Liyan Jia,Xu Jing
Strobilurin fungicides are widely used pesticides globally; however, their residues pose a risk to environmental safety and human health. This study aimed to develop a rapid and green method for detecting strobilurin fungicide residues. Chitosan and magnetic deep eutectic solvent-based emulsive liquid-liquid microextraction method combined with high-performance liquid chromatography was developed to determine strobilurin fungicides in water samples. A high-concentration oil-in-water emulsion containing chitosan solution and extractant was formulated, followed by the addition of samples to form a low-concentration oil-in-water emulsion to complete the rapid pretreatment, therefore solving the limitation of emulsive liquid-liquid microextraction in the inapplicability of extractants. Traditional microextraction required toxic solvents and complex equipment to facilitate the dispersion of extractants, whereas this study used only chitosan solution to aid in emulsifying extractants. The new green magnetic deep eutectic solvent synthesized by tetraoctylammonium bromide, ferric chloride, and oleic acid was used instead of toxic extractants. An external magnetic field assisted the extractant phase collection, which shortened the separation time. Under optimal conditions, the recovery of this method was 83.3 %–107.4 %, the limit of detection was 0.0010–0.0021 mg L −1 , and the coefficient of determination was >0.994. This rapid, green, simple, and efficient chitosan-based microextraction method could detect strobilurin fungicide residues in water samples because of its enhanced compatibility with hydrophobicity extractants.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）