STEROIDS
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2025
·
IF 2.3
Jiao Yao,Lin-Hong Lu,Sheng-Yun Wei,Ting Wu,Wei Yang,Wei-Hua Wang,Fa-Zhong Yang,Yun-Xian Li,Ping Zhao,Guo-Lei Zhu
Three ergostane-type steroids, including one rare C-29 methylated xylarstane A ( 1 ) and one C22,23-epoxied xylarstane B ( 2 ), along with one reported compound 3 , were isolated from the endophytic fungus Xylaria sp. VDL4 harbored within the medicinal plant Vaccinium dunalianum . The structures of novel compounds were elucidated through comprehensive spectroscopic analysis, DP4 + -validated theoretical 13 C nuclear magnetic resonance (NMR) calculations, and electronic circular dichroism (ECD) calculations. The antifungal activities of the isolates against four phytopathogens were assessed in vitro. Compounds 1 and 3 exerted significant inhibition against Alternaria solani and Botrytis cinerea respectively, both with minimal inhibitory concentration (MIC) of 12.5 μg/mL, comparing with the positive control (Carbendazim and Thiabendazole, MICs = 12.5–25.0 μg/mL)
研究领域:
天然产物化学
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微生物生物技术
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植物病理学
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药用真菌学
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结构鉴定
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2018
·
IF 3.974
Fei Li,Lin Di,Yuxin Liu,Qiuyun Xiao,Xiaoyu Zhang,Fuying Ma,Hongbo Yu
Although ascomycetes occupy a vaster niche in soil than the well-studied basidiomycetes , they have received limited attention in studies related to bioremediation. In this study, the degradation of carbaryl by Xylaria sp. was studied in different culture conditions and its possible metabolic pathway was elucidated. In liquid culture, 99% of the added carbaryl was eliminated when cytochrome P450 (CYP450) was active, which was similar to the degradation rate of Pleurotus ostreatus , a fungus with strong bioremediation ability. Mn 2+ is beneficial to the degradation of carbaryl. Compared to the 72.17% degradation rate in sterile soil, 59.0% carbaryl was eliminated in non-sterile soil, which suggested that Xylaria sp. BNL1 can resist microorganismal infection. Furthermore, the intracellular fractions containing laccase, CYP450, and carbaryl esterase efficiently degraded carbaryl. The presence of carbaryl metabolites suggested that Xylaria sp. BNL1 initiated its attack on carbaryl via carbaryl esterase to release α-naphthol, which was further degraded to 1,4-naphthoquinone and benzoic acid by CYP450 and laccase. Thus, our study highlights the potential of using Xylaria sp. for bioremediation.
研究领域:
环境科学
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微生物学
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PubMed
高影响因子
BIOCHEMICAL SYSTEMATICS AND ECOLOGY
·
2023
·
IF 1.6
Di Zhang,Jinyang Zheng,Mingyi Chu,Yao Feng,Huifang Li
Eight compounds, including three mevalonic acid derivatives ( 1 - 3 ), one flavonoid ( 4 ), and four substituted benzene-ring derivatives ( 5 – 8 ), were isolated from the fermentation of an endophytic fungus Cladosporium sp. ZLM-6 derived from Suaeda salsa (Linn.) Pall. Compounds 1 – 4 and 6 – 8 were identified from the genus Cladosporium for the first time. Their antimicrobial activities in vitro showed that compound 4 possessed good inhibitory activity against both Pseudomonas aeruginosa and Escherichia coli , with MIC values of 5 and 10 μg/mL, respectively. Additionally, compound 4 showed moderate inhibitory activity against four different vibrios with MIC values of 35 μg/mL. The chemical diversity of the genus Cladosporium was further expanded, and moreover the significance of all compounds in chemotaxonomy was also discussed briefly in this work.
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2019
·Vol. 20
·期 18
·4376
·
IF 4.183
Yi Zhang,Kedong Xu,Deshui Yu,Zhihui Liu,Chunfeng Peng,Xiaoli Li,Ju Zhang,Yinghui Dong,Yazhen Zhang,Pan Tian,Tiancai Guo,Chengwei Li
Effector proteins secreted by plant pathogens play important roles in promoting colonization.Blumeriaeffector candidate (BEC) 1019, a highly conserved metalloprotease ofBlumeria graminisf. sp.hordei(Bgh), is essential for fungal haustorium formation, and silencingBEC1019significantly reducesBghvirulence. In this study, we found thatBEC1019homologs inB. graminisf. sp.tritici(Bgt) andGaeumannomyces graminisvar.tritici(Ggt) have complete sequence identity with those inBgh, prompting us to investigate their functions. Transcript levels ofBEC1019were abundantly induced concomitant with haustorium formation inBgtand necrosis development inGgt-infected plants.BEC1019overexpression considerably increased wheat susceptibility toBgtandGgt, whereas silencing this gene using host-induced gene silencing significantly enhanced wheat resistance toBgtandGgt, which was associated with hydrogen peroxide accumulation, cell death, and pathogenesis-related gene expression. Additionally, we found that the full and partial sequences ofBEC1019can trigger cell death inNicotiana benthamianaleaves. These results indicate thatBgtandGgtcan utilizeBEC1019as a virulence effector to promote plant colonization, and thus these genes represent promising new targets in breeding wheat cultivars with broad-spectrum resistance.Keywords:effector protein;Blumeria graminisf. sp.tritici;Gaeumannomyces graminisvar.tritici;haustorium formation;necrosis development;cell death
研究领域:
植物病理学
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分子生物学
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植物育种
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PubMed
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NATURAL PRODUCT RESEARCH
·
2024
·
IF 1.9
Di Zhang,Yao Feng,Mingyi Chu,Yujiang Dai,Lu Jiang,Huifang Li
The infection of vibrio is an important cause of huge economic losses in aquaculture industry. At present, antibiotics are mainly used to prevent and reduce the infection of the vibrio, which has accelerated the emergence of multi-drug-resistant strains. New generation alternative anti-vibrio drugs were in urgent to solve this problem. In this study, six compounds (1–6) were isolated from the Streptomyces sp. ZZ741A, a marine-derived Streptomyces variant, including one new compound, 2-carbamoylphenyl isobutyrate (1), five known ones, nocardamine (2), dehydroxynocardamine (3), phenylacetic acid (4), thiophenol (5) and 2,3-dihydroxybenzoic acid (6). The anti-vibriosis assay showed that compounds 2 and 3 had specific inhibition activity against Vibrio vulnificus, Vibrio alginolyticus, and Vibrio parahaemolyticus with the MIC values ranging from 8 to 128 μg/mL. The molecular docking study of their possible mechanism of anti-vibriosis activity showed that the activity might come from the inhibition of Outer membrane protein U (OmpU).
研究领域:
微生物学
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水产养殖学
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药理学
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PubMed
高影响因子
TETRAHEDRON
·
2018
·
IF 2.377
Mengxuan Chen,Weiyun Chai,Rongyao Zhu,Tengfei Song,Zhizhen Zhang,Xiao-Yuan Lian
Secondary metabolites from marine-associated actinomycetes are important source for the discovery of novel bioactive compounds. In this study, an actinomycete Streptomyces sp. ZZ446 was isolated from coastal soils and different media were used to culture this isolated marine actinomycete. It has been found that this actinomycete in the liquid medium of 2216 E with sea salt produced five new compounds of streptopyrazinones A−D ( 1 – 4 ) and N -acetyl- l -isoleucine- l -leucinamide ( 5 ) as well as six known diketopiperazines ( 6 – 11 ) and one alkaloid ( 12 ). Structures of the new compounds were determined by extensive NMR analyses, HRESIMS data, electronic circular dichroism (ECD) calculation, chemical degradation , Marfey's method, and X-ray diffraction analysis. This type of streptopyrazinones A−D ( 1 – 4 ) is rarely found in the natural resources. New compounds 1 – 5 showed activity in inhibiting the growth of Candida albicans and methicillin-resistant Staphylococcus aureus .
研究领域:
微生物学
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药物化学
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天然产物研究
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高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2018
·Vol. 102
·期 4
·1997-2006
·
IF 3.34
Wang Shizong,Yin Yanan,Wang Jianlong
A novel strain capable of degrading triclosan was isolated from the acclimated activated sludge and identified to be Dyella sp. WW1 based on 16S rDNA analysis. The effect of initial concentration of triclosan (0.2, 1, 5, and 10 mg/L), temperature (15, 25, and 35 °C), pH (5, 7, and 9), and additional carbon source on the degradation of triclosan was investigated in a mineral medium. The results showed that Dyella sp. WW1 can use triclosan as sole carbon source and degrade it when initial triclosan concentration was in the range of 0.2–10 mg/L. The optimal condition for Dyella sp. WW1 to degrade triclosan was 15 °C and pH 7. TOC removal efficiency was more than 90%. Dyella sp. WW1 can degrade 3,5-dichloro-4-hydrobenzoic via co-metabolism in the presence of triclosan, but cannot degrade trimethoprim, sulfamethoxazole, carbamazepine, and diclofenac. In the presence of glucose, Dyella sp. WW1 firstly utilized glucose to synthesize the biomass and then degraded triclosan. When triclosan concentration decreased to an extent (1.2 mg/L in this study), Dyella sp. WW1 started to use glucose again. The wastewater components did not significantly affect the activity of Dyella sp. WW1 to degrade triclosan. During the biodegradation process, six metabolite products were identified. Based on the metabolites, two degradation pathways were tentatively proposed. In summary, Dyella sp. WW1 could be used for degrading triclosan in the real wastewater.
研究领域:
微生物学
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环境科学
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CATALYSIS LETTERS
·
2020
·Vol. 150
·期 8
·2415-2426
·
IF 2.482
Tang Kexin,Yi Yunfei,Gao Zhen,Jia Honghua,Li Yan,Cao Fei,Zhou Hua,Jiang Min,Wei Ping
Abstract In present study, we have mined a transaminase (TA) from Rhizobium sp. from the pool of fully sequenced genomes by using an ω-TA sequence from Vibrio fluvialis JS17 as a template in a BLASTP search. The protein sequence of the TA from Rhizobium sp. exhibits 53% sequence identity to that from V. fluvialis . The TA with S -selectivity showed close evolutionary relationship with a pyruvate transaminase from Alcaligenes denitrificans Y2k-2 and an S -selective aminotransferase from Sphaerobacter thermophilus . The gene of the ω-TA was inserted into pET-28a and functionally expressed in E. coli BL21. Results showed that the recombinant ω-TA has a specific activity of 7.46 U/mg at pH 8.0, 30 ℃. The substrate specificity test found the ω-TA presented significant reactivity toward aromatic amino donors and amino acceptors containing aldehydes. More importantly, the ω-TA also exhibited a good affinity towards some cyclic substrates. The homology model of the ω-TA was built by Discovery Studio and docking was performed to describe the relative activity towards some substrates. Graphic Abstract
研究领域:
生物化学
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分子生物学
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微生物学
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生物技术
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高影响因子
Insects
·
2025
·Vol. 17
·期 1
·3
·
IF 2.9
Zhengyan Wang,Qiong Luo,Yifan Liu,Tianwei Ye,Yujia Zhang,Wei Xu
In agricultural production, the extensive use of insecticides has led to the persistent presence of residues such as deltamethrin in the environment, posing threats to ecosystems and human health. Utilizing microbes for environmental bioremediation presents a feasible approach. Studies have found that insects with strong insecticide tolerance often harbor gut bacteria capable of degrading insecticides. These gut bacteria are becoming an excellent reservoir of insecticide-degrading bacteria with bioremediation potential. However, in practical applications, the activity of free bacterial cells is easily affected by environmental factors, limiting the stability of their remediation efficacy. This study focused on a Microbacterium sp. strain isolated from insects. By optimizing culture conditions and the sodium alginate immobilization process, the remediation efficiency of the immobilized strain in contaminated soil and water was investigated. The results demonstrated that the immobilized strain significantly outperformed free bacteria in deltamethrin degradation performance in both contaminated soil and water, while also exhibiting good stability and reusability. This research provides an effective method for the remediation of deltamethrin-contaminated environments. Extensive application of deltamethrin on agricultural products results in serious contamination of the environment. Its negative impact on environmental and public health necessitates the development of environmental remediation technologies. Detailed investigations of microbial degradation of deltamethrin may be useful for the development of bioremediation strategies. In this study, the deltamethrin removal capability of a bacterial strain, Microbacterium sp., previously isolated from the gut of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) was first investigated. When 3 mL of the bacterial solution (OD 600 = 1) was inoculated into 97 mL of MS media containing 200 μg/mL deltamethrin, it could remove 45.7% deltamethrin after 64 h of incubation. This strain grew fastest in LB media with an inoculum volume of 3% in pH 7 at 175 rpm, 25 °C. To enhance its environmental tolerance, this strain was immobilized with sodium alginate. Microbacterium sp.-containing calcium alginate microspheres (CAMs) exhibited an enhanced deltamethrin removal capability compared to free bacteria, and CAMs generated by immobilization with 2% sodium alginate and 3% CaCl 2 cross-linking for 4 h possessed the maximum deltamethrin removal capability. The ultrastructure of Microbacterium sp.-containing CAMs prepared under optimal conditions was a three-dimensional mesh structure with pores and dense features, and the bacteria grew well in the immobilized carrier. After being reused five times, the deltamethrin removal rate of immobilized Microbacterium sp. still reached over 50%. When Microbacterium sp. was inoculated into deltamethrin-contaminated water or soil for 48 h, the deltamethrin removal rate of immobilized bacteria was 1.4 times higher than that of free bacteria. These results suggest that Microbacterium sp.-containing CAMs possess an excellent deltamethrin removal capability and good reusability, showing great potential for the remediation of deltamethrin-contaminated environments.
研究领域:
环境微生物学
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生物修复技术
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应用微生物学
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农药降解
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微生物发酵
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JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Pan Yang,Jinlan Tang,Zhuorui Ding,Yiqun Chen,Zizheng Liu,Jianping Yuan,Yuhua Zhu,Pengchao Xie
Carbocatalysts, widely regarded as eco-friendly catalysts in the field of peroxymonosulfate (PMS) activation for pollutant removal, exhibit significant variations in performance depending on the type of carbon atom hybridization. Notably, the novel graphdiyne (GDY), characterized by its sp-hybridized carbon (sp-C), has recently garnered significant attention. However, the precise mechanistic role of sp-C on PMS activation remains unclear. Herein, we elucidate the role of sp-C on PMS activation and the corresponding mechanism behind enhanced phenolic pollutant degradation over the GDY catalyst. GDY demonstrates exceptional phenolic removal efficiency (97 %), which far exceeds that of traditional sp 2 -hybridized graphene (2 %). The GDY facilitates pollutant oxidation via a catalyst-mediated electron transfer mechanism. The sp-C provides additional sites for PMS adsorption and donates significantly more electrons from GDY to PMS (0.51 e more than graphene), effectively facilitating PMS activation, intermediate species conversion, and reaction kinetics during phenolic pollutant degradation. By integrating the monolithic GDY catalyst into a flow-through device for continuous phenolic pollutant removal, long-lasting phenolic removal was maintained for over 80 hours, with a removal rate exceeding 85 %. This work highlights that sp-C in GDY effectively enhances PMS activation, providing a pathway for efficient organic pollutant degradation in advanced oxidation processes.
研究领域:
环境科学
·
催化化学
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PubMed
高影响因子
Foods
·
2021
·Vol. 10
·期 10
·2453
·
IF 4.35
Jingfei Hu,Xueqing Tian,Tong Wei,Hangjie Wu,Jing Lu,Mingsheng Lyu,Shujun Wang
Helicobacter pyloriis a cause of gastric cancer. We extracted the exopolysaccharide (EPS) ofLactobacillus plajomiPW-7 for antibacterial activity versusH. pylori, elucidating its biological activity and structural characteristics. The minimum inhibitory concentration (MIC) of EPS againstH. pyloriwas 50 mg/mL. Disruption of the cell membranes of pathogenic bacteria by EPS was indicated via the antibacterial mechanism test and confirmed through electron microscopy. EPS also has antioxidant capacity. The IC50of EPS for 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide anions, and hydroxyl radicals were 300 μg/mL, 180 μg/mL, and 10 mg/mL, respectively. The reducing power of EPS was 2 mg/mL, equivalent to 20 μg/mL of ascorbic acid. EPS is a heteropolysaccharide comprising six monosaccharides, with an approximate molecular weight of 2.33 × 104Da. Xylose had a significant effect onH. pylori. EPS fromL. plajomiPW-7 showed potential as an antibacterial compound and antioxidant, laying a foundation for the development of EPS-based foods.Keywords:Helicobacter pylori;Lactobacillus plajomi;exopolysaccharide;antibacterial activity;antioxidant activity
研究领域:
微生物学
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食品科学
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医学
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PubMed
高影响因子
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
·
2017
·Vol. 33
·期 5
·1-8
·
IF 1.658
Pan Lan-jia,Tang Xiao-da,Li Chun-xing,Yu Guang-wei,Wang Yin
Sulfamethazine (SM2) is an antimicrobial drug that is frequently detected in manure compost, is difficult to degrade at high temperatures and is potentially threatening to the environment. In this study, a thermophilic bacterium was isolated from the activated sludge of an antibiotics pharmaceutical factory; this bacterium has the ability to degrade SM2 at 70 °C, which is higher than the traditional manure composting temperature. The strain S-07 is closely related to Geobacillus thermoleovorans based on its 16S rRNA gene sequence. The optimal conditions for the degradation of SM2 are 70 °C, pH 6.0, 50 rpm rotation speed and 50 mL of culture volume. More than 95% of the SM2 contained in media was removed via co-metabolism within 24 h, which was a much higher percentage than that of the type strain of G. thermoleovorans . The supernatant from the S-07 culture grown in SM2-containing media showed slightly attenuated antibacterial activity. In addition, strain S-07 was able to degrade other sulfonamides, including sulfadiazine, sulfamethoxazole and sulfamerazine. These results imply that strain S-07 might be a new auxiliary bacterial resource for the biodegradation of sulfonamide residue in manure composting.
研究领域:
环境微生物学
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生物修复
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药学
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PubMed
高影响因子
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