Scientific Reports
·
2025
·Vol. 15
·期 1
·1-11
·
IF 3.9
Wu Chunfei,Bi Chuanlin,Kim Geun-soo,Yang Zizhen,Li Shuao,Dai Tong,Wu Xiaoyu,Tan Jiaojiao,He Ningning,Li Shangyong
Tofacitinib (Tof), a commercially available pan-Janus kinases inhibitor, is approved for the treatment of moderate to severe ulcerative colitis. However, its clinical application is limited due to dose-dependent systemic side effects. The present study aims to develop an efficient oral colon-targeted drug delivery systems using prebiotic pectin (Pcn) and chitosan (Csn) polysaccharides as a shell, with Tof loaded into a Bovine Serum Albumin (BSA) core, and improving it with chondroitin sulfate (Chs), thu s constructing Tof@BSA-Chs-CP nanoparticles (NPs). Our results suggest that th e pH-sensitive characteristics of the Pcn/Csn shell contribute to its capacity for attenuating absorption and systemic diffusion in the gastrointestinal tract, and exhibiting targeted localization at inflamed colonic sites in mice. Additionally, the gut microbiota-secreted polysaccharide-degrading enzyme acts as the triggering agent for Pcn/Csn shell degradation. In mice colitis models, we demonstrated that oral administration of Tof@BSA-Chs-CP NPs effectively ameliorated colitis and expedited its resolution by modulating the expression of pro-inflammatory cytokines and immune regulatory factors. Collectively, our synthetic NPs demonstrate the promising potential of Tof for the therapy of UC.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2024
·
IF 5.7
Jinyan Gu,Lianzhu Lin,Mouming Zhao
Due to the difficulty in obtaining highly branched rhamnogalacturonan-I (RG-I) type pectin, the relationship between the extent of RG-I branching (EB) of pectin and prebiotic/immunomodulatory activity has not been systematically investigated. Moreover, it is only possible to establish a structure–activity relationship using pectin that is highly purified and accurately characterized. In this study, a homogeneous highly branched RG-I type pectin (LBP-P4, a final product) with dual proliferative effects on Bifidobacterium and macrophage was effectively purified for the first time using enzyme hydrolysis combined with ultrafiltration. The RG-I content and EB of LBP-P4 reached 97.32 and 77.12, respectively. Its two branches were composed of arabinan and arabinogalactan-II, containing → 5)-Araf-(1→, →3)-Araf-(1→, →3,6)-Galp-(1→ and →6)-Galp-(1→ residues). The structure–activity relationship analysis indicated that strong prebiotic/immunomodulatory activity of LBP-P4 was depended on its high EB, which was further confirmed by the molecular docking simulation between low/high branched pectin with β-1,6-galactosidase, α-l-arabinanase, and Toll-like receptor 4 (TLR4).
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CERAMICS INTERNATIONAL
·
2025
·
IF 5.6
Shuang Qiu,Xuanwen Xu,Zehua Feng,Lu Li,Xiaoqian Wang,Kai Zheng,Yan Xu
Periodontitis, an inflammatory disease primarily driven by bacterial infection, often leads to the destruction of alveolar bone, presenting a major clinical challenge. To address this, the development of biomaterials that not only inhibit bacterial activity but also stimulate osteogenesis has garnered significant attention. In this study, novel nanocomposite pectin hydrogel microspheres were developed to exhibit both antibacterial and pro-osteogenic properties, as a potential solution for bone tissue regeneration. These microspheres were fabricated by integrating SiO 2 -CaO mesoporous bioactive glass nanoparticles (MBGNs) into pectin hydrogels, followed by cross-linking with either Ca ions or Zn ions. The physicochemical properties, osteogenic potential, and antibacterial efficacy of the resulting microspheres were assessed. The results revealed that the Zn-crosslinked pectin hydrogel microspheres, incorporating MBGNs, exhibited a porous structure with enhanced swelling behavior and slower degradation compared to their Ca-crosslinked counterparts. Notably, these Zn-crosslinked microspheres demonstrated superior antibacterial activity against periodontitis-associated pathogens and significantly promoted osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (hBMSCs). These findings underscore the potential of Zn-crosslinked pectin hydrogel microspheres with MBGNs as a promising therapeutic strategy for enhancing bone tissue regeneration in the context of periodontitis.
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Food Chemistry-X
·
2025
·
IF 8.2
Keyu Du,Jie Shen,Siyao Zhong,Hefei Wang,Yulou Qiu,Xuping Shentu,Xiaoping Yu,Zihong Ye,Haizhi Huang
Nitrofurazone and its metabolite semicarbazide (SEM) residues present in food products pose substantial health hazards to humans. Herein, the colorimetric–fluorescent lateral flow immunoassay (LFIA) for 4[(4-carboxyphenyl)-methylene]-hydrazinecarboxamide (CPSEM) detection was developed using PCN-222@Pectin as a signal tag. Herein, the PCN-222 surface was subsequently modified with pectin to enhance its aqueous solubility and stability. The PCN-222@Pectin-LFIA system exhibited a broad linear range from 0.025 ng/mL to 100 ng/mL. The low limits of detection for the colorimetric and fluorescence-based detection methods were 0.1 and 0.05 ng/mL, respectively. Notably, these values represent a 10- and 20-fold higher sensitivity than that of the traditional colloidal gold nanoparticle-based LFIA. This method was successfully used to detect CPSEM in actual samples, with recovery rates of 89.34 %–120.80 %. This approach fulfills the need for on-site preliminary qualitative assessment during detection and permits subsequent accurate quantitative analysis.
研究领域:
食品安全
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分析化学
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生物传感器
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环境监测
·
纳米材料
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Biomaterials Advances
·
2025
·
IF 6
Junling Wang,Jingjing Zhu,Yanfang Lu,Aihong Lou,Zhe Cui,Yong Wang,Xianling Wang,Jianglei Qin
Natural polymer-based biodegradable hydrogels can be used to promote the wound healing due to their excellent biocompatibility, tissue adhesion and wound adaptability. In this research, a sprayable hydrogel with good antioxidant, skin adhesion and self-healing property was designed from hydrazide and dopamine functionalized carboxymethyl cellulose (CMC-HD) with oxidized pectin (OPec) cross-linking. The dopamine moiety enhanced antioxidant property of the hydrogel and the MXene nanosheets were composited to take the advantage of the photothermal stimulated antibacterial activit y . The coagulation drug Tranexamic acid (TXA) was loaded and enhance the hemostatic performance of the hydrogel in tail amputation and liver hemorrhage models. In vivo wound repairing experiment in full-thickness skin wound model proved the MXene@TXA/Gel promoted the wound repairing rate under NIR stimulus and this composite hydrogel shows great potential as wound dressing.
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FOOD HYDROCOLLOIDS
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2024
·
IF 11
Yajuan Qin,Yutong Zhang,Xiaoai Chen,Fei Xu,Kexue Zhu,Ping Wang,Yanjun Zhang
In this study, the effect and mechanism of pectin on the inhibition of in vitro starch digestion by a mixture of flavanols (rutin, quercetin, and kaempferol [R–Q–K]) were investigated, mainly from an enzymatic point of view. Results showed that the association of pectin and R–Q–K decreased the hydrolysis rate, rapidly digestible starch content, estimated glycemic index ( eGI ) values and hydrolysis index ( HI ) values, and increased the resistant starch content of different crystalline starches. Among them, cassava starch exhibited a significant decrease in terms of HI and eGI values (32.81% ± 0.65% and 10.57% ± 0.37%, respectively). Meanwhile, the lowest rate of decrease in terms of HI and eGI values was found for breadfruit starch (22.97% ± 0.39% and 7.23% ± 0.62%, respectively). The inhibitory effect of R–Q–K on α-glucosidase was attenuated by low concentrations of pectin, which was correlated to the non-covalent binding among pectin, α-glucosidase, quercetin, and kaempferol. The hydrogen bonding between pectin–R–Q–K and starch may be responsible for the ability of low concentrations of pectin and R–Q–K to synergistically delay starch digestion. Higher concentrations of pectin were noted to have the opposite trend, which was attributed to forming the binary complex of pectin–α-glucosidase. The inhibitory affinity and quenching affinity of R–Q–K for α-glucosidase were enhanced by low concentrations of pectin and attenuated by high concentrations of pectin. This study aims to provide theoretical support for the development of pectin and flavonoids in starch-based healthy foods.
研究领域:
食品科学
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营养学
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生物化学
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LWT-FOOD SCIENCE AND TECHNOLOGY
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2024
·
IF 6
Dan Wang,Baiqian Wang,Xuejiao Jin,Yuanyuan Peng,Jinyan Zhao,Meng Zhang,Yunlu Wei,Zhijian Long,Qiyang Chen
This study aimed to optimize the extraction of feijoa pulp pectin and discuss the impact of different extraction methods on the physicochemical and functional properties of pectin. Under the optimum conditions, the pectin yields of microwave-enzyme-assisted extraction (PMEAE, 13.31%) and enzyme-assisted extraction (PEAE, 9.26%) were significantly higher than that of conventional heating extraction (PCHE, 7.64%). Compared to PCHE, PMEAE and PEAE exhibited higher degree of esterification of 68.43% and 67.89%, respectively, coupled with more highly branched side chains, which was confirmed by Fourier-transform infrared (FTIR) analysis. Differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) analysis showed that PMEAE and PEAE exhibited superior thermal stability. PEAE and PMEAE owned higher viscosity and superior antioxidant biological activity than PCHE. This study suggested that feijoa pectin by microwave enzyme-assisted or enzyme-assisted extraction contained more branched side chains and had better physicochemical and functional properties.
研究领域:
食品科学
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化学工程
·
生物技术
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2022
·
IF 8.025
Bihua Shen,Qiong Zhang,Chenmin Zheng,Yufan Huang,Guoguang Zhang,Peng Fei,Shirong Hu
In this study, an interpenetrating double-network hydrogel (LMP/AA/PVAH) was prepared based on low methoxy pectin (LMP), acrylic acid (AA) and polyvinyl alcohol (PVA). The first rigid network of chemical crosslinking was constructed via free radical polymerization of LMP and AA , and the second of ductile physical crosslinking network was constructed via cyclic freeze-thaw of PVA. The first cycle hardness and elasticity of the LMP/AA/PVAH significantly increased from 13.08 N and 0 to 24.28 N and 0.79, respectively, when the second network structure was constructed in the hydrogel by PVA. Besides, the PVA network might enhance the ductile and limit the swelling of hydrogel. In addition, the adsorption properties of LMP/AA/PVAH were evaluated by adsorption of methylene blue (MB). The adsorption behavior of MB by LMP/AA/PVAH conformed to the pseudo-second-order kinetic model. Besides, after 4 cycles of adsorption, there was no significant difference in adsorption capacity of LMP/AA/PVAH. The results showed that LMP/AA/PVAH had good reusability.
研究领域:
材料科学
·
环境科学
·
化学工程
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JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
·
2017
·Vol. 17
·期 1
·681-689
·
IF 1.483
Cui Si-Si,Sun Xue,Yao Bing,Peng Xiao-Xia,Zhang Xin-Tong,Zhou Yi-Fa,Hu Jun-Li,Liu Yi-Chun
Pectin, a natural plant polysaccharide, holds great potential for biomedicine. Developing low molecular weight (Mw) pectin-based nanofibers is desirable for biomedical applications in which fast degradation and elimination of polymer from the body are required. Here, we report the first
work on fabricating low Mw pectin-based nanofibers through electrospinning, among which the content of carrier polymer, poly(ethylene oxide) (PEO), can be minimized to 10%. Surfactant (Triton X-100), high polymer concentration and cosolvent were essential to electrospin bead-free nanofibers
at low PEO content. The size of pectin nanofibers was dependent on polymer concentration and cosolvent. The presence of cosolvent inhibited the crystallization of PEO, but enhanced the crystallization of pectin. Meanwhile, glycerol as cosolvent could lead to phase separation of polymers. This
work provides a new prospective for the fabrication of low Mw pectin nanofibers suitable for in vivo applications with the demand of fast degradation.
研究领域:
生物材料学
·
生物医药工程
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CARBOHYDRATE POLYMERS
·
2026
·
IF 12.5
Junhao Ye,Ruiquan Hou,Kang Xie,Guangmin Ou,Zhixiang Zhang,Dongmei Cheng
The plant-derived photoactivated fungicide α-Terthienyl (α-T) exerts antimicrobial effects through light-induced reactive oxygen species generation. However, its agricultural application has been constrained by poor targeting capability and low photostability. To address these challenges, we developed a dual-responsive nanoparticle delivery system (α-T NPs) based on a pectin-chitosan polymer network that specifically recognizes the acidic and pectinase-rich microenvironment created by Botrytis cinerea hyphae secretions. Controlled release studies demonstrated that both acidic conditions (pH 5.0) and pectinase exposure substantially promoted α-T release, achieving cumulative release rates of 71.73% and 74.49% respectively over 60 h. The nanoparticle system showed no adverse effects on healthy tomato leaf growth while forming a protective barrier that effectively suppressed fungal infection. Targeted delivery significantly enhanced fungicidal efficacy, with α-T NPs exhibiting an EC₅₀ of 0.419 mg/L compared to 1.943 mg/L for α-T (technical material, TC). Confocal microscopy and quantitative analysis confirmed the enhanced targeting mechanism, revealing substantially stronger fluorescence intensity and higher α-T accumulation specifically on treated fungal hyphae. Additionally, α-T NPs significantly improved photostability, extending the half-life by approximately 1.93-fold under continuous illumination compared to α-T (TC). This work establishes an innovative “pathogen-activated” delivery platform with promising applications in sustainable crop protection.
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CARBOHYDRATE POLYMERS
·
2026
·
IF 13.2
Wenxin Ma,Qian Li,Tongcheng Xu,Fangling Du,Baorui Li,Yanqi Li,Aizhen Zong
Quercetin is a bioactive flavonoid with diverse biological activities, but its poor aqueous solubility limits bioavailability and practical applications. In this study, the acid-modified citrus pectin fraction MCP-2 was used as a natural solubilizing matrix to improve the aqueous solubility of quercetin. Quercetin solubility reached a maximum at 1.0 mg/mL MCP-2, approximately 3.9-fold that in water. Methylation analysis and nuclear magnetic resonance (NMR) spectroscopy indicated that the MCP-2 backbone was mainly composed of galactopyranuronic acid (GalpA), methyl-esterified GalpA, and galactopyranosyl (Galp) residues, with minor rhamnopyranosyl (Rhap) residues and short GalpA-containing side chains attached to Rhap units. X-ray diffraction, differential scanning calorimetry, Fourier-transform infrared spectroscopy, and molecular dynamics simulations provided consistent evidence for the formation of a stable MCP-2/quercetin complex through noncovalent interactions. Compared with quercetin and the MCP-2/quercetin physical mixture, the MCP-2/quercetin complex exhibited stronger antioxidant activity in chemical radical-scavenging assays and showed improved protection against H 2 O 2 -induced oxidative injury in HepG2 cells. Overall, this study elucidates the mechanism of MCP-2-mediated quercetin solubilization and provides a natural polysaccharide-based strategy for improving the aqueous solubility and functional performance of poorly water-soluble flavonoids. Download: Download high-res image (346KB) Download: Download full-size image
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2026
·
IF 5.4
Zhonghao Jia,Yaru Sun,Pengfei Xiao,Zhe Cui,Yong Wang,Chengyan Zhou,Baohua Chen,Jianzhong Xu,Jianglei Qin
In this study, the pectin hydrazide bearing boronic acid groups (PHBA) was synthesized through coupling and hydrazinolysis reaction successively. In addition, the dopamine (DA) was grafted onto oxidized dextran (ODex) through dynamic Schiff-base connection to endow the resultant DA grafted ODex (ODDA) with mussel inspired tissue adhesive property and antioxidant property. The double network self-healing PHBA/ODDA hydrogel (Gel) with a variety of advantages was fabricated from PHBA and ODDA with dynamic hydrazone bonds and boronic ester bonds. The graphene oxide (GO) as photothermal agent was composited into the hydrogel to enhance the antibacterial performance stimulated by near infrared (NIR) radiation. Meanwhile, the epigallocatechin gallate (EGCG) as an antioxidant and anti-inflammatory drug was loaded to take the advantage of sustained release behavior of the hydrogel to promote the wound healing. The results showed the composite hydrogel greatly enhance the antioxidant property and antibacterial property with the biocompatibility well preserved. Under NIR irradiation and the activity of the loaded EGCG, the GO@EGCG/Gel inhibited the infection and inflammation of the wound to promote the healing progress on mice model. In conclusion, the Gel can act as a good platform as GO and EGCG carrier to promote the wound healing as potential dressing material. Download: Download high-res image (202KB) Download: Download full-size image
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