MOLECULES
·
2026
·Vol. 31
·期 18
·3194
·
IF 5.1
Henan Sun,Hao Yu,Huiqiang Yu,Gaowa Saren,Ke Feng,Wenzhong Hu
A water-soluble, low-molecular-weight polysaccharide fraction, designated OJP-2, was extracted from the roots ofOphiopogon japonicus; its structural characteristics and immunomodulatory activity in macrophages were subsequently investigated. OJP-2 exhibited a narrow apparent molecular weight distribution with an average molecular weight (Mw) of 3568 Da and was primarily composed of fructose (0.784) and glucose (0.208). Structural analysis—integrating UV spectroscopy, Fourier-transform infrared (FT-IR) spectroscopy, and 1D/2D nuclear magnetic resonance (NMR) spectroscopy—revealed that OJP-2 is an inulin-type fructan. Its structure is characterized predominantly by β-(2→1)-linked Fruf chains and terminal α-D-Glcp residues, with potential signals indicating C-6-substituted Fruf units. In vitro immunological studies demonstrated that OJP-2 promoted nitric oxide (NO) production and enhanced the secretion of IL-6, IL-1β, and TNF-α in RAW264.7 macrophages. Under LPS/IFN-γ stimulation, OJP-2 induced non-monotonic changes in the CD86/CD206 macrophage phenotype, with the most pronounced effects observed at a concentration of 50 μg/mL. Furthermore, Western blot analysis showed that, compared to the Model group, treatment with OJP-2 resulted in a downward trend in the relative levels of p-p65/p65 and p-IκBα/IκBα across the tested concentration range. Collectively, these findings indicate that OJP-2 modulates macrophage activation phenotypes and influences NF-κB-related signaling pathways. Thus, OJP-2 is a candidate fructan for further investigation of immunomodulatory activity.
✓ 已验证
DOI
高影响因子
MOLECULAR CELL
·
2026
·
IF 16
Chen Nie,Wanchang Liu,Lei Zhang,Sai Ge,Mingyang Ma,Jundi Wang,Zhanzhan Xu,Yingyu Qin,Xiao Albert Zhou,Yang Liu,Weibin Wang,Jiadong Wang
Genomic integrity in transcriptionally active regions is pivotal for suppressing oncogenic mutations, yet the mechanisms that govern precise homologous recombination (HR) repair within these regions remain elusive. Here, we report that the IRAK1-spliceosome axis operates with small nuclear RNA (snRNA) as a central hub, potently promoting accurate repair at DNA double-strand break (DSB) sites within active chromatin in human cancer cells. Mechanistically, IRAK1 phosphorylates spliceosomal serine/arginine (SR)-rich proteins to recruit snRNA to DSBs, inducing robust condensation of the MRE11-RAD50-NBS1 (MRN) complex near transcriptionally active regions to create an ATM activation platform. Collectively, our findings define a prevalent mechanism governing region-specific precise repair in transcriptionally active domains, where snRNA acts as a “transcription repair bridge” to link transcriptional processes to HR repair and ultimately preserves genomic stability. Inhibiting IRAK1 axis impairs HR repair in transcriptionally active regions, causing a marked increase in mutation rates specific to these regions and cancer-cell chemosensitivity.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Drug Design Development and Therapy
·
2026
·
IF 6.1
Weiyi Zhang,Peiqi Wang,Yaoyao Cai,Chengyao Feng,Jiaojiao Dong,Zhousheng Jin,Fangfang Xia
Background Ropivacaine, a local anesthetic, is commonly used for surgical local anesthesia and labor analgesia.Purpose This study aimed to investigate the effect of apatinib on ropivacaine metabolism in vitro and in vivo, and to elucidate its inhibitory mechanism.Methods Following preliminary screening of 18 candidate drugs, apatinib was selected for further evaluation. An enzymatic reaction system was established using rat liver microsomes (RLM), human liver microsomes (HLM), and recombinant human CYP1A2 (rCYP1A2). The ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed for the simultaneous quantification of ropivacaine and its metabolite, 3-hydroxy ropivacaine.Results The UPLC-MS/MS method showed good linearity over 2–250 ng/mL for ropivacaine and 1–25 ng/mL for 3-hydroxy ropivacaine, respectively. Both showed intra- and inter-day precision (RSD%) below 15% and accuracy (RE%) within ± 15%. Apatinib inhibited ropivacaine metabolism in RLM, HLM, and rCYP1A2, with half-maximal inhibitory concentration (IC50) values of 0.29, 9.12, and 2.49 μM, respectively. In the RLM metabolic stability assay, apatinib slowed ropivacaine metabolism and reduced its intrinsic clearance (CLint) from 0.14 to 0.04 mL/min/mg. Enzyme kinetic analysis indicated non-competitive inhibition in all systems. In rats, co-administration with apatinib increased the area under the plasma concentration-time curve (AUC) and maximum plasma concentration (Cmax) of ropivacaine by 0.83- and 0.71-fold, respectively, decreased the clearance (CLz/F) by 47.17%.Conclusion Both in vitro and in vivo results indicated that apatinib inhibited the metabolism of ropivacaine. These findings provided essential reference data for the rational application of ropivacaine in clinical precision dosing regimens.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Polymers
·
2026
·Vol. 18
·期 17
·2072
·
IF 5.8
Jia-Hao Wang,Ze-Yong Zhao,Yu-Zhong Wang
Polymeric antistatic additives offer improved resistance to migration compared with low-molecular-weight agents, but high loadings are generally required to establish effective charge-dissipation pathways in nonpolar polypropylene (PP). Herein, a series of quaternary ammonium salt-functionalized polyamide 6/polyethylene glycol elastomers (QASPA6PEG) was synthesized by melt copolymerization and used as multifunctional antistatic additives for PP. Increasing the nominal QAS content decreased the surface resistivity of the elastomers from 3.24××109Ω to 9.71 × 108Ω. The elastomers were subsequently melt-blended with PP at loadings of 10–20 wt% using maleic-anhydride-grafted polypropylene as a compatibilizer. The surface resistivity of the blends decreased with increasing QAS content and elastomer loading, consistent with the formation of increasingly interconnected ion-conducting domains. The blend containing 20 wt% 0.4QASPA6PEG exhibited surface resistivities of 3.64××1011Ω and 4.69××1010Ω on days 0 and 60, respectively. Its saturated water absorption reached 4.38%, compared with 0.27% for neat PP, supporting a moisture-assisted ionic conduction mechanism. The measured bromine content remained nearly unchanged after 60 days of storage, indicating limited loss of the QAS-containing component. In addition to improving charge dissipation, QASPA6PEG enhanced the ductility and impact resistance of PP. At a loading of 20 wt%, 0.4QASPA6PEG increased the elongation at break from 358 ± 23% to 690 ± 81% and the notched impact strength from 3.16 ± 0.37 to 4.93 ± 0.45 kJm−2. These results demonstrate that covalently introducing ionic structures into PA6/PEG elastomers is an effective strategy for coupling antistatic modification with toughening in PP.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomedicines
·
2026
·Vol. 14
·期 9
·2005
·
IF 4.5
Qiuyue Song,Fangrui Liu,Jinyan Zhao,Wenli Xie,Xianjun Fu,Kuo Xu
Background:The zebrafish model is extensively utilized for phenotypic screening, particularly in heart failure research. However, traditional photography-based data collection requires tricaine methanesulfonate (MS-222) anesthesia, which can suppress cardiac function and introduce artifacts. This study presents a microfluidic platform designed to facilitate anesthesia-free, noninvasive in vivo photography of zebrafish heart failure models.Methods:Isoproterenol (ISO) induced heart failure in 1 day post-fertilization (dpf) zebrafish embryos. Larvae were assigned to three groups: Control (E3 embryo medium only), Model (1 mM ISO for 48 h from 1 dpf), and Positive (1 mM ISO + 10 μM propranolol for 48 h from 1 dpf). Subsequently, a microfluidic chip featuring tapered immobilization channels was adopted. The chip facilitated the photography and collection of cardiac function parameters from lateral and supine positions, including cardiac output (CO), blood flow velocity (BFV), ejection fraction (EF), stroke volume (SV), and fractional shortening (FS). All imaging was performed in completely independent parallel experiments, with no individual larva shared between the two imaging methods. Data obtained under hydrodynamic confinement were compared with those acquired under MS-222 anesthesia.Results:Cardiac parameters measured using the microfluidic platform were significantly higher than those under MS-222 anesthesia: CO (0.339 ± 0.032 vs. 0.279 ± 0.023 nL/s,p< 0.001), BFV (681 ± 53 vs. 604 ± 38 μm/s,p< 0.001), EF (10.3 ± 0.7% vs. 6.6 ± 0.7%,p< 0.001), SV (184,519 ± 12,822 vs. 97,979 ± 5306 μm3,p< 0.001), and FS (7.3 ± 1.8% vs. 3.7 ± 0.9%,p< 0.001). Strong correlations (R2> 0.85,p< 0.0001) revealed that anesthesia underestimated parameters by 11.3–49.3%.Conclusions:Compared with the traditional anesthesia-based method, the microfluidic platform enables more precise cardiac parameter collection in a zebrafish heart failure model than when anesthesia is used, offering a reliable tool for phenotypic screening of zebrafish embryos.
✓ 已验证
DOI
高影响因子
INTERNATIONAL DENTAL JOURNAL
·
2025
·
IF 3.7
Xin-Lu Li,Hui Liu,Wei Fan,Bing Fan
Introduction and aims To develop a multifunctional nanofiber membrane carrying angiogenic, dentinogenic, and neurogenic growth factors for dental pulp regeneration. Methods Electrospun gelatin/polycaprolactone (GEL/PCL) nanofiber membranes were modified with heparin (H-GEL/PCL) and loaded with VEGF, BMP-2, and NGF. The physicochemical properties and growth factor release behaviour of the membranes were systematically characterized. Their bioactivity was evaluated by assessing cell viability, proliferation, migration, and differentiation of human dental pulp stem cells in vitro, and a subcutaneous semiorthotopic transplantation model was established to verify the pulp-like tissue regeneration potential in vivo. Results The H-GEL/PCL membranes exhibited uniform nanofiber morphology, favourable mechanical and hydrophilic properties, sustained degradation, and controlled growth factor release. H-GEL/PCL membranes loaded with VEGF, BMP-2, and NGF enhanced angiogenic, odontogenic, and neurogenic differentiation of dental pulp stem cells in vitro and facilitated vascular and neural ingrowth of dental pulp-like tissue in vivo. Conclusions The H-GEL/PCL membrane carrying angiogenic, dentinogenic, and neurogenic growth factors effectively promotes angiogenesis and neurogenesis in the regenerated dental pulp-like tissue. Clinical relevance This multifunctional membrane offers a promising strategy for regenerating vascularized and innervated dental pulp-like tissues, with potential application for regenerative endodontic therapy.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Today Bio
·
2025
·
IF 10.2
Chenhui Zhou,Fangkun Yang,Chunyan Li,Hengyi Mao,Kai Wang,Jinhui Shang,Xiang Gao,Wenming He
Atherosclerosis (AS) remains a major global health concern, contributing significantly to cardiovascular morbidity and mortality. Recent research into enzymes like aspartyl aminopeptidase (DNPEP) has shed light on its potential role in regulating inflammation and protein metabolism, offering new avenues for targeted diagnostics and therapies. In light of these challenges, this study aims to develop a novel optical imaging probe (HD-DNPEP) specifically designed to detect DNPEP expression in atherosclerotic lesions. By addressing the current limitations in sensitivity, specificity, and in vivo detection, we seek to create a highly selective and sensitive imaging tool that can provide real-time, high-resolution insights into the role of DNPEP in atherosclerosis. This innovative approach has the potential in the diagnosis of atherosclerosis, enabling more precise intervention strategies and improving patient outcomes. In AS patients, the significantly higher DNPEP levels in the cerebral vasculature and CSF of AS patients suggest that DNPEP may serve as an important biomarker for detecting cerebrovascular arteriosclerosis.
✓ 已验证
DOI
PubMed
高影响因子
BIOMATERIALS
·
2025
·
IF 12.9
Lamei Liu,Min Zhang,Yan Zhou,Lin He,Mingjian Chen,Yuchen Wu,Guanyu Yin,Hui He,Lanlin Qi,Bin Zhang,Xiaoxiao He,Kemin Wang
Osteoarthritis (OA) is a complex and multifactorial joint disorder. Currently, there are few therapies to slow the progression of OA. Combining symptomatic slow-acting drugs and reactive oxygen species (ROS) scavenging reagents in the inflamed joint shows a promising therapeutic approach for OA. Nonetheless, the short residence time of free drugs in the joint cavity hinders their further application. Herein, an injectable composite of hydrogel and chondroitin sulfate@resveratrol liposome package (Gel/Lip@Res + Chs) was developed to improve drug residency ability and enable the simultaneous delivery of hydrophilic Chs and hydrophobic Res in the joint cavity. This injectable hydrogel, based on phenylboronic acid modified hyaluronic acid (HAPBA) and polyvinyl alcohol (PVA), provided ROS-responsive decomposition property and sustained drug release behavior, thereby effectively scavenging ROS, promoting macrophage repolarization, and decreasing the expression of inflammatory mediators and matrix-degrading enzymes in vitro . As a proof of concept, the in vivo therapeutic efficacy of Gel/Lip@Res + Chs was further examined in both monoiodoacetic acid (MIA)-induced and surgical anterior cruciate ligament transection (ACLT)-induced OA mouse models. Benefiting from the extended residence time of loaded drugs in the joint cavity, the Gel/Lip@Res + Chs treatment could effectively suppress cartilage degeneration and increase the synthesis of the cartilage matrix, thus slowing the advancement of OA. The present study provides a reference for improving drug retention and holds great potential for treating OA-related diseases.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Science
·
2020
·Vol. 7
·期 21
·1902740
·
IF 15.84
Mohammad Ali Darabi,Ali Khosrozadeh,Ying Wang,Nureddin Ashammakhi,Halima Alem,Ahmet Erdem,Qiang Chang,Kaige Xu,Yuqing Liu,Gaoxing Luo,Ali Khademhosseini,Malcolm Xing
Strong, stretchable, and durable biomaterials with shape memory properties can be useful in different biomedical devices, tissue engineering, and soft robotics. However, it is challenging to combine these features. Semi-crystalline polyvinyl alcohol (PVA) has been used to make hydrogels by conventional methods such as freeze–thaw and chemical crosslinking, but it is formidable to produce strong materials with adjustable properties. Herein, a method to induce crystallinity and produce physically crosslinked PVA hydrogels via applying high-concentration sodium hydroxide into dense PVA polymer is introduced. Such a strategy enables the production of physically crosslinked PVA biomaterial with high mechanical properties, low water content, resistance to injury, and shape memory properties. It is also found that the developed PVA hydrogel can recover 90% of plastic deformation due to extension upon supplying water, providing a strong contraction force sufficiently to lift objects 1100 times more than their weight. Cytocompatibility, antifouling property, hemocompatibility, and biocompatibility are also demonstrated in vitro and in vivo. The fabrication methods of PVA-based catheters, injectable electronics, and microfluidic devices are demonstrated. This gelation approach enables both layer-by-layer and 3D printing fabrications.
研究领域:
生物材料学
·
组织工程
·
软体机器人
·
再生医学
·
生物医学工程
·
高分子科学
·
转化医学
✓ 已验证
DOI
高影响因子
Sustainable Energy & Fuels
·
2023
·Vol. 8
·期 2
·358-368
·
IF 5.6
Hai Lu,Peichun Wang,Yitian Ma,Meng Liu,Linqing Chang,Rui Feng,Shuliang Luo,Zhiyun Zhang,Yi Wang,Yan Yuan
In this study, quasi-solid polymer electrolytes incorporating an ionic liquid (ILQSEs) fabricated in different organic solvents via a thermally-initiated polymerization strategy were investigated for flexible supercapacitors (SCs). It was found that low-viscous acetonitrile (AN) with a strongly-polar nitrile group was remained in the polymer matrix, which favored a higher ionic conductivity, wider electrochemical window, and better thermal stability of the ILQSE compared to its propylene carbonate (PC) counterpart. Moreover, the in situ polymerized quasi-solid electrolyte achieved close contact with the porous carbon electrode, accompanied by the favorable permeation of the IL into the electrode for enhancing the ion-transport kinetics and interface stability. Consequently, the as-assembled SC with the AN-based ILQSE delivered a high specific energy and specific power, excellent cycle stability, and reliable safety.
研究领域:
材料科学
·
电化学
·
高分子化学
·
能源存储
·
纳米科技
✓ 已验证
DOI
高影响因子
COMBUSTION AND FLAME
·
2021
·
IF 4.185
Zhi-Hao Jin,Jin-Tao Chen,Wang Li,Shu-Bao Song,Jiu-Zhong Yang,Zhen-Yu Tian
Owing to significant advantages over traditional biofuels, 2-methylfuran (MF) has arisen extensive interest as next generation biofuels . Atmospheric pressure pyrolysis of a 1:1 blend of MF with a three-component (3C) surrogate jet fuel was conducted in a flow tube using synchrotronphotoionization and molecular beam mass spectrometry techniques within a temperature range of 898–1223 K. Therein, the 3C surrogate fuel consists of 66.2% n-dodecane (NC 12 H 26 ), 18.0% 1,3,5-trimethylcyclohexane (T135MCH) and 15.8% n-propylbenzene (NPB, A1C 3 H 7 ). The detailed kinetic model including 579 species and 3474 reactions was developed via validating against both measured and literature data with reasonable agreement. MF exhibits no obvious influence on the 3C components pyrolysis . However, 3C mixtures play an important roles in MF decomposition: lower initial temperature and different decomposition pathways. In the meantime, n-propylbenzene (NPB) pyrolysis curve in 3C and 3C+MF significantly transfers to low temperature zone after 1000 K in contrast to pure NPB, while smaller change was found in the decomposition curve of n-dodecane. Compared with 3C pyrolysis, 3C+MF blends have less formation of aromatic species and light hydrocarbons except for vinylacetylene (C 4 H 4 ) and acetylene (C 2 H 2 ). Allyl radical (C 3 H 3 ) has significant roles in benzene ring formation. The combination of allyl radical with propargyl radical producing benzene and H radical (aC 3 H 5 + C 3 H 3 = A1 + 2H) has the highest contribution to the benzene formation in both 3C and 3C+MF. Moreover, most of naphthalene (A2) formation is also related to C 3 H 3 radical via reaction: A1CH 2 + C 3 H 3 = A2 + H, rather than via HACA (hydrogen abstraction carbon addition) reaction. Interaction of fuel blends was mainly presented through the common and important sensitive reactions which are much to do with CH 3 and H radicals. The overview of product distribution was investigated by providing comparisons of carbon conversion rates during the pyrolysis of 3C and 3C+MF. These results will be helpful to better understand the pyrolysis properties of MF/jet fuel surrogate blends and could contribute to developing new generation biofuels for aviation.
研究领域:
燃料化学
·
燃烧动力学
·
生物燃料
·
能源化学
·
反应机理
·
质谱分析
✓ 已验证
DOI
高影响因子
SURFACE & COATINGS TECHNOLOGY
·
2016
·
IF 2.139
Xiangyang Chen,Guangxin Xiang,Fengtao Chai,Zhijun Zhang,Siqi Shi,Fangfang Xu,Jingtai Zhao
Europium-doped Lutetium oxide (Lu 2 O 3 : Eu 3 + ) polycrystalline films have been successfully fabricated on cleaned silicon (100) substrates by routine and alternate spin-coating techniques. X-ray diffraction (XRD), photoluminescence (PL) spectra, field emission electron scanning microscope (FE-SEM) are employed to characterize the as-fabricated films. The SEM observation reveals that the thickness and porosity of the film fabricated by the alternate spin-coating are superior to that by routine spin-coating, i.e. the thickness and porosity increase 95% and reduce 26%, respectively, resulting in 72% enhancement of the emission intensity. According to theoretical calculation and crystallographic analysis, the dominated 5 D 0 - 7 F 2 emission of Eu 3 + can be assigned to the main non-inversion site occupancy.
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）