BIORESOURCE TECHNOLOGY
·
2015
·
IF 4.494
Yu-Cai He,Dan-Ping Zhang,Zhi-Cheng Tao,Yun Lu,Yun Ding,Feng Liu,Zheng-Zhong Zhu,Huan Rui,Gao-Wei Zheng,Xian Zhang
To reduce dependence on the expensive cofactor and effectively biotransform ethyl 4-chloro-3-oxobutanoate, l -glutamine and glycine were found to enhance the content of intracellular NADH and the reductase activity. Adding the mixture of 200 mM of l -glutamine and 500 mM of glycine to the reaction media, a 1.67-fold of reductase activity was increased over the control without the addition of the two compounds. Moreover, β-cyclodextrin (0.4 mol β-cyclodextrin/mol ethyl 4-chloro-3-oxobutanoate) was also added into this reaction media, and the biocatalytic activity of the whole-cell biocatalyst of Escherichia coli CCZU-K14 was increased by 1.34-fold than that without β-cyclodextrin. In this β-cyclodextrin–water media containing l -glutamine (200 mM) plus glycine (500 mM), ethyl ( S )-4-chloro-3-hydroxybutanoate (>99% ee ) could be obtained from 3000 mM ethyl 4-chloro-3-oxobutanoate in the yield of 98.0% after 8 h. All the positive features demonstrate the potential applicability of the bioprocess for the large-scale production of ethyl ( S )-4-chloro-3-hydroxybutanoate.
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Food Bioscience
·
2025
·
IF 5.9
Shuai Wang,Jiayang Li,Cong Xie,Wanhui Chen,Huajie Feng,Wenying He
Our previous study found that 1-(4-bromophenyl)-5-phenyl-1H-1,2,3-triazole (BPT) exhibited very low toxicity and higher biology activity. In the present study, BPT was used to investigate its mechanism against Colletotrichum gloeosporioides in mango by chemical proteomics and chemicobiological methods. The experimental results of some phenotypic traits and cytotoxicity indicated that there are some changes in permeability of the cell membranes and BPT has fungistatic activity with lower cytotoxicity. Next, a biotin probe named BPTN with a higher activity (EC 50 value of 11.08 mg/L) than that of BPT(EC 50 value of 14.13 mg/L) was designed and synthesized by the chemical proteomics approach. The major differentially expressed proteins between BPT or BPTN and Colletotrichum gloeosporioides were identified and analyzed by Matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI-TOF-MS) and bioinformatics. The results indicated that there are 25 differentially expressed proteins in Colletotrichum gloeosporioides under BPT intervention, and 29 target proteins in Colletotrichum gloeosporioides captured by BPTN. For BPTN-anthracnose system, the protein No. 8 belonged to cytochrome P450 proteins (CYP) family can be taken as a target protein of BPTN, which confirmed by bioinformatics analysis. Further, a classic fungal target protein of triazoles, sterol 14α-demethylase (CYP51) was used to verify the interaction in the presence of BPT by various spectroscopic and molecular docking methods. The results showed that BPT exhibited strong interactions with CYP51 and it was predominantly driven by hydrophobic forces and hydrogen bonds. The present study has significant theoretical and practical implications for the development of novel 1,2,3-triazole antifungal agents.
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INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2020
·
IF 4.939
Wei Cheng,Xue Zhao,Wenxiu Luo,Yi Wang,Guangyin Fan
Oxygen and nitrogen-rich functional groups which are generally present in carbon materials may significantly affect their catalytic properties . The development of O, N-rich functional carbon materials with a simplified and low-budget strategy is of great importance for practical application. Herein, we describe a facile and straightforward method to prepare high contents of nitrogen and oxygen-rich carbon derived from ethylenediamine tetra-acetic acid tetra-sodium salt (Na 4 EDTA) by one-step carbonization in muffle furnace without inert gas protection. Compared with the carbon (EC-N700) synthesized in an argon atmosphere , the carbon (EC-A700) synthesized in an air-rich condition was more conducive to the formation of edge defects and topological defects and possessed higher contents of N and O elements. Consequently, the O, N-rich carbon EC-A700 was used as an efficient support of palladium nanoparticles toward catalytic formic acid dehydrogenation and nitroarene reduction. The catalyst Pd/EC-A700 achieved outstanding activity for formic acid dehydrogenation with a turnover frequency (TOF) up to 7039 h −1 at 60 °C. At the same time, the obtained catalyst Pd/EC-A700 also demonstrated exceptional catalytic performance in the 4-nitrophenol (4-NP) reduction with corresponding rate constant k of 0.72 min −1 under mild reaction conditions, which proved that the prepared catalyst Pd/EC-A700 has potentially applied in catalytic hydrogenation. This low-cost and simple approach breaks the tradition in mind that pyrolysis should always operate under inert conditions and provides a straightforward route to construct O, N-rich carbon as appealing materials for a wide variety of applications.
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RSC Advances
·
2016
·Vol. 6
·期 114
·113352-113365
·
IF 3.289
Cong Liu,Dongxiang Zhang,Liting Zhao,Xin Lu,Peng Zhang,Shengnan He,Guowen Hu,Xiuqin Tang
A thiacalix[4]arenetetrasulfonate-functionalized reduced graphene oxide (TCAS–rGO) adsorbent was synthesized and used as an adsorbent for the removal of lead(II) and cadmium(II) from aqueous solutions. TCAS–rGO was characterized by Fourier transform infrared (FTIR) spectroscopy, Raman spectroscopy, X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), energy dispersive X-ray (EDX) analysis and thermal gravimetric analysis (TGA). The adsorption of Pb(II) and Cd(II) onto TCAS–rGO as a function of pH, contact time, metal ion concentration, and temperature was investigated. Results showed that the adsorption of Pb(II) and Cd(II) onto TCAS–rGO was greatly influenced by the pH. TCAS–rGO shows excellent selective adsorption for Pb(II) and Cd(II) compared with other metals Na(I), K(I), Mg(II) and Sr(II) at neutral pH. The adsorption reached equilibrium within 4 h and the kinetics could be described well by a pseudo-second-order model. The adsorption isotherms for Pb(II) and Cd(II) were fitted by the Langmuir model, and the maximum adsorption capacities of the adsorbent were 230 mg g−1 for Pb(II) and 128 mg g−1 for Cd(II). Thermodynamic studies indicate that the adsorption was a spontaneous and endothermic process in nature. The chloride and nitrate anions may barely influence the adsorption. The TCAS–rGO can be reused as an adsorbent following 4 cycles of adsorption/desorption. Possible adsorption mechanisms are also analysed. This paper provides a potential application of the TCAS–rGO composite in heavy metal treatment.
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ENVIRONMENTAL POLLUTION
·
2020
·
IF 6.793
Cui Ma,Shengyong Jia,Pengfei Yuan,Zhengguang He
Composite magnetic oxide at cow dung ash, nano-Fe 3 O 4 @cow dung ash (nano-Fe 3 O 4 @CDA), was used as catalytic material for the degradation of 2, 2′-methylenebis (4-methyl-6-tert-butylphenol) (AO 2246) in real biologically pretreated landfill leachate . The Fe 3 O 4 @CDA composite exhibited catalytic ozonation activity and allowed material separation and magnetic recovery. The effects of several operating parameters including O 3 concentration, catalyst dosage, temperature and scavengers were evaluated in parallel. Over 70% of AO 2246 were removed by the nano-Fe 3 O 4 @CDA/O 3 system under optimum conditions within 120min reaction time. The EPR, GC–MS and free-radical quenching experiments expatiated the mechanism of this degradation process. It was confirmed that the AO 2246 was degraded efficiently in this catalytic micro-ozonation process, Additionally, GC–MS analysis state clearly that the 3,5-bis(1,1-dimethylethyl)phenol, 4-(1,5-dihydroxy-2,6,6-trimethylcyclohex-2-enyl)but-3-en-2-one, ethanone, 1-(1,4-dimethyl-3-cyclohexen-1-yl)-, 5-tert-butyl-6-3, 5-diene-2-one, 2-hydroxyhexanoic acid, 2-propenoic acid 1,1-dimethylethyl ester, butanoic acid, 2-methyl-, methyl ester and propanoic acid, 2, 2-dimethyl- were the dominant oxidation products (OPs) during the degradation of the AO 2246. The EPR results showed that the catalytic ozonation over Fe 3 O 4 @CDA led to produce more hydroxyl radicals , which were in favor of AO 2246 degradation. The toxicity evolution was also performed through a QSAR analysis calculated by the ECOSAR program which further demonstrated the different responses toward the AO 2246 and its OPs.
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JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Jiaohui Xia,Yan Li,Xinbai Jiang,Dan Chen,Jinyou Shen
Electricity-stimulated anaerobic system (ESAS) has shown great potential for halogenated organic pollutants removal. Exogenous redox mediators can improve electron transfer efficiency to enhance pollutants removal in ESAS. In this study, humic acid (HA), a low-cost electron mediator, was added into ESAS to enhance the simultaneous reductive debromination and mineralization of 4-bromophenol (4-BP). Results showed that the highest 4-BP removal efficiency at 48 h was 95.43 % with HA dosage of 30 mg/L at − 700 mV, which was 34.67 % higher than that without HA. The addition of HA decreased the requirement for electron donors and enriched Petrimonas and Rhodococcus for humus respiratory. HA addition regulated microbial interactions, and enhanced species cooperation between Petrimonas and dehalogenation species ( Thauera and Desulfovibrio ), phenol degradation-related species ( Rhodococcus ) as well as fermentative species ( Desulfobulbus ). Functional genes related to 4-BP degradation ( dhaA/hemE/xylC/chnB/dmpN ) and electron transfer ( etfB/nuoA/qor/ccoN/coxA ) were increased in abundance by HA addition. The enhanced microbial functions, as well as species cooperation and facilitation, all contributed to the improved 4-BP biodegradation in HA-added ESAS. This study provided a deep insight into microbial mechanism driven by HA and offered a promising strategy for improving halogenated organic pollutants removal from wastewater.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Mengru Zhao,Minghui Sun,Qi Kang,Xuemei Ma,Dazhong Shen
Herein, we report a differential strategy to enhance the anti-interference ability of the electrochemical molecularly imprinted polymers (MIP) based sensors for the determination of 4-acetamidophenol (AP) and sulfamerazine (SMR). Polypyrrole MIP membranes for AP or SMR were grown independently on the surface of Ni 2 P/GCE, with the imprinting factors of 6.13 for MIP AP and 5.82 for MIP SMR . At the potentials of 0.42 V and 0.89 V (vs. Ag/AgCl), MIP AP /Ni 2 P/GCE and MIP SMR /Ni 2 P/GCE exhibit sensitive current responses to AP and SMR, respectively. However, the non-specific adsorption of various interferents on the surface of MIP suppresses the selectivity of the MIP sensors. For example, 20 μM ascorbic acid (AA) and sulfamethoxazole (SMZ) were determined falsely as 5.2 μM AP and 4.7 μM SMR, respectively. In the proposed differential strategy using the current differences between MIP AP /Ni 2 P/GCE and MIP SMR /Ni 2 P/GCE as the signal indicators, 20 μM of AA and SMZ are equivalent only to 0.25 μM AP and 0.33 μM SMR, respectively, enhancing significantly the anti-interference ability of MIP sensors. Moreover, the limit of detection for AP and SMR are 0.011 and 0.019 μM, respectively. AP and SMR in actual urine samples were determined with the recoveries from 94% to 105%.
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ZEITSCHRIFT FUR NATURFORSCHUNG SECTION B-A JOURNAL OF CHEMICAL SCIENCES
·
2020
·
IF 0.839
Gao-Feng Wang,Shu-Wen Sun,Hong Sun,Bing-Wei Chang,Jing Song,Xia Yang
Two coordination polymers (CPs), [Mn(bipmo)(pbtda)(H 2 O)] n ( 1 ), {[Cd(bipmo)(btda)]·2(H 2 O)]} n ( 2 ), (bipmo = bis(4-(1 H -imidazol-1-yl)phenyl)methanone, H 2 pbtda = 2-phenylbutanedioic acid, H 2 btda = butanedioic acid), were synthesized by solvothermal methods. The products have been characterized by elemental analyses, IR spectroscopy, and single-crystal X-ray diffraction. The topological structure of 1 is based on 2-nodal nets with the Schläfli symbol {6 3 }{6 9 ·8}, while that of 2 features a system with a sixfold penetration with the Schläfli symbol {6 6 }.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2015
·
IF 3.368
Pancras Ndokoye,Qidong Zhao,Xinyong Li,Tingting Li,Moses O. Tade,Shaobin Wang
In this study, we demonstrate for the first time that highly branched gold nanostars (AuNSs) and silica-coated AuNSs (AuNSs@mSiO 2 ) could potentially serve as efficient hydrogenation catalysts. The catalytic activity could be promoted by raising the number of tipped-branches of AuNSs, which reveals that the tips play an important role as active sites. The fabricated sharply-pointed AuNSs benefit the electron transfer from BH 4 anions to 4-nitrophenol. Coating AuNSs with mesoporous silica (AuNSs@mSiO 2 ) further enhanced the reduction rate and recyclability, and also contributed to reducing the induction period. The AuNSs@mSiO 2 (50–100 nm in diameter) are large enough to be catalytically inactive, but they consist of sharply-pointed tips with the radius of 2.6–3.6 nm, which are rich in coordinately unsaturated sites similar to those of nanoparticles and clusters. Such features in structure and activity would also extend their application range in heterogeneous catalysis .
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JOURNAL OF POLYMER SCIENCE
·
2023
·Vol. 61
·期 14
·1486-1494
·
IF 3.4
Chenqi Zhao,Penghua Yan,Ruofan Liu,Wei Li,Yushun Jin,Qisong shi,Yibo Wu
The cationic polymerization of isobutene vinyl ether(IBVE) contains waters system was achieved by using triphenylmethylium tetrakis-(pentafluorophenyl)borate [Ph 3 C][B(C 6 F 5 ) 4 ], which overcame the harsh experimental requirements of traditional cationic polymerization. The influences of water content, solvent polarity, and reaction temperature on polymerization were studied. The end structure of the polymer was analyzed by 1 H-NMR. Combined with the effect of the proton trapping agent and triethylamine salt which can trap [H + ], it was found that there was a reversible equilibrium between [Ph 3 C + ] and [H + ] active species during the polymerization process. The cationic polymerizations of isobutyl vinyl ether were initiated by these two active species simultaneously. The controllable cationic polymerization of isobutyl vinyl ether was achieved by the addition of ethyl methyl carbonate and ethyl benzoate. Gaussian 09 W simulation confirms the polymerization mechanism. Graphical
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CERAMICS INTERNATIONAL
·
2024
·
IF 5.1
Chunyang Zhang,Yifan Wang,Zexiong Wang,Shilong Zhao,Ruoshan Lei,Shiqing Xu
The development of ultrasensitive thermometric phosphors has become an active area of research due to their appealing applications in the contactless temperature detection field. Herein, the scheelite-related compounds—CaGd 2 (WO 4 ) 4 : Nd 3+ , Yb 3+ and CaGd 2 (MoO 4 ) 4 : Nd 3+ , Yb 3+ , which exhibit high sensing capacity—are successfully prepared and systematically characterized. The X-ray diffraction analysis reveals that CaGd 2 (WO 4 ) 4 : Nd 3+ , Yb 3+ exhibits the monoclinic structure, whereas CaGd 2 (MoO 4 ) 4 : Nd 3+ , Yb 3+ is in the tetragonal phase. The maximum phonon cut-off energy of CaGd 2 (WO 4 ) 4 : Nd 3+ , Yb 3+ is 929 cm −1 , slightly larger than that of CaGd 2 (MoO 4 ) 4 : Nd 3+ , Yb 3+ (904 cm −1 ). Irradiated by 980 nm laser, PL spectra consist of the Nd 3+ : 4 F j → 4 I 9/2 (j = 7/2, 5/2, 3/2) and Yb 3+ : 2 F 5/2 → 2 F 7/2 emissions located in the near-infrared (NIR) range. Negative and positive thermal quenching behaviors are observed for the emissions of Nd 3+ (754, 802, and 868 nm) and Yb 3+ (1008 nm), respectively, attributed to the cooperative effects of the phonon-assisted energy transfer process of Yb 3+ → Nd 3+ and excited state absorption process of Nd 3+ . Moreover, the host material can affect the luminescence properties, thermal enhancement factor of the NIR anti-Stokes emission, and thermal sensing performance of the Nd 3+ -Yb 3+ pair. Consequently, CaGd 2 (MoO 4 ) 4 : Nd 3+ , Yb 3+ exhibits higher sensing sensitivity (S r-max = ∼6.00%K −1 ) than CaGd 2 (WO 4 ) 4 : Nd 3+ , Yb 3+ (S r-max = ∼5.56%K −1 ). This study provides useful information for the construction of high-performance ratiometric thermometers based on the inverse thermal dependence of the NIR emissions of Nd 3+ and Yb 3+ ions.
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JOURNAL OF LUMINESCENCE
·
2024
·
IF 3.3
Chenglong Xia,Jiahui Yang,Bingyang Zeng,Chunmiao Cui,Haoyuan Weng,Yue Guo,Xiaoguang Liu,Ling Li
This study successfully synthesized Na 5 Y(WO 4 ) 4 doped with Sm³⁺, Pr³⁺, and Tb³⁺ using a high-temperature solid-state method. Photoluminescence spectra revealed that Na 5 Y(WO 4 ) 4 :Sm³⁺ phosphors emits a strong red glow at 646 nm under the excitation of 405 nm, and has an extremely high quantum yield, suggesting their suitability for plant growth LED lighting applications. In contrast, Na 5 Y(WO 4 ) 4 :Pr³⁺ phosphors displayed a lower efficiency but still aligned well with the red-wavelength absorption spectra of plants. Additionally, Na 5 Y(WO 4 ) 4 :Tb³⁺ phosphors demonstrated strong green emission under UV light, indicating potential applications in fingerprint detection. The Site Occupancy Theory (SOT) was utilized to explain the preferential occupation of dopant ions, showing that Sm³⁺ and Tb³⁺ preferred Y1 sites, whereas Pr³⁺ favored Na2 sites.
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