Food Analytical Methods
·
2024
·Vol. 17
·期 4
·585-593
·
IF 2.6
Wang Haoyu,Zhang Li,Chen Chen,Waterhouse Geoffrey I. N.,Sun Yufeng,Xu Zhixiang
In this study, a sensitive surface-enhanced Raman scattering (SERS) sensor based on core–shell-structured Au@Ag nanoparticles (NPs) was successfully developed for the detection of acrylamide (AA) in foods. Coating Au NPs with a shell of silver gave the Au@Ag NPs outstanding SERS performance for AA detection. Under the optimal experimental conditions, a good linear relationship was established SERS intensity at 1272 cm −1 and the logarithm of the AA concentration (over the concentration range 10 −8 ‒10 −3 mol L −1 ). The limit of detection (LOD) of the SERS method was 1.27 × 10 −9 mol L −1 . The recoveries of AA in spiked cookie samples were in the range of 85.68–102.50%. In addition, the SERS sensor was used to determine the content of AA in three food samples (potato chips, fried dough twist, and instant coffee), with the results obtained being consistent with those determined by high-performance liquid chromatography (HPLC), demonstrating that the SERS sensor was suitable for the detection of AA in real food samples.
研究领域:
分析化学
·
纳米技术
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食品科学
·
光谱学
✓ 已验证
DOI
高影响因子
JOURNAL OF PHYSICS AND CHEMISTRY OF SOLIDS
·
2019
·
IF 2.752
Hongji Li,Yan Wang,Yue Li,Jinyue Zhang,Yu Qiao,Qingwei Wang,Guangbo Che
As a sensitive analytical technique, surface-enhanced Raman scattering (SERS) is widely used in spectral analysis, single molecule detection and biological/chemical sensors. Noble-metal nanostructures have sensitive SERS properties and usually act as SERS substrates. Moreover, semiconductors also possess SERS properties and when the semiconductors are combined with noble metals this can not only enhance the SERS signal but also introduce additional properties. Therefore, in this investigation, SiO 2 @TiO 2 @Ag composite materials are selected as SERS substrates, which not only increase the SERS property of substrates but also introduce the property of self-cleaning under conditions of UV light . The molecularly imprinted technique (MIT) is combined with SERS in this investigation to improve the disadvantage of traditional SERS substrates having low selectivity . Molecularly imprinted polymers (MIPs) have specific adsorption properties that can enrich the templates on the surface of SiO 2 @TiO 2 @Ag composites. Importantly, the MIPs can prevent the SERS substrate being oxidized, which effectively protects the core materials. Hence, SiO 2 @TiO 2 @Ag@MIPs is synthesized based on SERS and MIT for used in pyrethroid detection. The Raman intensity and fenvalerate concentration show a good linear relationship in the FE range of 1.0–100 nmol l −1 and the detection limit is 0.2 nmol l −1 . Furthermore, the SiO 2 @TiO 2 @Ag@MIPs present good selectivity and self-cleaning. This investigation provides a novel approach to pyrethroid detection.
研究领域:
分析化学
·
环境科学
·
材料科学
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Xiang-Cheng Lin,Miaomiao Hu,Minzhe Li,Jian Liu,Qinjie Kou,Rong Huang,Lixian Sun,Changchun Wen
The molecular diagnosis of diseases through the highly sensitive and specific detection of very small amounts of biomarkers in biological samples remains a great challenge, and powerful detection strategies are urgently needed for basic research and clinical applications. Herein, a novel synergistic signal amplification strategy based on Au/Fe 3 O 4 /POPs to integrate magnetic porous composite Fe 3 O 4 /POPs with strong adsorption capabilities for target molecules and gold nanoparticles (AuNPs) with plasmonic properties, is proposed for ultra-high sensitivity and specific Surface-enhanced Raman spectroscopy (SERS) detection of the gastric cancer marker 4-hydroxybenzoic acid (4-HBA). The Au/Fe 3 O 4 /POPs-based SERS assay for gastric cancer-related biomarkers achieved significantly enhanced SERS signals arising from multiple signal amplifications, had good linear calibration from 10 −11 to 10 −5 mol/L, and could detect 4-HBA with a limit of detection (LOD) as low as 1.52 × 10 −12 mol/L. The detection of 4-HBA doped serum exhibited good specificity, homogeneity, and reliability. This material is easy to synthesize and simplifies the sample pretreatment process. This innovative SERS substrate is promising for the rapid detection of disease markers, particularly for early diagnosis and monitoring the effectiveness of gastric cancer treatments.
研究领域:
分析化学
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生物医学
·
纳米材料科学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Chemistry
·
2024
·
IF 3.8
Tian Yongfeng,Zhao Lu,Shen Xiaofeng,Shang Shanzhai,Pan Yonghua,Dong Gaofeng,Huo Wang,Zhu Donglai,Tang Xianghu
Surface enhanced Raman spectroscopy (SERS) is a unique analytical technique with excellent performance in terms of sensitivity, non-destructive detection and resolution. However, due to the randomness and poor repeatability of hot spot distribution, SERS quantitative analysis is still challenging. Meanwhile, snus is a type of tobacco product that can release nicotine and other components in the mouth without burning, and the rapid detection technique based on SERS can reliably evaluate the amount of nicotine released from snus, which is of great significance for understanding its characteristics and regulating its components. Herein, the strategy was proposed to solve the feasibility of SERS quantitative detection based on self-assembled core-shell nanoparticles with embedded internal standards (EIS) due to EIS signal can effectively correct SERS signal fluctuations caused by different aggregation states and measurement conditions, thus allowing reliable quantitative SERS analysis of targets with different surface affinity. By means of process control, after the Au nanoparticles (Au NPs) were modified with 4-Mercaptobenzonitrile (4-MBN) as internal standard molecules, Ag shell with a certain thickness was grown on the surface of the AuNP@4-MBN, and then the Au@4-MBN@Ag NPs were used to regulate and control the assembly of liquid-liquid interface. The high-density nano-arrays assembled at the liquid-liquid interface ensure high reproducibility as SERS substrates, and which could be used for SERS detection of nicotine released from snus products. In addition, time-mapping research shows that this method can also be used to dynamically monitor the release of nicotine. Moreover, such destruction-free evaluation of the release of nicotine from snus products opens up new perspectives for further research about the impact of nicotinoids-related health programs.
研究领域:
分析化学
·
烟草研究
·
健康科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Small
·
2024
·Vol. 21
·期 6
·2409806
·
IF 13
Ruiyuan Zhang,Yu Guo,Chen Huang,Jixiang Fang
The heterogeneity of extracellular vesicles (EVs) surface information represents different functions, which is neglected in previous studies. In this study, a label-free SERS analysis approach is demonstrated to study fundamental EV biological and physical information heterogeneity by matching specific sizes of nano-enhanced particles. This strategy reveals informative, comprehensive, and high-quality SERS spectra of the overall exosome surface, and effectively circumvents the key information loss caused by the spatial resistance of NPs binding to the 293 exosomes' concave structure. The classification of normal and cancerous cell-derived exosomes by PCA method, the accuracy is improved from 91.2% to 95.1% by optimizing sizes of nano-enhanced particles. In addition, stem cell-derived EVs of diverse sizes and morphologies similarly show acuity of spectrum variation to NPs size, which is conductive to qualitative studies. This new strategy will offer a widened in-depth understanding of the surface information, size, and morphology of EVs, which can be applied to the study of biological functions.
研究领域:
生物医学工程
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纳米生物学
·
癌症研究
✓ 已验证
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Panxue Wang,Ying Liu,Xiang Li,Cen Li,Guoliang Li
Rapid and reliable determination of pathogenic bacteria is crucial for food safety and public health. Surface-enhanced Raman spectroscopy (SERS) based aptasensors have attracted increasing attention in the determination of pathogenic bacteria due to their high specificity and sensitivity. However, the sensitivity and reliability of the SERS aptasensors for the determination of pathogens were hindered by the uneven distribution of SERS tags. To tackle this limitation, a SERS aptasensor based on a hybridization chain reaction (HCR) mediated signal conversion strategy was fabricated for the determination of E. coli O157:H7. In the presence of E. coli O157:H7, the blocked aptamer specifically binds with it and releases the blocker DNA. Then the blocker DNA hybridizes with the capture DNA on the SERS probe and initiates the HCR reaction, which further leads to the formation of double stranded DNA and the reduction of the SERS signal intensity of 4-mercaptobenzonitrile (4-MBN). Under the optimal conditions, the SERS signal intensity at 1073.9 cm −1 and the logarithmic value of the concentration of E. coli O157:H7 in the range of 8 × 10 2 –8 × 10 7 CFU·mL −1 exhibited a good linear relationship, with a coefficient of determination of 0.92. The limit of detection for E. coli O157:H7 was 409 CFU·mL −1 . Besides, this aptasensor exhibited satisfactory specificity for E. coli O157:H7. In addition, it showed excellent stability in real sample analysis with relative standard deviations (RSDs) below 3.76 % (n = 3). The established SERS aptasensor employed a HCR mediated signal conversion strategy and overcame the drawback of unevenly distribution of SERS tags in classical SERS aptasensors for the determination of pathogenic bacteria. This study offered a new reference for the rapid and reliable detection of pathogens with a portable Raman spectrometer.
研究领域:
食品安全
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生物分析化学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Xinyue Wang,Chen Chen,Geoffrey I.N. Waterhouse,Xuguang Qiao,Yufeng Sun,Zhixiang Xu
Herein, a novel surface enhanced Raman spectroscopy (SERS) aptasensor was developed for amantadine (AMD) detection, based on magnetite nanoparticles coated with polyethylenimine, silver nanoclusters and aptamers (Fe 3 O 4 @PEI@AgNC-apt) as the capture probe and complementary DNA-modified gold nanorods (AuNRs@4-MPBA@Ag-c-DNA containing 4-mercaptophenylboric acid molecules) as the reporter probe. In the presence of AMD, the AMD and the reporter probe competed for the aptamer on the surface of the capture probe, resulting in the reporter probe detaching from the capture probe leading to a decrease in intensity of the SERS signal at 1067 cm −1 for 4-MPBA. Under optimal conditions, a good linear relationship was established between the SERS intensity at 1067 cm −1 and the logarithm of the AMD concentration over the range 10 −6 -10 2 mg L −1 , with a LOD of 0.50 × 10 −6 mg L −1 . The AMD levels in spiked samples were evaluated using the SERS aptasensor, with good recoveries ranging from 90.57% to 113.49% being obtained.
研究领域:
分析化学
·
纳米技术
·
食品科学
·
光谱学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2024
·
IF 4.9
Yu-fang Fu,Jian Zhu,Xin Li,Guo-jun Weng,Jian-jun Li,Jun-wu Zhao
In this work, we synthesized Au-Ag alloy nano-garlands with multiple petal-like branches and internal cavities in two steps. First, we etched silver nanospheres with HAuCl 4 to obtain Au-Ag alloy nanoshells with different shell layer thicknesses and hollow core sizes. After that, based on different nanoshells, 4-NTP was used as a shape-directing agent, HAuCl 4 and AgNO 3 as precursor raw materials, and AA as a reducing agent for the growth of petals to form Au-Ag bimetallic nano-garlands with multi-branching structure. The regulation of the branching morphology of the nano-garlands could be achieved by changing the amounts of 4-NTP and HAuCl 4 . The SERS activity of the nano-garlands could be further improved by changing the AA concentration, the size of nanoshells, and the Au/Ag ratio of the precursor raw materials. Because the bimetallic nano-garlands have hollow cavities and petal-like branches, there are couplings between the cavities and the branches, between the adjacent branches, and between the metals inside and outside of the cores, which can achieve a good enhancement of SERS. The highest SERS enhancement factor of Au-Ag bimetallic nano-garlands can reach 4.47×10 8 , which can be used as a good candidate for SERS substrate for the trace detection of biomolecules , pesticides, and other substances.
研究领域:
材料科学
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纳米技术
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN
·
2021
·Vol. 94
·期 3
·860-868
·
IF 5.488
He Yuhan,Li Lijun,Li Xiaoxuan,Lin Chubing,Zhang Yan
According to its characteristics, borate can recognize and adsorb target glycoproteins and specific binding properties of antigens and antibodies. This article introduces a preparation method for a magnetic immune SERS sensor, and it is used for the specific identification and detection of trace AFP in human serum. The test used AFP as the target protein and 4-mercaptophenylboronic acid (4-MPBA) modified gold-coated silver nanoparticles (Ag@AuNPs) as the signal factor. After the AFP antibody was grafted on the boric acid group, the SERS detection probe (Ag@Au@4-MPBA@Anti-AFP) was obtained. On the surface of boric acid-functionalized (-P) magnetic silica particles, AFP antibody was modified, and the particles were used as magnetic immunocapture probes (Fe3O4@SiO2-P@Anti-AFP). Finally, the sandwich structure complex of “detection probe-target protein-capture probe” was successfully constructed. The prepared magnetic immune SERS sensor exhibits excellent selectivity and high sensitivity for AFP detection; based on the signal-to-noise ratio S/N = 3, the detection limit can reach 1.0 ng/mL; by performing a spike test on AFP in human serum, the sensor can be used to detect AFP in actual samples. The recovery rate was between 85.8% and 105.7%, and the RSD distribution was ≤3.52%. The sensor was easy to prepare, required no complex processing of samples and can achieve rapid detection, so that it is expected to become a new strategy for clinical detection of AFP.
研究领域:
生物化学
·
医学检测
·
纳米技术
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Yujie Pan,Peini Zhao,Weili Cai,Hehe Fang,Xiaoru Wang,Junyu Zhao,Peihua Zhu,Hongmei Yang,Shenguang Ge,Jinghua Yu
Carbaryl is a broad-spectrum carbamate fungicide that may pose a threat to ecosystems and human health. To prevent and control the harm caused by excessive application of carbaryl, a full-dimensional divergence effect SERS sensor has been constructed. Biodegradable paper chips were used as sensor substrates. ZIF-8 with structural flexibility was grown in situ on carbon paper. A rough silver layer with surface like metal etching was clad outside the ZIF-8. Artificial antibody (AAB) with accurate grasping ability was polymerized on the surface of ZIF-8@Ag paper substrate. Au@Ag CSNPs were decorated on the AAB to provide abundant “hot spots”. Every functional material layer works together to form a gap and tip region with superior plasmon resonance. The proposed SERS sensor realizes the rapid and accurate on-site screening and localization of carbaryl in food with good correlation responded in the range of 0.01–20 μg·mL −1 and a low detection limit of 5.72 × 10 –3 μ g·mL −1 .
研究领域:
分析化学
·
环境科学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Jinai Chen,Yujiao Wei,Liming Wang,Leyi Chen,Shengnan He,Honglin Liu
We present a digital surface-enhanced Raman spectroscopy (SERS) platform enabling enzyme- and amplification-free single-molecule DNA detection through Poisson distribution-driven quantification and geometrically optimized plasmonic nanostructures. Utilizing high-throughput UV lithography, we fabricated large-area nanopillar arrays (1.5 × 1.5 cm) with tunable heights (500–1300 nm) and periodicities (4–10 μm), addressing key limitations in conventional SERS methods via three innovations: (1) dynamic hotspot generation: Target DNA induces plasmonic dimerization between functionalized gold nanoprobes and Au-capped nanopillars, creating spatially resolved SERS enhancement (1326 cm–1 DTNB signal), (2) digital counting algorithms: Compensate Raman intensity variations by quantifying activated pillars as discrete molecular events, and (3) geometric confinement: Spatial confinement effects enabling statistically robust single-molecule occupancy. This platform achieved 10 fM sensitivity for Diaporthe spp. DNA detection, outperforming PCR-electrophoresis (1 pM) by 2 orders of magnitude. Applied to presymptomatic kiwifruit diagnosis, the system identified pathogen DNA in asymptomatic samples prior to visual symptom development, validated by characteristic DTNB peaks (1326 cm–1) absent in negative controls. The modular design permits multiplexed detection through probe diversification, while the absence of enzymatic steps enables field-deployable operation. By integrating single-molecule statistics with plasmonic signal amplification, this work establishes a robust framework for precision molecular diagnostics, demonstrating transformative potential for agricultural pathogen surveillance and low-abundance biomarker analysis. The technology’s batch-producible nanopillar architecture and digital quantification strategy overcome longstanding reproducibility challenges in SERS-based detection, positioning it as a versatile tool for next-generation biosensing applications.
研究领域:
农业科学
·
分子诊断学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Photonics
·
2024
·Vol. 11
·期 8
·708
·
IF 2.1
Mingzhong Zhang,Shanshan Xu,Peng-Cheng Guan,Yue-Jiao Zhang,Jian-Feng Li
In surface-enhanced Raman scattering (SERS) detection research, the shape, structure, surface modification, and material selection of nanoparticles can significantly impact the SERS intensity. Petal-like gap-enhanced Raman tags (P-GERTs) possess numerous sharp tips and edges, which generate localized electric field enhancements, further amplifying the electric field enhancement effect on neighboring molecules and enhancing the SERS signal. Additionally, the surface of P-GERTs can be modified with functional molecules, enabling their application in the detection of disease biomarkers. Using COVID-19 as an example, the performance of P-GERTs in disease biomarker detection was validated, demonstrating that the signal intensity of this probe can reach 55 times that of regular gold nanoparticles and 36.7 times that of smooth shell gap-enhanced Raman tags (S-GERTs). Furthermore, in combination with magnetically retrievable magnetic bead substrates, the N-protein antigen was specifically detected in a one-step process. N-protein was detected within 15 min using a portable Raman spectrometer. The limit of detection (LOD) for the standard sample was 4.28 pg/mL, and the LOD for the actual throat swab sample system was 25.4 pg/mL. This workflow can be extended to the detection of other biomarkers, making it widely applicable.
研究领域:
分析化学
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生物医学工程
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纳米技术
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分子生物学
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诊断医学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）