SENSORS AND ACTUATORS B-CHEMICAL
·
2026
·
IF 8.3
Panyi Hu,Liping Su,Jinfeng Liu,Rui Zhao,Dong Wang,Jiahan Cheng,Xiaohe Tian
Reliable visualization of lipid droplet (LD) remodeling remains challenging because conventional LD probes often suffer from background fluorescence and limited photostability in lipid-rich cellular environments. Here, we developed LD-TTP, an LD-targeting fluorescent probe integrating aggregation-induced emission (AIE) and intramolecular charge transfer (ICT) properties. LD-TTP exhibits strong fluorescence activation, high selectivity, and excellent photostability, enabling robust imaging and quantitative analysis of LD dynamics in cell-based steatotic models. Using staged steatotic HepG2 cells, we found that LD remodeling was accompanied by stage-dependent changes in the spatial distribution of the amyloid-β (Aβ)-associated receptor low-density lipoprotein receptor–related protein 1 (LRP1). In early steatosis, LRP1 expression increased and became enriched around LDs, coinciding with enhanced uptake of exogenous Cy5-labeled Aβ. As steatosis progressed, LRP1 expression declined, and its LD-associated localization was lost, paralleling reduced Aβ uptake. A diacylglycerol acyltransferase 1 (DGAT1) inhibitor partially restored Aβ uptake in moderate-to-severe steatotic HepG2 cells. These findings provide cellular-level evidence for a stage-dependent relationship among LD remodeling, LRP1 alteration, and Aβ uptake in hepatocytes. Although the present results offer mechanistic clues at the cellular level, they do not fully capture the influence of the physiological microenvironment in vivo . Further animal studies will be required to determine whether these LD–LRP1–Aβ-associated changes are maintained under physiological conditions.
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高影响因子
Journal of Translational Medicine
·
2026
·Vol. 24
·期 1
·539
·
IF 9.7
Gao Yuan-Yuan,Sun Wen-Shuang,Sun Zi-Ying,Wang Jun-Rui,Lv Zhong-Yang,Li Yu-Jia,Tian Hao-Yuan,Bao Zheng-Yang,Qiu Xin-Ran,Wang Zheng,Zheng Shi-Zhong,Shao Jiang-Juan,Zhang Zi-Li,Meng Jia
Background Tendinopathy is a prevalent orthopaedic condition characterized by disrupted tendon homeostasis, with oxidative stress being a key contributing mechanism. Although the natural compound 18-β-Glycyrrhetinic acid (GA) exhibits antioxidant properties and is a therapeutic candidate for tendinopathy, its precise molecular mechanism remains unclear. This study aimed to elucidate how GA alleviates tendinopathy, with a focus on its role in regulating the HMGB1-cGAS-STING axis and NLRP3 inflammasome activation in the context of oxidative stress. Methods We employed single-cell RNA sequencing (scRNA-seq) of clinical samples, proteomics of animal tissues, and comprehensive pharmacological assays to investigate the mechanisms of tendinopathy. Furthermore, the rat tendinopathy model and H 2 O 2 -induced oxidative stress model of tendon stem cells (TSCs) were used to validate the protective effects of GA. Results We found that GA significantly reduced oxidative stress and subsequent inflammation, thereby mitigating collagen disruption in rats with tendinopathy. Notably, scRNA-seq revealed that the proportion of TSCs increased significantly during tendinopathy, which were particularly susceptible to reactive oxygen species (ROS). TSCs from oxidative damage and inhibited activation of the NLRP3 inflammasome by suppressing the cGAS-STING pathway. Mechanistically, GA promoted cGAS degradation by enhancing its interaction with the mitochondrial E3 ubiquitin ligase Mul1. This effect was mediated through high-mobility group box 1 (HMGB1), as GA disrupted the HMGB1-cGAS interaction. Specifically, GA induced methylation of HMGB1 at lysine 43, a modification essential for its activity. This methylation was catalyzed by the methyltransferase DOT1L, which was upregulated and directly bound by GA. Collectively, GA alleviates tendinopathy by targeting the DOT1L-HMGB1-cGAS axis to resolve oxidative stress and inflammation. Conclusion Collectively, our findings provide new insights into how oxidative stress accelerates tendinopathy progression. Moreover, they delineate the mechanism by which GA in mitigates oxidative damage and inflammation in TSCs by inhibiting the co-localization of HMGB1 and cGAS. Overall, this study offers scientific support for further developing GA as a promising therapeutic agent for tendinopathy treatment. Graphical The alternative text for this image may have been generated using AI.
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高影响因子
Stem Cell Research & Therapy
·
2026
·Vol. 17
·期 1
·200
·
IF 7.8
Chen Qi,Chen Xiaolong,Yang Qu,Miao Xinxin,Yuan Jinghong,Ding Shuihua,Ding Rui,Cai Shenghao,Li Bin,Cheng Xigao
Background Intervertebral disc degeneration (IVDD) is characterized by progressive nucleus pulposus cell loss and extracellular matrix degradation, in which persistent oxidative stress plays a critical pathogenic role. Transplantation of nucleus pulposus–derived stem cells (NPSCs) is a promising therapeutic strategy, yet the hostile oxidative microenvironment severely compromises cell survival. Although cellular quiescence has been suggested to enhance stress tolerance, its regulatory mechanisms and relevance in NPSCs remain largely unexplored. Methods Oxidative stress was evaluated in human degenerated disc tissues, a rat needle-puncture degeneration model, and tert-butyl hydroperoxide–treated NPSCs in vitro. Proliferating and quiescent NPSCs were compared for reactive oxygen species (ROS) levels, apoptosis, viability, and transcriptomic profiles. Pathway enrichment analyses were performed to identify critical signaling mechanisms. Recombinant transforming growth factor-beta 3 (rhTGF-β3) was used to activate the pathway, while small interfering RNA targeting the transforming growth factor-beta receptor type 2 ( Tgfbr2 ) and the pharmacological inhibitor SB431542 were applied for pathway suppression. Functional assays, organ culture, and in vivo transplantation were conducted to assess cell survival and regenerative effects. Results Elevated oxidative stress was consistently observed across clinical, animal, and cellular models of disc degeneration. Quiescent NPSCs demonstrated enhanced resistance to oxidative injury, with reduced ROS accumulation, decreased apoptosis, and improved survival. Transcriptomic analyses revealed suppression of metabolic and P53-mediated apoptotic pathways, alongside marked activation of TGF-β/SMAD signaling. Activation of this pathway induced quiescence, reduced ROS levels, inhibited mitochondrial apoptotic signaling, and protected NPSCs from oxidative injury, whereas pathway inhibition abolished these protective effects. In both organ culture and in vivo transplantation models, quiescent and TGF-β–activated NPSCs exhibited superior survival and significantly improved disc structural preservation compared with proliferating or pathway-blocked cells. Conclusion Activation of the TGF-β/SMAD pathway induces NPSC quiescence and enhances oxidative stress tolerance by suppressing P53-dependent mitochondrial apoptosis. Pharmacological induction of quiescence represents a potential strategy to improve stem cell–based therapies for intervertebral disc degeneration.
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高影响因子
MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Yanying Rao,Binggui Wang,Hong Li,Xiaoying Ding,Kaiguo Liu,Xiaojuan Wang,Yue Li
Integrating β-CD-AgNPs and AuNSs arrays enables rapid, quantitative SERS sensing of antibiotic residues. • The system detects AMP, CIP, ENR, NFX individually and in mixtures with high sensitivity. • The combined substrate enabled accurate, applicable antibiotic detection in real waters. β-Cyclodextrin-functionalized Ag nanoparticles (β-CD-AgNPs) were synthesized via a one-step method and integrated with ordered Au nanoshells (AuNSs) arrays to construct a highly sensitive surface-enhanced Raman scattering (SERS) sensor for antibiotic detection. The specific dimensions of the β-CD-AgNPs enable selective binding, drawing antibiotic molecules into close proximity with the AgNPs surface. This proximity generates plasmonic hot spots, significantly amplifying the Raman signals. In this design, the β-CD-AgNPs serve a dual role as both the primary SERS substrate and an internal standard. A strong linear relationship was established between the concentration of antibiotics and the intensity ratio of the characteristic antibiotic peak to the β-CD-AgNPs peak. Based on this principle, we achieved sensitive detection of both individual antibiotics and their mixtures, and validated the method through spike-and-recovery experiments in environmental water samples. The calibration plots were established by linearly fitting the intensity ratio (antibiotic characteristic peak/internal standard peak) against the concentration of Ampicillin (AMP), Ciprofloxacin (CIP), Enrofloxacin (ENR), and Norfloxacin (NFX) solutions, all exhibiting excellent linearity (R 2 > 0.99), as well as for binary mixtures of CIP and AMP, with R 2 values of 0.9942 and 0.9953, respectively. The limits of detection (LODs) for these antibiotics ranged from 0.0827 to 0.3365 μmol·L −1 , and the limits of quantification (LOQs) ranged from 0.27 to 1.11 μmol·L −1 . The linear working ranges were 0.61–5.00, 0.55–12.00, 0.48–12.00, and 1.11–12.00 μmol·L −1 for AMP, CIP, ENR, and NFX, respectively. In environmental water samples, apparent recovery rates of individual antibiotics ranged from 98.84% (±0.03%) to 100.02% (±0.01%), with relative standard deviations (RSDs) between 1.32% and 4.92%. For CIP and AMP mixtures in similar samples, recoveries ranged from 98.53% (±0.01%) to 111.29% (±0.04%), with RSDs between 1.15% and 5.71%. These results demonstrate that the proposed SERS sensor enables efficient and accurate antibiotic detection, offering a viable approach for the rapid and highly sensitive monitoring of antibiotic contaminants in the environment. Download: Download high-res image (188KB) Download: Download full-size image
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产品关联
DOI
高影响因子
Small
·
2025
·
IF 12.1
Hao Wu,Jinyi Pan,Chao Wu,Jie Wang,Chunyi Jiang,Chenyu Guo,Zhihao Yu,Zhenyang Wang,Sichao Du,Daoyou Guo,Jun Hu
Solar-blind photodetection plays a crucial role in environmental monitoring, corona detection, and covert battlefield communication due to its unique high signal-to-noise ratios in the UVC band. Aluminum quantum dots (AlQDs) emerge as promising optical materials owing to their extended photoresponse in the ultraviolet region, along with their low cost and compatibility with optoelectronic devices. Herein, an AlQDs-based cascade solar-blind photodetector is presented with enhanced sensitivity, achieved through the quantum confinement effect. By employing a gradient double-layer AlQDs stack integrated with β -Ga₂O₃, the heterojunction demonstrates a significantly suppressed dark current, reduced from 3 nA to 2 pA, and a spectral noise density of 2.8 × 10 −11 A Hz⁻ 1 / 2 compared to the single-layer device. The optimized photodetector achieves a high UV–vis rejection ratio ( R 250 / R 400 ) of 2.5 × 10 3 , a specific detectivity of ≈2.3 × 10 1 2 Jones, a relatively fast response time of 25 ms, and a responsivity of 35.1 mA W −1 under a 1 V bias. Furthermore, the device demonstrates robust interference-resistant imaging capabilities, enabling ultra-weak photodetection down to 23 nW cm −2 . These results highlight the potential of AlQDs-based cascade devices for advanced solar-blind photodetection applications.
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Xinying Peng,Yaoyao Ji,Kuo Zhao,Xue Shen
High internal phase Pickering emulsions (HIPPEs) exhibit great potential in 3D printing and the delivery of hydrophobic compounds due to their semi-solid rheological properties and high oil phase volume. In this study, quinoa protein-saccharide conjugates were used to stabilize HIPPEs as 3D printing inks for the delivery of β-carotene. The quinoa protein-inulin conjugate (QP-Inu) demonstrated a larger three-phase angle approaching 90°, lower interfacial tension, and a higher absorption rate, resulting in a compact network structure with more tightly packed smaller droplets, as confirmed by particle size and confocal laser scanning microscopy (CLSM) analysis. All HIPPEs exhibited solid-like viscoelasticity and shear-thinning behavior. Among these, QP-Inu stabilized HIPPEs displayed higher viscosity and elasticity, superior thixotropic recovery rates, and enhanced 3D printability with improved accuracy and self-supporting ability. Furthermore, QP-Inu stabilized HIPPEs showed a better encapsulation capacity for β-carotene and superior storage ability, and stability against adverse environmental stresses, including pH, heating, salt ions, and oxidation. In conclusion, quinoa protein-saccharide conjugates can be promising candidates as stabilizers to form food-grade HIPPEs as new 3D printing materials and effective β-carotene delivery system.
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL RESEARCH
·
2025
·
IF 7.7
Zhongyuan Zhang,Jiaming Fu,Yihong Di,Honghui Li,Bing Wu,Xueyan Tian,Meiyan Li,Yuqing Dai,Zhuoheng Shen,Jinhao Jia,Zeyang Bai,Limeng Xiong,Yuhan Zhang,Xiaoyu Li,Yi Zhao,Hao Hu,GuangJun Wang,Huifang Yang,Rui Zhang,Jian Sun
Previous epidemiologic studies have indicated a positive association between population-based imidacloprid (IMI) exposure and diabetes. Moreover, toxicological evidence suggests that IMI can disrupt glucose metabolism in non-target organisms. However, the toxic doses of IMI in previous experimental studies were much higher than the exposure levels in the real world, and the underlying mechanism remains unknown. In this study, based on the exposure level of neonicotinoids in humans, we investigated the possible mechanism of IMI-induced glucose metabolism disorders using cybertoxicology predictions, a long-term exposure model of IMI in mice, and cellular models. The cybertoxicology predictions showed that NF-κB and apoptosis play key roles in the association of IMI, pancreatic β-cell injury, and diabetes. However, exposure of mice to IMI through potable water (104 μg/kg, population exposure maximum) for 24 weeks did not result in abnormal glucose metabolism (P > 0.05), while significant activation of inflammatory response, NF-κB phosphorylation, and mitochondrial apoptosis pathways were observed (P < 0.05). After inhibition of NF-κB P65 phosphorylation in the cellular model, IMI-induced alterations in the MIN6 cell structure and function, inflammatory responses, and mitochondrial apoptotic pathways were alleviated (P < 0.05). This finding provides evidence for the disruption of glucose metabolism due to long-term exposure to IMI, and subsequent studies should focus on the effects of longer exposure to IMI on glucose metabolism as well as the role of the NF-κB signaling pathway.
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2026
·
IF 2.6
Ningbo Li,Yongdong Wei,Mengsi Liang,Wenkun Zhang,Jie Qiao
Given that ochratoxin A (OTA) exhibits immunotoxic, teratogenic, and carcinogenic properties, it is essential to monitor its presence in food samples. In the present work, a novel electrochemical aptasensor for OTA detection uses β-cyclodextrin-functionalized graphene (CG) to enhance electron transfer and provide abundant double-stranded DNA (dsDNA) binding sites. The sensor immobilizes OTA aptamers, complementary DNA (cDNA), and ferrocene-labeled dsDNA on a gold nanoparticle-modified electrode. The host–guest interaction between β-cyclodextrin (β-CD) and ferrocene (Fc) stabilizes the sensing layer, while thiolated dsDNA ensures strong attachment to AuNPs. Without OTA, impedance is high and current is low. In the presence of OTA, its high affinity for the aptamer induces conformational changes in dsDNA. Upon applying an oxidative potential, ferrocene is oxidized to Fc+, which, due to reduced hydrophobicity, dissociates from β-CD. This releases dsDNA from the electrode surface, reducing the impedance and enhancing the current response. The sensor demonstrates a robust response across a concentration range of 1 pg mL−1 to 100 ng mL−1, achieving a detection limit of 0.78 pg mL−1. Notably, this method has been successfully applied to detect OTA in wine, yielding high recovery rates of 96.20% to 98.67%. These findings suggest that the MCH/AuNPs/dsDNA/CG/GCE configuration is a promising electrochemical sensor for OTA detection.
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产品关联
DOI
PubMed
高影响因子
Journal of Nanomaterials
·
2021
·Vol. 2021
·期 1
·4145010
·
IF 2.986
Jin Xu,Xuecheng Sun,Xiaodong Sun,Ye Lu
In this paper, a capillary coated with poly(glycidyl methacrylate) nanoparticles (PGMA NPs) was prepared and applied to construct a capillary electrophoresis (CE) enantioseparation system with glucosyl- β -cyclodextrin (Glu- β -CD) as a chiral selector. The PGMA NP coating can be easily introduced through a simple ring-opening reaction. Two basic drugs were used as models to evaluate the separation performance of the PGMA coating. Under the optimal conditions selected, the separation of the two enantiomers was obtained.
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高影响因子
Food Analytical Methods
·
2013
·Vol. 7
·期 6
·1256-1262
·
IF 1.969
Zhou Nan,Sang Renhao,Zhu XiaShi
Functionalized β-cyclodextrin polymer, mono-6-deoxy-6-imidazole-β-cyclodextrin polymer (β-CDIMCP), was synthesized as a new solid phase adsorbent coupled with UV–visible spectroscopy to separate/analyze trace kaempferol. The results showed that kaempferol was adsorbed rapidly by β-CDIMCP (adsorption efficiency, 94.7 %) and then eluted by methanol (elution efficiency, 93.4 %). Under the optimum conditions, the calibration curve was linear in the concentration range from 0.10 to 14.0 μg/mL, with a correlation coefficient ( R ) of 0.9965. And the limit of detection was 0.028 μg/mL for kaempferol. The preconcentration factor of the method was tenfold. The molecular interaction between β-CDIMCP and kaempferol was studied through inclusion constant and FTIR analysis. The present method had been successfully applied to determine kaempferol in red wine samples.
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高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2018
·
IF 3.412
Yumeng Zhang,Yuanqi Lv,Lin Chen,Haizhou Wu,Yingyang Zhang,Zhiyao Suo,Shuxin Wang,Yuxin Liang,Xinglian Xu,Guanghong Zhou,Xianchao Feng
Nowadays, natural antioxidants abundant in polyphenols have been widely used to substitute synthetic antioxidants in meat products. In general, high doses of natural antioxidants are required to provide comparative antioxidant effects as synthetic antioxidants. Noticeably, the qualities of meat products can be jeopardized due to interactions between polyphenols and myofibrillar proteins (MPs). In this study, methyl-β-cyclodextrin was used to increase the polyphenol loading amount by preventing interactions between polyphenols and proteins. Solubility, electrophoresis, fluorescence spectroscopy, and surface hydrophobicity analyses indicated that methyl-β-cyclodextrin could dose-dependently inhibit epigallocatechin-3-gallate-induced attacks on MPs under oxidative stress. Gel strength, cooking loss, confocal laser scanning microscopy, dynamic rheological testing, and Raman spectrum during gelation were further analyzed to investigate the effects of methyl-β-cyclodextrin on the qualities of epigallocatechin-3-gallate-treated emulsion gel. Methyl-β-cyclodextrin addition prevented modification of the secondary structure of MPs caused by epigallocatechin-3-gallate. In consequence, the gel and emulsifying properties of MPs were significantly improved. Moreover, β-cyclodextrins could partly inhibit oxidative attacks on MPs and thus increase their solubility. These results indicated that methyl-β-cyclodextrin addition effectively enhanced epigallocatechin-3-gallate loading capacity in meat products.
研究领域:
食品科学与技术
·
蛋白质化学
·
多酚-蛋白质相互作用
·
氧化稳定性
·
功能食品
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DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2022
·
IF 5.518
Ben Chen,Tingting Lin,Haibo You,Liqun Fang,Chu Chu,Jintao Yang,Shengqiang Tong
The surface of SiO 2 coated magnetic nanoparticles (NMPs) was modified by bonding with sulfobutylether- β -cyclodextrin (SBE- β -CD) via carbodiimide activation for the first time. The magnetic nanoparticles were synthesized by chemical coprecipitation method under alkaline condition, and the magnetic particles were modified by citric acid . Then the surface of obtained magnetic nanoparticles was coated with SiO 2, and SBE- β -CD modified by citric acid was bonded to the surface of Fe 3 O 4 /SiO 2 MNPs under carbodiimide activation method. The synthesized functionalized magnetic nanoparticles were characterized by X-ray Diffraction, Fourier Transform Infra-Red spectroscopy, thermogravimetric analyses and transmission electron microscopy . These functional nano-sized particles were scrutinized for enantioselective liquid-liquid extraction of five racemic drugs/intermediates, including trans -paroxol, N-methyl duloxetine , fluoxetine , tropic acid and acetyltropic acid, and it was found that compared with traditional SBE- β -CD, the present magnetic silica nanoparticles bonded with SBE- β -CD showed higher enantiorecognition with some racemates using enantioselective liquid-liquid extractions. To our knowledge, this is the first-time report on preparation and application of magnetic nanoparticles bonded with SBE- β -CD in enantioselective liquid-liquid extraction for chiral separations .
研究领域:
纳米材料
·
分析化学
·
手性分离
·
磁性材料
·
超分子化学
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DOI
高影响因子
危险品化学品经营许可证（带存储）