BIOMEDICAL MICRODEVICES
·
2017
·Vol. 19
·期 2
·1-10
·
IF 2.062
Kang Yufeng,Zou Lili,Qiu Bensheng,Liang Xin,Sun Sijie,Gao Dayong,Ding Weiping
In this paper, a multistep dilution-filtration microdevice (MDFD) is developed for unloading cryoprotectants from cryoprotectant-loaded cells. The MDFD contained a diluent producing region, a dilution-filtration execution region, and a filtrate collection region. It was made of two patterned PMMA stamps with four pieces of sandwiched PVDF membranes. Firstly, the performances of the mixers that were used in the diluent producing region and the dilution-filtration execution region were assessed using fluorescence experiments. Then, the effect of the MDFD structure on the loss of cells was investigated by applying the MDFD to unload glycerin from glycerin-loaded porcine red blood cells. Finally, the effects of the cell density, glycerin concentration, and membrane pore size on the clearance efficiency of glycerin ( C
G
), the survival rate of cells ( S
C
) and the recovery rate of cells ( R
C
) have been studied. Under the designed conditions, C
G
achieved ~80% and S
C
reached ~90%. However, R
C
was only ~40%, mainly resulting from the cells detained on the membrane surface and squeezed through the membrane pores into the filtrate. Increasing the membrane pore size caused high C
G
and S
C
, but low R
C
. For a low glycerin concentration, C
G
, S
C
, and R
C
were all high. For a high cell density, C
G
was high, but both S
C
and R
C
were low. This work is of significance to develop a microfluidic chip for unloading cryoprotectants from a small amount of cryopreserved cell samples.
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Scientific Reports
·
2017
·Vol. 7
·期 1
·1-12
·
IF 4.259
Zhang Chong,Cao Songxiao,Jin Yazhong,Ju Lijun,Chen Qiang,Xing Qiaojuan,Qi Hongyan
To better understand the function role of the melon CmLOX18 gene in the biosynthesis of C6 volatiles during fruit ripening, we biochemically characterized CmLOX18 and identified its subcellular localization in transgenic tomato plants. Heterologous expression in yeast cells showed that the molecular weight of the CmLOX18 protein was identical to that predicted, and that this enzyme possesseed lipoxygenase activity. Linoleic acid was demonstrated to be the preferred substrate for the purified recombinant CmLOX18 protein, which exhibited optimal catalytic activity at pH 4.5 and 30 °C. Chromatogram analysis of the reaction product indicated that the CmLOX18 protein exhibited positional specificity, as evidenced by its release of only a C-13 oxidized product. Subcellular localization analysis by transient expression in Arabidopsis protoplasts showed that CmLOX18 was localized to non-chloroplast organelles. When the CmLOX18 gene was transgenically expressed in tomato via Agrobacterium tumefaciens -mediated transformation, it was shown to enhance expression levels of the tomato hydroperoxide lyase gene LeHPL , whereas the expression levels of six TomLox genes were little changed. Furthermore, transgenic tomato fruits exhibited increases in the content of the C6 volatiles, namely hexanal, (Z)-3-hexanal, and (Z)-3-hexen-1-ol, indicating that CmLOX18 probably plays an important role in the synthesis of C6 compounds in fruits.
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WATER AIR AND SOIL POLLUTION
·
2017
·Vol. 228
·期 6
·1-13
·
IF 1.702
Li Huiqin,Hu Jingtao,Wang Chuan,Wang Xiaojing
Chicken feather, which is consisted of keratin, has always been abandoned as solid waste. The utilization technologies of waste keratin have been developed in electric zones and materials fields so far. Recently, numerous new types of adsorbents have been used for antibiotic removal. The chicken feather carbon is supposed to be a potential one. In this study, an activated feather carbon (AFC) was developed as the absorbent of amoxicillin (AMOX) in simulated wastewater. The micropore structures of AFC were detected by the scanning electron microscope (SEM). X-ray photoelectron spectrum (XPS) was recorded and analyzed. A BET surface area, as high as 1838.86 m 2 /g, was measured in this study. At the meantime, a rapid adsorption (5∼7 min) and high removal efficiency (99.63%) could be observed. The kinetics, isotherms, and thermodynamic studies indicated that the adsorption of AMOX by AFC was an exothermic physic-adsorption. The interaction between AMOX and AFC surface was supposed to be a multiple-layer adsorption process for it is well fitted with the Freundlich model. The adsorption behavior could be described by pseudo-second-order model almost perfectly in kinetic studies. In addition, effect of pH, ionic strength, and reusability properties were also discussed in this paper. The AFC was proved to be the most rapid, efficient, and economically absorbent for AMOX removal, which was effective enough under various temperatures and saline circumstances. It was also proved useful, convenient, and renewable in dealing with the tough antibiotic pollutant problems and rebuilding of antibiotic sewage treatment facilities.
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JOURNAL OF FLUORESCENCE
·
2017
·Vol. 27
·期 5
·1721-1727
·
IF 1.461
Wang Fanwei,Duan Hongdong,Xing Dianxiang,Yang Geng
One novel turn-on fluorescence probe founded on conjugated pyrazole Schiff base for detecting Al 3+ was invented. The UV–vis and fluorescence spectrometer were employed to explore optical properties of this probe. The results got from those experiments indicated that this fluorescence probe manifested excellent sensitivity and selectivity for Al 3+ compared with other cations examined(Ag + , Co 2+ , Mg 2+ , Cu 2+ , Ni 2+ , Pb 2+ ,and Zn 2+ ). In addition, this probe displayed a more rapid response and remained stable between pH 6 and 9 by investigating the fluorescence intensity under different response time and various pH values. Remarkably, the detection limit for Al 3+ could lower to 1.0×10 −9 M. Therefore, the probe could be potentially applied to the environment for the detection of Al 3+ , and the availability in biological range of pH that could be further studied to make this probe apply to biological systems in the future.
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RESEARCH ON CHEMICAL INTERMEDIATES
·
2017
·Vol. 43
·期 8
·4719-4730
·
IF 1.369
Bao Lina,Wang Hong,Zhou Changqing,Cui Lu,Xin Baifu
The synthesis of high activity TiO 2 –CTAB–SiC composite possessing the microwave absorption capacity has been reported. Herein, the commercial Silicon carbide (SiC) particles were used as the microwave absorbent and supporter. TiO 2 nanopowders were loaded on the surface of SiC modified by hexadecyl trimethyl ammonium bromide (CTAB) via the hydrothermal route. The as-prepared TiO 2 –CTAB–SiC composite was characterized by X-ray diffraction, field-emission scanning electron microscopy, Fourier transformed infrared spectra, inductively coupled plasma-atomic emission spectrometry and fluorescence emission (PL). Its photocatalytic activity was evaluated by the degradation of rhodamine B in the microwave discharge electrodeless lamp (MDEL) system. The experimental results showed that TiO 2 –CTAB–SiC composite exhibited obviously higher photocatalytic activity in the MDEL system compared with pure TiO 2 nanopowders. Their reusability and separation performance were evaluated by recycling experiments. The reasons for the improvement in photocatalytic performance were discussed in detail.
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Natural Products and Bioprospecting
·
2016
·Vol. 6
·期 6
·297-303
·
IF 0
Geng Chang-An,Chen Ji-Jun
Swerilactones H–K ( 1 – 4 ) as four unprecedented secoiridoid trimers represent a new type of natural product, which has attracted much interest of natural chemists due to their novel skeletons and promising bioactivity. In order to well understand their MS fragmentation behaviors, they were investigated by electrospray ionization ion-trap time-of-flight multistage product ion mass spectrometry (ESI-IT-TOF-MS n ) for the first time. The protonated molecules ([M+H] + ) of swerilactones J and K, and deprotonated molecules ([M−H] − ) of swerilactones H, J and K were readily observed in the conventional single-stage mass spectra (MS); however only the [M+Cl] − ion for swerilactone I was obtained in negative mode. Based on the MS n study, the fragmentation pathways of swerilactones H and I in negative mode, and swerilactones J and K in both positive and negative modes were proposed. The neutral losses of H 2 O, CO, CO 2 and C 2 H 4 O moieties are the particular elimination from the precursor ions due to the presence of hydroxyl, δ -lactone and 1- O -ethyl moieties in their structures, of which the retro-Diels–Alder cleavage was the most particular dissociation. The fragment ions at m/z 341 and 291 in negative mode can be considered as the diagnostic ions for secoiridoid trimers. This investigation will provide valuable information for their fast characterization from complicated natural mixtures and extensive understanding their structural architectures.
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APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2016
·Vol. 182
·期 1
·55-66
·
IF 1.606
Fu Ruijie,Yao Kai,Zhang Qisheng,Jia Dongying,Zhao Jiayuan,Chi Yuanlong
A series of collagen hydrolysates (CHs) were prepared from pigskin shavings by using pepsin (PCH), trypsin (TCH), Alcalase (ACH), HCl (HCH), and NaOH (NCH). Their physicochemical properties, including degree of collagen hydrolysis, molecular weight distribution, electric charge, and microstructure, were investigated, and their flocculation performance was evaluated in a kaolin suspension, at varied pHs and concentrations. PCH exhibited high flocculation capability under acidic and neutral conditions, and its efficiency for removing suspended particles was approximately 80% at a concentration of 0.05 g/L. TCH, ACH, HCH, and NCH showed almost no flocculation capability. The flocculation capability of PCH could be mainly due to a combination of optimal molecular weight distribution and electric charge. This study could provide an environment-friendly natural flocculant and also proposes a promising approach for the reuse of collagen wastes.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2015
·Vol. 27
·期 4
·3514-3519
·
IF 1.569
Cao Renping,Fu Ting,Cao Yunling,Jiang Shenhua,Gou Qingdong,Chen Zhiquan,Liu Pan
Novel CaSb 2 O 6 :Eu 3+ , Bi 3+ phosphor is synthesized by solid-state reaction method in air. Their crystal structures are investigated. Luminescence properties of CaSb 2 O 6 :Eu 3+ , CaSb 2 O 6 :Bi 3+ , and CaSb 2 O 6 :Eu 3+ , Bi 3+ phosphors are compared, analyzed and explained, respectively. Tunable emission of CaSb 2 O 6 :Eu 3+ , Bi 3+ phosphor with excitation 298 nm can be observed by changing Eu 3+ or Bi 3+ concentration. Energy transfer (ET) and charge compensation in CaSb 2 O 6 :Eu 3+ , Bi 3+ phosphor are observed and analyzed via its luminescence properties. Lifetimes of CaSb 2 O 6 :5 %Eu 3+ , yBi 3+ phosphor (0.5 ≤ y ≤ 5 mol%) are investigated, which further confirm that ET process can be formed between Eu 3+ ion and Bi 3+ ion. The luminous mechanism is explained via the energy level diagrams of Bi 3+ and Eu 3+ ions. The emission properties indicate that CaSb 2 O 6 :Eu 3+ , Bi 3+ phosphor has a potential application for white LEDs based on near UV (~394 nm) LED chip.
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ARCHIVES OF PHARMACAL RESEARCH
·
2015
·Vol. 39
·期 2
·172-177
·
IF 2.046
Li Da-Hong,Li Chen-Xi,Jia Cui-Cui,Sun Ya-Ting,Xue Chun-Mei,Bai Jiao,Hua Hui-Ming,Liu Xiao-Qiu,Li Zhan-Lin
Three new xanthones, paucinervins H–J ( 1 – 3 ), as well as eleven known compounds ( 4 – 14 ), were isolated from the leaves of Garcinia paucinervis. The structures of the new compounds ( 1 – 3 ) were elucidated by 1D, 2D NMR spectra and HR ESIMS. In vitro antiproliferative activity against human promyelocytic leukemia HL-60 cells was tested, among which, compounds 2 , 5 , 6 and 7 exhibited strong growth inhibitory effects with GI 50 values ranging from 1.30 to 9.08 μM, respectively. Preliminary SARs were also discussed.
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JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY
·
2017
·Vol. 84
·期 1
·145-151
·
IF 1.575
Lin Xiuzhen,Guo Bing,Liu Peng,Fan Hongbo
Abstract Silver bromide (AgBr) rods with different polydispersity were first synthesized in situ in oil (dodecane)-in-water emulsion, where AgNO 3 was used as the silver ion source and tetradecyl trimethyl ammonium bromide acted as both the bromide ion source as well as the stabilizing agent. Scanning electron microscopy and X-ray diffraction techniques were carried out to characterize their morphologies and crystal structures. A morphology evolution varying with aging time showed that the AgBr rods in oil-in-water emulsion formed from nanoparticles, and the formation process was disclosed by drop-coating initially formed emulsion onto glass substrate and quick solvents evaporation. Besides, in comparison with commercial P25 (anatase 80%, rutile 20%), the as-fabricated AgBr rods presented excellent photocatalytic activities over methyl orange under direct sunlight irradiation, showing their promising potential as efficient sunlight-driven photocatalysts for organic pollutants degradation. Graphical abstract
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WATER AIR AND SOIL POLLUTION
·
2017
·Vol. 228
·期 8
·1-11
·
IF 1.702
Cheng Guanghuan,Sun Mingyang,Ge Xinlei,Ou Yang,Xu Xinhua,Lin Qi,Lou Liping
Black carbon (BC) is considered to be a promising novel material for controlling organic contaminants due to its strong adsorption property, low production cost, and less secondary pollution. However, seldom systemic research was conducted to investigate adsorption-desorption characteristics and interaction mechanism between BC and nonylphenol (NP), one kind of endocrine-disrupting contaminants (EDCs) and persistent organic pollutants (POPs). Therefore, in the present study, adsorption characteristics of NP on two BCs (rice straw black carbon (RC) and fly ash carbon (FC)) involving adsorption isotherm, kinetics, effect of pH, as well as desorption kinetics, were investigated to explore the feasibility of BC for remediation of NP pollution in a water environment. Adsorption isotherm data showed that Q
max was 61,889.21 ± 2777.68 and 6538.99 ± 606.72 mg/kg and n was 0.39 ± 0.037 and 0.55 ± 0.043 for RC and FC, respectively, suggesting the sorption capacity and nonlinearity of RC to NP is far higher than FC and indicating BC was an effective sorbent for NP pollution control, especially RC. The pH affected BC sorption capacity to NP by influencing the surface properties of BC and the NP speciation together. Desorption kinetics data indicated that more than 80% NP could not be released from both BCs, suggesting that BC could reduce NP releasing risk in a water environment evidently when BC is applied for NP pollution remediation.
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IONICS
·
2017
·Vol. 24
·期 2
·549-561
·
IF 2.062
Ma Guofu,Hua Fengting,Sun Kanjun,Feng Enke,Zhang Zhiguo,Peng Hui,Lei Ziqiang
The design of composited porous carbon-based electrode materials is keyed to improve the electrochemical performance for supercapacitors. Here, the natural carrot is used as a precursor to prepare porous carbon skeletons (CCs). Three different anthraquinones: anthraquinone (AQ), amino-anthraquinone (1-AAQ), and 2-aminoanthraquinone (2-AAQ) are used to modify the carbon skeletons with absorption. The maximum capacitance is obtained from material modified with 1-AAQ; it (1-AAQ-CC2) exhibits a noticeable pseudocapacitive performance with a brilliant capacitance of 328 F g −1 at 0.5 A g −1 and excellent cyclic stability (95% capacitance retention after 5000 cycles at 3 A g −1 ) in 2 mol L −1 KOH aqueous electrolyte. Moreover, the assembled 1-AAQ-CC2/1-AAQ-CC2 symmetric supercapacitor exhibits a high energy density of 14.8 Wh kg −1 at a power density of 240 W kg −1 operated at the voltage range of 0 to 1.8 V in 0.5 mol L −1 Na 2 SO 4 aqueous electrolyte. These results exhibit eco-friendly and low-cost design of organic and inorganic composite electrode materials for high-performance supercapacitors.
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