BIOSENSORS & BIOELECTRONICS
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2018
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IF 8.173
Shuli Lv,Jinliang Sheng,Shiyi Zhao,Mingchao Liu,Lihua Chen
It is a novel competitive challenge for electrochemical biosensor to directly, rapidly and ultrasensitively detect the disease markers in the whole serum due to biofouling caused by the complexity of actual samples. In this paper, poly (ethylene glycol) (PEG) and hyaluronic acid (HA) were utilized to modify Fe 3 O 4 @Au nanoparticles (NPs). Based on the successfully preparation and characterization of Fe 3 O 4 @ [email protected] @HA NPs with TEM , SEM, XRD, FTIR and EDS , respectively, a novel immunosensor of brucellosis with high selectivity , sensitivity and almost perfect protein-resistant properties in various external environments, especially, in complex biological systems was fabricated. More importantly, this immunosensor is capable of assaying brucellosis antibody in 100% serum without suffering from any significant biological interference. In addition, a wide linear response range from 10 –15 g mL −1 to 10 –11 g mL −1 towards antibody in 100% serum and a low limit of detection (LOD) of 0.36 fg mL −1 (3σ, n = 13) are demonstrated, which indicates that this immunosensor has a promising potential in clinical diagnosis.
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CERAMICS INTERNATIONAL
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2019
·
IF 3.45
Tong Chang,Yajun Hou,Fengze Wang,Yumei Wang,Yushui Bi
Semiconductor photocatalyst materials based on TiO 2 are potential photosensitizers for photodynamic therapy of cancer. Despite its effectiveness under ultraviolet light, pure TiO 2 shows almost no photocatalytic activity under visible light because of its wide intrinsic band gap energy (anatase, 3.2 eV). Besides, TiO 2 has no magnetic delivery property. In this study, an inorganic nanocomposite, N-TiO 2 /SiO 2 /Fe 3 O 4 , is successfully prepared in three steps by means of co-precipitation, Stöber and sol-gel methods. Deep characterization results by XRD , TEM, XPS , UV–vis DRS, FL and magnetic hysteresis loop reveal that N-TiO 2 /SiO 2 /Fe 3 O 4 has core-shell structure, magnetic property and visible-light-response capability. As a potential photosensitizer for photodynamic therapy, it shows a stronger photodynamic suppression effect on proliferation of HeLa cells than TiO 2 under the irradiation of xenon light.
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INTERNATIONAL JOURNAL OF PHARMACEUTICS
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2018
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IF 3.862
Ningxiang Yu,Taimei Cai,Yong Sun,Chengjia Jiang,Hua Xiong,Yanbin Li,Hailong Peng
In this study, a novel antibacterial agent was developed based on chitin nanofibrous microspheres loaded with AgNPs and Fe 3 O 4 nanoparticles (Ag-Fe 3 O 4 -NMs) for synergistic antibacterial activity and wound healing. Ag-Fe 3 O 4 -NMs was prepared via an in situ synthetic method which showed an excellent porosity and wettability. Moreover, Ag-Fe 3 O 4 -NMs were capable of sustained release of Ag + and catalysed the decomposition of low H 2 O 2 concentrations to generate hydroxyl radical ( OH). The OH and Ag + showed higher antibacterial activity and inhibited the toxicity with high dose of AgNPs and H 2 O 2 . In vitro biocompatibility results suggested that Ag-Fe 3 O 4 -NMs have low toxicity and low hemolysis. Thus, a novel antibacterial agent with enhanced synergistic antibacterial activity was obtained by combination of Ag-Fe 3 O 4 -NMs and H 2 O 2 at a low and biologlically safe dosage, which could facilitate fibroblast growth, accelerate epithelialization, and promote the healing rate of infected wounds.
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SENSORS AND ACTUATORS B-CHEMICAL
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2019
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IF 6.393
Xin Li,Bangxiang Liu,Kun Ye,Liang Ni,Xuechao Xu,Fengxian Qiu,Jianming Pan,Xiangheng Niu
Due to the role of phosphate (PO 4 3− ) as an indicator of eutrophication pollution, developing low-cost and high-performance approaches to detect it turns to be of great significance. Herein, we developed a highly sensitive and specific colorimetric sensor for PO 4 3− determination via utilizing Zr 4+ to synergistically inhibit the peroxidase-like activity of Fe 3 O 4 nanocubes. The decoration of hydrophilic 3,4-dihydroxyhydrocinnamic acid (DHCA) on Fe 3 O 4 endows it with enhanced ability to catalyze the 3,3′,5,5′-tetramethylbenzidine (TMB) chromogenic reaction. Because of electrostatic interaction, Zr 4+ is easy to be adsorbed onto the negatively charged Fe 3 O 4 -DHCA nanozyme. When PO 4 3− is added, it can interact with Zr 4+ and form a coating on Fe 3 O 4 -DHCA surface, significantly suppressing its peroxidase-mimicking activity. As a result, the TMB chromogenic reaction is seriously inhibited in the presence of PO 4 3− . Based on this strategy, PO 4 3− in the wide scope of 0.066–33.3 μM could be linearly determined, providing a detection limit down to 49.8 nM. Thanks to the strong and specific interaction between Zr 4+ and PO 4 3− , the colorimetric sensor could be employed to detect PO 4 3− with favorable selectivity. Reliable analysis of the target in practical water was also verified by our assay, indicating its great potential as a simple but efficient tool for environmental monitoring.
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BIOSENSORS & BIOELECTRONICS
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2012
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IF 5.602
Kun Shang,Jianying Zhu,Xiaomeng Meng,Ziqiang Cheng,Shiyun Ai
A novel electrochemical immunosensor for ultrasensitive detection of subgroup J of avian leukosis virus (ALVs-J) was designed by using graphene sheets (GS)-layered double hydroxides (LDHs) composites modified electrode with multifunctional Fe 3 O 4 core/Ni–Al LDHs shell (LDHs@Fe 3 O 4 ) nanospheres as labels. At first, the GS-LDHs were used for the immunosensor platform for improving the electronic transmission rate as well as increasing the surface area to capture a large amount of primary antibodies (Ab 1 ). After that, ferrocene (Fc), secondary antibodies (Ab 2 ) and horseradish peroxidase (HRP) multifunctional LDHs@Fe 3 O 4 nanospheres were used as labels with high load amount and good biological activity. Subsequently, in presence of H 2 O 2 , amplified signals were obtained by an electrochemical sandwich immunoassay protocol. To embody the signal amplification property of the protocol, the analytical properties of various immunosensor platform and labels were compared in detail. Under optimal conditions, the reduction peak currents of the electrochemical immunosensor were proportional to the ALVs-J concentration over the range from 10 2.32 to 10 5.50 TCID 50 /mL with a low detection limit (180 TCID 50 /mL, S/N=3). The resulting immunosensor also displayed a good selectivity, reproducibility and stability.
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MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 8
·1-11
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IF 5.3
Du Juan,Li Zongshuang,Liu Kai,Guo Jiangli,Bai Yanhong
A novel colorimetric aptasensor assay based on the excellent magnetic responsiveness and oxidase-like activity of Fe 3 O 4 @MIL-100(Fe) was developed. Fe 3 O 4 @MIL-100(Fe) absorbed with aptamer and blocked by BSA served as capture probe for selective isolation and enrichment of Listeria monocytogenes one of the most common and dangerous foodborne pathogenic bacteria. The aptamer absorbed on Fe 3 O 4 @MIL-100(Fe) was further used as signal probe that specifically binds with target bacteria conjugation of capture probe for colorimetric detection of Listeria monocytogenes , taking advantages of its oxidase-like activity. The linear range of the detection of Listeria monocytogenes was from 10 2 to 10 7 CFU mL −1 , with the limit of detection as low as 14 CFU mL −1 . The approach also showed good feasibility for detection of Listeria monocytogenes in milk and meat samples. The spiked recoveries were in the range 81–114% with relative standard deviations ranging from 1.28 to 5.19%. Thus, this work provides an efficient, convenient, and practical tool for selective isolation and colorimetric detection of Listeria monocytogenes in food. Graphical Abstract
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TALANTA
·
2025
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IF 6.1
Lijun Wang,Jiangchuan Tang,Shanshan Ren,Haixia Hu,Xiao Yang,Xianggui Chen
Although many colorimetric methods have been developed for on-site pesticide detection, most exhibit low sensitivity and limited resistance to interference. To address these issues, this study developed a novel dual-mode colorimetric-ratiometric fluorescence method for isocarbophos (ICP) detection using sulfur and nitrogen co-doped carbon nanodots (S, N-CDs) to inhibit catalytic Fe 3 O 4 @Cu-BTC nanozyme activity. The synthesized Fe 3 O 4 @Cu-BTC nanozymes with higher peroxidase-like activity were inhibited by S, N-CDs. The inhibitory mechanism was deeply investigated and was ascribed to (1) the restriction of the reaction between Fe 3 O 4 @Cu-BTC and hydrogen peroxide (H 2 O 2 ), which decreased the ·OH production, and (2) the ·OH scavenging ability of the S, N-CDs, forming the basis of the dual-mode method. The Fe 3 O 4 @Cu-BTC/Apt-complementary strand (CS)/S, N-CDs hybridization complexes demonstrated minimal to no peroxidase-like activity, preventing H 2 O 2 from oxidizing o -phenylenediamine (OPD) to produce yellow fluorescent 2,3-diaminophenazine (DAP). Meanwhile, the detection system demonstrated a low fluorescence intensity ratio (I 575 /I 465 ) at an excitation wavelength of 390 nm. However, the presence of ICP caused the CS/S, N-CDs to separate from the Fe 3 O 4 @Cu-BTC/Apt due to the high affinity between Apt and ICP. Then, the Fe 3 O 4 @Cu-BTC/Apt-ICP complexes recovered peroxidase-like activity, which promoted DAP production, accompanied by a pronounced fluorescence intensity ratio (I 575 /I 465 ). In optimum conditions, the detection limits (LODs) were 2.79 ng/mL (colorimetric) and 0.26 ng/mL (ratiometric fluorescence), respectively. The feasibility of the proposed method was verified via three spiked samples, yielding recovery rates of between 86.08 % and 112.64 %, indicating the significant potential for the highly sensitive, accurate on-site detection of ICP in actual samples.
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CHEMISTRY OF MATERIALS
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2015
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IF 8.354
Mingxia Jiao,Jianfeng Zeng,Lihong Jing,Chunyan Liu,Mingyuan Gao
PEGylated Fe3O4 nanoparticles were prepared through flow synthesis upon the pyrolysis of ferric acetylacetonate (Fe(acac)3) in anisole at 250 °C under pressure of 33 bar, in the presence of α,ω-dicarboxyl-terminated polyethylene glycol (HOOC–PEG–COOH) and oleylamine. In combination with theoretical analysis, the effects of linear velocity, residence time, and reactor dimension on particle size distribution were systematically investigated. In addition, the impact of Ostwald ripening on particle size distribution was also revealed. In particular, the impacts of monomer concentration distributions along both axial and radial directions of the tube reactor on the particle size distribution were carefully investigated. Under optimized conditions, PEGylated Fe3O4 nanoparticles with the relative standard deviation of particle size down to 10.6% were thus obtained. The resulting 4.6 nm particles exhibited excellent colloidal stability and high longitudinal relaxivity (r1) up to 11.1 mM–1·s–1, which manifested the reliability of flow synthesis in preparing PEGylated Fe3O4 nanoparticles as contrast agents for magnetic resonance imaging applications.
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TALANTA
·
2014
·
IF 3.511
Minqiong Tang,Qing Wang,Ming Jiang,Li Xu,Zhi-Guo Shi,Ting Zhang,Yan Liu
In the present study, magnetic nanoparticles (MNPs) with phenyl functionalized core and a hydrophilic methylcellulose coating were synthesized. The functionalized MNPs showed excellent dispersibility in aqueous solution and they were applied to magnetic solid phase extraction (MSPE) of sildenafil and its metabolite, desmethyl sildenafil, from human urine and plasma samples followed by high performance liquid chromatographic analysis. The factors that may influence the extraction, including the amount of MNPs, pH and salt concentration of sample solution, extraction and desorption time, and the volume of desorption solvent, were investigated in detail. Under the optimum MSPE conditions, the developed method showed satisfactory reproducibility with intra-day and inter-day relative standard deviations less than 8.2% and low limits of detection of 0.41–0.96 ng mL −1 from urine and plasma samples. The proposed material possessed good water compatibility and demonstrated excellent applicability for biological samples.
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RSC Advances
·
2014
·Vol. 4
·期 49
·25983-25992
·
IF 3.708
Jun Liu,Wenjing Wang,Huiwen Liu,Yaoliang Zhou,Haibo Zhang,Xiaohai Zhou
Herein, amino-epoxy supports were innovatively imported onto magnetic nanoparticles (Fe3O4–polymer hybrid nanospheres) for immobilizing enzymes. This new support has a coating layer with dual functional groups (epoxy and amino-epoxy). Consequently, this support has great anionic exchange power and a high number of epoxy groups. The acquired immobilized Penicillium expansum lipase in combination with this heterofunctional support represents a novel class of heterogeneous catalyst towards the synthesis of 1,3-dibutylurea from ethylene carbonate and butylamine, which has not been very commonly catalyzed by enzymes. After optimization of the reaction conditions, the yield of 1,3-dibutylurea was 77% under solvent free conditions at 60 °C. Moreover, after completion of reaction, the catalyst was simply recovered by an external conventional magnet and recycled without significant loss in the catalytic activity (up to ten cycles).
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RSC Advances
·
2015
·Vol. 5
·期 24
·18850-18857
·
IF 3.84
Huimin Duan,Leilei Li,Xiaojiao Wang,Yanhui Wang,Jianbo Li,Chuannan Luo
A chemiluminescence (CL) sensor with high sensitivity and selectivity has been developed for the determination of Ribonuclease A (RNase A). A new material Fe3O4/multi-walled carbon nanotubes/SiO2 (Fe3O4/MWCNTs/SiO2) was introduced into this sensor as a supporting material to prepare a surface molecular imprinting polymer (SMIP). In this work, Fe3O4 not only served as a backbone material in the preparation of the RNase A SMIP, but also as a separation reagent to allow the easy collection of the SMIP complex. Carbon nanotubes and SiO2 were also used as supporting materials to prepare SMIP for their large specific surface area. The Fe3O4/MWCNTs/SiO2 nanocomposite was characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) techniques. The adsorption ability of the Fe3O4/MWCNTs/SiO2-SMIP was calculated to be 102 mg g−1, which demonstrated the excellent recognition and adsorption ability of the imprinting cavities situated at or in the proximity of the surface of the Fe3O4/MWCNTs/SiO2. Under optimal conditions, the linear range of the sensor extended from 1.0 × 10−9 mg mL−1 to 1.0 × 10−7 mg mL−1 for RNase A and the detection limit was 3.2 × 10−10 mg mL−1 (3δ). The proposed sensor was successfully applied for the determination of RNase A in biological samples with recoveries from 93% to 105%.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2024
·
IF 9.4
Tian Tian,Xuefeng Hu,Yao Huang,Huixian Du,Chao Wang,Mina Yang
The widespread utilization of fossil fuels has resulted in a significant increase in CO 2 emissions, leading to a variety of environmental issues. The photocatalytic conversion of CO 2 into fuel presents an effective solution to both the energy crisis and environmental warming. Therefore, the selection of a suitable catalyst is paramount. However, traditional photocatalysts often encounter challenges such as rapid electron-hole recombination and limited exposure of active sites. To improve these limitations, this study introduces AFM-X, a heterojunction catalyst composed of Au/Fe 3 O 4 /MIL-101(Fe)-X, which facilitates the formation of open metal sites (OMS) that enhance the effective separation of photogenerated carriers. In AFM-X, OMS function as Lewis acid sites, thereby enhancing CO 2 adsorption. The presence of Au nanoparticles (NPs) introduces additional active sites for CO 2 reduction. The synergistic effect between OMS and Au NPs increases the catalyst’s active sites, while the heterojunction construction promotes electron transfer, thereby enhancing CO 2 photocatalytic reduction efficiency. The CO generation rate of AFM-2 reached 98.8 μmol g −1 h −1 , surpassing those of FM, MIL-101(Fe), Fe 3 O 4 , and Au NPs, by 4.1, 6.3, 6.2, and 3.2 times, respectively. Furthermore, AFM-2 maintains stable performance over a 40 h cycle test. In-situ DRIFTS spectroscopy reveals that CO 2 reduction occurs through two parallel pathways. This study offers new insights into the design of composite photocatalytic materials, highlighting the effectiveness of OMS Lewis acid sites in significantly enhancing the photocatalytic reduction of CO 2 .
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