CHEMISTRY & BIODIVERSITY
·
2025
·
IF 2.5
Li-li Luo,Jun-lei Chen,Cai-xia Du,Lie-jun Huang,Chun-mao Yuan,Jue Yang,Zhan-Xing Hu,Xiao-jiang Hao,Wei Gu
Three novel quinoline alkaloids, tetradiunitiside A ( 1 ), tetradiunitiside B ( 2 ), and glycohaplopine-6- O -α- l -rhamnopyranoside ( 3 ), along with eight known ones ( 4–11 ), were isolated from the fruits of Tetradium glabrifolium . Their structures were inferred by IR, 1D NMR and 2D NMR, and HR–ESI–MS spectra. All the isolated compounds were evaluated for their antibacterial activities. Compounds 1–11 had potential antibacterial activities against Bacillus gasoformans , Bacillus subtilis , Pseudomonas solanacearum , and Pseudomonas aeruginosa , with minimum inhibitory concentration (MIC) values ranging from 100 to 3.125 µg/mL. Among them, compound 6 showed the most significant antibacterial activity against four strains of bacteria. These findings elucidated the pharmacological substance basis for the antibacterial activity of T. glabrifolium to some extent. Graphical Three novel quinoline alkaloids together with eight known ones were isolated from the fruits of Tetradium glabrifolium . The antibacterial activity of compounds against four bacteria strains were screened, and 11 compounds exhibited significant antibacterial activity.
研究领域:
植物化学
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药理学
·
微生物学
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DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2018
·
IF 3.849
Yizhen Li,Yin Wan,Ruyi Li,Laojiu Xu,Mingyong Xie,Guiming Fu
In this study, the caffeoylquinic acid (CQA) extract solution of the Artemisia selengensis Turcz (AST) leaves prepared by 70% ethanol reflux method was separated with petroleum ether, ethyl acetate, and water-saturated n -butanol to obtain the proper solvent to purify CQAs. Results showed that the ethyl acetate fraction (EAF) exhibited the highest total chlorogenic acid content (TCC) (241.46 ± 0.00 mg/g dry fraction), followed by the water-saturated n -butanol fraction ( n BuF) (TCC = 62.30 ± 0.82 mg/g dry fraction). Ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry and high-performance liquid chromatography photodiode array were used to qualitatively and quantitatively determine the CQAs in these two fractions. In EAF, four CQAs, namely, 3-CQA, 3,5-diCQA, 3,4-diCQA, and 4,5-diCQA, were identified with the contents of 5.69 ± 0.03, 106.65 ± 0.83, 15.65 ± 0.24, and 28.08 ± 0.82 mg/g, respectively. Six CQAs, namely, 3-CQA, 4-CQA, 1,3-diCQA, 3,5-diCQA, 3,4-diCQA, and 4,5-diCQA, with contents of 16.57 ± 0.23, 3.22 ± 0.55, 4.49 ± 0.01, 16.86 ± 0.24, 7.35 ± 0.11, and 6.41 ± 0.13 mg/g, respectively, were identified in n BuF. Quantitative analysis of the six CQAs in AST leaves have not been reported, and 1,3-diCQA has been found in AST leaves for the first time. Moreover, either EAF or n BuF and the six CQA reference compounds showed certain inhibitory activity on xanthine oxidase (XOD) in vitro . EAF demonstrated a stronger XOD inhibitory effect (IC 50 = 1.67 mg/mL) than n BuF. Among the six CQA compounds, 3,4-diCQA had the lowest IC 50 of 12.80 μM, whereas 1,3-diCQA and 3-CQA had IC 50 values of 23.69 and 28.29 μM, respectively. The inhibitory activity of 4-CQA was weak. These findings indicated that CQAs are the major components inhibiting the XOD activity in vitro in AST leaves.
研究领域:
植物化学
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药理学
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产品关联
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2021
·
IF 7.312
Yunchang Fan,Hui Luo,Chunyan Zhu,Wenjin Li,Di Wu,Hongwei Wu
This work synthesized a series of hydrophobic natural alcohols based deep eutectic solvents (NADESs). These NADESs exhibited high extraction ability for quinine. The NADESs with lower viscosity and stronger hydrogen bond accepting ability ( β ) had stronger extraction ability. Quinine acted as hydrogen bond acceptor (HBA) for the hydrogen bonding between quinine and NADESs. The extraction efficiency of NADESs for quinine was higher than ionic liquid (IL), trichloromethane and ethyl acetate. After extraction, the NADES phase could be reused and quinine could be recovered from NADES phase via back extraction with 1.0% acetic acid aqueous solution as extraction solvent. The NADESs have the advantages of ease of preparation and high atom-economy and may be promising solvents for the extraction of bioactive compounds from natural sources compared with ionic liquids and conventional organic solvents.
研究领域:
化学
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药学
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产品关联
DOI
高影响因子
JOURNAL OF POLYMER RESEARCH
·
2021
·Vol. 28
·期 5
·1-10
·
IF 3.097
Lin Yali,Liu Yu,Li Siqi,Rui Lili,Ou Jiming,Wu Quanzhou,He Jianfeng
A kind of novel three-dimensionally ordered macroporous molecularly imprinted microspheres (3DMIMs) had been developed for selective recognition and separation of quinine based on the combination of colloidal crystal templating method and molecular imprinting technique. The 3DMIMs were derived from the spherical silica colloidal crystal involving a four-step templating process including infiltration of imprinted precursors, sealing of polyethylene glycol, initiation polymerization, as well as final removal of the colloidal crystal and imprinting template. The resulted 3DMIMs consisted of a three-dimensional, highly ordered and interconnected macroporous arrays with nanoscale thickness of polymer wall, in which numerous imprinted cavities were obtained. The kinetic and isothermal adsorption behavior of the 3DMIMs were investigated in detail. The results showed the 3DMIMs had a rapider mass transfer process, higher adsorption capacities and recognition specificity toward quinine compared with the traditional bulk imprinted polymers. In addition, the 3DMIMs exhibited obvious high permeability and low backpressure at high flow rate as column packing. Extraction experiments showed that the 3DMIMs could effectively separate quinine and its diastereoisomer, quinidine, directly from crude cinchona bark extract with a satisfactory selectivity and elution recovery.
研究领域:
材料科学
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药物化学
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产品关联
DOI
高影响因子
Nanoscale Research Letters
·
2014
·Vol. 9
·期 1
·1-10
·
IF 2.481
Li Chen,Wang Zhuan-Hua,Yu Deng-Guang,Williams Gareth R
This manuscript reports a new type of drug-loaded core-shell nanofibers that provide tunable biphasic release of quercetin. The nanofibers were fabricated using a modified coaxial electrospinning process, in which a polyvinyl chloride (PVC)-coated concentric spinneret was employed. Poly (vinyl pyrrolidone) (PVP) and ethyl cellulose (EC) were used as the polymer matrices to form the shell and core parts of the nanofibers, respectively. Scanning and transmission electron microscopy demonstrated that the nanofibers had linear morphologies and core-shell structures. The quercetin was found to be present in the nanofibers in the amorphous physical status, on the basis of X-ray diffraction results. In vitro release profiles showed that the PVP shell very rapidly freed its drug cargo into the solution, while the EC core provided the succedent sustained release. Variation of the drug loading permitted the release profiles to be tuned.
研究领域:
药剂学
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材料科学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2016
·
IF 3.138
Pan Hu,Jing Wang,Ruihua Huang
In this study, simultaneous removal of Cr(VI) and Amido Black 10B (AB10B) using quaternized chitosan coated bentonite was investigated by a batch method. The factors affecting adsorption process such as pH, adsorbent dosage, contact time and initial concentration and the interaction of two components in binary solutions have been studied. The results showed that the antagonism effect existed between Cr(VI) and AB10B in binary systems. The equilibrium time for Cr(VI) adsorption was less than the one for AB10B adsorption. The maximum adsorption capacities of the modified bentonite, according to the Langmuir isotherm model were 847.5 mg/g for AB10B and 66.6 mg/g for Cr(VI) at 298 K. The experimental results demonstrated that both Cr(VI) and AB10B were well described by the pseudo-second-order model. Thermodynamic study depicted that the adsorption of Cr(VI) and AB10B onto the modified bentonite was both spontaneous. The adsorption for Cr(VI) was exothermic while the one for AB10B was endoth- ermic in nature.
研究领域:
环境科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2019
·Vol. 9
·期 7
·3938-3945
·
IF 3.049
Jin-Rong Bai,Yan-Ping Wu,Grosu Elena,Kai Zhong,Hong Gao
The biofilm formation of Staphylococcus aureus on food contact surfaces is the main risk of food contamination. In the present study, we firstly investigated the inhibitory effect of quinic acid (QA) on biofilm formed by S. aureus. Crystal violet staining assay and microscopy analysis clearly showed that QA at sub-MIC concentrations was able to significantly reduce the biofilm biomass and cause a collapse on biofilm architecture. Meanwhile, fibrinogen binding assay showed that QA had obviously effect on the S. aureus bacteria adhesion. XTT reduction assay and confocal laser scanning microscopic images revealed that QA significantly decreased metabolic activity and viability of biofilm cells. In addition, qRT-PCR analysis explored the potential inhibitory mechanism of QA against biofilm formation, which indicated that QA significantly repressed the gene sarA and activated the gene agrA. Moreover, QA exhibited a highly ability to reduce the number of sessile S. aureus cells adhered on the stainless steel. So, it was suggested that QA could be used as a promising antibiofilm agent to control biofilm formation of S. aureus.
研究领域:
微生物学
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食品安全
·
分子生物学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Xuyuan Sun,Hao Liu,Zhengyuan Dai,Ying Wang,Li Li,Yaping Ding
A molecularly imprinted fluorescent aptasensor was designed for selective detection of quinine (Qn) based on dual functional monomers. In the sol-gel polymerization of molecularly imprinted polymers (MIPs), 3-aminopropyl triethoxysilane (APTES) and quinine aptamer (Apt) were employed as dual functional monomers, and Qn was the template molecule. Near-infrared carbon dots (RCDs) were used as fluorescence signal probe, and effectively avoided the interference of fluorescence emitted by Qn. Due to the diverse interaction among APTES, Apt and Qn, the near-infrared fluorescent molecularly imprinted aptasensor (RCDs-Apt@MIPs) exhibited higher specificity towards Qn than the sensor with APTES or Apt as a single functional monomer. The sensor had a wide linear range of 5.0–110.0 μM with a low detection limit of 1.7 μM. Satisfactory recovery between 93.4 % and 104.0 % with relative standard deviation from 1.30 % to 3.60 % in drinks, which showed great potential that this method can be used to detect Qn in food.
研究领域:
分析化学
·
食品科学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Yaxin Yang,Meifang Liao,Jing Ou,Yanyu Jiang,Lian Zhong,Jie Wen,Lujun Wang
The achievement of universal and efficient enantioseparation by chiral stationary phase (CSP) has been considered as challenging research. In this work, a bilayer-modified chiral stationary phase (named Sil-QN-VAN) based on vancomycin and quinine was constructed first. The chiral recognition abilities of quinine and vancomycin were combined on a single column, achieving the synergistic effect for enantioseparation. Compared with monochiral columns, the Sil-QN-VAN column exhibited excellent enantioseparation capability and could be used for separating multiple chiral drugs. The chiral separation mechanism was studied using molecular simulation techniques. In addition, the effects of temperature and injection volume on chromatographic separation were investigated. This work provides ideas for the development of efficient and universal chiral stationary phase materials.
研究领域:
分析化学
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药物化学
·
色谱技术
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Peihong Tong,Yongjuan Meng,Junyu Liang,Jianping Li
A molecularly imprinted electrochemiluminescence (MIP-ECL) sensor with high sensitivity and good structural stability was constructed using the new composite material ZIF-8-NH 2 as an imprinted membrane. The ZIF-8-NH 2 imprinted composite material was prepared through layer-by-layer self-assembly with carboxyl functionalized magnetic CoFe 2 O 4 nanoparticles as the carrier, ZIF-8-NH 2 precursor as the functional monomer, and cinchonine (CCN) as the template molecule. The ZIF-8 MIP@Au@CoFe 2 O 4 core–shell imprinted material was characterized using infrared spectrometry, scanning electron microscopy and X-ray diffraction, and the recognition performance of molecularly imprinted sensor was characterized through electrochemical impedance spectroscopy and electrochemiluminescence (ECL) method. When CCN was re-adsorbed by the imprinted membrane, the ECL intensities of the MIP sensor increased because CCN molecules can promote the ECL intensity of Ru(bpy) 3 2+ . As a result, the MIP sensor showed preeminent linear response to CCN in the range of 1.0 × 10 −12 – 3.1 × 10 −8 mol/L, with a detection limit of 7.0 × 10 −13 mol/L, which is much lower than that reported for other detection methods. The sensor shows good practicality and can be used to detect CCN in human serum with recoveries ranging from 96.0%–103.0%.
研究领域:
分析化学
·
材料科学
·
生物医学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2015
·
IF 3.391
Zhengyu Yan,Zhengwei Zhang,Yan Yu,Zhen Liu,Jianqiu Chen
A novel and highly sensitive flow-injection chemiluminescence method for the determination of potassium bromate (KBrO 3 ) has been developed. This method is based on the luminescence properties of the KBrO 3 –Na 2 SO 3 –quinine sulfate system in acid medium. Optimized experimental conditions and a possible mechanism were investigated. The relative chemiluminescence intensity responded linearly to the concentration of KBrO 3 in the range of 7.054 × 10 −6 –1.008 × 10 −4 mol/L with a detection limit of 2.116 × 10 −6 mol/L. The relative standard deviation (RSD) at 5.0 × 10 −5 mol/L KBrO 3 ( n = 12) was 2.3%. The proposed method was applied successfully to the determination of KBrO 3 in flour.
研究领域:
分析化学
·
食品安全检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ULTRASONICS SONOCHEMISTRY
·
2023
·
IF 8.4
Danli Wang,Yushi Wang,Zhenlei Zhang,Shaoping Qiu,Yawen Yuan,Gongshuai Song,Ling Li,Tinglan Yuan,Jinyan Gong
Dicaffeoylquinic acids (diCQAs) are found in a variety of edible and medicinal plants with various biological activities. An important issue is the low stability of diCQAs during extraction and food processing, resulting in the degradation and transformation. This work used 3,5-diCQA as a representative to study the influence of different parameters in ultrasonic treatment on the stability of diCQAs, including solvent, temperature, treatment time, ultrasonic power, duty cycle, and probe immersion depth. The generation of free radicals and its influence were investigated during the treatment. The stability of three diCQAs (3,5-diCQA, 4,5-diCQA and 3,4-diCQA) under the certain ultrasonic condition at different pH conditions was evaluated and found to decrease with the increase of pH, further weakened by ultrasonic treatment. Ultrasound was found to accelerate the degradation and isomerization of diCQAs. Different diCQAs showed different pattern of degradation and isomerization. The stability of diCQAs could be improved by adding epigallocatechin gallate and vitamin C.
研究领域:
食品科学
·
植物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）