TALANTA
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2026
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IF 6.7
Ning Sheng,Qinghui Zhao,Jianqiang Wang,Xu Yao,Boyuan Li,Xianyang Zeng,Jingwen Sun,Jingquan Sha
The development of high-performance oxidase-like nanozymes is crucial for advancing biocatalysis and biosensing. Polyoxometalates (POMs) are promising candidates but suffer from high solubility and inefficient interfacial electron transfer. Conventional immobilization often overlooks the matrix's role in catalysis. Herein, a dual-strategy enhanced nanozyme was developed by in-situ oxidative polymerization of a mono-V-substituted phosphomolybdic acid H 5 [PMo 11 VO 40 ] (PMo 11 V 1 ) into a conductive polypyrrole (PPy) matrix. The optimal composite PMo 11 V 1 @PPy-2 exhibits 2.2-fold higher oxidase-like activity than its conventional immobilized counterpart. Ultrasonic irradiation (100 kHz) further enhances its activity by ∼ 80% through improved mass transfer. Leveraging this synergy, a highly sensitive colorimetric biosensor for glutathione (GSH) detection was constructed, achieving a low detection limit (0.73 μM), a wide linear range (1-80 μM), excellent recovery in serum (99.4% to 100.6%), and high selectivity. This work demonstrates that rational integration of static material design with dynamic process engineering can overcome fundamental limitations in heterogeneous nanozyme catalysis, providing a generalizable strategy for developing high-performance enzymatic mimics for biomedical applications.
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高影响因子
JOURNAL OF BIOTECHNOLOGY
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2026
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IF 4
Yu Feng,Chun Yang,Huihua Ge,Ruifang Zhang,Yaxin Chen,Yanhong Zhou,Zicheng Wu,Zhou Deng,Hongchun Li,Guangya Zhang
Urate oxidase (Uox) has great potential in biotechnology. However, the exploration of Uox with superior stability and its simple preparation with high yield remains challenging. By fusing human ferritin with the Uox from the extremophile Deinococcus radiodurans , a novel 24-polymeric Uox (DrUox-F) with excellent properties was achieved. The DrUox-F with 98% purity was obtained through low-speed centrifugation with the yield of 890 mg/L, representing the highest yield currently reported. Remarkably, DrUox-F preserved 100% activity after 35 days at pH 10, which was significantly superior to DrUox. Furthermore, no significant activity loss was observed following 318 days of storage at 4 °C. The lyophilized DrUox-F power retained over 90% of original activity after 280 days of storage at room temperature. Notably, the half-life was extended approximately 40-fold (from 41 h to 1880 h) through the addition of mannitol as an exogenous stabilizer. Molecular docking results revealed that mannitol could specifically bind to multiple regions on DrUox-F, with hydrogen bonds as the dominant interactions. Thus, DrUox-F, with superior cost-effectiveness, high efficiency, and enhanced stability, provides a breakthrough solution for addressing the critical limitations of Uox.
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高影响因子
ANALYTICAL CHEMISTRY
·
2026
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IF 7.3
Qing Li,Wei Li,Liping Lu,Xiayan Wang
Cortisol is an important biomarker in response to psychological stress. Detecting its content in biological samples is crucial for gaining insights into individual stress and personalized healthcare. The limitations of traditional techniques for cortisol detection make them unsuitable for rapid and real-time analysis, thereby motivating the development of alternative sensing strategies. Herein, we report a molecularly imprinted polymer (MIP) electrochemical sensor based on laser-induced graphene (LIG), fabricated via a one-step electropolymerization that integrates a Cu2+-l-histidine metal complex as intrinsic redox probes and 3,4-ethylenedioxythiophene (EDOT) as a conductive scaffold. The morphology, structure, electrochemical performance, and adsorption kinetics of the MIP film were systematically characterized. This MIP/LIG has a wide linear range of 0.05 to 100 μM, covering its physiological concentration range, with a detection limit as low as 5.6 nM, which is far below the lowest cortisol level in human sweat. Furthermore, it shows good selectivity for structurally similar steroid hormones and common sweat metabolites. Its excellent reproducibility, renewability, and long-term stability make it suitable for the detection of cortisol in both artificial and real human sweat, and the results are consistent with the physiological circadian rhythm changes. Importantly, this work first proposes the redox-active MIP using metal complexes, providing a new strategy for the development of electrochemical sensors for nonelectroactive substances.
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高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2026
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IF 3.8
Xia Yong,Wang Qi,Wang Xu,Shan Liancong,Zhang Bo,Li Sheng,Guo Chang
The accurate and rapid detection of fluoroquinolone antibiotics (FQs) is crucial, yet their structural homology often leads to cross-reactivity that compromise accuracy. Herein, we designed a poly(3,4-ethylenedioxythiophene) doped with a poly(styrenesulfonate) (PEDOT:PSS)-based dual-channel organic electrochemical transistor (OECT) array on a flexible, low-cost polyimide substrate. By leveraging the synergistic catalysis of single-walled carbon nanotubes (SWCNTs) and gold nanoparticles (AuNPs), effective discrimination of three FQs was achieved using gate electrodes modified with only two Nafion/SWCNTs/AuNPs composites, differing solely in AuNP mass fraction—this significantly simplifies fabrication for an electronic tongue system (ETS). Aided by principal component analysis (PCA), the system discriminates and quantifies ofloxacin (OFL), levofloxacin (LEV), and ciprofloxacin (CIP) across a wide concentration range of 0.4–1000 μM, with limits of detection (LODs) of 0.32–0.40 μM, while exhibiting excellent selectivity, anti-interference, consistency, stability, and rapid response. The comprehensive performance of this platform establishes a practical foundation for portable FQ sensing in environmental and clinical applications. Graphical abstract
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高影响因子
DYES AND PIGMENTS
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2026
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IF 3.9
Shen Zhang,Zhuo Wang
In consideration of the potential threat, the establishment of efficient approach for fluazinam determination is necessary. Based on the superior property of carbon dots and biomass, sulfur doped biomass-based carbon dots (SBCDs) were constructed using a simple hydrothermal approach, in which peanuts were applied as carbon sources with the addition of methionine. The well-dispersed SBCDs displayed optimal emission wavelength of 435 nm under the excitation wavelength of 364 nm. Owing to the detection principle of fluorescence quenching through static quenching and inner filter effects, the SBCDs were used to detect fluazinam with a broad linear range of 0-100 μM and a detection limit of 0.026 μM. Additionally, the SBCDs also exhibited temperature sensing with a wide linear range of 15-95 °C. Moreover, the as-prepared SBCDs were effectively used for the determination of fluazinam in tap water and orange juice samples. The SBCDs displayed tremendous potential for the sensing of fluazinam and temperature.
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JOURNAL OF ALLOYS AND COMPOUNDS
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2026
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IF 6.7
Xiaoqian Liu,Linghui Hao,Caiqing Song,Qiaoling Shen,Meirou Jing,Jiaying Xu,Lijun Bian,Lijie Sun
Dopamine (DA) and uric acid (UA) are important biomarkers for various diseases, and the development of reliable detection platforms for their detection is of significant value. In this study, nickel manganese oxide (NMO) was synthesized via a hydrothermal method, followed by thermal treatment. The morphology, structure, and composition of the samples were systematically characterized using scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray photoelectron spectroscopy (XPS). To enhance the electrical conductivity of the material, NMO was composited with carboxylated carbon nanotubes (CCNT) to form an NMO/CCNT composite, which was then used for the simultaneous detection of DA and UA. Among the synthesized series of materials, the sample with a nickel-to-manganese molar ratio of 1:2 (denoted as N 1 M 2 O) exhibited the best sensing performance, which was attributed to its unique bimetallic synergistic effect. In the presence of ascorbic acid, the N 1 M 2 O/CCNT electrode showed a good linear response to both DA and UA within the concentration range of 1–15 μM. Furthermore, the sensor achieved satisfactory recovery rates of 96.5–105.2 % in fetal bovine serum, demonstrating its potential for practical application.
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2026
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IF 4.1
Liuyi Huang,Xinhui Gu,Fengna Xi
Establishing sensitive detection method for carbohydrate antigen 15–3 (CA15–3) is of great significance for the early diagnosis and therapeutic monitoring of breast cancer. Herein, an electrochemiluminescence (ECL) sensor was fabricated by growing Co 3 O 4 nanoparticles in-situ within nanochannels and employing a signal gating strategy, achieving highly sensitive detection of CA15–3. Using simple Stöber method, a silica nanochannel film (SNF) was deposited onto an indium tin oxide (ITO) glass electrode. This two-dimensional (2D) nanoporous silica film provided a confined space, within which Co 3 O 4 nanozymes were synthesized in situ. Co 3 O 4 served as a peroxidase (POD)-mimic nanozyme, enabling the catalytic conversion of hydrogen peroxide (H 2 O 2 ) into reactive oxygen species (ROS). Functioning as a nanocatalyst, Co 3 O 4 remarkedly enhanced the electrochemical oxidation of luminol, thereby boosting the ECL signal. This dual enhancement mechanism enabled the sensor to achieve high luminol-based ECL signal. The bio-recognition interface was constructed by functionalizing the outer surface of SNF with silane coupling agents and immobilizing aptamers. A measurable decrease in the ECL signal was generated due to the hindered diffusion of the emitter and co-reactant and increased interfacial resistance, which occurred when the aptamer-CA15–3 complex formed on the electrode surface. This response allowed for the quantification of CA15–3. Under optimal conditions, the developed aptasensor can detect CA15–3 in the range of 0.1 to 10,000 mU/mL, with a limit of detection of 26 μU/mL. Owing to its merits of simple fabrication, rapid analysis, high sensitivity, good reproducibility, and excellent selectivity, the developed immunosensor holds great potential for clinical diagnostics of tumor biomarkers.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2026
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IF 9.7
Ziyue Ju,Ruichan Lv,Ji Zhang,Delong Shi,Yitong Chen,Anees A. Ansari
Conventional techniques such as screen printing and hand-brushing, though scalable, often compromise on patterning resolution and material waste. In contrast, advanced inkjet printing offers a promising route for high-resolution and efficient manufacturing. In this study, a flexible electrode based on Ni-MOF material used high-precision scalable inkjet printing was developed for glucose sensing in human body fluids for wearable devices. Initially, Ni-MOF material with a high specific surface area and porous structure was synthesized via a hydrothermal method, and its potential for electrochemical detection was investigated. Experimental results demonstrated that the material exhibited high sensitivity and a broad detection range for glucose. Importantly, graphite and carbon nanotubes were incorporated to construct a Ni-MOF composite material with the composition ratio optimized to enhance conductivity and mechanical stability, for further application in flexible electrodes. Typically, a cost-effective, high-efficiency, and scalable fabrication process was achieved through high-precision inkjet printing technology, which enables 1200 dpi resolution while supporting ultra-large-scale printing up to 1200 × 1200 mm. Simutaneously, simulation-based optimization was conducted to determine the most effective electrode shape. As a result, the detection range of the inkjet-printed flexible electrode was extended to 0.005–2.63 mM, with an improved sensitivity of 12.2 μA·μM −1 ·cm −2 . Moreover, excellent mechanical stability and long-term durability were achieved, enabling precise differentiation of glucose fluctuations in low-concentration simulated sweat. Finally, a microfluidic channel to provide environment for the reaction was designed, thereby establishing a fundamental foundation for applying the flexible electrode in wearable devices.
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高影响因子
MATERIALS RESEARCH BULLETIN
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2026
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IF 5.7
Weilong Bao,Hang Lu,Fei Li,Siyuan Lu
Morphological and size control of Pt nanoparticles (PtNPs) has long been pursued to improve the catalytic performance of Pt-based electrochemical sensors during recent decades. In this study, a high‑performance hydrogen peroxide sensor was developed by electrochemically depositing PtNPs onto a polyaniline‑modified glassy carbon electrode (PANI/GCE). Subsequently, the PtNPs/PANI/GCE electrode was separately subjected to two distinct methods, namely square wave (SW) treatment and square wave voltammetry (SWV). It was found that SWV was able to alter the exposed surface of platinum nanoparticles from (111) to (200), which leads to the formation of the angular nano-edges on the original Pt nanospheres. This structural transformation effectively increased the number of active sites and enlarged the effective surface area for the catalytic redox reaction of hydrogen peroxide. Owing to such advantages, the prepared PtNPs/PANI/GCE-SWV electrode exhibits a wide linear range from 50 μM to 30 mM and an excellent sensitivity of 294.90 μA·mM −1 ·cm −2 for H 2 O 2 detection. In addition, SWV endowed the fabricated sensor with superior stability and reproducibility, thereby demonstrating promising prospects in the commercialization of stretchable and wearable biosensors.
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2026
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IF 4.1
Qing Yang,Jingsen Zhang,Mingyi Lv,Chuanjie Chen,Yue Hua,Hong Li,Lanbo Di
Regulating the cobalt valence state is a crucial strategy for enhancing nonenzymatic glucose detection. In this study, two self-supported Co 3 O 4 nanocomposites were fabricated on plasma-pretreated carbon cloth (CC P ) by electrodepositing an α-Co(OH) 2 precursor followed by either plasma treatment yielding Co 3 O 4 /CC P -P or calcination yielding Co 3 O 4 /CC P -C. Structural characterization reveals that plasma pretreatment enriches the CC P surface with abundant oxygen-containing functional groups (OCGs), significantly improving its hydrophilicity. In addition, the Co 3 O 4 /CC P -C exhibits a phase-pure structure enriched with multiple Co 3+ and surface-adsorbed oxygen (O Ads ). These properties coupled with its rapid electron-transfer capability significantly promote the glucose oxidation reaction. Compared with Co 3 O 4 /CC P -P, the Co 3 O 4 /CC P -C electrode presents markedly higher electrocatalytic activity toward glucose oxidation, featuring superior sensitivities of 6380 μA·mM −1 ·cm −2 (0.05–0.4 mM) and 2070 μA·mM −1 ·cm −2 (0.4–1.4 mM), along with a lower limit of detection (2.9 μM). The Co 3 O 4 /CC P -C electrode also exhibits excellent selectivity, good preparation repeatability (relative standard deviation (RSD) = 2.14%), operational repeatability (RSD = 1.36%) and favorable stability (RSD = 2.40%). This study provides a promising approach for developing efficient non-enzymatic glucose oxidation Co-based electrocatalysts, and gets insights into the influence mechanism by engineering the Co valence state.
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CHEMICAL ENGINEERING JOURNAL
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2026
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IF 12.5
He Zhao,Chu Xu,Caobin Zhou,Ling Zhang,Chunzhong Li
Existing triboelectric nanogenerators (TENGs) for wearable sweat sensors are plagued by low power density, which restricts their practical application. This study focuses on boosting TENGs output power via a fluorinated bi-layer electronic textile (e-textile) fabricated by electrospinning rotary collection, enabling self-powered dual functionality: real-time electrochemical sweat sensing and human motion detection. The key design includes a PCLU-HFDD negative triboelectric layer (molecularly incorporated with hexadecafluorodecanediol to enhance electron affinity) and a poly(lactic- co -glycolic acid) (PLGA) positive layer. Rotary collection creates vein-like nanostructures on PCLU-HFDD yarns (6.2 μm roughness, 114.8° water contact angle), increasing contact area. The PCLU-HFDD TENG achieves a high power density of 0.66 W m −2 , an open-circuit voltage (Voc) of 117.08 V, and excellent stability over 35,000 contact-separation cycles. Critically, integrated with both sweat-sensing and motion-detection capabilities, the e-textile enables 180-min continuous glucose monitoring (under sedentary conditions) and recognition of human motions. This work confirms that the power enhancement not only overcomes the bottleneck of TENGs for wearable sensors but also facilitates dual-modal health monitoring, advancing the development of practical wearable bioelectronics.
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MICROCHEMICAL JOURNAL
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2026
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IF 5.1
Jiaqi Geng,Yanxin Lv,Zhitan Huang,Faman Lin,Neha,Brij Mohan,Wei Sun
Chlorogenic acid (CGA), a dietary polyphenol with antioxidant and therapeutic benefits, can be harmful at high concentrations, necessitating sensitive detection in food. This study presents a ZrO 2 -ZrC-doped laser-induced graphene (ZrO 2 -ZrC-LIG) electrode prepared via a one-step laser carbonization of UiO-66/polyimide film. Structural analysis reveals a three-dimensional porous network with uniformly dispersed ZrO 2 and ZrC nanoparticles in graphene, which increases defect density, conductivity and surface area. The optimal conditions of laser-induced parameters such as power and carving depth for the electrode fabrication were selected. Under the optimal conditions, the electro-oxidation of CGA on the ZrO 2 -ZrC-LIG electrode was carefully investigated with electrochemical parameters calculated. The presence of ZrO 2 , ZrC and LIG exhibited an enhancement to the CGA oxidation with a pair of well-define redox peaks appeared, which was attributed to the faster electron transfer, higher electrocatalytic activity and synergistic effects. When integrated with a wireless portable electrochemical workstation, the fabricated sensor shows excellent analytical performance for CGA detection, including a wide linear range (0.1 μM to 1.0 mM), a low detection limit of 33 nM (3σ), along with high selectivity, stability, and reproducibility. When applied to apple and Eucommia ulmoides samples, it achieves the recoveries of 99.26–103.14%, confirming good analytical reliability. This simple and scalable method offers a promising strategy for developing flexible, portable electrochemical sensors for food safety and nutrition monitoring.
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