APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
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2023
·Vol. 107
·期 22
·6985-6998
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IF 5
Zhang Mengmei,Chen Yuan,Lai Jinghui,Wang Xingjie,Hu Kaidi,Li Jianlong,Li Qin,He Li,Chen Shujuan,Liu Aiping,Ao Xiaolin,Yang Yong,Liu Shuliang
Abstract The presence of cypermethrin in the environment and food poses a significant threat to human health. Lactic acid bacteria have shown promise as effective absorbents for xenobiotics and well behaved in wide range of applications. This study aimed to characterize the biosorption behavior of cypermethrin by Lactiplantibacillus plantarum RS60, focusing on cellular components, functional groups, kinetics, and isotherms. Results indicated that RS60 exopolysaccharides played a crucial role removing cypermethrin, with the cell wall and protoplast contributing 71.50% and 30.29% to the overall removal, respectively. Notably, peptidoglycans exhibited a high affinity for cypermethrin binding. The presence of various cellular surface groups including –OH, –NH, –CH 3 , –CH 2 , –CH, –P = O, and –CO was responsible for the efficient removal of pollutants. Additionally, the biosorption process demonstrated a good fit with pseudo-second-order and Langmuir–Freundlich isotherm. The biosorption of cypermethrin by L. plantarum RS60 involved complex chemical and physical interactions, as well as intraparticle diffusion and film diffusion. RS60 also effectively reduced cypermethrin residues in a fecal fermentation model, highlighting its potential in mitigating cypermethrin exposure in humans and animals. These findings provided valuable insights into the mechanisms underlying cypermethrin biosorption by lactic acid bacteria and supported the advancement of their application in environmental and health-related contexts. Key points
• Cypermethrin adsorption by L. plantarum was clarified.
• Cell wall and protoplast showed cypermethrin binding ability.
• L. plantarum can reduce cypermethrin in a fecal fermentation model.
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JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Xiaomeng Sun,Yue Zhao,Ling Liu,Yuxin Qiao,Chunjuan Yang,Xiaotong Wang,Qian Li,Yang Li
Environmental monitoring of pesticide residues in crops is essential for both food safety and environmental protection. Traditional methodologies face challenges due to the interference of endogenous compounds in peel and pulp tissues, often being invasive, labor-intensive, and inadequate for real-time observation of hazardous substance distribution. In this study, dynamic borohydride-reduced nanoparticles were employed as enhanced substrates. For the first time, surface-enhanced Raman spectroscopy (SERS) imaging was harnessed to enable whole-process visual detection of pesticide residues. The developed method is both stable and sensitive, boasting a detection lower limit below 1 pg/mL, coupled with robust quantitative analytical capabilities. This technique was successfully employed to detect residue signals across various crops and fruit juices. Furthermore, SERS imaging was utilized to map the distribution of pesticide residues from the exterior to the interior of fruits and vegetables. Vertex component analysis further refined the process by mitigating interference from plant autofluorescence. Collectively, this innovative strategy facilitates comprehensive pesticide residue monitoring, offering a potent tool for controlling hazardous substances in crops. Its potential applications extend beyond food safety, holding significant promise for sustainable agricultural production and enhanced environmental safeguarding.
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PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2023
·
IF 4.7
Cong Zhang,Bin Wan,Meng-Ru Jin,Xi Wang,Yu-Jing Wei,Ling Zhong,Bin Xia
Diaphorina citri Kuwayama is of great concern because of its ability to transmit devastating citrus greening illness (Huanglongbing). One strategy for controlling HLB may involve limiting the spread of D. citri . Insecticides using dsRNA target genes may be a useful option to control D. citri . The ecdysone receptor ( EcR ) and ultraspiracle ( USP ) are crucial for the growth and reproduction of insects. This study identified the genes for D. citri ecdysone receptor ( DcEcR ) and ultraspiracle ( DcUSP ). According to the qPCR data, DcUSP peaked at the 5th-instar nymph stage, while DcEcR peaked at the adult stage. Females expressed DcEcR and DcUSP at much higher levels than males. RNAi was used to examine DcEcR and DcUSP function. The findings demonstrated that inhibition of DcEcR and DcUSP delayed nymph development and decreased survival and eclosion rates. ds EcR caused adults to develop deformed wings, and ds USP caused nymphs to wither and die. Female adult ovaries developed slowly, and the females laid fewer eggs. Additionally, DcEcR and DcUSP were inhibited, increasing D. citri susceptibility to pesticides . These findings suggest that DcEcR and DcUSP are critical for D. citri development, growth, and reproduction and may serve as potential targets for D. citri management.
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CEREAL CHEMISTRY
·
2023
·Vol. 100
·期 5
·1106-1113
·
IF 2.4
Yuhang Wan,Xinyu Yuan,Jia Liu,Ding Zhang,Zhengyong Zhang,Min Sha
Background and Objectives Targeting the problems, like various residual pesticides, the high interference of sample matrix and the slow detection speed, this work studied the rapid screening method of multipesticide residues in rice by taking bensulfuron-methyl, propanil and cypermethrin as examples. Findings Rice matrix solution was obtained by using the improved QuEChERS method, matrix standard solution, and solvent standard solution were prepared to obtain the linear range and matrix effect of each pesticide. QuEChERS method could rapidly pretreat samples, and the matrix effect was soft, with matrix effect values lower than 16%. Mixed solutions of the three pesticides in four combinations were prepared within the linear range. The type of pesticides in rice was determined by support vector machine (SVM), and the pesticide concentration was predicted by partial least square regression (PLSR). SVM provided a supervised identification method for pesticide residues, making the recognition accuracy higher than 90%. The error of PLSR in predicting pesticide concentration was lower than 5.36%. Conclusions UV spectroscopy had fast test speed, so the combination of QuEChERS and UV spectroscopy could improve the efficiency of analysis. SVM combined with PLSR could preliminarily determine the type of pesticides in rice sample, and then accurately predict the concentration of pesticides. Significance and Novelty The QuEChERS sample pretreatment technology and UV spectroscopy in combination with chemometrics was expected to be a new technology for rapid detection of pesticide residues in samples.
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MACROMOLECULAR CHEMISTRY AND PHYSICS
·
2023
·Vol. 224
·期 15
·2300076
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IF 2.5
Yuhang Zhang,Lu Bai,Zhirui Xu,Siyuan Chen,Junping Zheng
Dynamic bonds are usually used to endow thermoset materials with excellent reprocessability. Nevertheless, this strategy sacrifices the thermal stability in most instances. In recent years, the dynamic silyl ether (Si O R) has attracted great attention due to its excellent thermal stability. However, the reprocessability of thermoset materials based on it is far from satisfactory because of the inactive dynamic exchange reaction. Herein, a species of activated silyl ether with neighboring groups is identified via small-molecule model reaction and the corresponding activation energy is calculated by reaction kinetics for the first time. Based on the activated silyl ether linkages, the poly(dimethylsiloxane) elastomers with high mechanical strength, effective reprocessability, and thermal stability are further designed and prepared. In a word, this work fills a research gap about the effect of neighboring groups on the dynamic characteristic of silyl ether and provides a theoretical basis for the construction of activated silyl ether linkages, endowing the materials with thermal stability and efficient reprocessability.
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ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Xuecheng Zhu,Hongfei Su,Huilin Liu,Baoguo Sun
The development of precise and efficient detection technologies to recognize λ-cyhalothrin (LC) in agricultural products has attracted attention worldwide due to its widespread use and notable toxic effects on humans. Herein, a novel fluorescence biomimetic nanosensor was elaborately designed based on Zn(II)-doped cyclo-ditryptophan (c-WW)-type peptide nanodots and incorporating molecularly imprinted polymer (c-WW/Zn-PNs@MIP) for LC assays. C-WW/Zn-PNs obtained by self-assembly with aromatic cyclic dipeptides as basic building blocks and coordination with Zn(II) have low-toxicity, photostability, and bright yellow fluorescence emission , as a sensitive signal transducer. High-affinity imprinting sites further endow c-WW/Zn-PNs@MIP with superior selectivity and reusability. Based on prominent merits, c-WW/Zn-PNs@MIP demonstrated a good linear range (1–360 μg/L) with a low limit of detection (LOD) (0.93 μg/L), fast kinetics in target capture (10 min), and strong practicability in the capture of LC from real samples (spiked recovery of 81.0–107.7%). Additionally, to attain onsite profiling of LC, a visual platform was developed by integrating c-WW/Zn-PNs@MIP with a smartphone-assisted optical device. This smart evaluation system can capture concentration-dependent fluorescent images and accurately digitize them, enabling quantitative analysis of LC. This study developed a fluorescent c-WW/Zn-PNs@MIP-based smart evaluation system as a novel platform for LC monitoring applications, which not only has enormous economic value but also great environmental health significance.
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JOURNAL OF HAZARDOUS MATERIALS
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2023
·
IF 13.6
Ming-Kang Jin,Qi Zhang,Yu-Tian Yang,Cai-Xia Zhao,Jian Li,Hongjie Li,Haifeng Qian,Dong Zhu,Yong-Guan Zhu
Worldwide, pyrethroids , such as cypermethrin, are the second most applied group of insecticides, however, their effects on the soil microbiome and non-target soil fauna remain largely unknown. Herein, we assessed the change of bacterial communities and antibiotic resistance genes (ARGs) of soil and in the gut of the model soil species Enchytraeus crypticus using a combination of 16S rRNA gene amplicon sequencing, and high-throughput qPCR of ARGs. Results indicate that cypermethrin exposure enriches potential pathogens (e.g. Bacillus anthracis ) in the soil and gut microbiome of E. crypticus , heavily disrupting the latter’s microbiome structure, and even disrupts activities of the E. crypticus immune system. The co-occurrence of potential pathogens (e.g. Acinetobacter baumannii ), ARGs, and mobile genetic elements (MGEs) revealed the increased risk of pathogenicity as well as antibiotic resistance in potential pathogens. Moreover, structural equation modeling demonstrated that the dissemination of ARGs was not only promoted by MGEs, but also by the ratio of the core to non-core bacterial abundance. Collectively, these results provide an in-depth view of the previously unappreciated environmental risk of cypermethrin on the dissemination of ARGs in the soil and non-target soil fauna.
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SENSORS AND ACTUATORS B-CHEMICAL
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2018
·
IF 5.667
Xiaozhou Li,Tianyue Yang,Youtao Song,Jinghai Zhu,Deli Wang,Wei Li
Here we describe a surface-enhanced Raman scattering (SERS)-based immunochromatographic assay (ICA) method for the dual detection of two pyrethroid pesticides cypermethrin and esfenvalerate. In the proposed ICA-SERS methods, antibody conjugated gold nanoparticles (AuNPs) were used as the SERS substrate while fluorescence tags labeled on AuNPs were used as the Raman reporters for SERS. Through the immuno-specific combination of antigen and antibody, Raman reporter labeled AuNPs were immobilized on the test line of the ICA strip and facilitated the SERS measurement. And by immobilizing two test lines designed for the detection of two pesticides, a simultaneous dual detection was accomplished. The LODs of the ICA-SERS system was 2.3 × 10 −4 and 2.6 × 10 -5 ng/mL for cypermethrin and esfenvalerate, respectively. The sensitivity was 3 to 4 times higher than ELISA and fluorescent-based ICA methods using the same indirect competitive immunoassay methods. For the three types of samples (tap water, river water, and milk), the recoveries ranged from 94.9% to 112%, with RSD values ranged from 1.3% to 13.5%. The research reveals that this ICA-SERS method possesses high specificity, sensitivity, and reproducibility, and can be reliably used to detect dual pesticides in real samples. An R Shiny-based application 2plex-speclysis was built and used for all the data analyses in this paper. The application was designed to incorporate all the analyses including spectral preprocessing, performance evaluation, and PCA analysis, and make the spectral analysis accessible for people without programming experience.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2019
·Vol. 411
·期 20
·5309-5316
·
IF 3.286
Zhang Dianwei,Tang Jiaqi,Liu Huilin
A highly selective and sensitive fluorescent probe for optosensing lambda-cyhalothrin (LC) was prepared. The probe was based on sulfur-doped carbon dots (CDs) coated with molecularly imprinted polymers (MIPs). Doping the CDs with sulfur and modifying the surfaces of the CDs with an ionic liquid enhanced the performance of the fluorescent probe. The selectivity of the probe was improved through the application of molecular imprinting technology utilizing acrylamide and 1-vinyl-3-butylimidazolium tetrafluoroborate [VBIm][BF 4 ] as functional monomers. The resulting probe was used to detect LC, which is a pesticide residue, in vegetables and tea. Under optimal detection conditions, the linear range of the probe was found to be 1–150 μg kg −1 and the limit of detection to be 0.5 μg kg −1 by analyzing excitation/emission maxima at 350/450 nm. The developed method was successfully used to determine LC in vegetables and tea, yielding recoveries of 98.90–116.93%. These results suggest that this fluorescent probe based on MIP-coated, room-temperature ionic-liquid-sensitized, sulfur-doped carbon dots has great potential to be utilized for the precise detection of LC in complex samples.
Graphical abstract
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TALANTA
·
2018
·
IF 4.244
Li’na Qiao,Sihua Qian,Yuhui Wang,Hengwei Lin
Pesticides play a critical role in improving crop yield in modern agriculture, but their residues significantly harm the environment and human health. Herein, a novel and simple colorimetric sensor array built on sulfuric acid assisted KMnO 4 fading strategy has been developed for pesticides detection and discrimination. This sensor array is facilely fabricated by KMnO 4 and sulfuric acid through simply adjusting their concentrations and ratios. Hierarchical clustering analysis (HCA) demonstrates that the as-fabricated colorimetric sensor array has a high dimensionality, and shows excellent capability to recognize common kinds of pesticides from potential interferants. Semi-quantitative detection was achieved through combining HCA and corresponding fitting curves. Moreover, the proposed sensor array was successfully applied to detect pesticide residues (e.g. carbaryl) in real samples. The strategy described herein will not only “maximally” simplify the design and fabrication approach, but expand the application fields of colorimetric sensor array methodology towards weak-reactive analytes.
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Analytical Methods
·
2017
·Vol. 9
·期 31
·4609-4615
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IF 1.9
Xuelian Wu,Xiaodong Lv,Jixiang Wang,Lin Sun,Yongsheng Yan
Based on the room-temperature phosphorescence (RTP) peculiarity of Mn-doped ZnS quantum dots (QDs), a bifenthrin sensor was fabricated by coupling molecular imprinted polymers (MIPs) on the surface of silane modified QDs via atom transfer radical polymerization (ATRP). The stepwise synthesis was realized with ZnS QDs-NH2-Br as the initiator, methacrylic acid (MAA) as the functional monomer, divinylbenzene (DVB) as the cross-linker, CuBr as the catalyst and 2,2′-bipyridine as the complexant. The smart RTP probe for bifenthrin was gained by surface molecular imprinting technology (SMIT) that can not only produce the stable RTP intensity of Mn-doped ZnS QDs, but can also enable more efficient recognition of the target molecule. The results presented show that the detection limit of the probe was 16.7 μg L−1. The RTP stability, selectivity and sensitivity of the bifenthrin sensor were evaluated by using a spectrophotometer. The high sensitivity was due to an electron-transfer mechanism between the target and the QDs, and the particular selectivity was due to SMIT. Therefore, this novel strategy combines RTP of Mn-doped ZnS QDs with ATRP to use the best aspects of both. It offers the advantages of straightforward preparation and processing, fast response, low toxicity and optical stability, so it is very promising for the detection of bifenthrin.
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JOURNAL OF CHROMATOGRAPHY A
·
2025
·
IF 4
Sitian Gu,Wenli Zhu,Yufei Liu,Huixiao Duo,Qingli Yang,Xiudan Hou
Efficient enrichment of analytes and purification of matrices are crucial for the highly sensitive detection and monitoring of pesticides in traditional Chinese herbs. This work prepared magnetic ionic liquid-controlled covalent organic framework (IL-COF@Fe 3 O 4 ) as the sorbent via a simple in-situ precipitation polymerization and thiolene “click” strategy. The IL-COF@Fe 3 O 4 exhibited remarkable adsorption performance towards pyrethroids within 5 min. The adsorption of four pyrethroids on the surface of IL-COF@Fe 3 O 4 was according with Langmuir model and pseudo-second-order kinetic model. The adsorption energies were theoretically calculated, which were permethrin>cypermethrin>fenvalerate>bifenthrin. The modification of ILs improved extraction capacity mainly because of the interaction of imidazole and Cl or F and the pore size effect. This method was developed for the rapid extraction of four pyrethroids in Codonopsis pilosula and Angelica sinensis . The linear range was 0.05–200 μg L -1 . Matrix effects were ranging from -16.14% to 9.53%, indicating the strong matrix anti-interference ability of IL-COF@Fe 3 O 4 .
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