PLANT AND CELL PHYSIOLOGY
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2018
·Vol. 59
·期 9
·1889-1904
·
IF 4.059
Zhang Wei,Lu Li-Ying,Hu Li-Yan,Cao Wei,Sun Kai,Sun Qi-Biao,Siddikee Ashaduzzaman,Shi Run-Han,Dai Chuan-Chao
Allelopathy is mediated by plant-derived secondary metabolites (allelochemicals) which are released by donor plants and affect the growth and development of receptor plants. The plant root is the first organ which senses soil allelochemicals this results in the production of a shorter primary root. However, the mechanisms underlying this process remain elusive. Here, we report that a model allelochemical benzoic acid (BA) inhibited primary root elongation of Arabidopsis seedlings by reducing the sizes of both the meristem and elongation zones, and that auxin signaling affected this process. An increase in auxin level in the root tips was associated with increased expression of auxin biosynthesis genes and auxin polar transporter AUX1 and PIN2 genes under BA stress. Mutant analyses demonstrated that AUX1 and PIN2 rather than PIN1 were required for the inhibition of primary root elongation during BA exposure. Furthermore, BA stimulated ethylene evolution, whereas blocking BA-induced ethylene signaling with an ethylene biosynthesis inhibitor (Co2+), an ethylene perception antagonist (1-methylcyclopropene) or ethylene signaling mutant lines etr1-3 and ein3eil1 compromised BA-mediated inhibition of root elongation and up-regulation of auxin biosynthesis-related genes together with AUX1 and PIN2, indicating that ethylene signal was involved in auxin-mediated inhibition of primary root elongation during BA stress. Further analysis revealed that the BA-induced reactive oxygen species (ROS) burst contributed to BA-mediated root growth inhibition without affecting auxin and ethylene signals. Taken together, our results reveal that the allelochemical BA inhibits root elongation by increasing auxin accumulation via stimulation of auxin biosynthesis and AUX1/PIN2-mediated auxin transport via stimulation of ethylene production and an auxin/ethylene-independent ROS burst.
研究领域:
植物学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
Foods
·
2021
·Vol. 10
·期 12
·2976
·
IF 4.35
Jianying Zhao,Jing Qian,Hong Zhuang,Ji Luo,Mingming Huang,Wenjing Yan,Jianhao Zhang
This study aimed to investigate effects of plasma-activated solution (PAS) on the cell biology ofStaphylococcus aureusand qualities of fresh lettuce leaves. PAS was prepared by dielectric barrier discharge plasma and incubated withS. aureusfor 10–30 min or with lettuces for 10 min. Effects on cell biology were evaluated with microscopic images, cell integrity, and chemical modification of cellular components. Effects on lettuce quality were estimated with the viable microbial counts, color, contents of vitamin C and chlorophyll, and surface integrity. PAS reducedS. aureuspopulation by 4.95-log and resulted in increased cell membrane leakage. It also resulted in increased contents of reactive oxygen species in cells, C=O bonds in peptidoglycan, and 8-hydroxydeoxyguanosine content in cellular DNA, and reduced ratios of unsaturated/saturated fatty acids in the cell membrane. PAS treatment reduced bacterial load on fresh lettuce and had no negative effects on the quality. Data suggest that PAS can be used for the disinfection of ready-to-eat fresh vegetables.Keywords:reactive oxygen species;peptidoglycan;cell membrane;DNA;lettuces
研究领域:
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2018
·
IF 4.946
Taotao Li,Dingding Shi,Qixian Wu,Zhengke Zhang,Hongxia Qu,Yueming Jiang
The effect of sodium para -aminosalicylate (PAS-Na) on litchi pericarp browning and the potential regulating mechanism was investigated in this study. Results showed that 0.3 g L −1 PAS-Na significantly inhibited the development of pericarp browning and reduced respiration rate of litchi fruit. PAS-Na inhibited the production of reactive oxygen species (ROS) and decreased the expression level of senescence-related genes. Additionally, PAS-Na treatment enhanced the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX), which might contribute to the scavenging of ROS. Meanwhile, PAS-Na treatment maintained membrane integrity as indicated by reduced relative membrane leakage rate and malondialdehyde (MDA) content, as well as lower activities of membrane lipids-degrading enzymes: lipase and lipoxygenase (LOX). Amino acids, especially GABA, Glu, Met contents were also significantly affected by PAS-Na treatment. Taken together, we postulated that PAS-Na treatment might be a promising method for controlling postharvest browning and prolonging shelf-life of harvested litchi fruit.
研究领域:
植物生理学
·
食品科学
✓ 已验证
DOI
PubMed
高影响因子
TREES-STRUCTURE AND FUNCTION
·
2021
·Vol. 36
·期 2
·711-721
·
IF 2.529
Xu Chenyi,Ma Yuandan,Tian Zhengfeng,Luo Qingyun,Zheng Tiefeng,Wang Bin,Zuo Zhaojiang
Key message The four chemotypes of adult C. camphora released different monoterpenes which protected these plants against high temperature by lowering ROS levels and maintaining photosynthetic abilities. Abstract High temperature seriously impacts plant growth and development, and terpenes are believed to improve plant thermotolerance through indoor experiments using seedlings. To uncover the actual protection effects of monoterpenes on outdoor adult plants, we investigated the monoterpene emissions, reactive oxygen species (ROS) metabolism, and photosynthetic abilities in four chemotypes of adult Cinnamomum camphora that were blocked monoterpene synthesis by fosmidomycin (Fos) under high temperature in summer (the mean highest temperature at about 38.2 °C). Eucalyptol (EuL), linalool (Lnl), borneol (BeL), and camphor (CmR) chemotypes of C. camphora released an abundance of monoterpenes, with eucalyptol, linalool, endo-borneol, and camphor as the typical monoterpene, respectively. When the four chemotypes of C. camphora were treated with Fos, their monoterpene emission was inhibited remarkably, with disappearance of some monoterpenes. In that case, the content of O 2 − , H 2 O 2 , and thiobarbituric acid reactive substance (TBARS) in the four chemotypes of C. camphora significantly increased although antioxidant enzyme activities remarkably increased, and the photosynthetic abilities significantly decreased by declining the quantum production and electron transport in photosystem II and increasing the dissipation of absorbed light energy. Among the four chemotypes, monoterpenes might play the maximum roles in CmR lowering ROS levels and in EuL maintaining photosynthesis. It can be speculated that monoterpenes can protect adult C. camphora against high temperature by lowering ROS levels and maintaining photosynthetic abilities.
研究领域:
植物生理学
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2024
·
IF 10.6
Jinfeng Shi,Jiahui Zhou,Bo Liu,Kezhou Lin,Xingliang Xie,Xue Han,Yanmei Sheng,Yihan Liu,Congjian He,Yujin Zhou,Nan Zhu,Qian Yang,Ruifeng Luo,Yi Li
Background The oral administration of drugs for treating ulcerative colitis (UC) is hindered by several factors, including inadequate gastrointestinal stability, insufficient accumulation in colonic lesions, and uncontrolled drug release. Methods A multiple sensitive nano-delivery system comprising β-cyclodextrin (CD) and 4-(hydroxymethyl)phenylboronic acid (PAPE) with enzyme/reactive oxygen species (ROS) sensitivity was developed to load celastrol (Cel) as a comprehensive treatment for UC. Results Owing to the positive charge in the site of inflamed colonic mucosa, the negatively charged nanomedicine (Cel/NPs) could efficiently accumulate. Expectedly, Cel/NPs showed excellent localization ability to colon in vitro and in vivo tests. The elevated concentration of ROS and intestinal enzymes in the colon microenvironment quickly break the CD, resulting in Cel release partially to rebalance microbiota and recover the intestinal barrier. The accompanying cellular internalization of residual Cel/NPs, along with the high concentration of cellular ROS to trigger Cel burst release, could decrease the expression of inflammatory cytokines, inhibit colonic cell apoptosis, promote the macrophage polarization, scavenge ROS, and regulate the TLR4/NF-κB signaling pathway, which certified that Cel/NPs possessed a notably anti-UC therapy outcome. Conclusions We provide a promising strategy for addressing UC symptoms via an enzyme/ROS-sensitive oral platform capable of releasing drugs on demand. Graphical Abstract Supplementary Information The online version contains supplementary material available at 10.1186/s12951-024-02725-9.
研究领域:
药学
·
纳米技术
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胃肠病学
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炎症研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nano-Micro Letters
·
2020
·Vol. 12
·期 1
·1-18
·
IF 12.264
Li Yang,Lin Jinyan,Wang Peiyuan,Luo Qiang,Zhu Fukai,Zhang Yun,Hou Zhenqing,Liu Xiaolong,Liu Jingfeng
A pH/ROS cascade-responsive carrier-free nanodrug with self-targeting activation and ROS regeneration abilities.
On-demand release of VES in response to intracellular ROS to accelerate drug release via positive-feedback loop.
Highly synergistic therapeutic efficiency via orchestrated cooperation of oxidation-chemotherapy.
研究领域:
癌症研究
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纳米医学
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药剂学
·
诊疗学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Yinghua Guo,Xinru Wang,Yan Feng,Xiao Luo,Xiangming Meng,Xuhong Qian,Youjun Yang
Autophagy is a physiological process governing proteostasis and implicated in many diseases. Enhanced ROS levels was known to be associated with lysosomal autophagy and therefore a lyso-specific ROS probe could find applications in autophagy related studies. Following the probe-design principle of bridging-group modification, we previously developed a ROS probe capable of detect a broader range of reactive species than the existing probes. Additional merits of the probe include ratiometry-capability, minimal probe-product spectral crosstalk, resistance toward autoxidation at lysosomal acidity, and structural simplicity. It is the aim of this manuscript to render this probe lyso-specificity and examine its potentials to monitor autophagy-related ROS generation in HeLa cells treated by H 2 O 2 or 6-OHDA.
研究领域:
细胞生物学
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生物化学
·
医学研究
✓ 已验证
DOI
高影响因子
Rice Science
·
2025
·
IF 6.1
Chen Ya,Liu Zhiquan,Yang Linyin,Wu Fujie,Cao Zijian,Shi Huanbin,Qiu Jiehua,Kou Yanjun
The cell surface receptor chitin elicitor receptor kinase 1 (CERK1) is a well-known component of plant immunity. In a previous study, we found that OsCERK1 is also involved in regulating copper (Cu) uptake in rice, though the underlying mechanisms remain unclear. In this study, we identified proteins that interact with OsCERK1 and discovered a novel heavy metal-associated (HMA) domain-containing protein, OsHPP08. Our findings show that OsCERK1 phosphorylates and stabilizes OsHPP08. Structural analysis using AlphaFold, a yeast sensitivity assay of the Cu uptake-deficient yeast mutant, and Cu level measurements in the oshpp08 mutants and overexpression plants ( OsHPP08OE ) revealed that OsHPP08 facilitates Cu uptake. Moreover, rice seedling infection assays demonstrated that OsHPP08 positively contributes to blast resistance, and both OsCERK1 and OsHPP08 are necessary for Cu-modulated blast resistance. Further analyses suggest that OsCERK1 and OsHPP08 likely enhance blast resistance by regulating the antioxidant system and increasing the accumulation of H 2 O 2 . In conclusion, OsCERK1 promotes Cu uptake by stabilizing OsHPP08, and together they contribute to Cu-modulated blast resistance likely by modulating ROS accumulation. These results deepen our understanding of the interplay between biotic and abiotic signals in rice.
研究领域:
植物学
·
分子生物学
·
农业科学
✓ 已验证
产品关联
DOI
高影响因子
Toxics
·
2025
·Vol. 13
·期 5
·406
·
IF 4.1
Huabin Liu,Chuangyang Bai,Jiahui Cai,Yue Wu,Changwei Zhu
The phytotoxicity of aluminum (Al) to plants is well known. Auxin accumulation and reactive oxygen species (ROS) burst induced by Al toxicity are the key factors in root growth inhibition. Yucasin, an auxin synthesis inhibitor, effectively ameliorates Al phytotoxicity in tomato seedlings. However, the physiological mechanisms by which yucasin alleviates Al phytotoxicity in tomatoes remain elusive. Here, we examined the regulatory mechanisms of yucasin involved in tomato seedling growth under Al conditions through phenotypic, plant physiology analysis, and cellular experiments. Exogenous indole-3-acetic acid (IAA) application increased Al accumulation in tomato seedling roots, while yucasin decreased Al accumulation. Yucasin application reduced Al-induced ROS accumulation, lipid peroxidation, and cell death, enhanced root viability, and promoted tomato seedling root growth. Further, yucasin enhanced the antioxidant enzyme activities of superoxide dismutase, catalase, and peroxidase in plants under Al conditions. The results suggest that yucasin improves the scavenging capacity of ROS by maintaining the activities of antioxidative enzymes. This study elucidates the physiological mechanism by which yucasin alleviates Al phytotoxicity, highlighting its potential to enhance plant tolerance under acidic Al conditions.
研究领域:
植物学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF POLYMER SCIENCE
·
2024
·
IF 3.9
Xing Wang,Haohao Cui,Qingyun Ning,Huayang Feng,Xue Sun,Boyuan An,Zhanrong Li,Jianliang Shen,Jingguo Li
The excessive accumulation of reactive oxygen species (ROS) on wound surfaces poses considerable challenges to the wound healing process, as it exacerbates inflammation and heightens the risk of skin infections. Consequently, the development of multifunctional dressings that possess both ROS-scavenging and antimicrobial properties is of clinical significance for enhancing wound healing. To address these challenges, we synthesized a dual-scavenging hydrogel utilizing dual-dynamic crosslinking techniques. This hydrogel is composed of poly-lysine-grafted D-glucono-1,5-lactone, dithiodipropionic acid/lipoic acid, and dopamine (PLGDTD), in conjunction with borax. The multifunctional attributes of the hydrogel can be attributed to the incorporation of borate esters, dopamine, and disulfide bonds. Experimental results demonstrate that this hydrogel exhibits a robust antioxidant capacity and a pronounced inhibitory effect against Staphylococcus aureus and Escherichia coli . The results of cellular studies indicate that the hydrogel is capable of effectively scavenging ROS and mitigating oxidative stress, attributable to its dual-scavenging mechanism involving borate and disulfide bonds. This multifunctional hydrogel demonstrates significant potential as a candidate for enhancing wound healing associated with ROS. Graphical
研究领域:
生物材料学
·
医学工程
·
药剂学
✓ 已验证
产品关联
DOI
高影响因子
PLANT PHYSIOLOGY
·
2024
·
IF 6.5
Chen Kai,Guo Dongdong,Yan Jiji,Zhang Huijuan,He Zhang,Wang Chunxiao,Tang Wensi,Chen Jun,Xu Zhaoshi,Ma Youzhi,Chen Ming
Soybean (Glycine max [L.] Merr.) is a valuable oil crop but is also highly susceptible to environmental stress. Thus, developing approaches to enhance soybean stress resistance is vital to soybean yield improvement. In previous studies, transcription factor Alfin has been shown to serve as an epigenetic regulator of plant growth and development. However, no studies on Alfin have yet been reported in soybean. In this study, the endoplasmic reticulum (ER) stress- and reactive oxygen species (ROS)-related GmAlfin09 was identified. Screening of genes co-expressed with GmAlfin09 unexpectedly led to the identification of soybean peroxidase 6 (GmPRDX6). Further analyses revealed that both GmAlfin09 and GmPRDX6 were responsive to ER stress, with GmPRDX6 localizing to the ER under stress. Promoter binding experiments confirmed the ability of GmAlfin09 to bind to the GmPRDX6 promoter directly. When GmAlfin09 and GmPRDX6 were overexpressed in soybean, enhanced ER stress resistance and decreased ROS levels were observed. Together, these findings suggest that GmAlfin09 promotes the upregulation of GmPRDX6, and GmPRDX6 subsequently localizes to the ER, reduces ROS levels, promotes ER homeostasis, and ensures the normal growth of soybean even under ER stress. This study highlights a vital target gene for future molecular breeding of stress-resistant soybean lines.
研究领域:
植物生物学
·
分子遗传学
·
农业科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Guopei Zhang,Cong Wang,Xiaoyang Zhang,Yong Dong,Zhaomin Li,Leteng Lin
Effective elemental mercury (Hg 0 ) removal from syngas is essential for industrial mercury control. This study synthesized LaMeO x /ZSM-5 (Me = Zn, Cu, and Fe) sorbents via the sol–gel method and evaluated their Hg⁰ removal performance for simulated syngas at 50–250 °C. The physico-chemical properties of samples were characterized by Brunauer-Emmett-Teller (BET), Scanning Electron Microscopy (SEM), X-ray diffraction (XRD), H 2 temperature-programmed reduction (H 2 -TPR) and X-ray photoelectron spectroscopy (XPS). La 2 CuO 4 /ZSM-5 demonstrated superior efficiency (≥95 %) at 50–200 °C under simulated syngas, attributed to its porous structure and abundant surface-active oxygen. H 2 S significantly enhanced Hg 0 removal by forming active sulfur species, while H 2 , CO, and H 2 O inhibited performance due to competition for active sites. The inhibitory effects were mitigated by adding 500 ppm H 2 S. XPS and TPD analyses revealed HgS (cinnabar) as the dominant mercury species, with Cu 2+ ions and active oxygen critical for Hg 0 capture. Stability tests confirmed excellent reusability, with over 88 % efficiency maintained after 10 adsorption-regeneration cycles. La 2 CuO 4 /ZSM-5 emerges as a promising sorbent for Hg 0 removal, offering high efficiency, stability, and resistance to syngas components, making it suitable for industrial applications.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）