Microorganisms
·
2026
·Vol. 14
·期 2
·327
·
IF 4.2
Ben Xu,Xiran Chen,Jinmei Chai,Yunlin Wei
Previous studies have demonstrated that catecholamines, including epinephrine (Epi), norepinephrine (NE), and dopamine (DA), function both as xenosiderophores for bacteria possessing dedicated transport channels and as potential quorum-sensing signaling molecules or regulatory factors. However, current research on the interactions between dopamine and bacteria remains relatively limited. In this study, treatment ofEscherichia coli(E. coli) ATCC 11303 with a specific concentration of dopamine resulted in a 33.63% increase in the maximum growth biomass, a 47.32% enhancement in biofilm formation, a 24.60% increase in protease activity, a 68.81% improvement in swimming motility, and increases of 33.77% and 47.67% in chemotaxis and swarming motility, respectively. Transcriptome analysis revealed that dopamine promoted the expression of numerous iron uptake-related genes, while biofilm formation-related genes and virulence genes were concomitantly upregulated. High-performance liquid chromatography–mass spectrometry (HPLC-MS) and isotope ratio mass spectrometry (IRMS) analyses confirmed thatE. coliATCC 11303 can uptake dopamine, suggesting the existence of specific transport pathways. Multi-omics analysis revealed significant regulatory effects on metal ion transport, amino acid metabolism, purine metabolism, environmental adaptation, quorum sensing, two-component systems, and xylene degradation pathways. Dopamine may act as both a xenosiderophore and a signaling molecule, thereby modulating multiple critical physiological and biochemical processes and promoting bacterial growth. These findings provide valuable insights into the development of novel exogenous xenosiderophores and signaling modulators, advancing our understanding of microbial interactions with their host environment and contributing to the field of microbial endocrinology.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Cell Host & Microbe
·
2026
·
IF 23.2
Weijie Wen,Runping Su,Yunyun Liu,Zhiyang Feng,Yancai Li,Jieli Li,Ziyu Huang,Fen Zhang,Shanshan Gao,Yingfei Ma,Qinghua Zhou,Emad M. El-Omar,Jeremy J. Barr,Tao Zuo
The human gut harbors a diverse virome, believed to play a crucial role in maintaining intestinal homeostasis. However, the magnitude of heterogeneity of the gut mucosal virome, and how it differentially regulates host inflammation phenotypes, such as those observed in Crohn’s disease (CD), remains unclear. Here, we identify two mucosal DNA virome enterotypes (E1 and E2) in humans, with E2 subjects exhibiting higher virome diversity and nuanced bacteriophage-bacteria correlations. Moreover, E2-enterotyped CD patients exhibited higher Wulfhauvirus abundance than healthy controls. The E2 virome is causally more proinflammatory in inducing intestinal inflammation in mice. Mechanistically, the novel E2-virome-enriched phage øBTZT001P (belonging to Wulfhauvirus ) lysogenically infects Bacteroides thetaiotaomicron , increasing sphingosine production. Downstream, sphingosine suppresses the commensal bacterium Blautia obeum in the gut, leading to worsened intestinal inflammation. Our findings reveal that configurational differences in the gut virome may dictate disease outcome through a phage-bacteria-metabolite-intestine axis, highlighting the importance of gut phage-bacteria crosstalk in health.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Science
·
2026
·
IF 14.1
Menghao Qiao,Liqun Zhou,Minhua Zhou,Yu Fang,Haiying Mai,Lingbo Cao,Kun Xu,Yuan Sang,Minyi Chen,Jiewei Huang,Peiyi Huang,Zhipeng Yan,Chao Wang,Zhangshuai Dai,Dichun Huang,Ronghan He,Lijuan Pang,Yunmiao Guo,Ting Gang Chew,Junqi Huang
Ferroptosis is an iron-dependent form of programmed cell death governed by redox homeostasis. Although Ezrin, Radixin, and Moesin (ERM) proteins are established membrane-actin cytoskeleton linkers, their role in ferroptosis remains unexplored. Here, ERM proteins are identified as modulators of erastin-induced ferroptosis. In human fibrosarcoma HT-1080 cells, pharmacological inhibition of ERM phosphorylation, knockdown of individual ERM members, or overexpression of a phospho-deficient Ezrin mutant (T567A) consistently attenuated ferroptosis, whereas wild-type ERM overexpression enhances ferroptosis susceptibility. Mechanistically, ERM inhibition leads to F-actin depolymerization accompanied by a modest rise in reactive oxygen species (ROS). F-actin stabilization prevents this ROS surge and restores ferroptotic sensitivity, whereas its depolymerization mimics the protective effect of ERM inhibition. ROS elevation triggers KEAP1 degradation, stabilizing NRF2 and promoting its nuclear translocation. Activated nuclear NRF2 induces antioxidant genes, particularly HMOX1 , a key effector of heme catabolism that enhances redox buffering and limits lipid peroxidation, ultimately conferring resistance to ferroptosis. The protective effects of ERM inhibition are further validated in ferroptosis-relevant ex vivo and in vivo models. Notably, other pro-oxidants similarly attenuate ferroptosis at appropriate concentrations. Together, these results establish ERM proteins as regulators of ferroptosis and reveal an underappreciated group of ferroptosis inhibitors that engage ROS-NRF2-mediated redox-adaptation.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Polymers
·
2026
·Vol. 18
·期 3
·378
·
IF 4.9
Lin Sun,Zihao Zhang,Bingxin Sun,Zhiheng Yu,Guoyong Wang
Vanadium carbide (V 2 C) MXene shows great potential for addressing challenging implant-associated infections in bone regeneration due to its strong photothermal conversion efficiency. However, its photothermal efficacy is restricted to the near-infrared I (NIR-I) region due to a limited absorption range. To address this, we designed platinum nanoparticle-decorated V 2 C heterostructures (Pt@V 2 C) via an in situ growth method, leveraging Pt’s plasmonic and catalytic properties to extend the photoresponse to the NIR-II window. Subsequently, Pt@V 2 C was integrated into poly-L-lactic acid (PLLA) to fabricate PLLA-Pt@V 2 C scaffolds with photothermal antibacterial function by selective laser sintering. The optimized PLLA-Pt@V 2 C scaffold achieves a record photothermal conversion efficiency (56.03% at 1064 nm), triggering simultaneous hyperthermia (>52 °C) and catalytic ·OH radical generation. In vitro studies demonstrate exceptional antibacterial efficacy against Staphylococcus aureus and Escherichia coli, achieving over 99% killing rates upon 1064 nm near-infrared irradiation. Furthermore, the scaffold demonstrated significant inhibition of biofilm formation, achieving over 90% reduction in biofilm biomass. Moreover, the scaffold demonstrated high cell viability, confirming its dual functionality of potent bactericidal activity and biocompatibility that supports tissue regeneration. This work provides a feasible strategy for combating implant-associated infections.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS ES&T Engineering
·
2026
·
IF 6.3
Linghui Peng,Weina Zhao,Fukai Xu,Manlin Dong,Guiying Li,Weiping Zhang,Zhishu Liang,Taicheng An,Huijun Zhao
The demand for self-cleaning air purification is urgently increasing due to concerns for human health and long-term use. Herein, this study investigated the enhanced mechanism of a self-cleaning artificial spider silk (PMAS) photocatalyst, which was modified by bioaffinity polydopamine (PDA) for highly efficient bioaerosol adhesive capture and photocatalytic inactivation. By increasing the bioaffinity of the PMAS photocatalyst, bioaerosols can adhere to the surface, increasing capture efficiency by 50–700%. The captured bioaerosols were concentrated on the spindle knots of the PMAS photocatalyst, forming droplet reactors for subsequent photocatalytic inactivation. Through a combination of experiments and calculations, the mechanism of enhanced capture efficiency by the PMAS photocatalyst was revealed: PDA endows doubled adsorption energy and tripled adhesion forces between the interfaces of bioaerosols and the photocatalyst due to the abundant indole and amino groups. PDA also provides hydrophilic properties to the PMAS photocatalyst, leading to a sevenfold increase in captured bacterial droplet volumes. Due to its bioaffinity, hydrophilic, and photocatalytic properties, the PMAS photocatalyst possesses self-cleaning capability by in situ inactivation of microorganisms and purification of self-washed debris, facilitating repeated use for long-term capture and regeneration of occupied active sites. This work may offer rational designs for a new generation of self-cleaning photocatalysts for air purification.
✓ 已验证
产品关联
DOI
高影响因子
Small
·
2026
·
IF 11.8
Yiyan Yang,Linjun Zhang,Jianhua Zhang,Rong Zhang,Hengjie Zhang,Maoyun Li,Zhipeng Gu,Yiwen Li,Lei Yang
The physical eradication of bacterial biofilms is fundamentally limited by rapid post-treatment regeneration. A strategy that integrates physical destruction with biological suppression is therefore highly coveted. Herein, we introduce a strategy to chemically reprogram magnetic liquid metals (LMs) into intelligent antibiofilm nanocomposites. Through a one-step metal-phenolic coordination, we coat the LM with a natural polyphenol (baicalin, BA), creating a nanoplatform (MBA) that executes a synergistic “destroy-and-pacify” mission. This design establishes a self-reinforcing loop: magnetic actuation provides the mechanical force to breach the biofilm matrix, enhancing the penetration of the BA coating. Concurrently, the BA-mediated inhibition of quorum sensing and EPS synthesis pre-weakens the biofilm, making it more susceptible to physical ablation. This reciprocal potentiation leads to exceptional efficacy, more than doubling the clearance efficiency of mature P. aeruginosa biofilms on implants in a murine model and achieving a level of eradication unattainable by monotherapies. This work establishes a new paradigm for designing smart theranostic microrobots, paving the way for programmable platforms to tackle complex biological barriers.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2026
·
IF 7.8
Kunkun Tu,Muze Han,Jinjing Liu,Ronghua Yu,Jiayi Li,Jie Wang,Suhao Li,Yifu Zhu,Na Wang,Huan Xu,Xue Luan,Xueyu Pan,Kaimin Hua,Hao Jin,Shuda Hu,Xingyun Li,Wenjie Ge,Shihang Li
Wood is a sustainable carbon-storage material that is widely used in construction and packaging. However, its intrinsic flammability restricts its application. Existing flame-retardant modifications either rely on toxic chemicals or generate byproducts. In contrast, bioinspired microbially induced calcium carbonate precipitation offers a solution to this challenge. In this study, a facile method was developed for the in situ growth of Bacillus mucilaginosus (B.m.) on the wood porous structure. The B.m. solution exhibited a significantly low surface tension and a favorable wettability on wood surfaces. This is attributed to the extracellular polymeric substances secreted by the strain, which promote its deep penetration into wood structures without relying on vacuum or pressure-assisted conditions. During ambient drying, B.m. utilizes carbonic anhydrase to capture atmospheric CO2, inducing CaCO3 deposition in cell lumens of wood and formed mineralized wood. Morphological and chemical characterizations confirmed the formation of well-crystallized CaCO3 without altering the wood appearance. The B.m.-treated wood has a higher residual weight, delayed thermal decomposition, reduced heat release, and faster self-extinguishing ability, achieving enhanced flame retardancy. This waste-free and energy-efficient process can achieve CO2 sequestration, aligns with zero-waste city goals, and has broad potential in fire-safe wood applications.
✓ 已验证
产品关联
DOI
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2026
·
IF 3.5
Ji Runzhen,Hua Qijing,Dong Yamin,Zhu Du,Xiao Yiwen,Gao Boliang
Xylanase is the core enzyme responsible for degrading xylan, a major component of lignocellulose, and holds significant application value in fields such as food, feed, and biorefining. In this study, a xylanase gene, Xyn108 , was identified from the genome of the lignocellulose-degrading endophytic fungus Chaetomium globosum DX-THS3 and successfully expressed in Pichia pastoris . The xylanase Xyn108 belongs to glycoside hydrolase family 11 (GH11), with a molecular weight of approximately 38 kDa. The enzyme exhibited optimal activity at pH 6.0 and 40 °C, and demonstrated good stability within pH 3.0–9.0 and at temperatures below 50 °C (retaining > 50% residual activity after 180 min incubation). Xyn108 displayed high enzymatic activity towards beechwood xylan and possessed hydrolytic capacity toward microcrystalline cellulose. Crucially, Xyn108 exhibited significant synergistic effects with the commercial cellulase Cellic CTec2 in hydrolyzing pretreated rice straw. The highest synergistic degrees, reaching 1.34 and 1.24 for NaOH-pretreated and H₂SO₄-pretreated straw, respectively, were achieved at a xylanase/cellulase activity ratio of 1:3. The enzyme’s excellent pH adaptability, low-temperature activity, and remarkable synergistic effects highlight its significant application potential in industrial sectors such as feed processing, paper manufacturing, and biofuel production.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
·
2026
·
IF 7.1
Mengru Wang,Biao Zhang,Wen-Sheng Zou,Jinhui Zhang,Yaqin Wang
A self-cleaning ultrafiltration membrane with electrically switchable antifouling and antibacterial functions was developed by incorporating a g‑C 3 N 4 /reduced graphene oxide (rGO) composite into a polyvinylidene fluoride (PVDF) matrix. At + 1.5 V, the optimized membrane (M3, 7.5 wt% g‑C 3 N 4 ) achieved a pure water flux of 115.97 L·m −2 ·h −1 , and a BSA flux retention 85.6 % (51.35 L·m −2 ·h −1 ) after 3 h, outperforming the control membrane (M0) with 76.57 L·m −2 ·h −1 , and 59.2 % retention (35.53 L·m −2 ·h −1 ). The M3 membrane achieved a BSA rejection of 99.70 %, a flux recovery ratio (FRR) of 93.86 %, and an antibacterial efficiency of ∼99.7 % against E. coli under dark conditions. The superior self-cleaning performance is attributed to the synergistic effects of enhanced electrostatic repulsion and electrocatalytic generation of reactive oxygen species (ROS). This work offers a practical strategy for designing smart separation membranes with on-demand antifouling and self-cleaning capabilities for advanced water purification.
✓ 已验证
产品关联
DOI
高影响因子
FOOD CONTROL
·
2026
·
IF 7
Jingbo Sun,Liushunhao Chen,Hongchen Li,Xiaobo Liu,Ling Lin,Lei Meng,Tuanhui Chen,Qian Wen,Dong Xu,Jiali Ren
Effectively controlling spoilage remains a critical challenge for maintaining safety and quality of food products. Here, we tested the hypothesis that carbonization of monostearin, a safe but weak antimicrobial emulsifier, can yield monostearin-derived carbon dots (MCDs) with enhanced antibacterial properties suitable as coating ingredients for food preservation . Although the hydrothermally synthesized MCDs are composed solely of C, O, and H, they exhibit strong antibacterial activity against E. coli and S. aureus, with minimal inhibitory concentrations as low as 0.01 mg/mL and 0.08 mg/mL, respectively. Mechanistic studies revealed that MCDs disrupt the structural integrity of both bacterial species and interfere with cellular metabolism. RT-qPCR analysis showed both upregulation and downregulation of gene expression in E. coli , whereas only downregulation occurred in S. aureus . For practical applications, MCDs were dispersed in alginate solution as the coating solutions, providing a safe and effective platform for extending shelf life of strawberries and meats. After 8 days of storage, the MCDs-alginate coating significantly reduced absolute weight loss relative to the control, from 37.4% to 26.7% in strawberries and from 16.4% to 11.8% in pork, while lowering the absolute rotting rate from 83.3% to 33.4% and from 66.3% to 37.6%, respectively. These improvements effectively preserved key sensory attributes, including color, odor, texture, and overall acceptability. This work establishes a strategy for engineering non-preservative food additives into antimicrobial agents, opening avenues for developing next-generation food preservatives.
✓ 已验证
产品关联
DOI
高影响因子
Foods
·
2026
·Vol. 15
·期 5
·829
·
IF 6
Jinqiong Xu,Xinyu Yu,Jianbo Liu,Qinglin Sheng
Pseudomonas aeruginosais an important waterborne pathogen that is harmful to food safety and human health. Therefore, the detection ofPseudomonas aeruginosahas become an essential item in food sampling. This article constructs a fluorescent aptamer sensor based on Tb-MOFs, using Tb-MOFs as the fluorescence signal source, colloidal gold (AuNPs) as the signal conversion switch, and aptamers as the target recognition element, to establish a quantitative detection method forPseudomonas aeruginosa. The constructed sensor exhibits a good linear relationship within the concentration range ofPseudomonas aeruginosafrom 1 to 106CFU/mL, with a detection limit of 0.63 CFU/mL. Moreover, the sensor was also applied to the detection ofPseudomonas aeruginosain actual samples of bottled drinking water and orange juice. The fluorescent aptasensor based on Tb-MOFs provides a rapid and sensitive new sensing method for the detection ofPseudomonas aeruginosain food.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Small
·
2026
·
IF 11.8
Yitian Zhang,Dianji Li,Minhao Liu,Ziqing Tang,Hui Yu,Min You,Qiang Chen,Xi Ding
Highly wet, dynamic, inflammatory, and fouling environments were significant challenges in the healing of diabetic oral ulcers. Inspired by the oral mucosa, a wet-adhesion Janus hydrogel (WAJH) with asymmetric bifunctionalities of antifouling/antioxidant was proposed for diabetic oral ulcer treatment. In the WAJH, the antifouling layer was composed of the agar/polyacrylamide double-network (Agar/PAAm DN) hydrogel, and the wet-adhesive layer was constructed by the poly(acrylic acid-co-2-phenoxyethyl acrylate)/tannic acid (PAEA/TA) hydrogel. The antifouling layer mimicked the oral epithelium to prevent pollutant invasion, and the adhesive layer imitated the basement membrane of oral mucosa to adhere firmly to wet diabetic oral ulcers. Importantly, the adhesion energies of the two layers were distinctly different, measuring 15 J m −2 for the antifouling layer and 316 J m −2 for the adhesive layer, respectively. Notably, TA not only significantly enhanced the toughness and wet adhesion but also endowed the WAJH with reactive oxygen species (ROS) scavenging and antioxidant capabilities. The WAJH remarkably accelerated the healing of oral ulcer by inhibiting inflammation at the ulcer, promoting angiogenesis, and promoting re-epithelialization in vivo. Consequently, the WAJH with antifouling and antioxidant exhibited promising prospects in the clinical treatment of diabetic oral ulcer.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）