APPLIED AND ENVIRONMENTAL MICROBIOLOGY
·
2025
·
IF 3.7
Zhili Shi,Ya Li,Weibo Shi,Zhixin Mu,Qingxi Han,Weiwei Zhang
Probiotics are prior agents for treating bacterial infection with advantages of inhibiting pathogenic bacteria and improving immune responses of hosts, thus increasing the survival rate of cultured animals. In this study, one Vibrio parahaemolyticus YDE17 pathogenic to shrimp and its antagonist Glutamicibacter sp. ZY1 were screened, and ZY1 showed stable inhibitory effects on diverse Vibrio spp., especially V. parahaemolyticus. ZY1 secreted inhibitory substances into supernatant, and the activity of inhibitory substances did not change after being treated under different temperatures, proteinase K, and pH (6-10), which indicated that the inhibitory substances might be small molecules, which led us to trace the siderophore production. The siderophore production of YDE17 co-incubated with the cell-free supernatant of ZY1 was greater than that of YDE17 alone, which indicated that the cell-free supernatant of ZY1 created iron-limiting conditions for YDE17. This finding was confirmed by iron supplementation assays, in which the inhibitory activity of the cell-free supernatant of ZY1 on YDE17 as well as the siderophore production of YDE17 decreased in the presence of FeCl3. The effect of iron on inhibition was further confirmed by in vivo infection. The relative percent survival of ZY1 to shrimp challenged by YDE17 was 83.3%, but the survival rates of shrimp challenged with YDE17/ZY1/FeCl3 were similar to that of YDE17, both of which were significantly lower than the 70% survival rate of shrimps simultaneously challenged by ZY1/YDE17. Our study offers a new probiotic resource to control vibriosis, which works through iron competition with the opportunistic pathogens of Vibrio spp.
研究领域:
微生物学
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水产养殖
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病原生物学
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产品关联
DOI
PubMed
高影响因子
APPLIED CATALYSIS A-GENERAL
·
2025
·
IF 4.8
Xiao Du,Tengfei Li,Lumei Wang,Dezhang Ren,Wenhui He,Zhibao Huo
In this paper, an alkali-free route for the conversion of chitin and shrimp shell to acetic acid (AA) using aluminum salts in aqueous media was reported for the first time. The proposed method demonstrated several advantages, including simplicity, effectiveness, and superior environmental and economic benefits compared to previous approaches. Notably, it achieved a significant breakthrough in the yields of AA derived from chitin and shrimp shells. Under the optimal conditions, the yield of AA reached the maximum value of 81.4 %. Combined with theoretical calculation and characterization, a possible transformation mechanism was proposed, and it was proved that the presence of aluminum salts would change the main reaction pathway and participate in the preparation of AA in the form of polymers. This study provided a novel idea for the resource utilization of chitin and waste shell materials into valuable chemicals and also for the treatment of environmental pollution.
研究领域:
生物化学
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环境科学
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材料科学
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产品关联
DOI
高影响因子
Marine Drugs
·
2020
·Vol. 18
·期 10
·492
·
IF 4.073
Jiawen Zhao,Kening Jiang,Yixuan Chen,Juan Chen,Yangfan Zheng,Huilin Yu,Jiajin Zhu
Antarctic krill oil is high in nutritional value and has biological functions like anti-inflammation and hypolipidemic effects. But it has and unpleasant smell, and unsaturated fatty acids are prone to oxidative deterioration. Its high viscosity and low solubility in water make it difficult for processing. Microemulsion can be a new promising route for development of krill oil product. We determined a formula of krill oil-in-water microemulsion with krill oil: isopropyl myristate = 1:3 as oil phase, Tween 80:Span 80 = 8:2 as surfactant, ethanol as co-surfactant and the mass ratio of surfactant to co-surfactant of 3:1. After screening the formula, we researched several characteristics of the prepared oil-in-water microemulsion, including electrical conductivity, microstructure by transmission electron microscope and cryogenic transmission electron microscope, droplet size analysis, rheological properties, thermal behavior by differential scanning calorimeter and stability against pH, salinity, and storage time.Keywords:microemulsion;Antarctic krill oil;formula selection;characterization
研究领域:
食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
FISH & SHELLFISH IMMUNOLOGY
·
2019
·
IF 3.298
Yafei Duan,Yun Wang,Dalin Xiong,Jiasong Zhang
The intestine is important for nutrition, metabolism and immunity. Succinate (SA) plays a vital role in the physiological homeostasis of animal intestines. However, the effects of dietary SA on the intestinal immunity and metabolism in shrimp are not clear. In this study, we investigated the immune and metabolic responses in the intestine of Litopenaeus vannamei that were fed diets consisting of different levels of SA: 0 g/kg (Con) and 10 g/kg (SA) for 56 days. The results from a RNA-seq analysis identified 6005 differentially expressed genes (DEGs), including 2728 upregulated genes and 3277 downregulated genes, which were grouped into 312 pathways. The DEGs were most enriched in pathways related to protein synthesis and amino acid metabolism , including “ribosome”, “aminoacyl-tRNA biosynthesis”, “pyrimidine metabolism”, and “arginine and proline metabolism”; additionally, carbohydrate and lipid metabolism pathways were also activated. A large number of immune-related genes were associated with mucus barrier modification, antimicrobial activity , pathogen attachment and recognition, antioxidant activity , and apoptosis. The expression patterns of several candidate genes involved in the immune response and nutrition metabolism were detected by qPCR. This study provides insight into the transcriptomic modulating mechanisms associated with intestinal immunity and the metabolism of L. vannamei in response to the intake of dietary SA.
研究领域:
水产养殖学
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分子生物学
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营养学
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产品关联
DOI
PubMed
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Yang Liu,Zejun Zhang,Yushu Li,Fan Shi,Yijing Ai,Baoli Wang,Siyue Zhang,Xiaoping Zhang,Wei Sun
An electrochemical sensor based on marine biomass shrimp shell-derived porous carbon (SSPC) modified glassy carbon electrode (GCE) was developed for the detection of hydroquinone (HQ) in this paper. The resultant SSPC was characterized by different methods including scanning electron microscopy and Fourier transform infrared spectroscopy. Brunauer-Emmett-Teller method was used to evaluate the pore structure and surface area of SSPC. The electrochemical behaviors of SSPC/GCE were explored by cyclic voltammetry and differential pulse voltammetry. The SSPC/GCE demonstrated linear response ranges of 5.0–25.0 μM and 25.0–1200.0 μM for HQ analysis with the limit of detection as 0.18 μM (S/N = 3). Additionally, the method had been successfully applied to determine HQ in real lake water sample, which provided potential applications in further sensing study.
研究领域:
材料科学
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环境科学
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分析化学
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产品关联
DOI
高影响因子
Biomolecules
·
2023
·Vol. 13
·期 3
·496
·
IF 5.5
Yingyuan Zhao,Zhaoxuan Wang,Shuxian Liu,Shiying Xie,Yinchun Xie,Ruifang Li,Hiroaki Oda
Astaxanthin is a carotenoid with excellent antioxidant activity. However, this small lipid-soluble molecule is insoluble in water and has low stability. Although this situation can be improved when astaxanthin is prepared as a nanosuspension, the aqueous form is still not as convenient and safe as the dry powder form for storage, transport, and use. The lyophilization process provides better protection for thermosensitive materials, but this leads to collapse and agglomeration between nanoparticles. To improve this situation, appropriate lyophilization protectants are needed to offer support between the nanoparticles, such as sugars, amino acids, and hydroxy alcohols. The purpose of this work is to screen lyophilization protectants by single-factor experiments and response surface optimization experiments and then explore the optimal ratio of compound lyophilization protectants, and finally, make excellent astaxanthin/BSA/chitosan nanosuspension (ABC-NPs) lyophilized powder. The work shows that the optimal ratio of the compounding lyophilization protectant is 0.46% oligomeric mannose, 0.44% maltose, and 0.05% sorbitol (w/v). The ABC-NPs lyophilized powder prepared under the above conditions had a re-soluble particle size of 472 nm, with a ratio of 1.32 to the particle size of the sample before lyophilization. The lyophilized powder was all in the form of a pink layer. The sample was fluffy and dissolved entirely within 10 s by shaking with water. Consequently, it is expected to solve the problem of inconvenient storage and transportation of aqueous drugs and to expand the application of nanomedicine powders and tablets.Keywords:lyophilization;astaxanthin nanoparticles;freeze-drying protectant
研究领域:
药学
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纳米技术
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生物技术
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2023
·
IF 11.2
Qingqing Tao,Felipe Nunes Henriquez,Kang Ding,Wai Lun Man,Matthew Y. Lui
The application of ionic liquids and deep eutectic solvents offers a promising opportunity for a more environmentally friendly and straightforward chitin purification process from crustacean shells. Nonetheless, the insufficient recyclability of these ionic solvents poses a challenge to the long-term sustainability of such extraction methods. Thus, there is a strong imperative to focus on employing easily recyclable ionic liquids for chitin isolation, enhancing the overall sustainability of the process. In this investigation, a direct chitin purification procedure that utilized pulping liquors consisting of the superbase-based protic ionic liquid 1,5-diazabicyclo[4.3.0]non-5-enium acetate and its precursor, acetic acid, was developed. It was demonstrated that these pulping liquors were capable of simultaneously deproteinate and demineralize shrimp shells to generate chitins with higher purity, degree of N -acetylation and crystallinity than commercially obtained chitin. More significantly, the pulping liquors can be recycled to their pure form in high quantity by simple distillation under reduced pressure, allowing the reuse of these mixtures, which give chitin of nearly identical purity.
研究领域:
生物化学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
AQUACULTURE
·
2022
·
IF 5.135
Jia Liu,Cheng Chen,Hui Du,Dexin Wang,Haixiang Ma,Gaoxue Wang,Tao Liu,Erlong Wang
White spot syndrome virus (WSSV), the causative agent of White spot disease (WSD), has caused high mortality and great economic losses in worldwide crustacean industries including shrimp and crayfish. Many bioactive components of Chinese herbs have been reported to be effective to control aquatic viral diseases including WSD. In this study, the antiviral effects of twelve herbal extracts were evaluated in WSSV-infected crayfish model, and Sophora flavescens extract exhibited the highest inhibition rate (87.63%) of WSSV replication. The anti-WSSV effect of matrine , the main bioactive component of S. flavescens, was further investigated. The results showed matrine could significantly inhibit WSSV replication in a dose-dependent manner and exhibited a robust inhibition rate (96.16%) with 50 mg/kg. Matrine both possessed potential preventive and therapeutic effects against WSSV infection and the preventive effect was better than the therapeutic effect. Besides, compared with the control, matrine significantly ( p < 0.01) improved the survival rate of WSSV-infected crayfish (from 0% to 48.57% on 10th day), notably ( p < 0.01) suppressed the expression of virus replication-related genes ie1 , DNApol and Vp28 , remarkably ( p < 0.01) activated C-type lectin ( CTL ) and prophenoloxidase ( ProPo ) and inhibited barrier-to-autointegration ( BAF ) expression to improve crayfish innate immunity against WSSV infection, up-regualated the CAT and GST expression and down-regulated the expression of NF-κB , cyclooxygenases COX-1 and COX-2 genes to balance the oxidative stress and inflammatory injury caused by WSSV. This study will provide a theoretical basis for the development of matrine as an efficient and practical anti-WSSV drugs and meaningful reference for the study of antiviral effect of matrine on other aquatic animal viruses.
研究领域:
水产养殖
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病毒学
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免疫学
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药理学
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DOI
高影响因子
CURRENT MICROBIOLOGY
·
2025
·Vol. 82
·期 9
·1-11
·
IF 2.6
Gu Yiwen,Lin Xiu,Lu Yanxu,Fang Shuguang,Hu Kun,Ju Xin,Li Liangzhi,Chen Zhi
The increasing use of antibiotics in shrimp aquaculture has raised concerns regarding ecological safety and the emergence of antibiotic resistance. This study aimed to isolate and characterize probiotic lactic acid bacteria (LAB) strains from the gut of Penaeus vannamei to evaluate their potential as antibiotic alternatives in aquaculture. Two strains, S1 and S2, were identified with 99.79 and 99.51% similarity to Pediococcus acidilactici DSM 20284 and Enterococcus lactis BT159, respectively. Based on minimum inhibitory concentration (MIC) assays, both strains exhibited high sensitivity to the tested antibiotics. They also demonstrated notable acid and bile salt tolerance, with S1 maintaining a survival rate of 30% at pH 5 and S2 achieving 40% survival at pH 6. Under 0.03% bile salt concentration, survival rates were approximately 60% (S1) and 45% (S2), while S1 retained 15% viability even at 0.9% bile salt. Enzymatic activity analysis indicated that both strains exhibited lower amylase activity compared to other hydrolytic enzymes, with S2 showing higher cellulase and protease activities than S1. Antimicrobial assays revealed that both strains exerted broad-spectrum inhibition against four indicator pathogens. S1 exhibited the strongest inhibitory effect against Aeromonas hydrophila (inhibition zone: 1.6–2.2 cm), whereas S2 showed the highest inhibition against Vibrio parahaemolyticus (1.5–2.4 cm). Feeding trials demonstrated that the combined application of S1 and S2 significantly enhanced shrimp body weight (6.05 ± 0.09 g vs. 9.23 ± 0.2 g, P < 0.01) and survival rate (62.49% ± 1.56 vs. 93.78% ± 2.55, P < 0.01). These findings highlight the potential of Lactobacillus strains as probiotics in shrimp farming, offering a sustainable approach to reducing antibiotic dependency and improving shrimp health and productivity.
研究领域:
水产养殖
·
微生物学
·
应用微生物学
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益生菌研究
·
抗生素耐药性
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海洋生物学
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动物营养
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产品关联
DOI
PubMed
高影响因子
AQUACULTURE NUTRITION
·
2020
·Vol. 26
·期 3
·978-989
·
IF 2.231
Shi-wei Xie,Dan Wei,Shi-jun Chen,Zhenxiao Zhuang,Peng Yin,Yong-jian Liu,Li-xia Tian,Jin Niu
In this manuscript, three iso-nitrogenous and iso-lipidic diets containing 50, 150 and 250 g/kg of fishmeal (FM) were fed white shrimp Litopenaeus vannamei for 8 weeks. At the end of feeding, survival and percentage weight gain of shrimp were examined. Hepatopancreas and haemolymph were sampled, activities of superoxide dismutase (SOD) and catalase (CAT), nitric oxide (NO), glutathione (GSH) and malondialdehyde (MDA) levels in hepatopancreas, activities of SOD, CAT, acid phosphatase (ACP) and alkaline phosphatase (AKP), and NO, GSH, MDA in haemolymph were examined. Haemolymph was further subjected to GC-MS analysis. Results indicated that no significant differences in survival rate and percentage weight gain were observed among three treatments. SOD activity and GSH levels in hepatopancreas, and CAT activity, AKP activity, GSH and MDA levels in haemolymph were significantly lower in shrimp fed 50 g/kg FM diet. GC-MS analysis of haemolymph indicated that 81 metabolites were significantly altered in the three groups. Furthermore, 8 metabolism pathways were significantly influenced by dietary FM levels. In conclusion, dietary administration of 50 g/kg FM resulted in inhibition of antioxidant ability, and disordered the energy metabolism, one-carbon metabolism and unsaturated fatty acid metabolism in shrimp.
研究领域:
水产养殖
·
营养学
·
生物化学
·
代谢
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产品关联
DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2019
·
IF 6.669
Yu-Hong Liu,Adili Alimujiang,Xiang Wang,Shan-Wei Luo,Srinivasan Balamurugan,Wei-Dong Yang,Jie-Sheng Liu,Lin Zhang,Hong-Ye Li
Haematococcus pluvialis is a main biological resource for the antioxidant astaxanthin production, however, potential modulators and molecular mechanisms underpinning astaxanthin accumulation remain largely obscured. We discovered that provision of ethanol (0.4%) significantly triggered the cellular astaxanthin content up to 3.85% on the 4th day of treatment. Amongst, 95% of the accumulated astaxanthin was esterified, particularly enriched with monoesters. Ultrastructural analysis revealed that ethanol altered cell wall structure and physiological properties. Antioxidant analyses revealed that astaxanthin accumulation offset the ethanol induced oxidative stress. Ethanol treatment reduced carbohydrates while increased lipids and jasmonic acid production. Transcriptomic analysis uncovered that ethanol orchestrated the expression of crucial genes involved in carotenogenesis, e.g. PSY, BKT and CRTR-b were significantly upregulated. Moreover, methyl jasmonic acid synthesis was induced and played a major role in regulating the carotenogenic genes. The findings uncovered the novel viewpoint in the intricate transcriptional regulatory mechanisms of astaxanthin biosynthesis.
研究领域:
植物学
·
分子生物学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2020
·
IF 3.721
Qing Shen,Gongshuai Song,Honghai Wang,Yanping Zhang,Yiwei Cui,Hujun Xie,Jing Xue,Haixing Wang
Shrimp waste contains a large number of bioactive lipids for preparing functional foods. In this study, a 1,2-dichloroethane based method for extracting phospholipids from shrimp waste was developed. The number of phospholipids recovered by 1,2-dichloroethane/methanol at a ratio of 1:2 (v/v) was 10.97 g/100 g, which was comparable with the performance of conventional methods. The lipidomics profile of the extract from shrimp waste was studied through gas chromatography with flame ionization detection (GC/FID) and hydrophilic interaction liquid chromatography coupled to QTrap mass spectrometry (HILIC-QTrap/MS) to reveal the effect of different extraction methods on the selectivity of lipids. It was found that the lipid extracts using different methods showed significant variation, while the diversity and percentage of each molecular species were generally the same. Besides, a large number of health beneficial dietary ω-3 polyunsaturated fatty acid (PUFA) structured phospholipids were detected, which indicated that shrimp waste was an important source with significant nutritional values.
研究领域:
食品科学
·
生物化学
·
营养学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）