BIOSENSORS & BIOELECTRONICS
·
2023
·
IF 12.6
Hao Bai,Yuqing Liu,Linbo Gao,Tao Wang,Xiaoli Zhang,Jie Hu,Lisha Ding,Yueting Zhang,Qian Wang,Lei Wang,Jianlong Li,Zhifeng Zhang,Yang Wang,Chenlan Shen,Binwu Ying,Xiaoyu Niu,Wenchuang Hu
Screening for high-risk human papillomavirus (HPV) infection is one of the most important preventative measures for cervical cancer. However, fast, convenient, and low-cost HPV detection remains challenging, especially in resource-limited settings. Here, we report a portable all-in-one device (PAD) for point-of-care testing (POCT) for HPV16 and HPV18 DNA in cervical swabs. The PAD was engineered to integrate modules for extraction-free sample lysis, loop-mediated isothermal amplification (LAMP) with lyophilized reagent beads, and real-time colorimetric signal sensing into a single miniaturized device, considerably shortening the sample-to-result time to 15 min. The precision liquid handling in the completely sealed microfluidic chip is achieved by a uniquely designed pressure-balanced automatic liquid flow mechanism, thereby eliminating the need for manual manipulation of liquids and thus the risk of biohazards . The PAD employs an improved real-time colorimetric LAMP (rcLAMP) assay with a limit of detection (LOD) of 1 copy/μL, enabled by enhanced assay chemistry to maximize the reaction kinetics . To validate this device for clinical application, we tested 206 clinical cervical swab samples and obtained a sensitivity of 92.1% and a specificity of 99.0%. This custom PAD enabled by microfluidic and electronic engineering techniques can be configured for the simultaneous detection of HPV16 and HPV18 or other pathogens in point-of-care applications.
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高影响因子
AMB Express
·
2018
·Vol. 8
·期 1
·1-12
·
IF 1.719
Hu Xingcui,Cheng Tao,Liu Jianhua
Biosurfactants emulsify lipids, facilitating their exposure to microorganisms in water. Secretion of biosurfactant together with lipase can enhance oil assimilation by microorganisms. In this study, we show the analysis of the biosurfactant and lipase producing microbe originating from oil-sludge. The enrichment of the biosurfactant-producing ZS6 strain from the mixed culture of sludge-derived microbes in medium with olive oil as sole carbon source was monitored using the modified T-RFLP (or mT-RFLP) methodology. Phylogenetic tree analysis based on 16S rDNA sequences suggested that ZS6 belonged to a member of Serratia sp. Serratia sp. ZS6 secreted both a serrawettin-type biosurfactant and lipase in medium with olive oil as sole carbon source. By using an in-gel lipase assay followed by LC–MS/MS analysis, we identified the amino acid sequences of the ZS6 lipase, which belonged to the lipase subfamily III of the family I. Its lipolytic activity was found to be enhanced by salinity, calcium, and methanol. Together, we show that the novel isolate Serratia sp. ZS6 secretes both biosurfactant and lipase which makes it useful for applications such as in food industry wastewater treatment and biodiesel production.
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ANALYST
·
2018
·Vol. 143
·期 12
·2901-2907
·
IF 3.864
Wenyue Xie,Lingli Lei,Meiling Tian,Zeying Zhang,Yingshuai Liu
A high-resolution colorimetric immunoassay platform has been developed based on enzyme-catalyzed multicolor generation and smartphone-assisted signal readout. The multi-color generation is accomplished in this system through the urease-catalyzed urea hydrolysis-induced color change of the pH indicator phenol red, from yellow to orange to red over pH 6.6 to 8.0. The color change is easily tailored by controlling the urease activity via the inhibitor silver ion (Ag+), the amount of which is in turn adjusted by alkaline phosphatase (ALP)-catalyzed ascorbic acid (AA) production. An ALP-linked colorimetric immunoassay is readily realized based on the above urease catalyzed multicolor generation system. Under optimal conditions, a limit of detection (LOD) of 1.73 ng mL−1 and a dynamic range from 0 to 18 ng mL−1 are achieved with rabbit IgG as a model analyte. A colored picture for each test is directly taken using a smartphone and then quantitatively analyzed with the free software ImageJ, eliminating the use of expensive and desktop equipment. The dose-dependent multicolor display is easier to distinguish with the naked eye for qualitative or semiquantitative detection over the traditional one-color system. The developed immunoassay scheme provides a promising platform for on-site testing or applications in resource-poor areas.
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Biomaterials Science
·
2024
·
IF 5.8
Hui Zhi,Yingxi Qin,Yang Li,Fengya Wang,Liang Feng
As water-saturated polymer networks, the easy water loss of hydrogels directly affects their end-use applications. Minimizing the ratio of free water and increasing the ratio of bound water in the gel system has become key to extending the service life. In this work, an ionogel is prepared that effectively regulates the proportion of free water and bound water through the formation of wrinkle angles by the hydrophilic and hydrophobic chains in the gel system and the non-volatile nature of the ionic liquid. Acrylamide and N-acryloyl phenylalanine are used as free radical comonomers, and phenol red is used as an acid–base indicator. The ionic liquid is used as a dispersant to stabilize the whole framework. Due to the hydrogen bonding interactions, electrostatic interactions, and ion–ion interactions, the ionogel exhibits good stretchability, adhesion, pH sensitivity, and stability. The ionogel can be stretched in multiple directions without cracking and can be bent 180° after being left in air for 45 days. Assembling the ionogel into a wearable device can effectively monitor the pH value of sweat during exercise. The detection results are displayed in the form of RGB values, providing a preliminary diagnosis of the health of the human body.
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ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Yanshu Xiang,Yongjie Zhao,Yifan Jiang,Jiarui Feng,Xiaoyi Zhang,Wei Su,Yingjie Zhou,Peng Wei,Sze Shin Low,Hao Nan Li
The sophisticated environment of chronic wounds, characterized by prolonged exudation and recurrent bacterial infections, poses significant challenges to wound recovery. Recent advancements in multifunctional wound dressings fall short of providing comprehensive, accurate, and comfortable treatment. To address these issues, a battery-free and multifunctional microfluidic Janus wound dressing (MM-JWD) capable of three functions, including exudate management, antibacterial properties, and multiple indications of wound infection detection, has been developed. During the treatment, the fully soft microfluidic Janus membrane not only demonstrated stable unidirectional fluid transport capabilities under various skin deformations for a longer period but also provided antibacterial effects through surface treatment with chitosan quaternary ammonium salts and poly(vinyl alcohol). Furthermore, integrating multiple colorimetric sensors within the Janus membrane’s microchannels and a dual-layer structure enabled simultaneous monitoring of the wound’s pH, uric acid, and temperature. The monitoring was facilitated by smartphone recognition of color changes in the sensors. In vivo and in vitro tests confirmed the exudate management, antibacterial, and sensing capabilities of the MM-JWD, proving its efficacy in monitoring and promoting the healing of wounds. Overall, this study provides a valuable method for the design of multifunctional wound dressings for chronic wound care.
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Chemical Engineering and Processing-Process Intensification
·
2025
·
IF 3.9
Meng-Meng Du,Yuan-Cheng Wang,Bao-Chang Sun,Yong Luo,Liang-Liang Zhang,Guang-Wen Chu,Hai-Kui Zou
Carbonic anhydrase (CA) is a high-efficiency biocatalyst that significantly improves the absorption of CO 2 by tertiary amine. This work aims to investigate kinetics behaviors from the perspective of enzymatic reaction mechanism. The influences of the CA concentration, type of tertiary amines, pH, and temperature on the reaction rate between CO 2 and tertiary amine ( ν ) and catalytic activity of CA ( φ ) were first investigated in a stopped-flow device. Adding 50 g∙m⁻³ CA enhanced ν in tertiary amine solutions by a factor ranging from 22 to 42 at 298 K and pH=9.5, demonstrating its excellent catalytic performance. The ν increased with increasing CA concentration, pH, temperature, and tertiary amine's pK a. φ increased with the increase of CA concentration, as well as the decrease of temperature, pH, and tertiary amine's pK a. Proteomics analysis further revealed that conformational changes of the CA's secondary structure induced by high pH and temperature altered the expressions of the local active-site region and deactivated CA, ultimately leading to a decrease in φ . Additionally, the CA-catalysis kinetics equation accorded with the Michaelis-Menten model, with catalytic second-order rate constants on the magnitude of 10 7 . Overall, this work provides a guideline for its industrial application in the CO 2 capture process.
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JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
·
2025
·
IF 6
Meinan Yang,Nan Wang,Xu Wang,Ruiyong Zhang,Ini-lbehe Nabuk Etim,Mei-E. Yue,Jizhou Duan,Baorong Hou,Wolfgang Sand
Oxygen vacancies can provide active sites for catalysts, promoting catalytic activity. However, there are fewer nanozyme catalysts with oxygen vacancies. In this study, CeO 2 metal–organic framework (CeO 2 -MOF) nanorods with oxygen vacancies are synthesized through a simple hydrothermal and calcination processes. The obtained CeO 2 -MOFs exhibit excellent nanozyme catalytic activity for antibacterial applications. The CeO 2 -MOF nanorods demonstrate intrinsic haloperoxidase-like activity by catalyzing the bromination of organic signaling compounds, outperforming the catalytic properties of pure CeO 2 . The activity of CeO 2 -MOF (6) is the best because of its large specific surface area of 103.638 m 2 /g, surpassing that of CeO 2 at 74.752 m 2 /g. The oxygen vacancies are crucial in regulating the electronic structure of CeO 2 -MOFs, thereby improving the catalytic performance. The experiments of multiple substrates show that CeO 2 -MOF (6) exhibits good stability and recyclability. Additionally, CeO 2 -MOF (6) can catalyze the oxidation of Br - with H 2 O 2 to the corresponding hypobromous acid (HBrO), which exhibits strong antibacterial activity against typical marine bacteria ( Pseudomonas aeruginosa ). This study expands the range of biomimetic enzyme materials. It provides an efficient and green perspective and strategy for developing antibacterial and antifouling biomimetic catalysts, which offers a sustainable alternative for industrial applications in marine coatings and environmental protection.
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JOURNAL OF CONTROLLED RELEASE
·
2017
·
IF 7.786
Jingjing Hu,Yanchun Quan,Yuping Lai,Zhao Zheng,Zhiqi Hu,Xinyu Wang,Tianjiao Dai,Qiang Zhang,Yiyun Cheng
Aminoglycosides are a family of critically important antibiotics for the treatment of serious infections including multidrug-resistant pathogens. However, clinical use of aminoglycoside antibiotics is compromised by bacterial biofilm formation at subinhibitory concentrations or adverse side effects such as ototoxicity and nephrotoxicity at high antibiotic doses. Preparation of aminoglycoside formulation that allows on-demand drug delivery is a solution to this sticky issue. Here, we designed a new type of aminoglycoside hydrogels by cross-linking oxidized polysaccharides such as dextran , carboxymethyl cellulose , alginate , and chondroitin using aminoglycosides as cross-linkers. The hydrogel modulus, degradation rate and release kinetics can be precisely modulated by tailoring the aminoglycoside dose during gel formation. The aminoglycoside hydrogel showed fast gelation , self-healing and on-demand drug release behaviors, and high antibacterial activities in vitro and in vivo against both Gram-positive and Gram-negative bacteria. This study provides a facile and promising strategy to develop smart hydrogels for topical administration of aminoglycoside antibiotics.
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FOOD HYDROCOLLOIDS
·
2024
·
IF 11
Zhepeng Zhang,Yang Zhang,Chen Wang,Xianjin Liu,Hesham R. El-Seedi,Paula L. Gómez,Stella M. Alzamora,Xiaobo Zou,Zhiming Guo
Natural biopolymers have extensively applications in intelligent food packaging due to their renewable nature, easy availability, environmental-friendly, low-cost and excellent processibility. Recently, the integration or embedding of nanocomposites on polymer substrates to improve the multi-faceted performance of packaging materials has attracted widespread attention. Herein, the irregular spherical Co-based metal-organic framework (Co-MOF) loaded with phenol red (PR) and bromothymol blue (BTB) were fabricated, resulting in absorbed nanocomposites Co-MOF/PR and Co-MOF/BTB. Afterward, the nanocomposites were integrated into a sodium carboxymethyl cellulose (CMC-Na) substrate to prepare a CO 2 -responsive packaging material (CMC-Na/PR/Co-MOF and CMC-Na/BTB/Co-MOF) for real-time and in-situ monitoring apple freshness. The synthesis of Co-MOF and the hydrogen-bonding interactions between MOF and pigments adsorption were confirmed. The advantages exhibited by the film with adding Co-MOF, including excellent hydrophobicity and water resistance, improved time-temperature stability and mechanical properties, were explored. Notably, the Co-MOF-based composite film exhibited enhanced UV–visible barrier, anti-migration performance of pigment, and thermal stability. Finally, a significant linear response (R 2 = 0.9923 and 0.9781) between the total color difference values (ΔE) of Co-MOF-based film and hardness and total soluble solids (TSS) of fresh-cut Fuji apples at 4 °C was demonstrated, respectively. These findings indicate the significant potential of CMC-Na-based composite film containing multifunctional MOF nano-filler as a visual monitoring system, which will pave the way for fresh-cut fruit intelligent packaging.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Rongli Li,Tongjun Yang,Mingtao Ding,Lihua Zhang,Haibo Xie
Gold nanoparticles (AuNPs) with ultra-small size anchored onto support materials is highly desired towards good catalytic performance. In this study, aldehyde-functionalized chitin nanocrystals (ChNCs-PVMA) are prepared by surface-initiated electron transfer atom transfer radical polymerization (SI-ARGET ATRP) with vanillin methacrylate (VMA) as a functional monomer, which are used as reductant, stabilizer and support for the fabrication of AuNPs through an environmentally friendly process that eliminates the need for any additional reducing agents. The abundant aldehyde groups of the prepared ChNCs-PVMA are crucial to achieve ultra-small AuNPs with average size of 5.3 nm. The obtained Au@ChNCs-PVMA nanohybrid catalysts were systematically characterized by FTIR, XPS, XRD and TEM. Finally, the catalytic activity of the Au@ChNCs-PVMA catalysts are investigated for reduction of 4-NP and discoloration of azo/non-azo dyes, demonstrating excellent catalytic performance and reusability. These findings provide significant insights into the development of bio-supported nanohybrid catalyst for various environmental catalysis.
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AICHE JOURNAL
·
2024
·Vol. 70
·期 4
·e18366
·
IF 3.5
Meng-Meng Du,Bao-Chang Sun,Bei-Bei Wang,Yong Luo,Liang-Liang Zhang,Guang-Wen Chu,Hai-Kui Zou,Jian-Feng Chen
In this work, the kinetics study on the reaction between CO 2 and tertiary amine catalyzed by zinc(II)-1,4,8,11-tetraazacyclotetradecane complexes (CM) and zinc(II)-1,4,7,10-tetraazacyclododecane complexes (CN) was carried out in a stopped-flow device. The effects of the catalyst concentration, type of tertiary amines, and temperature on the reaction rate ( ν ) and catalytic activity ( φ ) were studied. It was found that the catalyst concentration, tertiary amine with higher p K a , and temperature had positive effects on ν . ν in N -methyl diethanolamine solution with 10.0 mol m −3 CM and CN were 16.62 and 26.05 folds than the uncatalyzed ν at 298 K, respectively. φ increased with increasing catalyst concentration, decreasing temperature and tertiary amine's p K a . In addition, the kinetics behavior of tertiary amine-CM/CN-CO 2 systems conformed to the Michaelis–Menten model. The activation energies in catalytic systems were 4%–15% lower than that in the non-catalytic systems.
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CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Xiaxia Yang,Linyan Yao,Xiuxia Sun,Lili Wang,Jianxi Xiao
Digital Light Processing (DLP) bioprinting has revolutionized tissue engineering and regenerative medicine with its speed and precision. Although commonly used in DLP printing, Gelatin Methacrylate (GelMA) lacks the mechanical strength and complex biological signaling of collagen, owing to the absence of collagen’s unique triple helix structure. However, collagen faces challenges such as denaturation at high temperatures and aggregation at neutral pH, limiting its use as a bioink. Herein, we present for the first time the development of a low-temperature DLP 3D printing technique using low-concentration Collagen Methacryloyl (ColMA) for fabricating durable and bioactive personalized scaffolds. Our study shows that pH significantly influences ColMA printing, with pH 3 producing low-concentration scaffolds that exhibit enhanced mechanical properties, minimal swelling, and prolonged resistance to enzymatic hydrolysis compared to high-concentration GelMA scaffolds, while low-concentration GelMA cannot be printed independently. ColMA scaffolds notably boost the proliferation, adhesion, migration, and differentiation of human foreskin fibroblasts. In vivo rat model tests demonstrate that ColMA scaffolds reduce inflammation, enhance collagen deposition, and accelerate epidermal regeneration. This method offers a robust solution for low-temperature DLP 3D printing of collagen ink, facilitating the creation of personalized scaffolds with superior bioactivity and representing a significant advancement in tissue engineering applications.
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