JOURNAL OF FLUORESCENCE
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2026
·
IF 3.4
Gu Jianlong,Gao Li,Cai Jieyun,Liu Yuchen,Meng Hongming,Long Jie,Ma Jing,Yang Bing,Wang Chunqiong,Yang Yaling
Carbon dots-based nanozymes show great potential for chemiluminescence (CL) detection due to their stability, excellent catalytic properties, good biocompatibility, and facile synthesis. In this study, bimetallic carbon dot nanoenzymes (Cur/Cu, Fe@CDs) that promote the chemiluminescence signal of luminol were synthesized. Cur/Cu, Fe@CDs possess high peroxidase (POD) activity and can catalyze the luminol/hydrogen peroxide reaction to generate a strong chemiluminescence intensity, which is approximately 8.98 times higher than the chemiluminescence spectral signal produced by the same concentration of K 3 [Fe(CN) 6 ]. Additionally, a sensitive and rapid method for detecting magnesium ions (Mg 2+ ) was developed by leveraging the enhancing effect of Mg 2+ on the CL signal in this system. The proposed CL technique for Mg 2+ demonstrated a linear response range from 3.33 to 166.67 µM and a detection limit of 0.99 µM. Moreover, the method was successfully applied to analyze Mg 2+ in cigarette paper samples through a spiking procedure, achieving a recovery rate of 91.1% to 109.0%. This research offers substantial evidence supporting the advancement of efficient and sensitive platforms for Mg 2+ CL detection.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Rongbin Nie,Huiyu He,Yiping Chen
Rapid and on-site detection of chloramphenicol (CAP) is of great significance for food safety. Herein, we established an affordable and portable homogeneous fiber optic chemiluminescent immunosensor (hoFOCI) by streamlining the immunoassay and integrating the detection platform. By immobilizing the artificial antigens of CAP and the luminescent functional groups onto the fiber optic, the biorecognition and signal conversion could be performed without any washing steps, which greatly reduced the time consumption and simplified the operation. For chemiluminescence signal detection, a low-cost and integrated detection platform was constructed, which was designed to be compatible with fiber optic probe . The hoFOCI had a good linear response to CAP in the concentration of 10–10 6 pg/mL, with a detection limit of 2.95 pg/mL. The feasibility was also validated by assaying CAP in real honey samples, and a high accuracy and reproducibility were obtained. Therefore, the proposed hoFOCI is expected to be appropriate for rapid and on-site detection of CAP in food stuffs.
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ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Zhichen Zhang,Yujing Zhu,Zejing Xing,Jing Li,Qin Xu,Jun-Jie Zhu,Cheng Ma
Electrochemiluminescence (ECL) has manifested a surface-confined emitting character and a low light background occurring near the electrode surface. However, the luminescence intensity and emitting layer are limited by the slow mass diffusion rate and electrode fouling in a stationary electrolyte. To address this problem, we developed an in situ strategy to flexibly regulate the ECL intensity and layer thickness by introducing an ultrasound (US) probe to the ECL detector and microscope. Herein, we explored the ECL responses and the thickness of ECL layer (TEL) under US irradiation in different ECL routes and systems. ECL microscopy with an ultrasonic probe discovered that ultrasonic radiation enhanced the ECL intensity under the catalytic route, while an opposite trend was observed under the oxidative-reduction route. Simulation results demonstrated that US promoted the direct electrochemical oxidation of TPrA radicals by the electrode rather than oxidant Ru(bpy)33+, which made the TEL thinner than that in the catalytic route under the same US condition. In situ US boosted the ECL signal from 1.2 times to 4.7 times by improving the mass transport and weakening electrode fouling due to the cavitation role. It significantly enhanced the ECL intensity beyond the diffusion-controlled ECL reaction rate. In addition, a synergistic sonochemical luminescence is validated in the luminol system to enhance the whole luminescence because cavitation bubbles of US promoted the generation of reactive oxygen species. This in situ US strategy provides a new opportunity to understand ECL mechanisms and a new tool in regulating TEL to meet the needs of ECL imaging.
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Advanced Healthcare Materials
·
2022
·Vol. 12
·期 8
·2202287
·
IF 11.092
Lei Guo,Zhaode Mu,Min Qing,Jing Zhou,Huizhen Li,Lulu Wang,Muyue Zhong,Lijuan Bai
Non-small cell lung cancer (NSCLC) is the most common type of lung cancer with substantial morbidity and mortality. Herein, a new signal-on electrochemiluminescence (ECL) immunosensor based on multiple amplification strategies is constructed for ultrasensitive detection of cytokeratin 19 fragment antigen 21-1 (CYFRA21-1) biomarker related to NSCLC. Polyethyleneimine (PEI) functionalized MXene is decorated with NiMn layer double hydroxide (NiMn LDH) to form MXene-PEI-NiMn LDH composite. Specially, the La-MOF@ZIF-67 bimetallic organic framework (named as LZBM) and MXene-PEI-NiMn LDH both served as coreaction accelerators to improve the ECL emission of the luminol-H 2 O 2 system. To be specific, Au nanoparticles (AuNPs) coated MXene-PEI-NiMn LDH is applied to immobilize primary CYFRA21-1 antibody (Ab 1 ), while AuNPs decorated LZBM was used for the loading of luminol and secondary CYFRA21-1 antibody (Ab 2 ) to form tracer label. Therefore, the ECL signal of the sandwich-type immunosensor is significantly enhanced due to the high loading capability for luminol and the synergistic catalytic ability for the decomposition of H 2 O 2 into reactive oxygen species (ROS). Under the optimal experimental conditions, the ECL immunosensor exhibited good analytical performances for CYFRA21-1 detection with a wide linear range (100 fg mL −1 −100 ng mL −1 ) and a low limit of detection (85.20 fg mL −1 ), providing a promising method for early diagnosis of NSCLC.
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ADVANCED FUNCTIONAL MATERIALS
·
2022
·Vol. 33
·期 8
·2212317
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IF 19.924
Juan Li,Jinhua Huang,Yongjian Jiang,Limin Wu,Yonghui Deng
Diverse diseases and increasing prevalence pose a serious threat to public health. Point-of-care testing (POCT) techniques have imposed superior requirements over sensitivity, selectivity, robustness, affordability, and high-throughput. However, transient signal, complex sample pretreatment, and low signal-to-noise ratio make POCT severely limited in detection accuracy, efficiency, and sensitivity. Here, an enzyme-assisted magnetic large-mesoporous nanoreactor (FS) is constructed for achieving persistent-chemiluminescence signal output and eliminating matrix interference in disease diagnosis. The core-shell structured FS synthesized via an interface coassembly method exhibits uniform size, large and open mesopores (≈22 nm), and intrinsic magnetic separability. Such unique FS acts as efficient nanoreactor for confined cascade reactions enable efficient persistent-chemiluminescence (pCL) signal transduction and high-SNR chromogenic analysis of diverse biomarkers. The developed pCL assays facilitate high-sensitive determination of chronic disease biomarkers glucose and uric acid with detection limits (DL) of 5.4 mg L −1 and 151.2 ng L −1 , respectively. The proposed chromogenic immunoassay enables an ultrasensitive and visual determination of alpha-fetoprotein with a DL of 1.2 ng L −1 , which is superior to previously published immunoassays. The feasibility of the developed methods for real-world applications are demonstrated in 159 clinical serum samples, and the determination results agree well with the clinical data. The proposed technique is expected to promote highly sensitive disease diagnosis in primary medical institutions and resource-limited areas since not relying on expensive automatic sampling and testing instruments. The good flexibility of the customizable nanoreactor makes it a powerful tool for developing various POCT techniques for rapid, sensitive, and accurate diseases diagnosis.
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ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Kaixiang Ji,Shiyu Xia,Xueqing Sang,Abdallah M. Zeid,Altaf Hussain,Jianping Li,Guobao Xu
FeOOH nanorods, as one-dimensional nanomaterials, have been widely used in many fields due to their stable properties, low cost, and easy synthesis, but their application in the field of chemiluminescence (CL) is rarely reported. In this work, FeOOH nanorods were synthesized by a simple and environmentally friendly one-pot hydrothermal method and used for the first time as a catalyst for generating strong CL with luminol without additional oxidant. Remarkably, luminol-FeOOH exhibits about 250 times stronger CL than the luminol-H2O2 system. Its CL intensity was significantly quenched by uric acid. We established a simple, rapid, sensitive, and selective CL method for the detection of uric acid with a linear range of 20–1000 nM and a detection limit of 6.3 nM (S/N = 3). In addition, we successfully applied this method to the detection of uric acid in human serum, and the standard recoveries were 95.6–106.4%.
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ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Jing Lei,Xiaoqing Sun,Yan Jin,Chunli Xu,Baoxin Li
Fe and Co single-atom catalysts (SACs) have been widely explored in many fields, while Zn SACs are still in their infancy stage. Herein, we unexpectedly found that atomically dispersed Zn2+ on N-doped carbon material (Zn-N-C) exhibited high catalytic activity on luminol-H2O2 chemiluminescence (CL) reaction. The Zn-N-C SACs were readily prepared through simple pyrolyzation of the cheap precursors (dopamine and ZnCl2). The mechanism of Zn SAC-catalyzed CL reaction of luminol-H2O2 was investigated in detail. The activity of Zn SACs originated from the Zn-N sites in the Zn-N-C structure. The monoatomic dispersion makes Zn2+ catalytic performance change from no activity to high activity in luminol-H2O2 CL reaction. This study demonstrated the particularity of the monatomic metal catalyst over the conventional metal ion. This work provides the unprecedented perspective for design of new metal SACs in CL reaction.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2023
·Vol. 34
·期 5
·1-9
·
IF 2.8
Chen Jiajia,Dai Fengting,Liu Haotian,He Zhengguang,Gao Hui,Liu Gen
In this work, three specialized oxidases for glucose, lactate and cholesterol are absorbed on carbon microspheres (CSs), which can produce hydrogen peroxide during their substrate-specific enzymatic activity. Electrochemiluminescent (ECL) is generated by the electrochemical reaction between luminol and hydrogen peroxide on an ECL biosensor array, whose light signals are collected by a CCD camera. Under optimum conditions, calibration curves are constructed for glucose, lactate, cholesterol concentrations and ECL intensities. The detection limits of the different biosensors are found to be 12 μM for glucose, 3 μM for lactate and 19 μM for cholesterol. Furthermore, the resulting ECL biosensor array is expected to be an alternative analytical tool in clinical analysis because of its good stability, acceptable precision and reproducibility.
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Acta Biomaterialia
·
2022
·
IF 10.633
Manman Liang,Limin Shang,Yixin Yu,Yujie Jiang,Qiang Bai,Junchi Ma,Dongqin Yang,Ning Sui,Zhiling Zhu
Chemodynamic therapy (CDT) employs Fenton catalysts to kill bacteria by converting hydrogen peroxide (H 2 O 2 ) into toxic hydroxyl radical (•OH). Among them, Fenton-type metal peroxide nanoparticles fascinate nanomaterials with intriguing physiochemical properties, but research on this antibacterial agent is still in its infancy. Herein, a distinct CuO 2 /TiO 2 heterostructure constituted of ultrasmall copper peroxide (CuO 2 ) nanoclusters and sonosensitized ultrathin oxygen vacancy-rich porous titanium oxide (OV-TiO 2 ) nanosheets was developed and was incorporated into microneedles for bilaterally augmented sono-chemodynamic and sonothermal antibacterial therapy. Engineering CuO 2 nanoclusters on the surface of TiO 2 nanosheets not only endows the Fenton catalytic activity for sono-chemodynamic therapy (SCDT), but also improves the sonodynamic and sonothermal performance of TiO 2 by narrowing the bandgap of TiO 2 and suppressing the recombination of electron–hole pairs. The high efficacy of this CuO 2 /TiO 2 integrated microneedle (CTMN) patch was systematically demonstrated both in vitro and in vivo with the eliminating rate >99.9999% against multidrug resistant (MDR) pathogens in 5 min as well as accelerated wound tissue healing. This work highlights a promisingly new and efficient strategy for the development of sonosensitive and chemoreactive nanomedicine for non-antibiotic therapies. Statement of significance Feton-type metal peroxides, a novel nanomaterial with self-supplied oxygen and hydrogen peroxide, can achieve effective antimicrobial activity in vitro. However, there is a lack of effective nanomaterial delivery systems and suitable means for in vivo activation/enhancement of antimicrobial activity during bacterial infected skin wound treatment. In this study, we designed and prepared efficient ultrasound activable microneedles that effectively addressed the deficiencies mentioned above and established a new paradigm for efficient utilization of metal peroxide nanomaterials and ultrasound based strategies. Noticeably, copper peroxide nanoclusters/oxygen vacancy-rich porous titanium oxide nanosheets (CuO 2 /TiO 2 ) integrated microneedle (CTMN) patch combines advantages of both sono-chemodynamic and sonothermal antibacterial therapy, achieving one of the most instant and effective antibacterial efficacy (>99.9999% in 5 min) in vivo reported till now.
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ACS Omega
·
2023
·
IF 4.1
Wei Wang,Han Li,Jiamin Lv,Ghulam Jilany Khan,Hong Duan,Juan Zhu,Nina Bao,Kefeng Zhai,Zhenglian Xue
Isodon suzhouensis from Suzhou, China, is a traditional Chinese herb with wide applications in medicine and food. The antioxidant activity against oxidative stress of the leaves of Isodon suzhouensis is a myth since long and is not explored earlier thoroughly. The present study is focused to explore the active components in Isodon suzhouensis leaf extracts responsible for antioxidant effects against oxidative stress and the potential mechanism of this activity. We obtained the chromatograms of Isodon suzhouensis leaf extracts by the high-performance liquid phase (HPLC) for possible detection of antioxidant constituents. Some compounds in Isodon suzhouensis leaf extracts were then further assessed through the luminol luminescence mechanism combined with HPLC analysis as well as with SwissTargetPrediction database that helped to screen out the other constituents. The targets for effects against oxidative stress were then further screened through the GeneCards database, and the PPI network was constructed. The targets were analyzed by GO and KEGG using the David database. The obtained results were then further studied by employing in vitro experimentation and protein expression analyses by Western blotting. It is found that Isodon suzhouensis leaf extracts contain rutin, isoquercetin, glaucocalyxin A, glaucocalyxin B, and other compounds with antioxidant activity. The activity map of the free radical scavenging signals from Isodon suzhouensis showed a strong ability to scavenge free radicals with the highest capacity of glaucocalyxin B followed by isoquercetin succeeding the glaucocalyxin A supervening the rutin. Further network pharmacological analyses and in vitro experimentation showed that Isodon suzhouensis leaf extracts interfere with TNF and the p38 MAPK signaling pathway for antioxidant effects against oxidative stress. Conclusively, it is found that Isodon suzhouensis leaf extracts possess strong antioxidant potential via targeting TNF and p38 MAPK signaling pathways against oxidative stress, providing scientific foundation for further studies on Isodon suzhouensis for the further therapeutic approach.
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MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 12
·1-10
·
IF 6.408
Sun Zhuo,Lu Juan,Zhang Xin,Shan Xiangyu,Wu Qian,Li Chao,Li Huiling,Yang Shuning,Tian Li
Electrospun nanofibers containing CdTe@ZnNi-metal–organic frameworks (CdTe@ZnNi-MOF NFs) were used for the first time in luminol-O 2 electrochemiluminescence (ECL) system to construct ECL sensor for chlorpyrifos (CPF) detection. Firstly, CdTe@ZnNi-MOF NFs obtained by blending method was used to modify the surface of a glassy carbon electrode, and then, the proposed solid-state ECL sensor was constructed by dropping luminol onto the surface of nanofiber benefiting from chitosan (CTS) viscosity. CdTe@ZnNi-MOF NFs with its large surface area and porosity can be used not only as luminol carrier but also as co-reaction promoter of the ECL system. The ECL sensor obtained satisfactory results in weakly alkaline solution under the synergistic action of CdTe@ZnNi-MOF NFs and luminol. The constructed ECL sensor can effectively detect CPF in the range 1.0 × 10 −14 –1.0 × 10 −9 M, and the detection limit was 6.23 × 10 −17 M (3 σ / m ). The constructed sensor was simple and sensitive, and can be used for the determination of CPF in vegetable samples. It not only broadened the application of MOFs in the field of ECL but also provided a new idea for expanding the application of the luminol-O 2 system. Graphical Abstract
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INTERNATIONAL JOURNAL OF HEAT AND MASS TRANSFER
·
2022
·
IF 5.431
Shuai Yuan,Mingyong Zhou,Xijiang Liu,Qiang Li,Bingyan Jiang
Microfluidic-based enrichment techniques have had a profound impact on chemistry, owing to their ability to improve the sensitivity of detection by increasing the concentration of analytes in minute fluid volumes. Herein, concentration enrichment of analyte solutes is achieved by using voltage assist in a microchip with two reservoirs. A numerical model is developed to describe the complex analyte focusing process, which to date has not been investigated before. Numerical computations are systematically conducted to study the influences of applied voltage, mass transfer coefficient, electrode location, reservoir diameter and number of mass transfer channels on the concentration enrichment in a designed Polydimethylsiloxane (PDMS)/Glass microfluidic electrophoresis chip. The electric fields, concentration distributions, and electrophoretic fluxes are obtained, which can reveal insightful enrichment mechanisms. It is found that the factors that can increase the electrophoretic flux in the mass transfer channel and enhance the electric field strength in the enrichment reservoir, such as moving the electrode back or increasing the number of mass transfer channels, are particularly important to improve the enrichment performance. The final microchip after considering the Joule heating effect has a 335-fold and 2.8-fold maximum and average concentration enrichments, respectively. Furthermore, this method is applied to the experimental enrichment of Rhodamine B and heavy metal cadmium (II) ions to test it performance.
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危险品化学品经营许可证（带存储）