Pharmaceuticals
·
2025
·Vol. 18
·期 12
·1795
·
IF 4.8
Baojie Du,Bingqing Zhang,Xiaofeng Ren,Jie Yang,Fan Yang,Chunyu Yan,Liping Li,Ruiping Zhang
Background/Objectives: Alkaline phosphatase (ALP) is a crucial enzyme in numerous pathological processes and a significant biomarker in clinical diagnostics. Conventional ALP detection methods are hampered by reliance on complex sample pretreatment, sophisticated instrumentation, time-consuming procedures, and high costs. This study aimed to develop a simple, rapid, and cost-effective colorimetric sensing method for ALP detection with enhanced resistance to matrix interference in biological samples.Methods: We designed a colorimetric assay based on bimetallic gold–platinum nanocatalysts (AuPt NPs) exhibiting peroxidase-like (POD-like) activity. The detection principle involves a dual-reaction cascade: (1) Alkaline phosphatase (ALP) catalyzes the conversion of trisodium L-ascorbic acid-2-phosphate (AA2P) into ascorbic acid (AA), and (2) the generated AA reduces oxidized 3,3′,5,5′-tetramethylbenzidine (oxTMB) produced by the catalytic activity of AuPt NPs. This method was evaluated for its detection performance in diluted human serum without complex sample pretreatment.Results: AuPt NPs exhibited resistance to biological matrix interference, enabling sensitive detection of ALP. The assay showed a linear ALP detection range of 0–90 mU·mL−1(R2= 0.994) and a limit of detection of 3.91 mU·mL−1. In spiked human serum, recoveries were 95.45–111.97%, with negligible interference from ions and biomolecules.Conclusions: We developed a simple, rapid, and reliable colorimetric sensor for ALP detection based on AuPt NPs. It overcomes limitations of conventional methods, holding great potential for clinical diagnostics and point-of-care applications.
研究领域:
纳米技术
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生物传感器
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临床化学
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酶检测
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纳米材料
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Wei Li,Shujing Zhou,Yihong Nan,Jiale Hu,Pei Luo,Yun Zhang,Ziyuan Li,Zhongsheng Yi,Jianmei Zou
Employing a facile and cost-effective room-temperature co-precipitation method, we synthesized a 2D bimetallic Co/Cu-MOF for the first time. • The 2D Co/Cu-MOF exhibits enhanced OXD-like activity, directly oxidizing TMB to produce blue oxTMB in the absence of H 2 O 2 . • A simple colorimetric sensing platform was developed for detecting AA and ALP, enabling their measurement in human serum. • The 2D Co/Cu-MOF exhibited significant antibacterial efficacy against both E. coli and S. aureus . Nanozymes have attracted extensive attention in sensing and antibacterial fields. Herein, a two-dimensional (2D) bimetallic Co/Cu-based metal-organic framework (Co/Cu-MOF) was successfully synthesized for the first time via a facile, economical one-step room-temperature co-precipitation method. The as-prepared Co/Cu-MOF exhibited excellent oxidase (OXD)-like activity, which could catalyze dissolved oxygen to generate reactive oxygen species (ROS) without H 2 O 2 , and further oxidize the 3,3′,5,5′-tetramethylbenzidine (TMB) to blue oxTMB. Based on the reduction of oxTMB by ascorbic acid (AA), a sensitive colorimetric sensing platform for AA was constructed, showing a linear range of 0.1–28 μM and a low detection limit of 0.091 μM (3σ/S). With AA as a mediator, this strategy was readily extended to alkaline phosphatase (ALP) detection, presenting a linear range of 0.2–6.5 U/L and a detection limit of 0.12 U/L. The proposed sensor was successfully applied to quantify AA and ALP in human serum samples. Furthermore, Co/Cu-MOF displayed strong antibacterial activity against both Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ), and its underlying antibacterial mechanism was elucidated. Collectively, this 2D bimetallic MOF provides a promising platform for biochemical sensing and antimicrobial technologies. Download: Download high-res image (142KB) Download: Download full-size image
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DOI
高影响因子
ACS Applied Nano Materials
·
2021
·
IF 5.097
Xiaobin Zhou,Mengjun Wang,Mengke Wang,Xingguang Su
Herein, a colorimetric detection strategy for alkaline phosphatase (ALP) activity was established based on an Fe-doped carbon-based nanozyme. By doping Fe into a Zn MOF and introducing dicyandiamide for pyrolysis, we obtained a two-dimensional carbon nanosheet with exceptional peroxidase-mimicking activity (Fe/C NS). The Fe/C NS has superior affinity to H2O2, can decompose H2O2 into reactive oxygen species, and further oxidize reducing substances such as phenol. ALP can hydrolyze disodium phenyl phosphate into phenol, while the Fe/C NS can promote the reaction between phenol and 4-aminoantipyrine in the presence of H2O2 to produce red quinone imine. Based on the changes of absorbance of the reaction system, ALP activity can be detected in a range of 0.05–6.00 U·L–1 with a detection limit of 0.03 U·L–1. Furthermore, we developed a quantitative relationship between ALP activity and color characteristic values by recording the RGB (red, green, and blue) values of color changes under different ALP concentrations, realizing the convenient determination of ALP activity without specific analytical equipment. This work can effectively broaden the application of nanozymes in biological analysis.
研究领域:
生物化学
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医学检验
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纳米生物学
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产品关联
DOI
高影响因子
International Journal of Nanomedicine
·
2025
·
IF 6.5
Yong Lian,Ning Ma,Qianying Cheng,Mingquan Luo,Zhen Xu,Fei He,Xiaomei Zhou,Ying Zhang,Dejun Jin,Yidan Kong,Yong Wang,Na Wei
Purpose Study aims to optimize the synthesis conditions for silver nanoparticle composites [ALP(D)-AgNPs] using a rapid and environmentally friendly method and investigate the antioxidant, antibacterial, and anticancer activities of the fabricated composite.Methods The polysaccharide component ALP-D was extracted and purified from the fruits of Amomum longiligulare and subsequently used for further experiments. The structure of ALP-D was characterized by FT-IR, monosaccharide composition and molecular weight.The optimal conditions for the green synthesis of silver nanoparticles using ALP-D were determined through single-factor experiments. The synthesized silver nanoparticles were characterized by UV-Vis spectroscopy, FT-IR, DLS, HRTEM and XRD. Finally, the in vitro antioxidant activity, antibacterial activity and anticancer activity of the nano-silver composite were evaluated.Results Single-factor experiments identified the optimal synthesis conditions for ALP(D)-AgNPs as a reaction time of 180 min, a temperature of 100 °C, and a 10:1 volume ratio of silver nitrate to ALP-D. The free radical scavenging activity of ALP(D)-AgNPs against DPPH and ABTS was significantly enhanced compared with that of ALP-D. The minimum inhibitory concentration (MIC) values of ALP(D)-AgNPs against E coli and B subtilis were 31.25 μg/mL, while the MIC value against S aureus was 62.5 μg/mL. The minimum bactericidal concentration (MBC) values of ALP(D)-AgNPs were 125 μg/mL for E coli, B subtilis, and S aureus. The IC50 values of ALP(D)-AgNPs on the MDA-MB-231, HepG2, Caco-2, and C6 cancer cell lines were 14.72 ± 0.23, 8.19 ± 0.65, 22.73 ± 3.01, and 15.77 ± 2.91 μg/mL, respectively.Conclusion In summary, we have identified a novel material for the green synthesis of silver nanoparticles. The results show the ALP(D)-AgNPs synthesized using the new material ALP-D exhibit excellent stability and dispersibility. Furthermore, the biological activity reveals that ALP(D)-AgNPs possess notable antioxidant, antibacterial, and cancer-suppressing activities.
研究领域:
材料科学
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生物化学
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药理学
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2019
·
IF 4.916
Nuanfei Zhu,Yuqi Zhu,Jin Wang,Eric Gyimah,Xialin Hu,Zhen Zhang
A novel indirect competitive fluorescence immunoassay based on the quenching of I - to silver clusters (AgNCs) was developed for the highly selective and ultrasensitive detection of sulfamethazine (SMZ). In this system, alkaline phosphatase (ALP) was labeled on the secondary antibody (Ab 2 ). And after a competition step, magnesium ascorbyl phosphate could be catalyzed to produce ascorbic acid under the catalysis of ALP. Subsequently, I 2 was introduced and further reduced to I - in the presence of ascorbic acid, triggering the fluorescence quenching of AgNCs dispersed in isopropanol (IPA) buffer. More importantly, trace I - could lead to an obvious reduction in fluorescence signal, indicating the sensitivity of this method would be greatly improved. Under the optimal condition, this improved method for the SMZ detection has a lower detection of limit (LOD, 0.05000 μg/L) with a wider range (0.1400–71.71 μg/L). After an evaluation, the fluorescence ELISA proposed in this work has satisfactory accuracy and reliability (recoveries, 84.18–118.6%; CV, 2.03–7.64%), illustrating good performance and great potential for the detection of trace SMZ in environmental and biological samples.
研究领域:
环境科学
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分析化学
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生物医学
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DOI
PubMed
高影响因子
MATERIALS LETTERS
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2016
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IF 2.437
Huan Zhou,Shiqin Kong,Zhiwei Liu,Yan Pan,Yang Liu,Mingzhi Luo,Linhong Deng
Calcium polyphosphate (CPP) incorporated calcium alginate (CA) composite beads were prepared via adding sodium polyphosphate (STPP) contained sodium alginate (SA) solution dropwise to calcium chloride (CaCl 2 ) solution. In the process Ca 2+ ion may attach to the -COO − in SA because of electrostatic attraction. Additionally, there is an interaction of PP to Ca 2+ to generate CPP, inducing intermolecular crosslinkages between CA and CPP. The rotational rheometer showed STPP addition had no significant impacts on the rheological properties of SA solution. While a combination of SEM, AFM, FTIR and swelling behavior study demonstrated CPP was successfully formed within the gel network of CA gel beads and played an active role in restricting the movability of the alginate polymer chains. The in vitro study implied CPP can promote the expression of ALP , Col-I, OCN and OPN of osteoblasts.
研究领域:
材料科学
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生物医学工程
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细胞生物学
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DOI
高影响因子
Analytical Methods
·
2019
·Vol. 11
·期 42
·5472-5477
·
IF 2.378
Linjie Wang,Kun Ye,Jianming Pan,Hongwei Song,Xin Li,Xiangheng Niu
As a typical biomarker for disease diagnosis in clinical practice, alkaline phosphatase (ALP) plays a critical role in regulating the dephosphorylation process of biomolecules. On this account, exploration of an effective and simple means of ALP activity measurement becomes very significant. In this work, we report a catalytic reaction-based colorimetric method for the monitoring of ALP activity via employing nanostructured MnO2 as a favorable oxidase mimic. The prepared MnO2 microspheres exhibit good oxidase-like capacity to induce the catalytic oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to deep-blue TMBox; when ALP hydrolyzes ascorbic acid phosphate, the produced ascorbic acid (AA) with a certain reducing power can destroy the MnO2 enzyme mimic by reducing it to Mn2+, dramatically suppressing the TMB chromogenic reaction. Based on the strategy, a convenient and high-performance colorimetric method was established for ALP activity sensing, giving a wide linear range from 0.5 to 120 U L−1. With the help of N-ethylmaleimide as an efficient masking agent for biothiols, the colorimetric approach could provide excellent specificity for ALP activity detection. Reliable and precise measurement of the analyte in practical samples was also verified by our assay, indicating its promise as a feasible tool for clinical diagnosis.
研究领域:
生物化学
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医学诊断
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纳米材料科学
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产品关联
DOI
高影响因子
Journal of Analysis and Testing
·
2019
·Vol. 3
·期 3
·228-237
·
IF 0
Niu Xiangheng,Ye Kun,Li Zhibo,Zhao Hongli,Wang Linjie,Pan Jianming,Song Hongwei,Lan Minbo
Developing reliable and facile approaches for alkaline phosphatase (ALP) sensing is important due to its role as a clinical biomarker for many diseases. In this study, we proposed a new and convenient colorimetric assay based on the pyrophosphate (PPi)-mediated oxidase-mimicking activity switching of nanosized MnFe 2 O 4 for the detection of ALP. The synthesized MnFe 2 O 4 exhibited high oxidase-like activity to catalyze the oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to its blue product TMBox in the presence of dissolved O 2 , leading to a color reaction rapidly and remarkably; PPi could significantly inhibit the activity of the MnFe 2 O 4 nanozyme via the strong interaction between PPi and the Fe(III) species in MnFe 2 O 4 , resulting in the suppression of the TMB color reaction; when ALP was added, it hydrolyzed the PPi substrate to phosphate (Pi) that had no obvious effect on the MnFe 2 O 4 activity, and such that the TMB color reaction catalyzed by the nanozyme could be observed again. With the above principle, linear colorimetric determination of ALP in the scope of 0.6–55 U L −1 was achieved, giving the limit of detection down to 0.27 U L −1 . Besides, the developed assay could provide selective response toward ALP against other co-existing biological species. Furthermore, reliable detection of ALP in human serum samples was verified by our assay, revealing its great promise as an effective and facile tool for ALP monitoring in clinical practice.
研究领域:
分析化学
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生物化学
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纳米技术
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医学诊断
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DOI
Food Bioscience
·
2025
·
IF 5.9
Haotian Zhao,Wenlong Sun,Wenfei Cai,Chang Liu,Wei Wang,Naiyan Lu
This study investigated the effects of Arctium lappa L. polysaccharides (ALP) on exercise performance and gut microbiota composition in mice subjected to acute sleep deprivation (SD) with a focus on the gut-muscle axis. The results revealed that ALP significantly enhanced the forelimb grip strength and muscle endurance in SD mice, thereby improving their overall exercise capacity. Additionally, ALP regulated the energy metabolism by increasing blood glucose, liver glycogen, and muscle glycogen levels, while simultaneously reducing oxidative stress and inflammation. Histological analysis revealed that ALP strengthened intestinal barrier function by promoting the expression of tight junction proteins ZO-1 and Occludin. In terms of gut microbiota, ALP increased the Alpha diversity of the gut microbiota and optimized its structure. Notably, ALP significantly elevated the abundance of beneficial bacteria such as Akkermansia and Muribaculaceae, while inhibiting the proliferation of harmful bacteria like Colidextribacter and Lachnoclostridium. These findings suggest that ALP improves the exercise capacity and overall health of SD mice by modulating the gut microbiota and intestinal barrier function, providing new insights into the use of dietary interventions to alleviate health issues caused by sleep deprivation.
研究领域:
营养学
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微生物学
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运动生理学
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DOI
高影响因子
TALANTA
·
2019
·
IF 4.916
Can Chen,Fenghua Geng,Yongxiang Wang,Haidong Yu,Lin Li,Sheng Yang,Jinhua Liu,Wei Huang
The present work constructs a sequentially triggered nanoswitch (STN) for sequential detection of Cr 3+ , P 2 O 7 4− (PPi) and alkaline phosphatase (ALP) depending on polythymine (T40) templated fluorescent Cu nanoparticles (Cu NPs). A significant phenomenon is that Cr 3+ can only causing 5% QE of fluorescent Cu NPs synthesized by lower than 500 μM Cu 2+ , but the fluorescence of the Cu NPs synthesized by more than 500 μM Cu 2+ can be quenched up to 90% QE by the same concentration of Cr 3+ . Then the quenched fluorescence of CuNP-Cr 3+ complex provides a sensing platform for PPi due to the strong binding between Cr 3+ and PPi, resulting in dissociation of Cr 3+ from the surface of Cu NPs and the recovery of fluorescence emission . Further ALP hydrolysis of PPi disrupts Cr 3+ -PPi assemble and Cr 3+ is released to interact with Cu NPs, which induces fluorescence quenching again. Thus, sequentially detection of Cr 3+ (LOD, 0.03 μM), PPi (LOD, 0.005 μM) and ALP (LOD, 0.125 mU/mL) was successfully implemented with high sensitivity and selectivity. The sensor is also successfully used for Cr 3+ , PPi and ALP assays in the human serum. Additionally, the sensitive “on-off-on-off” sensing behavior of the Cu NPs allow three chemical inputs (Cr 3+ , PPi and ALP) to construct a logic gate.
研究领域:
生物化学
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分析化学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
Journal of Stomatology Oral and Maxillofacial Surgery
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2022
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IF 2.48
Yi Sun,Jianlu Shi,Xiaoan Luo,Xuehong Xu
Objective Human periodontal ligament stem cells (hPDLSCs) refer to one kind of somatic stem cells that are capable of differentiating into multiple cell kinds and undergoing robust clonal self-renewal. This work was unearthed to elucidate the possible molecular mechanism of miR-142–3p in mediating osteogenic differentiation of hPDLSCs by targeting SGK1 . Methods The hPDLSCs were isolated, cultured, and identified. hPDLSCs were identified by immunofluorescence staining and multiple differentiation ability detection. Cell proliferation ability was assessed by CCK-8 assay. hPDLSCs were induced using osteogenic differentiation medium. ALP activity was detected by alkaline phosphatase (ALP) staining and ALP activity assay, and mineralized nodule formation was determined by alizarin red staining. The expression levels of osteogenic differentiation marker proteins ALP, RUNX2, and OCN were measured by RT-qPCR. miR-142–3p candidate targets were obtained through bioinformatics analysis. The relationship between miR-142–3p and SKG1 was verified. Results miR-142–3p in hPDLSCs after osteogenic induction was down-regulated. Elevated miR-142–3p restricted hPDLSCs proliferation, and diminished ALP activity and mineralized nodule formation, as well as the expression of ALP, RUNX2, and OCN, while miR-142–3p inhibition led to inverse results. miR-142-3p inhibited SKG1 expression. SKG1 overexpression promoted hPDLSC proliferation and osteogenic differentiation, and reversed the inhibitory function of miR-142–3p on hPDLSCs. Conclusion This study highlights that miR-142–3p represses osteogenic differentiation of hPDLSCs by reducing SGK1 expression.
研究领域:
干细胞生物学
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分子生物学
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口腔生物学
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2021
·Vol. 9
·期 13
·2998-3004
·
IF 6.331
Jia An,Yongqin Hu,Guoyi Liu,Meizhu Chen,Rubing Chen,Ying Lyu,Mengdi Yuan,Mengfei Luo,Yufei Liu
Considering the limited sensitivity and accuracy of single-signal assay strategies, the multi-signal assay strategy has sparked significant excitement in recent years. In this study, for the first time, we reported a one-pot method in situ synthesis of carbon-containing nanoparticles (CNPs) via p-aminophenol (AP) and diethylenetriamine (DETA). The CNP solution exhibits yellow and light blue fluorescence under UV-light. Moreover, the CNPs exhibited excellent photoluminescence stability even under extreme conditions. Inspired by the alkaline phosphatase (ALP)-triggered specific catalytic reaction, we constructed an ultrasensitive fluorescence and colorimetric two-channel strategy for monitoring the ALP activity. By optimizing the detection parameters, the detection limits for both fluorometric and colorimetric were 0.05 mU mL−1. Moreover, the strategy showed high specificity and was successfully applied to monitor the ALP activity level in human serum samples. The analytical strategy opened a new window for the detection of the ALP activity, screening of the ALP inhibitor, and disease diagnosis.
研究领域:
分析化学
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生物传感器
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纳米技术
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医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）