ACS Applied Bio Materials
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2025
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IF 4.7
Xinyu Gao,Xinjiani Chen,Suxian Song,Changle Lu,Zhen Zhang,Yu Zhou,Liping Yao,Xiaojun Liu,Rongqing Zhang
Bletilla striata polysaccharide (BSP) is effective at healing wounds and has important application value in the research and development of biomedical materials. In this study, BSP, chitosan, and sodium β-glycerophosphate were used to generate a complex hydrogel with a very small pore size of 10–30 μm without the use of cross-linking agents. By improving the cross-linking density, the mechanical property defects caused by excessive BSP dissolution were overcome without affecting its physiological activity. Moreover, the small molecule Danshen sodium (SDSS) was loaded into the three-dimensional network of this hydrogel to form a hydrogel drug carrier system. SDSS could be released in the long term, and the total amount released after 53 h was 96.26 ± 2.57%. The BSP hydrogel had good water absorption (169.47 ± 4.54%) and bonding properties. In vitro studies confirmed that it has a good antibacterial performance and biocompatibility and the ability to promote cell proliferation (>200%) and migration. Molecular experiments confirmed that the hydrogel promotes collagen expression. In vivo experiments using a mouse wound healing model confirmed that the hydrogel has an excellent ability to promote wound healing, particularly during the first 7 days of the wound. The wound healing rate of the hydrogel group was higher than that of the blank group by 27.61% (p < 0.05), and the effect of the hydrogel to promote wound healing was confirmed by wound tissue staining.
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高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2025
·
IF 5.2
Cong Wang,Wenting Liu,Yong Huang,Lin Zheng,Lili Wang,Ruixi Li,Chang Yang
Risedronate sodium (RIS) is a primary treatment for postmenopausal osteoporosis, but oral administration requires patients to remain upright to prevent side effects like esophagitis, gastritis, and ulcers, which can be difficult for those with fatigue or bone pain. This study developed RIS-loaded Bletilla striata polysaccharide microneedles (RIS-BMNs) with sufficient mechanical strength for effective skin penetration and drug delivery. Franz diffusion cell experiments showed that RIS-BMNs achieved 3 times higher in vitro transdermal absorption than the RIS solution and 2.6 times more than RIS patches. The stimulation test found that RIS-BMNs caused minimal skin irritation, which resolved within 12 h. RIS-BMNs also promoted cell proliferation and wound healing, as shown by in vitro cell viability and migration tests, and improved bone trabeculae and density in osteoporotic rats, according to micro-CT imaging and H&E staining. Biochemical markers (BGP, IL-6, CTX-I, TRAP, OPG, and PINP) indicated that RIS-BMNs had anti-osteoporotic effects comparable to oral RIS but without gastrointestinal side effects, as confirmed by H&E staining. Our new transdermal method for RIS could improve adherence in treating postmenopausal osteoporosis.
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JOURNAL OF BIOMATERIALS APPLICATIONS
·
2023
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IF 2.9
Kai Zhao,Zhengbo Hu,Mingyuan Zhou,Yuchi Chen,Fangmei Zhou,Zhishan Ding,Bingqi Zhu
Diabetic wounds impose enormous distress and financial burden on patients, and finding effective dressings to manage wounds is critical. As a Chinese herbal medicine with a long history of Clinical application, Bletilla striata has significant medicinal effects in the therapy of various wounds. In this study, PLA and the pharmacodynamic substances of Bletilla striata were prepared into fibrous scaffolds by emulsion electrospinning technology for the management of diabetic wounds in mice. The results of scanning electron microscopy showed that the core-shell structure fibre was successfully obtained by emulsion electrospinning. The fibre membrane exhibited excellent water absorption capability and water vapor transmission rate, could inhibit the growth of Staphylococcus aureus and Pseudomonas aeruginosa, had good compatibility, and achieved excellent healing effect on diabetic wounds. Especially in the in vivo wound healing experiment, the wound healing rate of composite fibre membrane treatment reached 98.587 ± 2.149% in 16 days. This work demonstrated the good therapeutic effect of the developed fibrous membrane to diabetic wound, and this membrane could be potentially applied to chronic wound healing.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Yingbei Huang,Fulin Shi,Liming Wang,Yu Yang,Bilal Muhammad Khan,Kit-Leong Cheong,Yang Liu
This study aims at developing an effective, safe and economic hydrogel, with wound healing ability, by making use of polysaccharides. Bletilla striata polysaccharide (Bsp) and the bioactive natural polymers, carboxymethyl chitosan (CMC), were physically blended with Carbomer 940 (CBM 940 ) in order to promote gel-forming and improve water retention. FT-IR displayed hydrogen bonding between CBM 940 and CMC played a role in physical gel formation. XRD showed broad and weak intensity peak of Bsp/CMC/CBM 940 hydrogel. SEM, rheological measurement and water loss test revealed that the best hydrogel with optimum characteristics in relation to porous structure, elastic property and water retention could be obtained by mixing Bsp, CMC and CBM 940 in a ratio of 4:1:1. In vitro studies proved that the Bsp/CMC/CBM 940 hydrogel possessed blood compatibility. M293T cells viability was over 85% via MTT assay which revealed non-cytotoxicity. Hydroxyl radical scavenging property highly improved while Bsp combined with CMC. The full-thickness wound experiment illustrated 71.64 ± 6.64% and 83.80 ± 5.56% wound healing rates for Bsp4:CMC1:CBM 940 1 hydrogel on Day 7 and Day 14, respectively, which showed no significant differences (p > 0.05) in comparison to the positive control. Histological observation expressed epithelization, dense collagen fiber and neovascular formation in hydrogel group on Day 14. Bsp/CMC/CBM 940 hydrogel can, hence, serve as an attractive candidate for healing wounds.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2026
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IF 15
Qiantao Zhang,Lai Chai,Hui Liu,Xueer Hu,Yujing Niu,Hongli Cao,Xin Rao,Mingyuan Zhou,Yuchi Chen,Xiaoqing Ye,Fangmei Zhou,Zhishan Ding,Bingqi Zhu
Ulcerative colitis (UC) is an immune-mediated chronic inflammatory bowel disease that severely impairs patients' quality of life. Efficient oral colon-targeted delivery systems are urgently needed to improve local therapeutic efficacy while minimizing systemic exposure. Herein, we developed a pH-responsive Eudragit S100-coated coacervate microdroplet system for the oral delivery of natural Bletilla striata polysaccharide (BSP), termed BSP@EU-Coac. The optimized BSP@EU-Coac microdroplets exhibited a spherical morphology with an average hydrodynamic diameter of 3.86 ± 0.82 μm, an encapsulation efficiency of 85.03 ± 3.66%, and a drug loading capacity of 9.29 ± 0.93%. In vitro release studies showed that BSP@EU-Coac effectively limited premature BSP release under simulated gastric and small intestinal conditions, while achieving pH-triggered sustained release in simulated colonic medium, with a cumulative release of approximately 88.25% within 96 h. In vitro assays further demonstrated that BSP@EU-Coac showed good cytocompatibility at the working concentration and markedly reduced intracellular ROS levels, with ROS fluorescence intensity decreased by 53.95% and 51.13% in RAW264.7 macrophages and Caco-2 cells, respectively. After oral administration, fluorescence imaging confirmed that BSP@EU-Coac preferentially accumulated in the inflamed colon and maintained detectable colonic retention for up to 24 h. In a DSS-induced colitis mouse model, BSP@EU-Coac significantly alleviated UC symptoms, as evidenced by improved body weight recovery, reduced disease activity index, and restoration of colon length from 4.99 ± 1.23 cm in the model group to 8.66 ± 1.92 cm. Mechanistically, BSP@EU-Coac modulated macrophage polarization by reducing the M1-like CD86⁺CD206⁻ population from 29.26% to 8.95% and increasing the M2-like CD86⁻CD206⁺ population to 20.70%, accompanied by suppressed pro-inflammatory cytokine expression, enhanced tight junction protein expression, reduced oxidative stress, and partial restoration of gut microbiota homeostasis. Overall, this study demonstrates that BSP@EU-Coac is a promising oral colon-targeted polysaccharide delivery platform for UC therapy through integrated regulation of oxidative stress, immune response, epithelial barrier repair, and gut microbiota.
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2026
·
IF 4
Gejie Shi,Kunmei Li,Nan Yang,Derong Yang,Sanhua Li,Jiarui Ding,Haiyan Tuo,Haowei Liu,Yun Liu,Feng Pan
BACKGROUND Although tuber polysaccharides of Bletilla striata have been extensively studied, the leaf polysaccharides, which constitute over 25% of the plant's biomass, are frequently discarded as agricultural waste. Given the well-documented bioactivities of plant polysaccharides, this represents significant untapped potential. Current cardioprotective agents exhibit limitations against doxorubicin-induced cardiotoxicity, creating demand for novel natural alternatives. This study thus aimed to isolate and characterize the primary polysaccharide from B. striata leaves and evaluate its antioxidant capacity and cardioprotective effects, thereby converting this agricultural waste into a therapeutic agent. RESULTS The purified homogeneous polysaccharide BSP-L2-1 (Mw > 500 kDa) was structurally characterized as a highly branched heteropolysaccharide featuring triple-helix conformation, with a mannose:galacturonic acid:glucose:galactose:arabinose molar ratio of 6.02:1.74:2.70:10.00:4.32. In vitro assays demonstrated potent antioxidant activity, with BSP-L2-1 achieving 72.37% inhibition of ABTS radicals at a concentration of 6 mg mL −1 . More notably, in a doxorubicin-injured H9c2(2-1) cardiomyocytes model, BSP-L2-1 exhibited significant cardioprotection by suppressing reactive oxygen species and malondialdehyde overproduction, enhancing glutathione and superoxide dismutase activity and concurrently preserving mitochondrial function. Additionally, toxicity assessments in a zebrafish model confirmed no observable adverse effects across a broad concentration range (100–900 μg mL −1 ). CONCLUSION BSP-L2-1, a novel acidic natural polysaccharide isolated from underutilized B. striata leaves, mitigated doxorubicin-induced cardiotoxicity by attenuating oxidative stress and preserving mitochondrial function. These findings support its development as a natural adjuvant to prevent doxorubicin-induced cardiotoxicity, with potential applications ranging from functional foods to pharmaceutical formulations. © 2026 Society of Chemical Industry.
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JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
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2025
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IF 4.9
Zhaolei Peng,Yulin Wang,Kai Liu,Chunyan Cai,Dejun Yang,Jingjing Xi,Mingyi Shi,Xiaofang Li
In this work, Bletilla striata polysaccharide-modified SeNPs (BSPs-SeNPs) was prepared to address the instability of SeNPs as a result of its outstanding performance in the field of natural polymer materials. BSPs-SeNPs were synthesized using Na 2 SeO 3 as the selenium source and ascorbic acid as the reducing agent. The FTIR, XRD and EDS results demonstrated the successful synthesis of BSPs-SeNPs, which exhibited excellent nanoparticle characteristics, with a particle size of 66.9 ± 1.30 nm and Se concentration of 12.19 ± 0.62 %. Stability tests indicated that BSPs-SeNPs maintain structural integrity under various storage conditions, pH levels, temperatures, and ionic strengths. The mice acute liver injury (ALI) model was created with 300 mg/kg APAP, BSPs-SeNPs decreased serum ALT and AST values as expected. And the restored SOD, GSH and MDA values showed correlation with the in vitro antioxidant activity assay results (DPPH · , ABTS · , · O 2 − , and · OH). In general, BSPs-SeNPs is successfully synthesized, which enlightened the conjunction between natural polysaccharide and inorganic nanoparticles, but also encouraged the extensive use of BSPs in the field of pharmaceutical excipients.
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2023
·Vol. 24
·期 22
·16286
·
IF 5.6
Zhengbo Hu,Kai Zhao,Xingcan Chen,Mingyuan Zhou,Yuchi Chen,Xiaoqing Ye,Fangmei Zhou,Zhishan Ding,Bingqi Zhu
The healing process of a diabetic wound (DW) is often impeded by a series of interrelated factors, including severe infection, persistent inflammation, and excessive oxidative stress. Therefore, it is particularly crucial to develop a medical dressing that can address these issues simultaneously. To this end, different ratios ofBletilla striatapolysaccharide (BSP) and berberine (BER) were physically blended with Carbomer 940 (CBM940) to develop a composite hydrogel as a medical dressing. The BSP/BER hydrogel was characterized using SEM, FTIR, rheological testing and other techniques. The anti-inflammatory, antioxidant, and antibacterial properties of the hydrogel were evaluated using cell and bacterial models in vitro. A DW model of ICR mice was established to evaluate the effect of the hydrogel on DW healing in vivo. The hydrogel exhibited excellent biocompatibility and remarkable antibacterial, anti-inflammatory, and antioxidant properties. In addition, animal experiments showed that the BSP/BER hydrogel significantly accelerated wound healing in DW mice. Among the different formulations, the LBSP/BER hydrogel (2% BSP, mBER:mBSP= 1:40) demonstrated the most remarkable efficacy. In conclusion, the BSP/BER hydrogel developed exhibited immense properties and great potential as a medical dressing for the repair of DW, addressing a crucial need in clinical practice.Keywords:diabetic wound;Bletilla striatapolysaccharide;berberine;hydrogel
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Yujia Wu,Wen Zhang,Jianyu Huang,Zichen Luo,Junsong Li,Lingchong Wang,Liuqing Di
As polysaccharide from Bletilla striata (BSP) was anticipated with mucoadhesive improvement in sodium alginate (SA) microspheres, BSP was mixed with SA to construct a composite microsphere to retain in the gastrointestinal tract for a long time. The morphological properties, particle size and thermodynamic properties of the microspheres in combination with comprehensive evaluations in the swelling properties, mucin adsorption, ex vivo and in vivo gastric retention were determined to characterize the mucoadhesion of SA-BSP blend microspheres. Results showed that the prepared microspheres were discrete and spherical. The addition of BSP increased flexibility and reduced rigidity of SA microsphere. Furthermore, the swelling property, mucin adsorption ability and the retention rate on the gastric mucosa of SA matrix were increased after blending with BSP. Mucoadhesion tests showed the SA-BSP microspheres stayed much longer in rats' stomach than the SA microsphere did. Above all, the SA-BSP microspheres with the enhanced mucoadhesion suggested being a potential drug carrier in developing the gastroretentive drug delivery system.
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PROCESS BIOCHEMISTRY
·
2014
·
IF 2.524
Jian-Wei Dong,Le Cai,Jie Xiong,Xiu-Hua Chen,Wei-Ying Wang,Ni Shen,Bei-Lei Liu,Zhong-Tao Ding
The tubers of Bletilla striata were fermented with Fusarium avenaceum and Fusarium oxysporum . The total phenolic contents (TPCs) and antioxidant and antibacterial activities of the extracts from the fermented products were measured. Additionally, the extracts of both F. avenaceum and F. oxysporum fermented B. striata (FBS) were characterized by UV–vis spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy. The results showed that the F. avenaceum and F. oxysporum FBSs possessed higher TPCs and exhibited stronger antioxidant and antibacterial activities ( Bacillus subtilis ) in comparison with original material not co-fermented with microorganisms. Chemical characterization of the two extracts with UV–vis, FT-IR, and 1 H NMR implied that some of the phenolic glycosides may be deglycosylated and that bibenzyl compounds and saccharides may be metabolized in both FBS systems. Microbial fermentation enhances the TPC as well as the antioxidant and antibacterial activities of B. striata . The present study suggests that F. avenaceum and F. oxysporum fermentations were effective in B. striata processing.
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Frontiers in Immunology
·
2024
·
IF 5.7
Yue Zhongqun,Zhu Yue,Chen Teng,Feng Tingting,Zhou Ying,Zhang Jiaojiao,Zhang Ning,Yang Jing,Luo Gang,Wang Zuhua
Background: Vitamin E, which is also known as tocopherol, is a compound with a polyphenol structure. Its esterified derivative, Vitamin E succinate (VES), exhibits unique anticancer and healthcare functions as well as immunomodulatory effects. Natural polysaccharides are proved to be a promising material for nano-drug delivery systems, which show excellent biodegradability and biocompatibility. In this study, we employed a novel bletilla striata polysaccharide-vitamin E succinate polymer (BSP-VES) micelles to enhance the tumor targeting and anti-colon cancer effect of andrographolide (AG).Methods: BSP-VES polymer was synthesized through esterification and its structure was confirmed using 1H NMR. AG@BSP-VES was prepared via the dialysis method and the drug loading, entrapment efficiency, stability, and safety were assessed. Furthermore, the tumor targeting ability of AG@BSP-VES was evaluated through targeted cell uptake and in vivo imaging. The antitumor activity of AG@BSP-VES was measured in vitro using MTT assay, Live&Dead cell staining, and cell scratch test.Results: In this study, we successfully loaded AG into BSP-VES micelles (AG@BSP-VES), which exhibited good stability, biosafety and sustained release effect. In addition, AG@BSP-VES also showed excellent internalization capability into CT26 cells compared with NCM460 cells in vitro. Meanwhile, the specific delivery of AG@BSP-VES micelles into subcutaneous and in-situ colon tumors was observed compared with normal colon tissues in vivo during the whole experiment process (1–24 h). What’s more, AG@BSP-VES micelles exhibited significant antitumor activities than BSP-VES micelles and free AG.Conclusion: The study provides a meaningful new idea and method for application in drug delivery system and targeted treatment of colon cancer based on natural polysaccharides.
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Carbohydrate Polymer Technologies and Applications
·
2024
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IF 6.2
Xue Han,Hai Liu,Zhiqin Song,Lin Yang,Xiao Liu,Jingxia Zhang,Menglan Gan,Jiawei Liu,Yeshan Zhang,Mingkai Wu
Bletilla striata polysaccharide (BSP) is an extract derived from the rare and valuable Chinese herb Bletilla striata [Thunb.] Reichb. f.. Although BSP research has progressed considerably, producing portable BSP dressings with hemostatic efficacy remains challenging. This study describes a novel dressing with a reticulate structure. To create this structure, BSP sponges were cross-linked with chitosan and lyophilized. The resulting BSP sponges had excellent mechanical properties, a high density of pores, and an outstanding capacity for fluid absorption. Both in vitro and in vivo experiments demonstrated that the BSP sponges had excellent hemostatic, antibacterial, and anti-inflammatory properties; they also exhibited good stimulation of cell growth and wound healing properties. Furthermore, they were less likely to provoke complications than traditional materials. Cytocompatibility assays revealed that they also stimulated cell proliferation. Therefore, our study suggests that BSP sponges have promising clinical applications as wound dressings.
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