Analytical Methods
·
2017
·Vol. 9
·期 46
·6507-6512
·
IF 1.9
Fang Li,Ruihua Zhang,Huaibin Kang,Yonggang Hu,Yunhong Liu,Junya Zhu
Clenbuterol (CLB) is a β-agonist drug that is illegally added as a growth-promoting agent in livestock. In this study, a facile and sensitive method for the detection of CLB using a personal glucose meter (PGM) was reported. Clenbuterol–bovine serum albumin functionalized magnetic beads were used to compete with CLB for binding to its corresponding antibody and gold nanoparticle–invertase–secondary antibody conjugates. In the sucrose solution, the invertase catalyzes the hydrolysis of sucrose to produce a large amount of glucose and quantitative readout using the PGM. The ratio of signals from the PGM before and after addition of CLB exhibits a good linear correlation with the logarithm of the CLB concentrations in the range of 0.1–100 ng mL−1 with a detection limit of 0.1 ng mL−1 (S/N = 3). Finally, the proposed method was successfully applied for the detection of CLB residues in pork and liver samples.
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2017
·
IF 4.529
Yiwei Tang,Jingwen Gao,Xiuying Liu,Xue Gao,Tao Ma,Xiaonan Lu,Jianrong Li
As an ideal biomimetic antibody, molecularly imprinted polymer (MIP) has shown great promise in immunoassays. Here, we developed a covalent imprinting method to prepare MIP artificial antibody towards clenbuterol on the well surface of a microtiter plate. Template molecule (clenbuterol) was splited by hydrolysis with functional monomer and removed by methanol/acetic acid solution, and then three-dimensional cavities were fabricated in the MIP, which can rebind template molecule via hydrogen bond interaction. Using this artificial antibody, we developed a novel biomimetic enzyme-linked immunosorbent assay (ELISA) with excellent sensitivity and specificity. The linear range for clenbuterol was 10 −5 to 1000 μg L −1 , with a detection limit of 10 −7 μg L −1 . Cross reactivity of this MIP artificial antibody to other four structural analogs was less than 0.93%. This method was applied to determine clenbuterol in real samples with satisfactory result, suggesting a promising application of the biomimetic ELISA in food and environmental analysis.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Lu Yang,Tong Zhai,Jing Wang,Hong Zheng,Jing-Min Liu,Shuo Wang
To achieve high throughput detection of clenbuterol while reducing matrix interference, we developed a simple and integrated nano-inspection platform. Herein, based on the simplicity and convenience of paper chip, a multi-layer paper chip-chemiluminescence (CL) sensor was designed in the form of “48-well plate” type. The all-in-one paper chip-CL sensor has integrated the covalent organic frameworks (COFs) layer for sample matrix purification, high affinity nanobody layer for specific capture of target, and CL rapid response layer for the final determination. The signal readout was realized by the inhibitory effect of clenbuterol on the K 3 [Fe(CN) 6 ] CL system. Detection of clenbuterol in pork meat was verified by measuring the CL intensity and satisfactory recoveries (85.9 %–97.3 %) were achieved. 48 samples could be simultaneously detected on one-chip in one-test, favoring the high-throughput detection. Especially, the work explored a new pathway for design of portable analytical instruments to achieve immediate on-site detection of hazards.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Scientific Reports
·
2017
·Vol. 7
·期 1
·1-9
·
IF 4.259
Wang Jingyun,Zhang Lei,Huang Youju,Dandapat Anirban,Dai Liwei,Zhang Ganggang,Lu Xuefei,Zhang Jiawei,Lai Weihua,Chen Tao
The probe materials play a significant role in improving the detection efficiency and sensitivity of lateral-flow immunochromatographic test strip (ICTS). Unlike conventional ICTS assay usually uses single-component, solid gold nanoparticles as labeled probes, in our present study, a bimetallic, hollow Au-Ag nanoparticles (NPs) labeled ICTS was successfully developed for the detection of clenbuterol (CLE). The hollow Au-Ag NPs with different Au/Ag mole ratio and tunable size were synthesized by varying the volume ratio of [HAuCl4]:[Ag NPs] via the galvanic replacement reaction. The surface of hollow Ag-Au NPs was functionalized with 11-mercaptoundecanoic acid (MUA) for further covalently bonded with anti-CLE monoclonal antibody. Overall size of the Au-Ag NPs, size of the holes within individual NPs and also Au/Ag mole ratio have been systematically optimized to amplify both the visual inspection signals and the quantitative data. The sensitivity of optimized hollow Au-Ag NPs probes has been achieved even as low as 2 ppb in a short time (within 15 min), which is superior over the detection performance of conventional test strip using Au NPs. The optimized hollow Au-Ag NPs labeled test strip can be used as an ideal candidate for the rapid screening of CLE in food samples.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Food Measurement and Characterization
·
2023
·Vol. 17
·期 5
·4627-4637
·
IF 3.4
Hu Jun,Zhan Chaohui,Xu Zhen,Shi Hongyang,Wang Hao,He Yong,Ouyang Aiguo
The abuse of clenbuterol hydrochloride (CL) is a serious threat to people’s life and health. The traditional THz detection method can only detect the sample at the milligram level, which is not suitable for trace analysis of the sample. In this paper, a THz metamaterial enhanced micro-nano structure was designed based on the electromagnetic theory to realize the highly sensitive detection of the trace CL by THz spectroscopy. Firstly, four pretreatment methods were used to correct the spectrum, including AirPLS, AsLS, Backcor and BEADS. Secondly, four algorithms, namely, PCA, CARS, UVE, and SPA were used to extract features from the spectra. Finally, the PLS and LS-SVM models are established. The AirPLS-CARS-LS-SVM model has the best result, with the R p of 0.9743, the RMSEP of 2.90 × 10 –5 , and the LOD of 9.67 × 10 –5 mg/mL. This study will facilitate the rapid, nondestructive and highly sensitive detection of the trace CL in food. Besides, this study has significant implications for promoting the detection of other types of clenbuterol and trace substances. Graphical abstract
✓ 已验证
DOI
高影响因子
MEDICINAL CHEMISTRY RESEARCH
·
2017
·Vol. 27
·期 3
·944-953
·
IF 1.277
Wang Zechun,Han Xinxin,Wang Ning,Wang Ruiyong,Chang Junbiao
Clenbuterol (CLB) is a notorious “lean meat essence”, which has harmful effects on human health. In this study, the binding of CLB to two proteins, human serum albumin (HSA) and the human fat mass and obesity associated protein (FTO), have been investigated by spectroscopic and molecular modeling methods. Fluorescence data revealed that CLB could bind to HSA (or FTO) to form a complex. Thermodynamic analysis and molecular modeling indicated that the binding process was a spontaneous molecular interaction procedure, in which hydrophobic interaction played a major role. Results indicated that CLB could lead to some microenvironmental changes of both proteins.
✓ 已验证
DOI
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Yanli Zhou,Huanqing Zhang,Zhu Chang,Baoxian Ye,Maotian Xu
A highly sensitive electrochemical sensor for the simultaneous determination of clenbuterol (CLB) and salbutamol (SAL) was fabricated by the modification of graphene-Nafion (GN-Nafion) composite film on a glassy carbon electrode (GCE). Both CLB and SAL caused a pair of redox peaks at the GN-Nafion/GCE in 0.04 M Britton-Robinson buffer solution. Obvious anodic peak currents for the oxidation of both CLB and SAL were observed under the optimal condition. The difference of oxidation peak potentials for CLB and SAL was about 200 mV, which made it possible for simultaneous determination of these compounds using the GN-Nafion/GCE. The detection limit for CLB and SAL was 0.13 μM and 0.11 μM (S/N=3), respectively. The proposed method was applied to simultaneously detect CLB and SAL in pork meat with satisfactory results. Otherwise, the excellent reproducibility, stability and selectivity were obtained at the proposed electrode.
✓ 已验证
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Nina Zhao,Xue Cheng,Yu Li,Shuaishuai Wei,Zhouping Wang
Clenbuterol hydrochloride (CLB) is prohibited for use in food-producing animals due to its associated health risks, necessitating the urgent development of facile, highly sensitive on-site detection methods. In this study, a truncated aptamer (C40, Kd = 90.29 nM) with high affinity for CLB was obtained via rational truncation guided by molecular docking. Based on this aptamer, a novel colorimetric aptasensor was constructed, operating on the principle of regulating the peroxidase-mimetic activity of Pd@CeO 2 NPs nanozymes through the aptamer. After the C40 aptamer was adsorbed onto the surface of Pd@CeO 2 NPs via electrostatic interactions, its catalytic activity was significantly suppressed. In the presence of CLB, the specific binding between the aptamer and CLB triggered the dissociation of the aptamer from the nanozyme surface, thereby restoring the catalytic activity and generating a strong colorimetric signal in the TMB-H 2 O 2 chromogenic system. Under the optimized conditions, the aptasensor exhibited a wide linear detection range for CLB from 0.01 to 1000 ng/mL, with a limit of detection as low as 8.2 pg/mL, and demonstrated high specificity against structural analogs and other common interferents. In spiked pork samples, the recovery rates of the developed aptasensor ranged from 98.7% to 101%, with relative standard deviations below 4.69%, outperforming commercial ELISA kits. The strategy of “rational truncation of aptamers for regulating nanozyme activity” provided a new avenue for developing high-performance biosensors targeting small molecules.
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Yuechun Li,Han Zhang,Zhaowen Cui,Sijie Liu,Jingke Xu,Conghui Jia,Yaqian Chen,Lulu Wang,Jing Sun,Daohong Zhang,Mingqiang Zhu,Jianlong Wang
Exploring a novel strategy for strengthening the catalytic activity of enzyme facilitates the development of a sensitive enzyme-linked immunosorbent assay (ELISA). Herein, a chemical staining (CS) strategy was firstly discovered to possess the ability to directly improve the catalytic activity of horseradish peroxidase. Based on this discovery, coomassie brilliant blue was introduced into ELISA to establish a CS enhanced ELISA (CS-ELISA) to detect clenbuterol (CL) by simply staining monoclonal antibodies. Satisfactorily, the most important analytical parameters of CS-ELISA, including sensitivity (0.074 ng mL −1 ) and linear range (0.2–2 ng mL −1 ) were all improving 2-folds compared with conventional ELISA. Moreover, the CS-ELISA shows good applicability in the detection of CL in pork tenderloin samples. The proposed CS-ELISA shows various advantages, such as cost-effective, easily accessible, enhanced catalytic activity of enzyme, higher sensitivity, and broader linear range, providing a new insight into enhanced ELISA for food safety.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2015
·
IF 6.409
Yiwei Tang,Ziyuan Gao,Shuo Wang,Xue Gao,Jingwen Gao,Yong Ma,Xiuying Liu,Jianrong Li
A novel fluorescence probe based on upconversion particles, YF 3 :Yb 3+ , Er 3+ , coating with molecularly imprinted polymers (MIPs@UCPs) has been synthesized for selective recognition of the analyte clenbuterol (CLB), which was characterized by scan electron microscope and X-ray powder diffraction. The fluorescence of the MIPs@UCPs probe is quenched specifically by CLB, and the effect is much stronger than the NIPs@UCPs (non-imprinting polymers, NIPs). Good linear correlation was obtained for CLB over the concentration range of 5.0–100.0 μg L −1 with a detection limit of 0.12 μg L −1 ( S/N =3). The developed method was also used in the determination of CLB in water and pork samples, and the recoveries ranged from 81.66% to 102.46% were obtained with relative standard deviation of 2.96–4.98% ( n =3). The present study provides a new and general tactics to synthesize MIPs@UCPs fluorescence probe with highly selective recognition ability to the CLB and is desirable for application widely in the near future.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Zhejiang University-SCIENCE B
·
2016
·
IF 1.303
Sovichea Lay,Hai-ning Yu,Bao-xiang Hu,Sheng-rong Shen
A pre-treatment methodology for clenbuterol hydrochloride (CLEN) isolation and enrichment in a complex matrix environment was developed through exploiting molecularly imprinted polymers (MIPs). CLEN-imprinted polymers were synthesized by the combined use of ally-β-cyclodextrin (ally-β-CD) and methacrylic acid (MAA), allyl-β-CD and acrylonitrile (AN), and allyl-β-CD and methyl methacrylate (MMA) as the binary functional monomers. MAA-linked allyl-β-CD MIPs (M-MAA) were characterized by Fourier transform-infrared (FT-IR) spectroscopy and a scanning electron microscope (SEM). Based upon the results, M-MAA polymers generally proved to be an excellent selective extraction compared to its references: AN-linked allyl-β-CD MIPs (M-AN) and MMA-linked allyl-β-CD MIPs (M-MMA). M-MAA polymers were eventually chosen to run through a molecularly imprinted solid-phase extraction (MISPE) micro-column to enrich CLEN residues spiked in pig livers. A high recovery was achieved, ranging from 91.03% to 96.76% with relative standard deviation (RSD) ≤4.45%.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 4
·1-10
·
IF 5.705
Ji Renyue,Chen Shuai,Xu Wei,Qin Zhen,Qiu Jing Fu,Li Chao Rui
An ultrasensitive immunosensor for the direct detection of the illegally used livestock feed clebuterol (CLB) is described. It is based on the use of a glassy carbon electrode modified with an MoS 2 -AuPt nanocomposite and on biotin-streptavidin interaction. The use of MoS 2 -AuPt accelerates electron transfer, and this leads to a sharp increase in the electrochemical signal for the electrochemical probe hydrogen peroxide. Differential pulse voltammetry was used to record the current signal at a peak potential of −0.18 V (vs SCE). Under optimal conditions, the electrode has a linear response in the 10 pg·mL −1 to 100 ng·mL −1 CLB concentration range and a 6.9 pg·mL −1 detection limit (based on the 3σ criterium). This immunosensor is sensitive, highly specific and acceptably reproducible, and thus represents a valuable tool for the determination of CLB in pork.
Schematic of a voltammetric immunosensor for the determination of clenbuterol (CLB) based on the use of a nanocomposite prepared from molybdenum disulfide and a gold-platinum alloy (MoS 2 -AuPt), and making use of the biotin-streptavidin system.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）