JOURNAL OF FLUORESCENCE
·
2026
·
IF 3.4
Gu Jianlong,Gao Li,Cai Jieyun,Liu Yuchen,Meng Hongming,Long Jie,Ma Jing,Yang Bing,Wang Chunqiong,Yang Yaling
Carbon dots-based nanozymes show great potential for chemiluminescence (CL) detection due to their stability, excellent catalytic properties, good biocompatibility, and facile synthesis. In this study, bimetallic carbon dot nanoenzymes (Cur/Cu, Fe@CDs) that promote the chemiluminescence signal of luminol were synthesized. Cur/Cu, Fe@CDs possess high peroxidase (POD) activity and can catalyze the luminol/hydrogen peroxide reaction to generate a strong chemiluminescence intensity, which is approximately 8.98 times higher than the chemiluminescence spectral signal produced by the same concentration of K 3 [Fe(CN) 6 ]. Additionally, a sensitive and rapid method for detecting magnesium ions (Mg 2+ ) was developed by leveraging the enhancing effect of Mg 2+ on the CL signal in this system. The proposed CL technique for Mg 2+ demonstrated a linear response range from 3.33 to 166.67 µM and a detection limit of 0.99 µM. Moreover, the method was successfully applied to analyze Mg 2+ in cigarette paper samples through a spiking procedure, achieving a recovery rate of 91.1% to 109.0%. This research offers substantial evidence supporting the advancement of efficient and sensitive platforms for Mg 2+ CL detection.
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DOI
PubMed
高影响因子
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2026
·
IF 5.2
Fanfan Zhang,Xuemei Hu,Yue Zhang,Baiyuan Sha,Fengshi Shang,Wenjie Wang,Rui Liu,Hao Wang
Hepatocellular carcinoma (HCC) poses a severe threat to public health, with clinical therapy limited by narrow surgical indications, chemotherapeutic side effects, and targeted drug resistance. Herein, we developed Fe 3+ coordination Polydopamine@Poly (lactic-co-glycolic acid)-Curcumin (Fe 3+ -PDA@PLGA-Cur, FePPC) nanoparticles integrating photothermal therapy (PTT), chemodynamic therapy (CDT), sonodynamic therapy (SDT), and curcumin (Cur)'s intrinsic anticancer activity for synergistic HCC treatment. FePPC was fabricated via emulsion solvent evaporation, in-situ dopamine polymerization, and Fe 3+ coordination chelation, with its structure and physicochemical properties characterized by FT-IR, UV-vis, SEM, XPS, and particle size analysis. FePPC exhibited uniform spherical morphology (198.58 nm), favorable stability, 73.60 ± 0.51% encapsulation efficiency, and NIR-triggered temperature-dependent Cur release. Under 808 nm NIR light (2 W/cm 2 , 4 cm), FePPC achieved a 24.4 °C temperature elevation with robust photothermal stability. Catalytic assays confirmed FePPC generated ·OH via Fe 3+ -mediated Fenton-like reactions, 1 O 2 under NIR irradiation, and ·O₂⁻ upon ultrasound stimulation, realizing synergistic reactive oxygen species (ROS) production. In vitro experiments on HepG2 cells showed time-dependent FePPC internalization; the FePPC + PTT + CDT + SDT group reduced cell viability to 53.45%, induced 47.55% apoptosis, elevated intracellular ROS, disrupted mitochondrial membrane potential, downregulated LDH, modulated Bax/Bcl-2 expression, and inhibited cell migration. This study highlights FePPC's potential as a multifunctional nanoplatform for HCC therapy via coordinated chemical-biological interactions.
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DOI
高影响因子
Pharmaceutics
·
2025
·Vol. 17
·期 2
·177
·
IF 5.5
Jun Li,Dongqing Huang,Wanchen Liao,Yulin Wang,Yibiao Liu,Ping Luan
Background: The excessive accumulation of Aβ plays a critical role in the development of Alzheimer’s disease. However, the therapeutic potential of drugs like curcumin is often limited by low biocompatibility and BBB permeability. In this study, we developed a nanomaterial, BP-PEG-Tar@Cur, which was designed to enhance the biocompatibility of (curcumin) Cur, target Aβ, and augment BBB permeability through near-infrared (NIR) photothermal effects.Methods: Soluble Aβ, ThT fluorescence, and Aβ depolymerization fluorescence experiments were conducted to evaluate the ability of BP-PEG-Tar@Cur to inhibit Aβ aggregation and dissociate Aβ fibrils. Cell uptake assays were performed to confirm the targeting ability of BP-PEG-Tar@Cur towards Aβ. In vitro mitochondrial ROS clearance and in vivo detection of inflammatory factors were used to assess the anti-inflammatory and antioxidant properties of the nanodrug. Water maze behavioral experiments were conducted to evaluate the effect of BP-PEG-Tar@Cur on spatial memory, learning ability, and behavioral disorders in AD mice.Results: The nanodrug effectively inhibited Aβ aggregation and dissociated Aβ fibrils in vitro. BP-PEG-Tar@Cur demonstrated efficiency in curbing ROS overproduction in mitochondria and dampening the activation of microglia and astrocytes triggered by Aβ aggregation. Water maze behavioral experiments revealed that BP-PEG-Tar@Cur enhanced spatial memory, learning ability, and alleviated behavioral disorders in AD mice.Conclusions: Collectively, these findings demonstrate that BP-PEG-Tar@Cur has the potential to be an effective targeted drug for inhibiting Aβ aggregation and improving cognitive impairment in AD mice.
研究领域:
神经科学
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药理学
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纳米医学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Linqian Hou,Qiulan Li,Jitao Li,Dezhi Yang,Yaling Yang
A curcumin based iron, copper and boron doping carbon dots (Cu,Fe,B/Cur-CDs) with ascorbic acid oxidase (AAO)- and peroxidase (POD)-like activities were synthesized via microwave digestion. The Cu,Fe,B/Cur-CDs oxidize ascorbic acid (AA) to dehydroascorbic acid (DHAA), which reacts with o -phenylenediamine (OPD) to generate fluorescent 3-(1,2-dihydroxyethyl) furo [3,4-b]quinoxaline-1-one (DFQ). Simultaneously, it catalyzes OPD/H₂O₂ to fluorescent 2,3-diaminophenazine (DAP) via POD-like activity. In the presence of metronidazole (MNZ), it induces competitive inhibition by competing with OPD for the active sites of Cu, Fe, B/Cur-CDs. This reduces the generation of •OH and •O₂ − radicals, hindering the oxidation of OPD to form DAP and thus decreasing the system's fluorescence intensity. Additionally, MNZ quenches the fluorescence of DFQ at 420 nm via the inner filter effect. Therefore, a dual-signal fluorescence sensor for MNZ detection was constructed. The sensor achieved 91.6–109.9 % and 86.2–116.2 % recovery in food samples using standard addition, demonstrating high sensitivity for dual-signal detection systems.
研究领域:
分析化学
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纳米技术
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生物化学
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环境科学
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食品安全
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Ya-li Xiang,De-yu Tang,Ling-ling Yan,Luo-lin Deng,Xing-han Wang,Xin-yang Liu,Qing-han Zhou
The challenge of drug resistance in bacteria caused by the over use of biotics is increasing during the therapy process, which has attracted great attentions of the clinicians and scientists around the world. Recently, photodynamic therapy (PDT) triggered by photosensitizer (PS) has become a promising treatment method because of its high efficacy, easy operation, and low side effect. Herein, the poly- l -lysine (PLL) modified metal-organic framework (MOF) nanoparticles, ZIF/PLL-CIP/CUR, were synthesized to allow both reactive oxygen species (ROS) responsive drug release and photodynamic effect for synergistic therapy against drug resistant bacterial infections. The PLL was modified on the shell of the zeolite imidazole framework (ZIF) by the ROS-responsive thioketal linker for controllable CIP release. CUR were encapsulated in ZIF as the photosensitizer for blue light mediated photodynamic effect to produce singlet oxygen ( 1 O 2 ) and superoxide anion radical ( O 2 − ) for efficient inhibition towards methicillin-resistant Staphylococcus aureus ( MRSA ). The charge conversion from negative charge (−4.6 mV) to positive charge (2.6 mV) was observed at pH 7.4 and pH 5.5, and 70.9 % CIP was found released at pH 5.5 in the presence of H 2 O 2 , which suggests the good biosafety at physiological pH and ROS-responsive drug release of the as-prepared nanoparticle in the bacterial microenvironment. The as-prepared nanoparticles could effectively kill MRSA and disrupt bacterial biofilm by combination of chemo- and photodynamic therapy. In mice model, the as-prepared nanoparticles exhibited excellent biosafety and synergistic effect with 98.81 % healing rate in treatment of MRSA infection, which is considered as a promising candidate in combating drug resistant bacterial infection.
研究领域:
纳米技术
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药学
·
生物医学工程
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微生物学
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治疗学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2018
·
IF 4.946
Xinyu Sun,Xiaoban Guo,Mingyu Ji,Jiulin Wu,Wenjin Zhu,Jianhua Wang,Cui Cheng,Li Chen,Qiqing Zhang
This study investigated the effects of a novel edible coating combining fish gelatin with curcumin/β-cyclodextrin (CUR/βCD) emulsion on the quality of grass carp fillets (GCFs) during storage at 4 °C. For all samples, the quality parameters, including weight loss, pH, total volatile basic nitrogen (TVB-N), peroxide value (PV), thiobarbituric acid (TBA) value, SDS-PAGE, free amino acids (FAA), microbiological (total viable counts (TVC), Pseudomonas counts, yeasts and molds, and H 2 S-producing bacteria), color and sensorial characteristics, were tested periodically. The coatings containing CUR/βCD emulsions exhibited better preservative effects than gelatin/βCD coating. Therefore, fish gelatin coating enriched with CUR/βCD emulsion can be used as an effective way to maintain the quality of GCF and extend its shelf life during storage at 4 °C.
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PubMed
高影响因子
Applied Materials Today
·
2022
·
IF 8.663
Xu Zhang,Ye He,Xuan Li,Chuanchuan Lin,Zhang Yuan,Liangliang Dai,Feng Ma,Yi Lv,Tony Jun Huang,Mudan Ren,Kaiyong Cai,Shuixiang He
Endoscopic submucosal dissection (ESD) is one of the most important strategies for early gastrointestinal tumor treatment. However, the ideal healing of artificial ulcers produced after ESD procedure are still hard to achieve. In a gastric ulcer environment, the inherent peristole, acid condition and excessive oxidative stress seriously affect the therapeutic outcome of traditional medication. Curcumin (Cur) is a promising anti-inflammatory drug for gastric ulcer treatment, but its long-term use is still challenging. A polymer-assisted drug delivery system in gastrointestinal disease treatment is a new direction but is still at an initial stage. Herein, we report a hybrid hydrogel consisting of catechol motif-modified methacrylated gelatin (GelMAc) and methacrylated poly (vinyl alcohol) (PVAMA). GelMAc brought significant adhesive property and biocompatibility. PVAMA could effectively provide stability and mechanical strength. Moreover, mesoporous polydopamine nanoparticles were prepared as the drug carrier to load Cur via π-π stacking and hydrogen bonding interactions, and they were encapsulated in the hybrid GelMAc/PVAMA hydrogel. The prepared sample was proved to have a unique stability on gastric tissue even bearing acid (pH 2) and oxidative stress (H 2 O 2 ) conditions. The slowly released Cur from the hydrogel was capable of inducing M2 polarization of macrophages. In an in vivo gastric ulcer model, the prepared sample remarkably improved gastric ulcer healing by curbing the pro-inflammatory response and alleviating oxidative stress. An accelerated reepithelization and angiogenesis were also observed. Thus, this study provides a promising hydrogel for the treatment of ESD-induced ulcers, which may attract more attentions to explore the deeper combination between biomaterials and gastrointestinal research.
研究领域:
生物医学工程
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药剂学
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胃肠病学
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材料科学
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DOI
高影响因子
ACS Omega
·
2024
·
IF 3.7
Yiping Weng,Xiuchen Yuan,Shijie Fan,Weihao Duan,Yadong Tan,Ruikai Zhou,Jingbin Wu,Yifei Shen,Zhonghua Zhang,Hua Xu
The treatment of various large bone defects has remained a challenge for orthopedic surgeons for a long time. Recent research indicates that curculigoside (CUR) extracted from the curculigo plant exerts a positive influence on bone formation, contributing to fracture healing. In this study, we employed emulsification/solvent evaporation techniques to successfully fabricate poly(ε-caprolactone) nanoparticles loaded with curculigoside (CUR@PM). Subsequently, using three-dimensional (3D) printing technology, we successfully developed a bioinspired composite scaffold named HA/GEL/SA/CUR@PM (HGSC), chemically cross-linked with calcium chloride, to ensure scaffold stability. Further characterization of the scaffold’s physical and chemical properties revealed uniform pore size, good hydrophilicity, and appropriate mechanical properties while achieving sustained drug release for up to 12 days. In vitro experiments demonstrated the nontoxicity, good biocompatibility, and cell proliferative properties of HGSC. Through alkaline phosphatase (ALP) staining, Alizarin Red S (ARS) staining, cell migration assays, tube formation assays, and detection of angiogenic and osteogenic gene proteins, we confirmed the HGSC composite scaffold’s significant angiogenic and osteoinductive capabilities. Eight weeks postimplantation in rat cranial defects, Micro-computed tomography (CT) and histological observations revealed pronounced angiogenesis and new bone growth in areas treated with the HGSC composite scaffold. These findings underscore the scaffold’s exceptional angiogenic and osteogenic properties, providing a solid theoretical basis for clinical bone repair and demonstrating its potential in promoting vascularization and bone regeneration.
研究领域:
生物医学工程
·
组织工程
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骨科
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药学
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材料科学
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DOI
PubMed
高影响因子
Current Drug Delivery
·
2023
·Vol. 20
·期 9
·1391-1403
·
IF 2.4
Yu Linjin,Chao Chuyan,Li Qinglan,Ye Songling,Lin Jiasen,Zhong Shuling,Xuan Qiancheng,Xu Kailin,Zhao Suqing
Background: Coenzyme Q10 (Q10) is a powerful lipophilic antioxidant with poor solubility in aqueous media. Curcumin (Cur) is a natural polyphenolic phytochemical molecule with poor aqueous solubility. The liposome is an improved administration of drugs because it is biocompatible and
permeable for nutraceutical delivery. Chitosan, a hydrophilic polymer, is often used as a polymer coating for its good biocompatible and biodegradable properties, and its relatively low toxicity level. Methods: Q10 and Cur co-loaded liposomes coated with chitosan (Q10-Cur-Lip-Chi) were
constructed. The co-encapsulation of Q10 and Cur in liposomes coated with chitosan was verified by TEM, DLS, DSC, FT-IR, and XRPD. The release profile and antioxidant activity of Q10-Cur-Lip-Chi were accessed. Results: The particle size of Q10-Cur-Lip-Chi was about 1440 nm with narrow
particle distribution. A satisfactory encapsulation efficiency (EE) of Q10 was about 98%, and 25% for that of Cur. Q10-Cur- Lip-Chi showed higher solubility and better pH resistance with 98.5% of Q10 and Cur retention at pH 7.0 - 9.0. Q10-Cur-Lip also showed great salt stability with a vesicle
size change of less than 5%. PSof Q10-Cur-Lip-Chi changed less than 10% at 4°C of storage. Q10-Cur-Lip-Chi also exhibited a good controlled release profile with its accumulative release of less than 34% for Q10 and 30% for curcumin after 24 h. The Q10-Cur-Lip-Chi performed a synergistic
effect on antioxidant activity reaching 41.86±1.84%, which was 5.9 times higher than that of Q10, 2.5 times higher than that of Cur, and 1.7 times higher than that of the mixture. Conclusion: The co-encapsulation Q10-Cur-Lip-Chi improves the solubility and stability of Q10 and
Cur for good release performance and antioxidative activity.
研究领域:
药剂学
·
纳米技术
·
药物递送系统
·
生物物理学
·
药理学
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DOI
PubMed
高影响因子
BIOMATERIALS
·
2022
·
IF 15.304
Yu-Xiang Ge,Tai-Wei Zhang,Lei Zhou,Wang Ding,Hai-Feng Liang,Zhi-Chao Hu,Qin Chen,Jian Dong,Feng-Feng Xue,Xiao-Fan Yin,Li-Bo Jiang
Poor immunogenicity and compromised T cell infiltration impede the application of immune-checkpoint blockade (ICB) immunotherapy for osteosarcoma (OS). Although autophagy is involved in enhancing the immune response, the synergistic role of autophagy in ICB immunotherapy and the accurate control of autophagy levels in OS remain elusive and challenging. Here, we designed a pH-sensitive autophagy-controlling nanocarrier, [email protected] @PEG-FA (CBZP), loading a natural derivative, curcumin (CUR), to boost the immunotherapeutic response of PD-1/PD-L1 blockade by activating immunogenic cell death (ICD) via autophagic cell death, and BMS1166 to inhibit the PD-1/PD-L1 interaction simultaneously, enhancing the tumor immunogenicity and sensitizing the antitumor T cell immunity. After entering tumor cells, the pH-sensitive nanoparticles induced autophagy and decreased the intracellular pH , which in turn further facilitated the release of CUR to enhance autophagic activity. Transferring CBZP to orthotopic OS tumor-bearing mice showed powerful antitumor effects and established long-term immunity against tumor recurrence, accompanied by enhanced dendritic cell maturation and tumor infiltration of CD8 + T lymphocytes. Collectively, CBZP exhibited synergistic effects in treating OS by combining ICD induction with checkpoint blockade, thereby shedding light on the use of autophagy control as a potential clinical therapy for OS.
研究领域:
肿瘤学
·
免疫疗法
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纳米技术
·
药理学
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DOI
PubMed
高影响因子
Signal Transduction and Targeted Therapy
·
2025
·Vol. 10
·期 1
·1-18
·
IF 52.7
Yue Yan,Li Shuoyuan,Su Qiang,Wan Xufeng,Li Qiaochu,Zhang Zhuang,Xu Hong,Zheng Fuyuan,Zhang Yangming,Tong Le,Cao Jian,Zhao Long,Chen Xiaoting,Li Qi,Zeng Yi,Wang Haoyang,Deng Yi,Zhou Zongke,Wang Duan
Conventional antibiotic strategies often fail to consistently suppress escaping planktonic bacteria and even induce antibiotic resistance, allowing implant-associated infections (IAIs) to persist. In this study, we demonstrated an antibiotic-free coating engineered with an herbal bioheterojunction featuring a shell-in-shell structure where Cu 2 O forms the core, strontium (Sr) is loaded in the inner shell, and curcumin (Cur) is nucleated in situ at the outer heterointerface (Cu 2 O-Sr/Cur). Ultrasound-triggered reactive oxygen species (ROS) generation by the outer heterostructure (Cu 2 O/Cur), coupled with Cu(I)-induced cuproptosis-like bacterial death, achieved antibacterial rates of 99.56% against S. aureus and 99.43% against E. coli . When ultrasonication ceases, the released Cu(I) undergoes disproportionation reactions to form Cu(II), which can chelate with Cur to form Cu-Cur metal complexes. These complexes exhibit enhanced antioxidative properties through self-catalytic regulation, the scavenging of ROS, and the activation of anti-inflammatory M2 macrophage phenotype. Moreover, strontium release from the inner shell simultaneously suppressed osteoclast activity and promoted osteogenesis, resulting in trabecular number and thickness increases of 129.03% and 56.71%, respectively, compared with those in control group. Therefore, our work establishes a sequential treatment strategy for the antibacterial properties and osteointegration ability of IAIs.
研究领域:
生物医学工程
·
抗菌材料
·
纳米医学
·
生物材料
·
感染控制
·
骨组织工程
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产品关联
DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2024
·
IF 10
Xuan Qin,Haoliang Shi,Zhen Wen,Binbin Chu,Hongyang Li,Houyu Wang,Yao He,Xuhui Sun
Electrotherapy is of great interest in the field of tissue repair as an effective, well-tolerated, and noninvasive treatment. Triboelectric nanogenerator (TENG) has shown advantages in promoting wound healing due to its peak output characteristic and low Joule heating effect. However, it is limited in infected wound healing due to poor antimicrobial capacity. Here, a wearable triboelectric stimulator (WTS) is developed that consists of a flexible TENG (F-TENG) and a triboelectric-responsive drug delivery hydrogel (TR-DDH) for healing of bacterium-infected wounds. F-TENG can generate pulsed current to wounds by converting mechanical energy from body movements. Polypyrrole is prone to reduction and volume contraction under electrical stimulation, resulting in desorption of curcumin nanoparticles (CUR NPs) from the polypyrrole in TR-DDH. Therefore, the highly efficient and controllable release of CUR NPs can be achieved by triboelectric stimulation. According to the in vitro and in vivo experiments, WTS has the greatest antimicrobial effect and the fastest promotion of infected wound healing compared to treatment with electrical stimulation or curcumin. Finally, the safety assessment demonstrates that the WTS has excellent tissue safety for chronic wound healing. Synergistic therapy with WTS provides an efficient strategy for chronic wound healing and smart-responsive drug delivery systems.
研究领域:
生物医学工程
·
纳米技术
·
伤口愈合
·
药物递送系统
·
电疗
·
抗菌研究
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DOI
高影响因子
危险品化学品经营许可证（带存储）