INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Bing Liu,Zhen-Zi Shang,Qiang-Ming Li,Xue-Qiang Zha,De-Ling Wu,Nian-Jun Yu,Lan Han,Dai-Yin Peng,Jian-Ping Luo
The structure features and anti-gastric cancer activities in vitro of stem, root, leaf and flower polysaccharides from cultivated Dendrobium huoshanense were investigated systematically. Stem polysaccharide (cDHPS) was composed of →4)-β-D-Glc p -(1→, →4)-β-D-Man p -(1→, →4)-3- O -acetyl-β-D-Man p -(1→ with the molecular weight of 2.59 × 10 5 Da; root polysaccharide (cDHPR) was composed of →3,5)-α-L-Ara f -(1→, →4)-β-D-Glc p -(1→, →4)-β-D-Man p -(1→, →4,6)-β-D-Man p -(1→, →6)-α-D-Gal p -(1→ and terminal β-L-Ara f with the molecular weight of 1.41 × 10 4 Da; leaf polysaccharide (cDHPL) was composed of →4)-β-D-Glc p -(1→, →4)-β-D-Man p -(1→, →4)-3- O -acetyl-β-D-Man p -(1→, →3,6)-β-D-Man p -(1→ and terminal α-D-Gal p with the molecular weight of 2.09 × 10 5 Da; and flower polysaccharide (cDHPF) was composed of →4)-β-D-Glc p -(1→, →4)-β-D-Man p -(1→, →3,6)-β-D-Man p -(1→ and terminal α-D-Gal p with the molecular weight of 4.78 × 10 5 Da. Among these four polysaccharides, cDHPS showed the best anti-gastric cancer activity evidenced by the inhibited growth and c-myc expression as well as the enhanced apoptosis and p 53 expression of murine forestomach carcinoma (MFC) cells, suggesting their difference in anti-gastric cancer activity should be contributed to their difference in structure features.
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高影响因子
JOURNAL OF FOOD SCIENCE
·
2021
·Vol. 86
·期 11
·4828-4839
·
IF 3.167
Jing-wen Hao,An-ling Zhu,Nai-Dong Chen,Xiao-quan Liu,Qiang Li,Hui-min Xu,Wei-Han Yang,Chao-feng Qin,Jin-Dong Wu
A phenylhexyl isothiocyanate (PITC) precolumn derivatization quantitative analysis of multicomponents by a single marker (QAMS) strategy for the simultaneous analysis of 20 free amino acids (FAA) in Dendrobium huoshanense is proposed. The method was validated by the linearity, limit of detection (LDO), and limit of quantitation (LOQ), recovery, precision, and stability. The results showed that when applying the established method, the LOQ of the FFAs was lower than 1 ng/ml except threonine (1.32 ng) and cysteine (1.16 ng). The QAMS investigation revealed that, using any one of the 20 FAAs as the reference internal standard, no significant differences were observed between the external standard method and the QAMS method for the quantification of FAAs in D. huoshanense by PITC precolumn derivatization [The relative standard deviation (RSD, %) by QAMS and ESM were all below 5%]. HPLC fingerprint investigation combined with similar analysis (the similarity values for S1–S25 were >0.875) and quality fluctuation analysis showed that the cultivation environment might have a great effect on the accumulation of FAAs in D. huoshanense . Overall, our study showed that we might increase the accuracy and scope of the simultaneous quantification of multicomponents using the QAMS technique by being derivatized with a strong UV absorbing group, and QAMS combined with chromatographic fingerprinting can be considered good quality criteria for the quality control of D. huoshanense and may provide analytical technical support for research on Maillard Reaction during the further processing of D. huoshanense .
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Ku-Ku Hao,Qi-Jin Wang,Sheng-Xiang Wei,Hua-Yang Si,Jing-Wen Hao,Nai-Fu Chen,Nai-Dong Chen,Xiao-Yan Gao,Si-Jie Liao,Shao-Jun Zheng,Man-Man Zhang
A neutral polysaccharide (NDHP-2-1) was isolated from Dendrobium huoshanense C. Z. Tang et S. J. Cheng ( D. huoshanense ). Structure characterization revealed that the weight-average molecular weight of NDHP-2-1 was 20.42 kDa, mainly composed of glucose (95.46 %) and mannose (4.54 %). The backbone of NDHP-2-1 consisted of →6)-α-D-Glc p -(1→, →4)-α-D-Glc p -(1→, and →3,4)-α-D-Glc p -(1→. The T-α-D-Glc p was regarded as the branches attached to the O-4 position of →3,4)-α-D-Glc p -(1 → in the backbone. In vitro experiments indicated that NDHP-2-1 exhibited excellent anti-inflammatory activity. The RAW264.7 cell assay results showed that NDHP-2-1 effectively inhibited the release of pro-inflammatory cytokines, including interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), interleukin-10 (IL-10) from LPS-stimulated macrophages. In general, the newly isolated polysaccharide of NDHP-2-1 exhibited significant anti-inflammatory and immunomodulatory properties, suggesting its potential as an effective natural therapeutic agent for managing inflammatory diseases.
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高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Chengying Hai,Wanjun Long,Yixin Suo,Huanhuan Lu,Hengye Chen,Xiaolong Yang,Jian Yang,Haiyan Fu
Dendrobium huoshanense (DHS) is an expensive Chinese herb with medicinal and edible functions. The quality of DHS is closely related to cultivation methods and years. In this work, nano-effect near and mid infrared spectra of 320 DHS samples with four cultivation methods and four years were obtained, and the feature vectors were obtained using a mid-level data fusion strategy with significant variable extraction and integration using variable importance for the projection (VIP). The feature vectors then combined with partial least squares discriminant analysis (PLS-DA) and orthogonal partial least squares discriminant analysis (OPLS-DA) to identify the cultivation years and methods. The result showed that it is difficult to discriminate effectively by a single spectrum, and the accuracy of PLS-DA discrimination based on raw or simple fusion of raw spectra is only 95.5% and 84.4% or 99.6% and 88.5%, while the accuracy of discrimination based on nano-effect feature fusion spectra can be improved to 100%, and PLSR was used to quantify the cultivation year of the DHS with good linearity and accuracy. The nano-effect spectrum is based on the reaction of tetraphenyl zinc porphyrin (ZnTPP) with polysaccharides , flavonoids and alkaloids in DHS, which amplifies the spectral difference. This study demonstrates that multi-spectral feature vectors fusion method could potentially be a reliable analytical method to distinguish the cultivation methods and years in DHS herbs.
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高影响因子
Plants-Basel
·
2025
·Vol. 14
·期 24
·3718
·
IF 4.1
Xinxin Li,Ziting Zhu,Jing Wu,Nianjun Yu,Huiqun Xie,Lan Han,Daiyin Peng
Dendrobium huoshanense , a rare and endangered medicinal orchid species endemic to Huoshan County, Anhui Province, faces a severe limit of wild resources. Its medicinal efficacy derives from unique chemical constituents, which vary significantly across cultivation modes. We systematically compared sensory properties and nutritional constituents (polysaccharides, flavonoids, alkaloids, bibenzyls, minerals, and free amino acids) between D. huoshanense in three cultivation modes—greenhouse cultivation, understory gravel cultivation, and wild-simulated cultivation. Additionally, the gastroprotective effects of wild-simulated cultivated D. huoshanense on gastric mucosal epithelial cells were evaluated. Results demonstrated that wild-simulated cultivation yielded significantly higher levels of nutritional constituents compared to gravel and greenhouse cultivation. Furthermore, wild-simulated cultivated D. huoshanense exhibited notable protective effects against gastric mucosal epithelial cell damage. This study not only elucidates the influence of cultivation modes on the chemical profile of D. huoshanense but also provides scientific evidence supporting its gastric protective properties, offering a foundation for its sustainable utilization and quality-oriented cultivation.
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高影响因子
CHEMISTRY & BIODIVERSITY
·
2022
·Vol. 19
·期 8
·e202200471
·
IF 2.745
Qiang-Ming Li,Xiu-Rong Yang,Xue-Qiang Zha,Li-Hua Pan,Dan-Dan Zang,Feng-Yun Zhang,Jian-Ping Luo
Dendrobium huoshanense flowers have been widely used for liver protection in China. This work was aimed to discover the natural products with activity of mitigating alcoholic hepatocyte injury from Dendrobium huoshanense flowers via bioactivity-guided isolation, and to clarify the underlying mechanisms of these natural products. As a result, three flavonoids, 3′- O- methylquercetin-3- O-β -D-galactopyranoside ( 1 ), 3′- O- methylquercetin-3- O-β -D-glucopyranoside ( 2 ) and quercetin-3- O-β -D-glucopyranoside ( 3 ), were firstly isolated from D. huoshanense flowers. Results exhibited that flavonoids 1 – 3 could enhance the cell viability, decrease the expression of ALT and AST, inhibit the cell apoptosis, alleviate the oxidative stress, and mitigate the inflammatory response of alcohol-induced L02 cells. Mechanism study exhibited that flavonoids 1 – 3 could increase the expression of Nrf2 as well as its downstream antioxidation genes of alcohol-induced L02 cells, while ML-385 (Nrf2 inhibitor) could abolish the inhibitory effects of 1 – 3 on alcohol-induced hepatocyte injury. Flavonoids 1 – 3 could also reduce the phosphorylation levels of I κ B α and NF- κ B p65 of alcohol-induced L02 cells, while SC75741 (NF- κ B inhibitor) could not enhance the inhibitory effects of 1 – 3 on alcohol-induced L02 cells injury. The data above indicated that flavonoids 1 – 3 could inhibit alcohol-induced hepatocyte injury, which might be attributed to alleviating oxidative stress and mitigating inflammatory response by activating Nrf2 and inhibiting NF- κ B pathways.
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高影响因子
PHYTOCHEMICAL ANALYSIS
·
2020
·Vol. 31
·期 3
·366-374
·
IF 2.772
Jing-Wen Hao,Yue Chen,Nai-Dong Chen
In this study, the potentiality of applying attenuated total reflectance near-infrared (ATR-NIR) and attenuated total reflectance mid-infrared (ATR-MIR) techniques combined with a partial least squares (PLS) regression technology to quantify the total polyphenols (TPs) in Dendrobium huoshanense (DHS) was investigated and compared. The real TP contents in the DHS samples were analysed using methods of reference. The capability of the two IR spectroscopic techniques to quantify the TPs in DHS was assessed by the root-mean-square error of calibration (RMSEC) and determination coefficients ( R 2 ). The results showed that both NIR and MIR might be used as a fast and simple tool to replace traditional chemical assays for the determination of the TP contents in DHS, and the best NIR model showed slightly better prediction performance [root-mean-square error of prediction (RMSEP): 0.307, R 2 : 0.9122, ratio performance deviation (RPD): 4.43] than the best MIR model (RMSEP: 0.440, R 2 : 0.9069, RPD: 3.09). Results from this study indicated that both the NIR and MIR models could be used to quantify the TP in DHS, and ATR-NIR appeared to be the more predominant and more robust technique for the quantification of the TP in DHS.
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CHEMISTRY & BIODIVERSITY
·
2025
·
IF 2.5
Sheng-Xiang Wei,Jie Wang,Qi-Jin Wang,Xiao-Yan Gao,Jun Chen,Ku-Ku Hao,Nai-Dong Chen,Xuan-Xuan Fan,Ya-Feng Dai
One previously undescribed C 22 -diterpenoid alkaloid (DHS-DA-1) was isolated from Dendrobium huoshanense C. Z. Tang et S. J. Cheng. The structure of the new alkaloid was elucidated using extensive spectroscopic analyses and NMR calculations. Griess reaction results showed that the new compound could reduce the creation of nitric oxide (NO) in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages, and the ELISA experiments suggested that DHS-DA-1 could efficaciously decrease LPS-induced inflammation by inhibiting tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) expressions. In addition, DHS-DA-1 could effectively inhibit the survival and proliferation of AGS cells and promote their apoptosis in a concentration-dependent manner. The stability and feasibility of binding the DHS-DA-1 to the target protein are further verified by molecular docking and molecular dynamics simulation validation. In addition, DHS-DA-1 exhibits favorable ADMET properties. Graphical Due to the limited space in the graphical abstract, only a portion of the article's content is presented. A diterpenoid alkaloid with 22 carbon atoms was isolated from the stems of Dendrobium huoshanense , exhibiting certain inhibitory effects on the inflammatory factor IL-6 and human gastric adenocarcinoma cells (AGS). The stability and feasibility of the binding between DHS-DA-1 and the target protein (5FUC) were further validated through molecular docking and molecular dynamics simulations.
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JOURNAL OF ETHNOPHARMACOLOGY
·
2026
·
IF 6.8
Xin Gao,Zhen-Zi Shang,Qiang-Ming Li,Feng-Yun Zhang,Xue-Qiang Zha,Jian-Ping Luo
Ethnopharmacological relevance Dendrobium huoshanense has been long used to treat gastrointestinal disorders including ulcerative colitis (UC) in traditional Chinese medicine. Polysaccharides as its main active components can alleviate UC by reducing inflammation, and promote the secretion of glucagon-like peptide-1 (GLP-1), which possesses anti-inflammatory effects via GLP-1 receptor (GLP-1R). However, it remains unclear whether polysaccharides from different parts of this herb are equally effective in alleviating UC and whether polysaccharides function via GLP-1/GLP-1R axis. Aim of the study To identify the part in which the polysaccharide is most effective in alleviating UC and to explore its mechanism of action through GLP-1/GLP-1R axis. Materials and methods The physicochemical properties of polysaccharides were analyzed. A dextran sulfate sodium (DSS)-induced UC mouse model was used to assess colitis-associated damage via morphological scoring, histopathological evaluation and inflammatory examination with multi-cytokine array and western blotting. Gut microbiota compositions, short-chain fatty acid contents and GLP-1 levels were measured, and their roles were checked using antibiotic mixture and GLP-1R antagonist Exendin (9-39). Results Stem, leaf and flower polysaccharides, with different molecular weight, monosaccharide composition and spatial conformation, differentially alleviated UC symptoms, and stem polysaccharide (DHPS) showed the strongest efficacy, as evidenced by improved epithelial integrity, mucus barrier and inflammatory disorder. DHPS-alleviated UC was independent of DHPS-altered gut microbiota and positively correlated with DHPS-elevated GLP-1 levels, which could be abolished by Exendin (9-39), suggesting that GLP-1-activated GLP-1R signaling mediated the effects of DHPS against UC. Conclusions DHPS is the most effective polysaccharide for alleviating DSS-induced UC through GLP-1/GLP-1R axis, proposing DHPS is a valid candidate for UC treatment using D. huoshanense .
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高影响因子
SPECTROSCOPY LETTERS
·
2019
·
IF 1.081
Jing-Wen Hao,Nai-Dong Chen,Xu-Cheng Fu,Jian Zhang
An attenuated total reflectance Fourier transform infrared spectroscopy method combined with partial least square regression was established to rapidly quantify the total polysaccharide and its major monosugars in Dendrobium huoshanense. The results showed that the optimal predictive methods were the models established by the infrared spectroscopy files pretreated by standard normal transformation combined with the second derivative; the accuracy of the models selecting wavelength regions based on the assignments of Fourier transform infrared spectroscopy signals of polysaccharide was obviously higher than the models selecting wavelength regions suggested by Thermofisher Quantity Analyst software and other possible wavelength region selecting modes. The external validation and the complete external validation confirmed the robustness and reliability of the developed attenuated total reflectance Fourier transform infrared model. Our study might provide an efficient technique tool for the rapid, green, low-cost, and nondestructive quantification of the total polysaccharide and the main monosaccharides in Dendrobium huoshanense and other rich-in-polysaccharide plant food or medicines.
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高影响因子
MOLECULES
·
2024
·Vol. 29
·期 12
·2791
·
IF 4.2
Haijun Xu,Zhu Liu,Wen Xu,Yafei Zhang
The extract ofDendrobium huoshanense, a traditional Chinese medicinal and food homologous plant belonging to the family Orchidaceae, was previously reported to have hypoglycemic and antioxidant effects. In this study, the direct effects of polysaccharide (DHP) and non-polysaccharide (NDHP) components ofD. huoshanense, as well as its water extract (DHWE) were compared with that of metformin (an antidiabetic drug) on the gut microbiota (collected from fecal flora) of rats with streptozotocin-induced type 1 diabetes (T1D) using an in vitro fermentation method. The results showed that DHWE, DHP, and NDHP reduced pH and increased bacterial proliferation and short-chain fatty acid (SCFA) content in fermentation broth. DHWE, DHP, NDHP and metformin promoted the production of acetic and propionic acid, acetic acid, propionic acid and butyric acid, and propionic acid, respectively. DHWE, DHP, and NDHP reduced the abundance ofProteobacteria(subdominant pathogenic bacteria) and increased the abundance ofFirmicutes(dominant beneficial gut bacteria). NDHP also reduced the abundance ofBacteroidetes(beneficial and conditional pathogenic). Metformin increased the abundance ofProteobacteriaand reduced the abundance ofFirmicutesandBacteroidetes. At the genus level, NDHP promoted the proliferation ofMegamonasandMegasphaeraand decreased harmful bacteria (e.g.,Klebsiella), and DHP increased the abundance ofPrevotellaceae(opportunistic and usually harmless). By contrast, metformin increased the abundance of harmful bacteria (e.g.,Citrobacter) and reduced the abundance of beneficial bacteria (e.g.,Oscillospira). Our study indicates that DHWE, DHP, and NDHP are potentially more beneficial than metformin on the gut microbiota of T1D rats in vitro.
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