JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2024
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IF 2.8
Yiwen Zhang,Yang Guo,Liang Song,Wenshuai Liu,Rui Nian,Xiying Fan
This study introduces an efficient on-column refolding and purification method for preparing nanobodies (Nbs) expressed as inclusion bodies and fusion proteins. The HisTrap TM FF system was successfully employed for the purification of the fusion protein FN1-ΔI-CM-2D5. The intein ΔI-CM cleavage activity was activated at 42 °C, followed by incubation for 4 h. Leveraging the remarkable thermal stability of Nbs, 2D5 was further purified through heat treatment at 80 °C for 1h. This method yielded up to 107.2 mg of pure 2D5 with a purity of 99.2 % from just 1L of bacterial culture grown in a shaker flask. Furthermore, this approach successfully restored native secondary structure and affinity of 2D5. Additionally, the platform was effectively applied to the refolding and purification of a polystyrene-binding nanobody (B2), which exhibited limited expression in the periplasmic and cytoplasmic spaces of E. coli . This endeavor resulted in the isolation of 53.2 mg of pure B2 Nb with a purity exceeding 99.5 % from the same volume of bacterial culture. Significantly, this approach restored the native secondary structure of the Nbs, highlighting its potential for addressing challenges associated with expressing complex Nbs in E. coli . Overall, this innovative platform provides a scientifically rigorous and reproducible method for the efficient preparation of Nbs, offering a valuable tool for antibody research and development.
研究领域:
生物技术
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蛋白质工程
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分子生物学
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抗体研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
DALTON TRANSACTIONS
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2023
·Vol. 52
·期 40
·14435-14442
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IF 4
Qihao Wu,Tao Xie,Li Zhang,Hui Ding,Heju Gao,Jiahui Jiang,Guancheng Xu
To achieve broad commercialization of rechargeable metal–air batteries, the development of non-precious metal-based bi-functional oxygen electrocatalysts is critical. In this study, we prepared N,S co-doped porous carbon materials containing Co9S8 nanoparticles (Co9S8/NSC) through a one-step pyrolysis process. The process involved the pyrolysis of a polydopamine (PDA) coated Co-formic acid framework (Co-FF) derived Co3O4 and thiourea. The improved catalyst Co9S8/NSC-1 exhibited satisfactory long-term durability and superior oxygen reduction reaction (ORR) and oxygen evolution reaction (OER) activity, the half-wave potential (E1/2) of the ORR reached 0.83 V, and the OER overpotential at 10 mA cm−2 (η10) was 300 mV. The zinc–air battery (ZAB) based on Co9S8/NSC-1 assembly had a maximum power density of 102.0 mW cm−2 and the cycle life reached 500 cycles. The material preparation method was simple, environmentally friendly and inexpensive, providing a feasible strategy for the development of non-precious metal-based electrocatalysts.
研究领域:
材料科学
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电化学
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能源存储与转换
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry A
·
2020
·Vol. 9
·期 1
·413-422
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IF 11.301
Xiaohuan Chang,Junjie Fu,Dongxing Kou,Wenhui Zhou,Zhengji Zhou,Shengjie Yuan,Yafang Qi,Zhi Zheng,Sixin Wu
The introduction of alkali metal (AM) post-deposition treatment (PDT) has led to the consecutive breakthrough of world record efficiencies for Cu(In,Ga)Se2 photovoltaics. However, for the derivative Cu2SnZn(S,Se)4 (CZTSSe) solar cells, the corresponding verdict seems partially unjustified due to the difference in defect chemical environment and phase structure, and the most effective incorporation means of AMs is still a mystery. In this work, for the first time, we developed a dual AM synergistic incorporation strategy combining light and heavy AMs by post-depositing CsF after NaF-PDT to improve the efficiency of CZTSSe solar cells. The relevant microscopic-electrical advantages with regard to the different roles of NaF and CsF PDT are discussed in more detail. It was found that the Na atoms are randomly dispersed both in the grain interior (GI) and grain boundaries (GBs), significantly increasing the acceptor concentration and passivating the deep level defects to enhance the Voc. In contrast, the Cs atoms are mainly segregated at the GBs, primarily increasing the potential of the GBs and improving the FF. The prominent electric benefits of Cs at the GBs and Na in the GI finally increase the device efficiency from 10.12% to 12.16%. Our findings solve the difficulty in obtaining the available Voc and FF for future high efficiency research and should serve as a basis for the in-depth understanding of AM-PDT in kesterite photovoltaics.
研究领域:
材料科学
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光伏技术
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半导体物理
✓ 已验证
产品关联
DOI
高影响因子
MATERIALS SCIENCE IN SEMICONDUCTOR PROCESSING
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2020
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IF 3.085
Zhou Liu,Shuzhen Wu,Xiaojie Yang,Yijun Zhou,Jiaren Jin,Junmei Sun,Li Zhao,Shimin Wang
SnO 2 has attracted extensive attention in planar perovskite solar cells (PSCs) owing to its outstanding photoelectric properties and low temperature preparation. However, pinholes are easy to appear in the annealing process of SnO 2 films, which limits its application in planar PSCs. Here lists a simple and low-cost method: Urea-complexed tin oxide in deionized water can effectively control the concentration of SnO 2 . This process is conducive to forming a compact surface morphology . Meanwhile, the work explored the effect of the properties of PSCs with different thicknesses of SnO 2 layers. The system analysis showed that PSCs based on 60 nm SnO 2 layer and the methamphetamine lead iodide (MAPbI 3 ) light absorber yielded the highest power conversion efficiency (PCE) over 18%, a fill factor ( FF ) of 0.793, and good stability. Therefore, we believe our study provides an effective way for preparing electron transport layers.
研究领域:
材料科学
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可再生能源
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太阳能电池
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光伏技术
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）