TALANTA
·
2023
·
IF 6.1
Feifei Ma,Zhe Zhao,Jiahui Huang,Qing Xiong,Shaohua Xu,Zhenyu Lin
The level of human chorionic gonadotropin (HCG) is an important indicator for early pregnancy, pregnancy-related diseases trophoblastic diseases and even cancer diagnosis. Therefore, sensitive detection of HCG has crucial significance in clinical, especially in gynaecology and obstetrics. Herein, a hybridization chain reaction (HCR) assisted multicolor immunosensor have been developed for HCG analysis. The proposed method introduced HCR after the immunoreaction between antibody and HCG protein, and produced long double strand DNA (dsDNA) that contain biotin sites. The streptavidin-horseradish peroxidase was linked on the dsDNA by the interaction between biotin and streptavidin, and can further mediated gold nanobipyramids (Au NBPs) etching. The localized surface plasmon resonance absorption peaks of Au NBPs blue shift and accompanied a vivid color change after etching effect. Based on this color change, HCG could be qualitative and semi-quantitative detected. Because of the introduction of HCR and enzyme amplification technique, the proposed method exhibited high sensitivity with a linear range of 0.1–2000 pg/mL and limit of detection (LOD) of 0.1 pg/mL. Finally, the proposed immunosensor was used to detect clinical serum samples. The results show there are no significant differences between clinical results and the test results by this method, indicating the practicability of the proposed method.
研究领域:
临床化学
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生物传感器
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分析化学
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生物医学工程
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DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2024
·
IF 10.7
Junpeng Lv,Jianwen Wan,Di Wu,Xiyao Zhang,Wenhui Xu,Mengge Wang,Songmin Chen,Zhuojun Ye,Yiyan Tian,Qiong Hu,Dongxue Han,Li Niu
As a glycoprotein hormone, human chorionic gonadotropin (hCG) is an established marker for pregnancy test. On the basis of the target-mediated silver deposition (TSD), in this work, we report the development of an amplification-free electrochemical biosensor for the highly sensitive detection of hCG. The detection of hCG involves the use of the affinity peptide-modified electrode for hCG capture (the CGGSSPPLRINRHILTR peptide containing the hCG-binding domain of the PPLRINRHILTR sequence is used as the affinity peptide), the oxidation of the diol sites of the glycan chains on hCG hormones into aldehyde groups by NaIO 4 , and the deposition of silver nanoparticles (AgNPs) for the solid-state voltammetric stripping analysis. Due to the deposition of multiple AgNPs while the solid-state Ag/AgCl voltammetric process has a high signal-to-noise ratio, the TSD-based electrochemical biosensor can be applied to the highly sensitive detection of hCG without the need for signal amplification. Under optimal conditions, the stripping current increased linearly with an increasing hCG concentration over the range from 1.0 to 25 mIU/mL, with a detection limit of 0.45 mIU/mL. Owing to the high specificity of the hCG-binding peptide PPLRINRHILTR, this electrochemical hCG biosensor exhibits high selectivity. The results of the quantitative assay of hCG in urine samples at the concentrations of 25, 10, and 1.0 mIU/mL are desirable, indicating the good anti-interference capability. As the TSD-based electrochemical biosensor allows the amplification-free detection of low-abundance hCG, it is easy to use and cost-effective, showing great promise in point-of-care assay of hCG for pregnancy test.
研究领域:
电化学
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生物传感器
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分析化学
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医学诊断
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Jian Yang,Xinli Liu,Yongchun Pan,Jingjing Yang,Bangshun He,Yunzhi Fu,Yujun Song
Sandwich immunoassay plays a significant role in disease diagnostics and prognosis. However, this technology always requires cumbersome manual washing procedures and well trained operators. To address the issues, we made an effort to develop a self-powered microfluidic chip (SP-Chip) to accomplish multiple washing processes and antigen-antibody binding reaction automatically in one-step sliding manner. This smart microfluidic platform integrated with fluorescence microscopic counting technique for rapid and quantitative detection of human chorionic gonadotropin (hCG) and carcinoembryonic antigen (CEA) in 10 min. Moreover, the versatile strategy is not only applied to various biomarkeres detection, but also suitable for designing other sample-in-answer-out diagnostic system. Taken together, the autonomous, accurate, inexpensive, self-contained, and easy to use chip lays a solid foundation for quantitative detection of biomarkers in the fields of disease treatment and personalized diagnostics.
研究领域:
生物医学工程
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分析化学
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诊断学
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产品关联
DOI
高影响因子
Analytical Methods
·
2021
·Vol. 13
·期 38
·4442-4451
·
IF 2.896
Huanhuan Li,Tongji Cai,Yi Ren,Jing Huang,Hanbing Jiang,Yucui Hou,Chunhua Tang,Jie Yang,Jia Zhao,Peng Yu
As an essential biochemical indicator in the fields of pregnancy and oncology, human chorionic gonadotropin (HCG) can be evaluated using colloidal gold immunochromatographic paper and quantified using a biochemical analyzer based on the principle of the antibody sandwich method. In view of the inaccuracy of the former and the complication of the latter, this study constructed an accurate, sensitive and simple unlabeled biosensor based on peptide aptamer CGGGPPLRINRHILTR for HCG detection. Molecular Operating Environment (MOE) was used to simulate the aptamer and protein docking, and western blot (WB) was used to verify the binding effect and ratio. The peptide aptamer was characterized and was then reduced with tris-(2-carboxyethyl)-phosphine hydrochloride (TCEP). After electrochemical deposition of chloroauric acid on the screen-printed electrode (SPE), the aptamer was self-assembled on the electrode surface under optimal conditions. The active site of the electrode surface was blocked with 6-mercapto-1-hexanol (MCH) and BSA. The electrochemical impedance spectrum (EIS) was used to quantify HCG in the matrix. Showing a good linear relationship in the range of 5–1500 mIU mL−1, with a detection limit of 1 mIU mL−1, the biosensor remained stable at room temperature for 14 days. Because of its small size, stability, sensitivity and accuracy, this biosensor has great potential to become a portable diagnostic device for HCG.
研究领域:
生物化学
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生物传感器
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分析化学
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妊娠诊断
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肿瘤学
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产品关联
DOI
PubMed
高影响因子
LANGMUIR
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2011
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IF 4.269
Xian’an Zhang,Weifeng Lin,Shengfu Chen,Hong Xu,Hongchen Gu
To overcome major challenges of non-specific protein adsorption on nanoparticles for nanosensing and nanodiagnosis, an efficient method for robust chemical modification was developed to achieve excellent specific biorecognition and long-term stability in complex biomedia. This method is demonstrated by a highly specific and sensitive immunoassay (IA), using superparamagnetic nanospheres (NSs) with high magnetite content. The non-specific protein adsorption on the NSs was suppressed dramatically when modified with dual functional poly(carboxybetaine methacrylate) (polyCBMA) via surface-initiated atom transfer radical polymerization (SI-ATRP) and chemically grafted with antibodies of the β subunit of human chorionic gonadotrop (anti-β-hCG). The response to hCG of IA NSs with polyCBMA coatings was highly consistent in either phosphate-buffered saline (PBS) or 50% fetal bovine serum (FBS), which is far less variable than the response of the IA NSs without polyCBMA coatings. After all, a very robust platform for IA NSs with excellent specific biorecognition was obtained. It is expected that this method for nanoparticle modification could be widely used in ultrasensitive nanosensing and nanodiagnosis in the future.
研究领域:
纳米技术
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生物材料
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生物医学工程
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分析化学
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产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2021
·Vol. 45
·期 24
·10618-10625
·
IF 3.591
Qianrui Xu,Yang Wang,Pengcheng Gao,Yong Jiang
Fluorescent nanobeads are widely used due to their advantages of visualization, sensitivity and the quantitative measurement of target analytes. In terms of their applications, the stability and biocompatibility of fluorescent nanobeads are very important. In this work, “pomegranate”-like QD/SiO2/poly(St-co-MAA) (QSIP-COOH) nanobeads were synthesized by two-step strategies. First, quantum dots (QDs) were covered by a silica layer using the reverse hydrolysis method to form a core–shell structure like the seed and flesh of a pomegranate, and then a poly(St-co-MAA) layer was formed as the skin of the pomegranate. The structure and morphology of nanobeads were investigated by FTIR, TG, TEM and HRTEM. The results confirmed that QSIP-COOH nanobeads had a pomegranate structure with a QD core, an intermediate silica shell, and a carboxylated polystyrene outermost shell. TEM and HRTEM results showed that the size of the typical product was about 170 nm with the QDs of about 8 nm and the MPS-modified QD/SiO2 (QSI-CC) of about 27 nm, respectively. The in vitro cytotoxicity test showed that nanobeads had desirable stability and good biocompatibility, which made them applicable for various bioassays and diagnostics. Here, QSIP-COOH fluorescent nanobeads were applied to immunochromatography test strip (ICTS) and the result proved that QSIP-COOH fluorescent nanobeads could be used for the sensitive detection of immune human chorionic gonadotropin (HCG).
研究领域:
材料科学
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纳米技术
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生物医学工程
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分析化学
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Yuqian Guo,Yaofeng Zhou,Sicheng Xiong,Lifeng Zeng,Xiaolin Huang,Yuankui Leng,Yonghua Xiong
The usage of nanozyme as label to establish immunoassays offers merits of low cost and high robustness. However, the catalytic activity of nanozyme is usually passivized by the modified layer of bio-recognition molecules. The peroxidase-mimicking activity of cetyltrimethylammonium bromide (CTAB)-coated gold nanoparticles (AuNPs@CTAB) is recovered by Ag + ions. Herein, AuNPs@CTAB with Ag + -triggered catalytic activity was first designed as signal transducer to construct colorimetric immunoassay for human chorionic gonadotropin (HCG) detection. Silver nanoparticles were used as labels which can release numerous Ag + ions by hydrogen peroxide corrosion and thus enhance the catalytic activity of AuNPs@CTAB. To achieve the highest detection sensitivity, we synthesized three kinds of AuNPs@CTAB with similar size but different shapes including nanospheres (AuNSs@CTAB), nanorods (AuNRs@CTAB), and nanocubes (AuNCs@CTAB). The morphology-dependent catalytic properties of AuNPs@CTAB were evaluated. Under optimum conditions, the proposed method using AuNSs@CTAB, AuNRs@CTAB and AuNCs@CTAB using AuNSs@CTAB, AuNRs@CTAB and AuNCs@CTAB gave LOD of 7.8, 15.6 and 31.2 mIU/mL, respectively. The developed immunoassays also showed good accuracy, high reproducibility and high robustness for detecting HCG in serum samples. In summary, the developed immunoassay is a promising bioanalysis platform which can provide similar sensitivity and enhanced robustness compared with traditional horse radish peroxidase-based immunoassays.
研究领域:
生物化学
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分析化学
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纳米技术
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免疫分析技术
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DOI
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 8
·1-9
·
IF 5.479
Zhang Zhi,Xu Guoli,Xie Li,Guan Yueping
A colorimetric immunoassay is described for the pregnancy marker human chorionic gonadotropin (HCG). The assay is based on the use of MnO 2 nanorods acting as a peroxidase mimic. The nanorods were prepared by a hydrothermal method using EDTA as a template. They exhibit excellent peroxidase-like activity and stability. The nanorods were immobilized in a chitosan matrix in the wells of a BSA-modified microplate which then were further modified with streptavidin and biotinylated capture antibodies. The specific recognition between HCG and the antibodies in wells inhibit the peroxidase-like activity of the nanorods. Hence, the substrate 3,3′,5,5′-tetramethylbenzidine is less efficiently catalytically oxidized by H 2 O 2 to form a blue colored product. This results in a decrease in the absorbance at 652 nm. Response is linear in the 0.5 to 400 mIU·mL −1 HCG concentration range, and the detection limit is 0.36 mIU·mL −1 under optimized conditions. The method is highly specific, acceptably reproducible and stable. It was applied to the determination of HCG concentration in serum samples, and results were in good agreement with data obtained by the reference method.”
Schematic presentation of colorimetric immunoassay for human chorionic gonadotropin (HCG) by using MnO 2 nanorods with peroxidase-like activity immobilized in microplate wells. The specific recognition between HCG and the antibodies in wells inhibits the peroxidase-like activity of the nanorods. TMB: 3,3′,5,5′-tetramethylbenzidine.
研究领域:
分析化学
·
生物化学
·
纳米技术
·
医学诊断
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产品关联
DOI
PubMed
高影响因子
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