PLoS One
·
2016
·Vol. 11
·期 9
·e0161566
·
IF 3.057
Ming Guo,Xiaomeng Wang,Xiaowang Lu,Hongzheng Wang,Peter E. Brodelius
In order to obtain the biological active compound, α-mangostin, from the traditional native mangosteen ( Garcinia mangostana L.), an extraction method for industrial application was explored. A high yield of α-mangostin (5.2%) was obtained by extraction from dried mangosteen pericarps with subsequent purification on macroporous resin HPD-400. The chemical structure of α-mangostin was verified mass spectrometry (MS), nuclear magnetic resonance ( 1 H NMR and 13 C NMR), infrared spectroscopy (IR) and UV-Vis spectroscopy. The purity of the obtained α-mangostin was 95.6% as determined by HPLC analysis. The binding of native α-mangostin to human serum albumin (HSA) or transferrin (TRF) was explored by combining spectral experiments with molecular modeling. The results showed that α-mangostin binds to HSA or TRF as static complexes but the binding affinities were different in different systems. The binding constants and thermodynamic parameters were measured by fluorescence spectroscopy and absorbance spectra. The association constant of HSA or TRF binding to α-mangostin is 6.4832×10 5 L/mol and 1.4652×10 5 L/mol at 298 K and 7.8619×10 5 L/mol and 1.1582×10 5 L/mol at 310 K, respectively. The binding distance, the energy transfer efficiency between α-mangostin and HSA or TRF were also obtained by virtue of the Förster theory of non-radiation energy transfer. The effect of α-mangostin on the HSA or TRF conformation was analyzed by synchronous spectrometry and fluorescence polarization studies. Molecular docking results reveal that the main interaction between α-mangostin and HSA is hydrophobic interactions, while the main interaction between α-mangostin and TRF is hydrogen bonding and Van der Waals forces. These results are consistent with spectral results.
研究领域:
药用植物学
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药物化学
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生物化学
·
分子生物学
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DOI
PubMed
高影响因子
Metallomics
·
2024
·
IF 2.9
Shi Jia,Xie Leilei,Gong Wenjun,Bai Hehe,Wang Wenming,Wang Ai,Cao Wei,Tong Hongbo,Wang Hongfei
Four Ru(II)-centered isomeric complexes [RuCl(5cqn)(Val)(NO)] (1-4) were synthesized with 5cqn (5-chloro-8-hydroxyquinoline) and chiral Val (Val = L- or D-valine) as co-ligand, and their structures were confirmed using the X-ray diffraction method. The cytotoxicity and photodynamic activity of the isomeric complexes and their human serum albumin (HSA) complex adducts were evaluated. Both the isomeric complexes and their HSA complex adducts significantly affected HeLa cell proliferation, with an IC50 value in the range of 0.3–0.5 μM. The photo-controlled release of NO in solution was confirmed using time-resolved FT-IR and EPR spectroscopy techniques. Furthermore, photoinduced NO release in living cells was observed using a selective fluorescent probe for NO. Moreover, the binding constants (Kb) of the complexes with HSA were calculated to be 0.17–1.98 × 104 M−1 and the average number of binding sites (n) was found to be close to 1, it can serve as a crucial carrier for delivering metal complexes. The crystal structure of the HSA complex adduct revealed that one [RuCl(H2O)(NO)(Val)]+ molecule binds to a pocket in domain I. This study provides insight into possible mechanism of metabolism and potential applications for nitrosylruthenium complexes.
研究领域:
无机化学
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药物化学
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癌症研究
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生物物理化学
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DOI
PubMed
高影响因子
Epigenomics
·
2022
·
IF 4.357
Hanlin Li,Yanjie Yang,Jianpeng Yu,Boya Zhang,Xuanrong Chen,Shimiao Zhu,Yuanjie Niu,Zhiqun Shang
Aims: We aimed to determine whether intronic circRNA acts as a molecular sponge in castration-resistant prostate cancer (CRPC). Materials & methods: A gene chip technique was used to conduct sequencing. A qPCR experiment was performed to verify the result. Radioimmunoprecipitation, RNA pull-down and dual-luciferase reporter assays were performed to particularly expound its function. Verification of downstream effects was carried out through qPCR and western blot, and a xenograft assay was performed in vivo for verification. Results: Intronic circRNA hsa_circ_0092339 in the nucleus was highly expressed in CRPC cell lines. hsa_circ_0092339 did not regulate the expression of its parental gene. hsa_circ_0092339 functions like a molecular sponge, preventing degradation of C-MYC mRNA by absorbing hsa-mir-940. Conclusion: hsa_circ_0092339 plays a critical role in CRPC through targeting C-MYC indirectly by absorbing hsa-mir-940.Plain language summaryOur research breaks the mold by investigating a novel function of RNA and a novel regulatory mechanism. Our research provides a new therapeutic target for prostate cancer treatment and broadens the understanding of prostate cancer.
研究领域:
肿瘤学
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分子生物学
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癌症研究
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前列腺癌
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DOI
PubMed
高影响因子
MEDICINAL CHEMISTRY RESEARCH
·
2017
·Vol. 27
·期 3
·944-953
·
IF 1.277
Wang Zechun,Han Xinxin,Wang Ning,Wang Ruiyong,Chang Junbiao
Clenbuterol (CLB) is a notorious “lean meat essence”, which has harmful effects on human health. In this study, the binding of CLB to two proteins, human serum albumin (HSA) and the human fat mass and obesity associated protein (FTO), have been investigated by spectroscopic and molecular modeling methods. Fluorescence data revealed that CLB could bind to HSA (or FTO) to form a complex. Thermodynamic analysis and molecular modeling indicated that the binding process was a spontaneous molecular interaction procedure, in which hydrophobic interaction played a major role. Results indicated that CLB could lead to some microenvironmental changes of both proteins.
研究领域:
生物化学
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药物学
·
分子生物学
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DOI
高影响因子
JOURNAL OF CHEMICAL THERMODYNAMICS
·
2022
·
IF 3.269
Xiangyu Xu,Wenbo Li,Kai Chen,Jinlin Zhang,Linqing Yang,Zhongyu Du,Xuyan Mao,Min Liu,Yunfei Wang
Protein molecules tend to be adsorbed on the surfaces of nanoparticles, thereby easily forming nanoparticle protein corona (NPC). Herein, the foundational study of NPC formation of human serum albumin (HSA) absorbed on the surfaces of reduced graphene oxide sliver nanocomposites (rGO-AgNCs) was studied using UV–visible, fluorescence, and circular dichroism (CD) spectroscopy. When the rGO-AgNCs were added to the HSA solution, the adsorption spectra of HSA showed a slight blue shift, which indicated the formation of NPC. The fluorescence data revealed that the static quenching procedure was the main quenching mechanism. Also, Δ G θ < 0 indicated that the absorption process was spontaneous in thermodynamics, and HSA tends to bind to the surfaces of the rGO-AgNCs. Furthermore, Δ H θ < 0 and Δ S θ < 0 suggested that the binding was an enthalpy-driven process, and the main forces were the van der Waals forces and hydrogen bonds. The results of the synchronous fluorescence, three-dimensional emission experiments, and the α -helix content decrement indicated that the rGO-AgNCs slightly altered the HSA conformation. The morphological investigation and analysis using scanning probe microscopy (SPM) demonstrated that the NPC was formed on the surfaces of rGO-AgNCs.
研究领域:
生物化学
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纳米生物学
·
分子医学
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DOI
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2023
·
IF 2.9
Honglie Chen,Kun Yang,Shengbo Sang,Xing Guo,Yang Ge,Haoyu Wang,Pengli Xiao,Xiushan Dong,Dong Zhao
The conventional mechanical biosensor based on stress and electrical conversion can be an effective method to detect key human biomarkers for clinical diagnosis and early disease prevention. However, the applications of this type of biosensor are greatly limited due to their unsatisfactory sensitivity. In this work, a magnetic-sensitized (MS) mechanical biosensor based on multi-field coupling was developed for higher sensitivity, giving access to detect human serum albumin (HSA). Via introducing secondary magnetic antibodies labeled with magnetized Fe 2 O 3 nanoparticles to the stress and electrical conversion element of the MS-biosensor, the multi-field coupling was realized based on stress, electricity, and magnetism. Under the action of the magnetic field, the magnetic force of the secondary magnetic antibody and the stress of antigen-antibody binding jointly drove and enhanced the deformation of the MS-biosensor, amplifying the electrical signal, and realizing magnetic sensitization. The HSA was detected by the MS-biosensor at a range of 0–80 μg/mL with a limit of detection (LOD) of 0.14 μg/mL, demonstrating the high performance of the MS-biosensor. Moreover, the MS-biosensor showed high selectivity, specificity, and stability, indicating that the magnetic sensitization strategy of the MS-biosensor was significant for the clinical application of mechanical biosensors.
研究领域:
生物医学工程
·
临床诊断
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DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2015
·
IF 4.169
Qi-Ci Wu,Dong-Qiang Lin,Wei Shi,Qi-Lei Zhang,Shan-Jing Yao
Mixed-mode chromatography (MMC) is a new technology that uses specially-designed ligands to improve the adsorption selectivity with multimodal protein–ligand interactions for protein separation. A new MMC resin TA-B-6FF with tryptamine as the functional ligand was prepared and used for human serum albumin (HSA) separation. Adsorption equilibria of plasma-derived HSA (pHSA) were investigated and compared with a commercial tryptophan-based resin (MX-Trp-650m), and the influence of pH and salt addition was studied. The results showed that weak acidic conditions (pH 5.0–7.0) were favorable for HSA adsorption. The maximum adsorption capacity of TA-B-6FF was 141.33 mg/g at pH 5.0, which was two times higher than that of MX-Trp-650m. TA-B-6FF also showed better salt-tolerance than MX-Trp-650m. Moreover, TA-B-6FF was used to separate recombinant HSA (rHSA) from Pichia pastoris culture broth. The results indicated that rHSA could be directly captured by TA-B-6FF without dilution or pH adjustment. High purity (87.75%) of rHSA monomer could be obtained with a recovery of 98.53% through two-step elution process. Total content of rHSA monomer and degraded fragment was 99.75%, the removal of host cell proteins reached about 90%. The results demonstrate that new TA-B-6FF resin has a great potential for rHSA purification directly from the complex fermentation broth.
研究领域:
生物化学
·
蛋白质纯化
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生物技术工程
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DOI
PubMed
高影响因子
Water
·
2025
·Vol. 17
·期 5
·631
·
IF 3
Weiyuan Cao,Zixuan Yang,Ren Liu,Zilin Zhang,Guokuan Chen,Zilin Zhou,Liwei Xu
A novel composite biomass aerogel adsorbent (HSA) was prepared by dual physical and chemical cross-linking using sodium alginate (SA) as an organic biomass template and hydroxyapatite nanowires (HAPNWs) as an inorganic biomass skeleton. The structure of the HSA was characterized by scanning electron microscopy (SEM), X-ray powder diffractometry (XRD), Fourier transformed infrared spectroscopy (FTIR), and stress testing. One-factor experiments were conducted focusing on adsorption conditions at a Pb ion concentration of 300 mg/L, and the adsorption conditions were optimized using the response surface method. The optimal conditions obtained by numerical optimization using Design-Expert 13 were as follows: pH of 7.23, adsorption temperature of 35.42 °C, and adsorption time of 1050.73 min; the optimal adsorption capacity was 278.874 mg/g. To further reveal the adsorption mechanism of HSA, its adsorption model and kinetics were analyzed. Adsorption was most consistent with the Langmuir isothermal adsorption model, while the kinetics were most consistent with the pseudo-secondary kinetic model. R2reached 0.9986, indicating a mono-molecular layer of adsorption by heat, while the main adsorption mechanism was chemisorption.
研究领域:
环境科学
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材料科学
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化学工程
·
吸附技术
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DOI
高影响因子
IEEE SENSORS JOURNAL
·
2024
·Vol. 24
·期 22
·36239-36246
·
IF 4.3
Dong Zhao,Honglie Chen,Kun Yang,Haoyu Wang,Xing Guo,Yang Ge,Xiushan Dong,Shengbo Sang
The application of competitive immunoassay brings many advantages to the detection of trace biomolecules and has the potential to be applied to urine-based clinical practice. However, this type of detection method has strict requirements for secondary antibody incubation processes and analysis equipment, leading to strong demands for convenient, rapid, and inexpensive detection platforms. In this study, a mechanical competitive immunosensor (MCI) was proposed for the detection of human serum albumin (HSA) based on goat anti-rabbit @Fe2O3 magnetic beads with magnetic sensitization. With the doping of Fe2O3, the conversion layer of MCI responds more accurately and rapidly to stress. In addition, goat anti-rabbit conjugated with animated Fe2O3 nanoparticles were introduced as secondary antibodies for signal amplification. Under the synergistic effect of the magnetic force of magnetic beads and the stress caused by the specific binding of antigen and antibody, the deformation of the film was amplified, which can effectively change the conductive pathway formed by doped carbon nanotubes, resulting in a larger output electric signal. Through competitive immunoassay for HSA, a limit of detection (LOD) of 68 ng/mL was achieved, which was an order of magnitude lower than direct detection methods. With high reproducibility and stability, MCI demonstrated effectiveness in the detection of HSA at a clinically significant concentration range (0.1–
$50 \; \mu $
g/mL). Moreover, MCI showed excellent specificity and selectivity, which held promise to offer an alternative tool for clinical diagnosis of urine HSA levels in nephrotic patients.
研究领域:
生物医学工程
·
分析化学
·
纳米技术
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DOI
高影响因子
JOURNAL OF POLYMERS AND THE ENVIRONMENT
·
2024
·
IF 4.7
Chen Jindong,Qiu Hengbin,Djouonkep Lesly Dasilva Wandji,Lv Junxian,Xie Binqiang
Addressing the complex issue of lost circulation in drilling operations is crucial as it increases nonproductive time and costs. Conventional gel plugging materials often exhibit low temperature resistance, poor pumpability, and low pressure-bearing capacity. To overcome the limitations, a self-made polymer (HSA) was initially synthesized and mixed with two crosslinker agents (MBA/PEI) at room temperature to induce a new type of high-temperature-resistant and pressure-resistant polymer gel agent (HSA-G). Investigating the gel strength and plugging capacity, the vertical inverted tube observation, 71-type high-temperature and high-pressure (HTHP) instruments, and high-pressure filter with varying fracture cracks (3–5 mm) were employed. HSA-G demonstrated excellent gelation strength for 4–5 h at 140 °C, while retaining more than 57% of its gel strength after aging at 140 °C for 144 h, which is 3 times higher than the commercially available hydrolyzed polyacrylamide (HPAM + PEI). The excellent performance was attributed to the synergy between PEI-MBA, which induces tight-crosslinked interconnected structure within HSA-G, mitigating fluid losses to only 72 mL compared to 194 mL for HPAM-G, under 8.5 MPa in the HTHP sand bed at 140 °C. In the fracture leakage simulations using a 5 mm crack performed at 6 MPa at room temperature, the filtration loss of HSA-G is 300 mL, almost half that of HPAM-G, showcasing its superior plugging and high-pressure bearing performance. In conclusion, HSA-G has not only demonstrated operational effectiveness in reducing downtime costs and fluid losses but can also temporally replace cement plugging to prevent reservoir contamination in alignment with environmentally friendly practices.
研究领域:
石油工程
·
材料科学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Ziang Zhao,Xinyi Fang,Yanbing Gao,Qiaomei Sun
Flavonoids, diverse plant-derived compounds, show distinct binding behaviors with human serum albumin (HSA), influencing their pharmacokinetics. We examined three flavonoids—chrysin, baicalin, glycitein—using multispectral analysis and molecular simulations. Fluorescence (time-resolved, synchronous, steady-state), circular dichroism, dynamic light scattering and 3D fluorescence confirmed static quenching, ground-state complex formation, increased α-helix content, reduced protein size, and enhanced hydrophobic exposure. Thermodynamic results (ΔG < 0) showed spontaneous, Hydrogen bonds and van der Waals forces driven binding exhibits strong, moderate, and weak affinity, respectively(Kb ≈ 10 3 –10 5 M −1 ); chrysin had the strongest affinity due to optimal 5,7-dihydroxyl substitution, while glycitein's isoflavone structure decreased its affinity. Docking pinpointed Sudlow site III as the binding site. Molecular dynamics revealed increased helicity and decreased HSA flexibility, implying conformational tightening. These findings link flavonoid structural features—hydroxyl pattern and ring connectivity—to binding strength, site specificity, and HSA stability, offering mechanistic insight for designing flavonoid-based therapeutics and improving pharmacokinetic predictions.
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DOI
PubMed
高影响因子
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
·
2023
·
IF 1.3
Zizhen Huang,Jie Wang,Hui Chen,Ruofei Feng,Chunxia Tan
Four organic-polyoxometalate hybrids BR4[SiW12O40] (BR–SiW), BR3[PMo12O40] (BR–PMo), BR4K[EuSiW11O40]·2H2O (BR–EuSiW) and BR6Na3[EuW10O36] (BR–EuW) were fabricated by the polyoxometalates (POMs) anions and berberine cations (BR) noted for the alkaloids in traditional Chinese herbal medicine. These hybrids have been characterized and confirmed. The interaction between hybrids and human serum albumin (HSA) was investigated in a buffer solution (pH 7.4) using ultraviolet–visible light absorption and fluorescence techniques. The classical Stern–Volmer equation was used to analyze the fluorescence quenching at three temperatures (296, 303 and 310 K), and the static quenching mechanism for interaction was proposed. The Thermodynamic parameters, enthalpy, entropy change, and Gibbs free energy of hybrids interacting on HSA were calculated by Scatchard equation. The results indicated that therewas one binding site on the protein and BR–POMs all showed stronger binding force than that of raw materials. Synchronous fluorescence results showed that the binding sites of BR–POMs and HSA were not effectively affected the surrounding microenvironment. The following antibacterial experiments implied that inhibitory effect of hybrids were synergistic effect from organic active ingredient and POMs but the simple combination. All these data were prepared for further research on biology.
研究领域:
生物化学
·
药理学
·
分子生物学
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DOI
高影响因子
危险品化学品经营许可证（带存储）