NANO
·
2017
·
IF 1.025
Xiqing Liu,Xiao Wei,Yeqing Xu,Hongji Li,Kai Lu,Kun Wang,Yongsheng Yan
In this paper, a novel fluorescent nanoswitch based on carbon dots (CDs) was developed for the sensitive and selective determination of Hg 2 + and I − . The CDs were obtained by simple hydrothermal process and had a strong fluorescence emission at 440 nm. The fluorescence of the CDs can be selectively quenched by Hg 2 + ion, and then the I − was added into the system, which can interact with Hg 2 + and recover fluorescence of the CDs. Under optimal conditions, the quenching fluorescence intensity on addition of Hg 2 + has obtained a satisfactory linear relationship covering the linear range of 0–50 μ M with the linear relationship ( R 1 2 = 0 . 9 9 9 1 ), and the limit of detection is 0.047 μ M. The additions of I − could lead to the fluorescence intensity of the solution of CDs and Hg 2 + (50 μ M) recover rapidly, which is linearly related ( R 2 2 = 0 . 9 9 5 7 ) to the concentration of I − in the range from 0 to 70 μ M, the detection limit for I − was calculated to be 0.084 μ M. Moreover, the developed method to detect Hg 2 + and I − was evaluated in real examples, and the fluorescence switching can sensitively and selectively detect Hg 2 + and I − over some potentially interfering ions, the recoveries were up to 97.8–107.0% and 96.7–106.6%, respectively.
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
·
2019
·
IF 2.032
Dan Wang,Yanan Zheng,Xiaosu Fan,Lina Xu,Ting Pang,Ting Liu,Legui Liang,Shan Huang,Qi Xiao
Mercury pollution has always been a huge threat to human health due to its significant toxicity. Thus, it's the continuing goal to obtain new mercury detection techniques that are cost-effective, operational stable, performance efficient, and applicable to the environmental and biological milieus. In this research, the soluble pigment pyocyanin with anti-bacterial and anti-fungal activities, the biosynthesis pathway of which was engineered under the regulation of Hg 2+ -dependent transcriptional activator MerR, was firstly used as the visual detection signal in the whole-cell biosensor. The engineered biosensor displayed optical sensing window and a good linearity for Hg 2+ in the range of 25–1000 nM, and the detection limit could reach as low as 10 nM. It permitted on-site detection of bioavailable Hg 2+ with extraordinary selectivity and could resist the interferences of extra metal ions. What's more, the developed biosensor performed function well in a wide pH range (pH 4–10) as well as the environmental water. By fully imitating and utilizing the biosystems from nature, the engineered colorimetric biosensor has great economic and performance advantages over most chemosensors as well as whole-cell biosensors in the practical application of detecting Hg 2+ in the contaminated aquatic systems.
研究领域:
环境科学
·
微生物学
·
生物技术
✓ 已验证
DOI
PubMed
高影响因子
Analytical Methods
·
2017
·Vol. 9
·期 35
·5121-5126
·
IF 1.9
Li-Juan Wang,Li-Ping Jia,Rong-Na Ma,Wen-Li Jia,Huai-Sheng Wang
A simple colorimetric sensing method for the detection of Hg2+ was developed by combining enzymatic catalysis with DNA-based hybridization chain reactions (HCRs). Firstly, thymine (T)-rich capture DNA (cDNA) was immobilized on a gold electrode via Au–S bonding. In the presence of Hg2+, thymine (T)-rich probe DNA (pDNA) hybridized with cDNA via T–Hg2+–T base pairs. Then the HCRs were realized using pDNA as an initiator and two biotin-labeled hairpin DNAs (H1 and H2) as fuel strands. Finally, numerous avidin-labeled horseradish peroxidase (HRP) enzymes were immobilized on long nicked ds-DNA strands, which can catalyze the H2O2-mediated oxidation of 3,3,5,5-tetramethylbenzidine dihydrochloride hydrate (TMB) to cause a dramatic color change. Under optimal conditions, the absorbance of TMB was linear with the logarithm of Hg2+ concentrations in the range of 1 fM to 1 pM, with a detection limit of 0.33 fM. This strategy exhibited good selectivity and high sensitivity, which might be a potential tool for the practical detection of Hg2+ in environmental monitoring.
研究领域:
分析化学
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环境科学
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生物传感器
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2018
·
IF 4.946
Chong Shen,Bin Peng,Ying Wang,Qin Meng
Milk is often polluted by heavy metal ions due to the growing environment pollution, but few methods have been developed to remove the heavy metal ions. Here a non-toxic sorbent, namely di-acrylated Pluronic P123 (P123-DA) hydrogel, was fabricated for removal of Hg 2+ and Pb 2+ from milk without impairing their nutritive contents. This hydrogel possessed high mechanical stress and maximum adsorption capacity of 35.2 and 53.9 mg/g for Pb 2+ and Hg 2+ . The removal ratio of Pb 2+ and Hg 2+ by P123-DA hydrogel respectively reached 85.3% and 81.9% for the polluted whole milk while was individually 86.3% and 83.8% for the skim milk. Interestingly, the treatment by P123-DA hydrogel didn’t significantly reduce the main nutritive contents in milk. Such hydrogel will be a recyclable, safe and effective tool for reuse of milk that polluted by heavy metal ions.
研究领域:
食品科学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2014
·
IF 0.7
Yue XU,Chun-Jing TANG,Hong HUANG,Chao-Qun SUN,Ya-Kun ZHANG,Qun-Feng YE,Ai-Jun WANG
Fluorescent carbon quantum dots (CQDs) were synthesized by one-step hydrothermal treatment with apple juice as raw material. The result indicated that the fluorescence could be quenched by Hg 2+ with high specificity. Based on this phenomenon, a selective and sensitive sensor was constructed for the detection of Hg 2+ in phosphate solutions (pH 7.0). The fluorescence intensity showed linear responses with Hg 2+ concentration ranging from 5.0 to 100.0 nM and 1.0 to 50.0 μM, with the detection limit of 2.3 nM ( S / N = 3). The as-fabricated sensor was further extended for the determination of Hg 2+ in real water samples.
研究领域:
分析化学
·
环境科学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Ruyan Xie,Yaoyao Qu,Mingyu Tang,Jingqiang Zhao,Sophie Chua,Tingting Li,Fang Zhang,Andrew E. H. Wheatley,Fang Chai
The dual functional detection and removal of heavy ion metals by carbon dots has become an urgent matter of concern. Here, a unique fluorescent carbon dot-magnetic nanocomposite (Fe 3 O 4 /CDs) was prepared by hydrothermal methods for sensitive detection of Hg 2+ . The Fe 3 O 4 /CDs serve as fluorescent probes with higher selectivity and sensitivity for Hg 2+ , with the lowest detectable limit of 0.3 nM. Hg 2+ statically quenched the blue emission of Fe 3 O 4 /CDs, which can be restored in the presence of saturated EDTA solution. The utilization of Fe 3 O 4 /CDs was fulfilled by recovering their emission conveniently. The recovery of Hg 2+ in Chagan Lake water, tap water and drinks was calculated at 96.5 ~ 108.8%, which demonstrates the feasibility of the Fe 3 O 4 /CDs sensing system in natural samples. Notably, the Fe 3 O 4 /CDs can drive the effective removal of Hg 2+ from samples, which is of outstanding significance as a promising probe in environmental monitoring.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2024
·
IF 5.3
Xiaolu Xing,Ying Zhang,Yan Tan,Tianhao Hu,Ziqiu Chen,Bowen Liu
Surface enhanced Raman spectroscopy (SERS) offers ultrasensitive and label-free detection of specific molecules with broad applications in chemistry, life sciences, security, environmental sciences, and sensors. Among environmental contaminants, Hg2+ poses a significant threat due to its high toxicity, necessitating precise and high-throughput detection methods. Here, we introduce a novel SERS chip that integrates a multiwell plate with a plasmonic array, boasting strong electromagnetic enhancement and exceptional uniformity. Through surface modification of the chip with I–, we achieve high throughput, selectivity, and low limit of detection (LOD) for Raman-inactive Hg2+. Notably, the chip serves as a convenient platform for real-time Hg2+ detection and offers versatility for various surface modifications, paving the way for a next-generation quantitative and high-throughput sensor for a Raman-inactive analyte.
研究领域:
环境科学
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分析化学
·
生物传感
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FLUORESCENCE
·
2023
·
IF 2.7
Zhang Cheng-lu,Liu Chang,Nie Shi-ru,Zhang Yang,Guo Jing-hao,Li Xiang-ling,Liu Cui
For the efficient detection of Hg 2+ and ClO − , a double-analyte-responsive fluorescent probe PTB was successfully synthesized by combining N-butyl-3-formyl phenothiazine with hydrazine benzothiazole, and designing a specific reaction site for recognizing two analytes (Hg 2+ and ClO − ) in a compound. It was shown that probe PTB successfully formed a stable complex with Hg 2+ in the coordination ratio of 2:1 by using the strong sulfur affinity of Hg 2+ , which resulted in a remarkable "turn-off" effect, with a quenching efficiency of 92.5% and four reversible cycles of Hg 2+ fluorescence detection. For the fluorescence detection of Hg 2+ , the response time is fast (≤ 2 min) and the detection limit is low (7.8 nM), showing extremely high sensitivity, and the performance is obviously better than that of the reported fluorescent probes for detecting Hg 2+ . In particular, probe PTB has low toxicity and good biocompatibility, and has been successfully used for imaging of Hg 2+ in living cells. Moreover, probe PTB uses thioether bond and carbon-nitrogen double bond as reaction sites to detect ClO − , which has large Stokes Shift (149 nm), good selectivity, high quenching efficiency (96.5%) and fast time response (about 10 s), and successfully detects ClO − in actual water samples. Graphical The dual functional fluorescent probe PTB is sensitive for Hg 2+ and ClO − . It has been successfully used for making pH fluorescent test paper and imaging detection of exogenous Hg 2+ in VSMC cells with low toxicity.
研究领域:
分析化学
·
生物医学
✓ 已验证
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2019
·
IF 2.931
Rong-Mei Kong,Lin Ma,Xue Han,Chunran Ma,Fengli Qu,Lian Xia
G-triplexes have been reported recently with the similar function to G-quadruplex that can combine with thioflavin T (ThT) and emit strong fluorescence but easier to be controlled and excited. In this work, we report an Hg 2+ -mediated stabilization of G-triplex based functional molecular beacon (G3TMB) sensing system for the label-free detection of Hg 2+ , reduced glutathione (GSH), and glutathione reductase (GR) activity. In the presence of Hg 2+ , the extended G-triplex sequence containing the “T” bases can form a stable hairpin structure due to the strong interactions of “T-Hg 2+ -T”, resulting in the locking of G-tracts in the stem of the G3TMB effectively. However, the hairpin structure of the G3TMB can be opened by the introduction of GSH through the stronger “GSH-Hg 2+ ” interaction. Therefore, by employing the fact that GR can catalyze the reduction of oxidized glutathione (GSSG) into GSH, this concept can be applied to fluorescence “off-on” detection of GR activity, with a linear range of 0.02–30 mU/mL and detection limit of 0.01 mU/mL. This work may expand a new perspective of G-triplex based functional molecular beacon as the label-free fluorescent probes in the detection of small biomolecule and enzyme activity .
研究领域:
生物化学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
Foods
·
2023
·Vol. 12
·期 5
·1085
·
IF 5.2
Ziwen Zhang,Ran Han,Sixuan Chen,Feilin Zheng,Xinmiao Ma,Mingfei Pan,Shuo Wang
In this study, a rapid fluorescent and colorimetric dual-mode detection strategy for Hg2+in seafoods was developed based on the cyclic binding of the organic fluorescent dye rhodamine 6G hydrazide (R6GH) to Hg2+. The luminescence properties of the fluorescent R6GH probe in different systems were investigated in detail. Based on the UV and fluorescence spectra, it was determined that the R6GH has good fluorescence intensity in acetonitrile and good selective recognition of Hg2+. Under optimal conditions, the R6GH fluorescent probe showed a good linear response to Hg2+(R2= 0.9888) in the range of 0–5 μM with a low detection limit of 2.5 × 10−2μM (S/N = 3). A paper-based sensing strategy based on fluorescence and colorimetric analysis was developed for the visualization and semiquantitative analysis of Hg2+in seafoods. The LAB values of the paper-based sensor impregnated with the R6GH probe solution showed good linearity (R2= 0.9875) with Hg2+concentration in the range of 0–50 μM, which means that the sensing paper can be combined with smart devices to provide reliable and efficient Hg2+detection.Keywords:Hg2+;R6GH;dual mode;fluorescence;visualization
研究领域:
分析化学
·
环境科学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MATERIALS & DESIGN
·
2016
·
IF 3.997
Xinjian Cheng,Yun Yu,Yiru Jia,Lian Duan
Three small molecular boron dipyrromethene (BODIPY) derivatives (B3a, B3b and B3c) were well-designed and synthesized at first, and then were incorporated into polyurethane to obtain macromolecular fluorescent sensors. These small molecular BODIPY derivatives exhibited high sensitivity and selectivity to Hg 2 + , Cr 3 + and Fe 3 + ions. The colors of these chemicals changed rapidly upon the addition of Hg 2 + , Cr 3 + and Fe 3 + ions. In addition, their fluorescence peaks shifted and was enhanced as well. B3a, B3b and B3c still remained the sensing properties when being introduced to polyurethane (PU). The functionalized PU films not only showed color change and fluorescence shift for detecting Hg 2 + , Cr 3 + and Fe 3 + ions as their small molecular counterparts, but also triggered a new emission peak in 500–600 nm. Besides, the functionalized PU film (PU-B3a as a sample) could absorb Hg 2 + ions more than 84 mg/g in unit time and volume. It suggested that as long as the structure of the small molecular sensor was remained, the fluorescent ability remained even it is introduced to the macromolecular chains. In addition, Glass transition temperature (T g ) of the PU films changed when B3a, B3b and B3c were introduced.
研究领域:
材料科学
·
环境科学
·
化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FLUORESCENCE
·
2025
·
IF 3.1
Han Bo-Ming,Zhang Hao-Rui,Gao Wen-Jie,He Xi-Wen,Li Wen-You,Zhang Yu-Kui
In this work, a dual-emission material based on lanthanide metals was synthesized and used as a ratio fluorescence sensor to respond to Hg 2+ and H 2 O 2 , respectively. Tb-BDC-NH 2 as a bimetal MOFs had double ligands and exhibited double fluorescence emission peaks at 550 nm and 450 nm. The two emission peaks of Tb-BDC-NH 2 can be quenched by Hg 2+ and H 2 O 2 respectively, forming a built-in calibration signal for ratio fluorescence detection. Based on the different detection conditions of the Hg 2+ and H 2 O 2 , the simultaneous detection of the two detection substances can be achieved with simple operation. This detection system had good stability and anti-interference ability. Under optimal conditions, the detection limit for Hg 2+ detection was 0.2 µM. Meanwhile, for the detection of H 2 O 2 , the detection limit was 11 µM and large linear ranges were obtained. Through actual sample testing, it had been proven that the ratio fluorescence sensor based on Tb-BDC-NH 2 had good application prospects. And the work also provided a new idea for double substances detection.
研究领域:
分析化学
·
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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