ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Zhiwei Qie,Qiqi Liu,Wenliang Yan,Zichen Gao,Wu Meng,Rui Xiao,Shengqi Wang
A universal and ultrasensitive immunochromatographic assay (ICA) was established using antigen as a bifunctional element and antialbumin antibody in a test line. Preincubation was introduced for competitive recognition. After optimization, the linear detection of aflatoxin M1 (AFM1) with quantum dot bead (QB)-based ICA (QB-ICA) sensor ranged from 10 to 52 pg mL–1, with a 50% inhibitory concentration (IC50) of 23 pg mL–1, which was nearly 49.6-fold lower than those of ICA on a traditional structure with traditional pretreatment (IC50 = 1.10 ng mL–1) and 10-fold lower than those of ICA on a traditional structure with acid aid pretreatment (IC50 = 0.25 ng mL–1). The limit of detection (LOD) for AFM1 was 16 pg mL–1 in milk, which was approximately 16.3-fold times higher than those of ICA on a traditional structure with traditional pretreatment and 6.3-fold higher than those of ICA on a traditional structure with acid aid pretreatment. The LOD improved by 20-fold by using the proposed structure compared to that of conventional enzyme-linked immunosorbent assay (ELISA) for AFM1-spiked milk samples (IC10 = 0.12 ng mL–1). The performance and practicability of the established QB-ICA sensor were validated with a commercial ELISA kit. To evaluate universality, we successfully detected chloramphenicol, with IC50 of 0.42 ng mL–1. Given its high sensitivity and universality, the proposed QB-ICA can be used as an alternative for rapid, sensitive, and universal quantitative detection of all small-molecule analytes.
研究领域:
分析化学
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食品安全
·
生物技术
·
免疫学
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产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2021
·
IF 7.086
Ze-Jun Wang,Qiao-Feng Zheng,Shu-Shen Liu,Peng Huang,Ting-Ting Ding,Ya-Qian Xu
At present, the toxicity prediction of mixtures mainly focuses on the concentration addition (CA) and independent action (IA) based on individual toxicants to predict the toxicity of multicomponent mixtures. This process of predicting the toxicity of multicomponent mixtures based on single substances or low component mixtures is called down-to-top method in this study. However, due to the particularity of some toxicants, we have to use the top-to-down idea to obtain or eliminate the toxicity of some components from mixtures. For example, the toxicity of toxicants is obtained from the toxicity of a mixture with, especially toxic, cosolvent added. In the study, two top-to-down methods, the inverse CA (ICA) and inverse IA (IIA) models, were proposed to eliminate the effects of a certain component from multicomponent mixtures. Furthermore, taking the eight binary mixtures consisting of different shapes of cosolvents (isopropyl alcohol (IPA) having hormesis and dimethyl sulfoxide (DMSO)) and toxicants (two ionic liquids and two pesticides) as an example, combined with the interaction evaluated by CA and IA model, the influence of different shapes of components on top-to-down toxicity prediction was explored. The results showed that cosolvent IPA having hormesis may cause unpredictable effects, even at low concentrations, and should be used with caution. For DMSO, most of the toxicant's toxicity obtained by ICA and IIA models were almost in accordance with those observed experimentally, which showed that ICA and IIA could effectively eliminate the effects of cosolvent, even if toxic cosolvent, from the mixture. Ultimately, a frame of cosolvent use and toxicity correction for the hydrophobic toxicant were suggested based on the top-to-down toxicity prediction method. The proposed methods improve the existing framework of mixture toxicity prediction and provide a new idea for mixture toxicity evaluation and risk assessment.
研究领域:
环境毒理学
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风险评估
·
化学毒理学
·
药学
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2024
·
IF 6.1
Wan Huang,Song Zhang,Li Luo,Yalong Pan,Lijun Han,Yao Yu
Multimodal therapy based on nanozyme is expected to become a novel option for tumor treatment. However, the catalytic efficiency of nanozyme and the hypoxia microenvironment of tumors limit the therapeutic effect of nanozyme. Here in this article, we screened a small molecule midazole-2-carboxaldehyde (ICA) to preparation ZIF-90 and embedded gold and platinum nanoparticles to obtain ZAAP. ZAAP possessed a multi-enzymatic cascade of catalytic process including greatly enhanced peroxidase activity via "bionic" catalytic microenvironment (enhanced 23-fold), catalase and glucose oxidase activities, resulting in glucose decomposition to continuously supply H2O2, peroxidases for the catabolism of H2O2 to generate ROS and peroxidase-induced oxygen generation for continuous oxidation of glucose. All the above process built a catalysis cycle which greatly promotes the generation of ROS and oxygen as well as the consumption of glucose, leading to the chemical dynamic therapy function and alleviating the tumor hypoxia. In addition to the photothermal effect of ZAAP, a synergistic treatment of chemical dynamic/ photothermal/ starvation therapy can be achieved and the tumor inhibition rate reached 96.4% within 2 weeks, indicating that ZAAP show great potential in nanozyme based synergistic multimodal tumor treatment.
研究领域:
纳米技术
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癌症治疗
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生物医学工程
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材料科学
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产品关联
DOI
PubMed
高影响因子
VIBRATIONAL SPECTROSCOPY
·
2021
·
IF 2.507
Lei Zhang,Yingshan Chen,Qin Zhu,Wenjin Ji,Suqing Zhao
Bisphenol A (BPA) is a typical endocrine-disrupting chemical (EDC), which has been banned for food packing and baby bottle material due to its potential endocrine harm to human body. In this paper, an easy and rapid immunochromatographic assay (ICA) combined with the technology of surface-enhanced Raman spectroscopy (SERS) was fabricated to improve the sensitivity and rapidity for quantitative determination of BPA. After 10 min of a quick chromatographic process, the specific Raman signal on T line of the test strip was measured. Compared with the traditional ICA, the SERS-ICA realized quantitative test and showed the limit of detection (LOD) was 0.1 ng/mL, getting approximately 300-fold lower. The method displayed high specificity and precision with RSD of 4.1%, and the test strip indicated favorable stability for two weeks without loss of activity. For river, tap and commercial drinking water sample, the SERS-ICA results were confirmed by HPLC, and the recoveries of spiked BPA were from 90.80-117.80%. It was shown that the proposed method takes advantages both of SERS and ICA, exhibiting high sensitivity, precision, stability, quantification and rapidity for field test, expecting a widely useful method for various small molecules in food safety, medical diagnosis and environmental samples.
研究领域:
分析化学
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环境科学
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食品安全
·
医学诊断
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产品关联
DOI
高影响因子
AICHE JOURNAL
·
2019
·Vol. 65
·期 8
·e16620
·
IF 3.463
Xiaoxu Zhao,Hao Zhang,Sheng Xu,Yan Wang
In this study, a novel covalent-assisted seeding method is developed to fabricate well-intergrown ZIF-8 membrane on polyimide (PI) substrate with imidazole-2-carboxaldehyde (ICA) as the covalent agent between ZIF-8 layer and PI substrate. Compared to ethylenediamine-crosslinked PI membrane and ZIF-8/PI membrane synthesized without covalent-assisted seeding method, ZIF-8/PI membrane synthesized with ICA covalent-assisted seeding method shows improved pervaporation performance for isopropanol dehydration. Subsequent surface coating with polydimethylsiloxane or polydopamine is applied to mitigate the grain boundary of ZIF-8/PI membrane, which results in a further performance enhancement. The morphologies and physicochemical properties of the resultant membranes are characterized by scanning electron microscope, attenuated total reflectance-Fourier transform infrared, X-ray diffraction, and water contact angle. The effect of operation temperature on the separation performance is also carried out. This work demonstrates the novel covalent-assisted seeding method for the preparation of ZIF-8 membrane with high-performance, which may hold a great potential for various ZIF membranes separation applications.
研究领域:
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Dan Zhang,Yu Fan,Gaojie Li,Wei Du,Runlong Li,Yiming Liu,Zhixuan Cheng,Jiaqiang Xu
A series of zeolitic imidazolate frameworks (ZIFs) were synthesized by a facile and environmental friendly biomimetic method. The textural properties of the ZIFs were characterized by PXRD, FESEM, TEM and FT-IR spectra. These results showed ZIF 90 having typical rhombic dodecahedron crystals with a diameter of 800–1200 nm. Furthermore, the presence of rich aldehyde groups is confirmed through chemical analysis. Gas-sensing tests of quartz crystal microbalance (QCM) transducers based on ZIFs were investigated. The results showed that ZIF-90 based sensors exhibited excellent sensing performance towards low concentration of acetone vapor, such as high sensitivity (the frequency shift was 95 Hz @ 1 ppm), fast response (the response/recovery time was 12 s/17 s, respectively) and good selectivity. On the basis of temperature-varying experimental method, a moderate adsorption enthalpy (the value of ΔH θ was −57.93 kJ/mol) was obtained, indicating the existence of weak and reversible chemisorption between ZIF-90 and acetone molecules. The value of adsorption enthalpy (ΔH = −60.10 kJ/mol) obtained from Density functional theory is in close agreement with the experimental results.
研究领域:
材料科学
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化学工程
·
传感器技术
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2020
·
IF 10.652
Qiufang Chen,Bin Deng,Qing Luo,Guanbin Song
Cancer stem cells (CSCs), which are resistant to the traditional therapies, have been considered to account for the development, metastasis, and relapse of various malignant tumors, and therefore, must be eliminated to cure cancer. However, they can reside in deep tumor regions away from the blood vessels, and are typically inaccessible owing to the low delivery efficiency and limited tumor penetrating ability of drug carriers. To overcome these problems, we designed a tumor-penetrating peptide (tLyP-1)-conjugated ZIF-90 nanosystem loaded with doxorubicin (DOX) and N-[N-(3,5-difluorophenacetyl)-1-alanyl]-S-phenylglycine t-butyl ester (DAPT) to penetrate and eradicate differentiated cancer cells and CSCs simultaneously. The nanosystem exhibited beneficial biocompatibility, enhanced tissue penetration, high blood circulation stability, and pH-responsive drug release for favoring cancer therapy. It was found that the nanosystem could kill both cancer cells and CSCs in vitro . The in vivo results demonstrated that the ZIF-90 nanosystem can effectively accumulate in tumor tissues after long blood circulation and flexibly permeate throughout the tumor tissues. In the HCCLM3 xenograft model, the ZIF-90 nanosystem presented high-efficiency tumor suppression and drastically eradicated the CSCs in the tumor tissues with low systemic toxicity. Overall, the deep tumor-penetrating nanosystem exhibits outstanding potential for improving curative effect.
研究领域:
肿瘤学
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药学
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纳米技术
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生物医学工程
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产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2021
·
IF 4.539
Chenxi Yu,Wenyu Zhu,Zhaozhan He,Jin Xu,Fengyan Fang,Ziang Gao,Wenli Ding,Yixiang Wang,Jing Wang,Junqiao Wang,Ao Huang,Aohua Cheng,Yanhui Wei,Shiyun Ai
Poly(lactic-co-glycolic acid) (PLGA) based microparticles have been widely applied in drug delivery system, but pristine PLGA particles suffers from low efficiency in release and non-targeted effect for drug delivery system. Inspired by cancer cells environment, we have fabricated an ATP-triggered delivery system based on porous PLGA microspheres covering drug-loaded ZIF-90. The system contains controlled doxorubicin releasing because ZIF-90 nanocrystals are easily collapsed under exposing to high-concentration ATP environment. In addition, [email protected] microparticles also perform low toxicity and 2.37 % hemolysis, and improved fluorescence intensity of doxorubicin by loading riboflavin sodium phosphate benefited from their partial Förster resonance energy transfer. This novel ATP-triggered drug delivery system based on porous PLGA particles could be widely utilized to design effective drug release biomaterials for gastric and lung cancer.
研究领域:
药剂学
·
生物材料
·
癌症研究
·
药物传递系统
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产品关联
DOI
高影响因子
JOURNAL OF FLUORESCENCE
·
2016
·Vol. 27
·期 1
·227-233
·
IF 1.601
Yan Dan,He Yu,Ge Yili,Song Gongwu
A novel fluorescence method for sensitive and selective detection of phosphate was developed based on near infrared emission Ag 2 S QDs/ Metal − Organic Shell Composite via the deposition of metal-organic (zinc-nitrogen) coordination shell around Ag 2 S QDs . Under optimal conditions described, the fluorescence intensity of the composite was decreased at 685 nm in the presence of phosphate, which was linearly related to the concentration of phosphate in the range of 0. 7 to 4.2 μM and 11.2 to 88.2 μM with the relative correlation coefficient of R 2 = 0.998 and 0.987 respectively and detection limit as low as 6 nM. In addition, the proposed method was successfully utilized in serum samples, tap water and Yangtze River water samples with the recoveries ranged from 94.76 to 100.86 %, which presaged more opportunities for application in related bioassay and water sample researches.
研究领域:
分析化学
·
环境科学
·
生物医学
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产品关联
DOI
PubMed
高影响因子
International Journal of Biological Sciences
·
2019
·
IF 4.067
Haotian Li,Xinran Zhang,Xi Qi,Xu Zhu,Liming Cheng
Endoplasmic reticulum (ER) stress-induced neuronal apoptosis is a crucial pathological process of spinal cord injury (SCI). In our previous study, icariin (ICA) showed neuroprotective effects in SCI. However, the relationships between ER stress and ICA in SCI are unclear yet. Therefore, whether ICA could ameliorate SCI via attenuating ER stress was investigated in vitro and in vivo . Adult mice were established SCI model and received vehicle solution or ICA by gavage once per day in vivo . The primary cultured cells were treated with or without thapsigargin (TG), ICA or LY294002 to induce ER stress in vitro . Motor dysfunction, neuronal apoptosis, tissue damage and inhibition of PI3K/AKT pathway were induced by ER stress after SCI. But ICA administration significantly enhanced motor recovery and protected spinal cord tissues against infraction and hemorrhage, etc. post injury. Meanwhile, the expression of ER stress markers ATF6, IRE1α, GRP78, XBP1 and eIF2α was decreased, while the level of p-AKT/AKT was increased by ICA. Furthermore, ICA significantly inhibited the expression of ER stress apoptotic proteins caspase-12, CHOP, Bax/Bcl-2, caspase-9 and caspase-3. Moreover, immunofluorescence double staining indicated that ICA reduced GRP78, CHOP and TUNEL positive neurons following SCI. However, this beneficial effect of ICA was abolished by PI3K/AKT inhibitor LY294002 in vitro . Finally, ICA preserved the ultra-structure of ER by transmission electron microscope histologically. This study suggested that the neuroprotective effect of ICA on motor recovery and neuronal survival was related to attenuating ER stress via PI3K/AKT signaling pathway after SCI.
研究领域:
神经科学
·
药理学
·
分子生物学
·
病理生理学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2020
·Vol. 10
·期 20
·12105-12112
·
IF 3.119
Xiaofeng Shen,Pengfei Yu,Hua Chen,Jiangping Wang,Binjie Lu,Xuefeng Cai,Chun Gu,Guoqiang Liang,Donglin Hao,Qihan Ma,Yuwei Li
The treatment of bone defects caused by various reasons is still a major problem in orthopedic clinical work. Many studies on osteogenic implant materials have used various biologically active factors such as osteogenic inducers, but these biologically active factors have various side effects. Therefore, in this study, silk fibroin (SF) was used as a scaffold material, mesoporous bioactive glass nanoparticles (MBGNs) as a sustained release carrier, and the traditional Chinese drug icariin (ICA) was loaded to promote bone formation. The experiments in this study have proven that SF/MBGNs-ICA scaffolds can successfully load and release ICA for a long time, and the sustained-release ICA can promote the proliferation and differentiation of BMSCs for a long time. This controlled-release ICA organic/inorganic two-component scaffold material is expected to become a new bone grafting solution.
研究领域:
生物材料
·
组织工程
·
骨科
·
药剂学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CORROSION SCIENCE
·
2020
·
IF 6.479
Xiaobang Liu,Ting Yue,Kai Qi,Bao Yu Xia,Zhenyu Chen,Yubing Qiu,Xingpeng Guo
Metal-organic framework (MOF) membranes as anticorrosion coatings are fabricated onto diverse metal surfaces via a versatile approach based on utilization of polydopamine layer, whose strong adhesion and metal-chelating capabilities promote heterogeneous nucleation and growth of MOF membranes. The anticorrosion property of MOF membranes has been achieved owing to characters of well-intergrown crystals, compact structure, hydrophobicity and effective rejection of corrosive ions of MOF membranes, and would be affected by MOF crystal size, membrane thickness and metal substrate. This study may shed some valuable light on the construction of MOF-based protective coatings as qualified and important candidates for high-performance anticorrosion applications.
研究领域:
材料科学
·
表面工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）