MICROBIOLOGY AND IMMUNOLOGY
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2017
·Vol. 62
·期 3
·187-194
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IF 1.706
Cenrong Zhang,Wei Xu,Jian Chen,Ran Guan,Shicheng Bi,Haibo Jin,Xuemei Cui,Fushan Shi,Songhua Hu
In the present study, the adjuvant effect of soybean oil containing ginseng root saponins (SO-GS-R) on the immune response to foot-and-mouth disease vaccine (FMDV) in mice was investigated. When immunized with FMDV antigen emulsified in an SO-GS-R formulation, mice generated remarkably higher serum antibody and cytokine responses than mice immunized with FMDV antigen alone. To elucidate the mechanisms underlying the adjuvant effect of SO-GS-R, we measured cytokines in serum and muscle tissue after intramuscular injection of SO-GS-R. The results showed that injection of SO-GS-R significantly increased the levels of IL-1β, IL-5, IL-6, G-CSF, KC, MCP-1, MIP-1α, and MIP-1β in both serum and muscle. These results suggested that SO-GS-R recruits neutrophils, eosinophils, T cells and macrophages, causing immune cell recruitment at the injection site, driving antigen-presenting cells to actively participate in the onset of immunity, and amplifying the immune responses. Considering its adjuvant activity and plant-derived properties, SO-GS-R should be further studied for its adjuvant effect on vaccines used in food animals.
研究领域:
免疫学
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兽医学
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药理学
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DOI
PubMed
高影响因子
Scientific Reports
·
2024
·Vol. 14
·期 1
·1-13
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IF 3.8
Bu Daolu,Yang Hu,Zhang Haibo,Wang Liang,Wang Jiao,Liao Jie
The present study investigates the efficacy of newly developed Zr-based metal–organic frameworks, specifically MIP-206, and its amine-modified counterpart, MIP-206-NH 2 , for the re-covery of antimony (Sb) from both synthetic and actual mining wastewater. Batch method studies were employed to examine the effect of waste media pH, Sb concentration, process kinetics, and the performance of the regeneration solution. MIP-206-NH 2 exhibited impressive separation capabilities, achieving 102.18 mg/g and 63.23 mg/g for Sb (V) and Sb (III), respectively. In contrast, the pristine MIP-206 reached maximum values of 26.26 mg/g for Sb (V) and 16.95 mg/g for Sb (III). The separation process was well-described by the Langmuir equation, and the kinetics followed the pseudo-second-order model. Although the amine modification resulted in a decrease in the surface area of MIP-206 from 1345.21 to 1169.86 m 2 /g, SEM and XRD analyses confirmed that the structural integrity of MIP-206-NH 2 remained unchanged. In terms of reusability, MIP-206-NH 2 maintained up to 90% of its separation performance over 9 cycles, while MIP-206 demonstrated effectiveness for 7 cycles. The regeneration solution exhibited a capacity of approximately 0.63 mol/L for Sb (V) and 0.71 mol/L for Sb (III). Furthermore, MIP-206 and MIP-206-NH 2 demonstrated successful application in selectively separating Sb from real mining wastewater.
研究领域:
环境科学
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材料科学
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DOI
PubMed
高影响因子
Surfaces and Interfaces
·
2024
·
IF 5.7
Jing Li,Zhiyong Yao,Xiaomei Liu,Cailing Yang,Pengbo Liu,Panpan Liu,Weiqiang Wang,Haonian Guo,Guoying Huang,Xinlu Jin,Ziqiang Lei
Atmospheric water harvesting (AWH) emerges as a promising technology to address the challenge of water scarcity by efficiently harnessing the abundant water present in the atmosphere. This study focuses on enhancing the performance of zirconium-based metal-organic frameworks (MOFs), particularly MIP-202, which is recognised for its facile synthesis and eco-friendly characteristics. Through post-synthesis modifications, MIP-202 was tailored for application as a cross-linking agent in the fabrication of hygroscopic polymer material (MPA) via free radical polymerisation alongside acrylic acid radicals. Our investigation centers on assessing the atmospheric water harvesting (AWH) capacity of MPA across varying humidity levels and its efficacy in liquid water storage. Experimental findings demonstrate MPA's broad adsorption spectrum, from 30% to 90% relative humidity, with adsorption capacities ranging from 44.6 mg/g to 1372.4 mg/g. At a solar intensity of 1 kW/m 2 (equivalent to 1 sun), the desorption rate reaches 0.39 kg/m 2 /h. Following ten cycles of adsorption-desorption experiments, MPA maintains a stable adsorption capacity of 824 mg/g, retaining 95.7% of the initial adsorption capacity. The straightforward synthesis process of MPA, coupled with its enhanced water vapour trapping capability and consistent cyclic stability, positions it favourably as a promising material for atmospheric water harvesting applications.
研究领域:
材料科学
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环境科学
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化学工程
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DOI
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 34
·27034-27042
·
IF 3.84
Haicheng Liu,Wei Chen
A magnetic molecularly imprinted polymer (MSEP@MIP) adsorbent was prepared by using magnetic Fe3O4-modified sepiolite (MSEP) particles as magnetic carrier for the efficient removal of herbicide atrazine from aqueous solution. The composition, thermal stability, chemical structure, specific surface area, morphology, and magnetic properties of MSEP@MIP adsorbent were characterized by X-ray diffraction (XRD), thermo-gravimetric analysis (TGA), Fourier transform infrared spectroscopy (FTIR), Brunauer–Emmett–Teller (BET) method, scanning electron microscopy (SEM) and vibrating sample magnetometry (VSM). Absorption isothermal and kinetics experiments were employed to investigate the adsorption capacity of atrazine onto MSEP@MIP. The prepared MSEP@MIP adsorbent was mesoporous. Compared with magnetic non-imprinted polymer (MSEP@NIP) and MSEP, MSEP@MIP showed greater removal efficiency for atrazine (about 91.3% for an initial concentration of 0.1 mg L−1). Kinetic studies demonstrated that the adsorption process onto MSEP@MIP followed a pseudo-second-order rate equation. Isotherm studies indicated that the atrazine adsorption onto MSEP@MIP was a monolayer molecular adsorption with a maximum adsorption amount of 69.53 mg g−1. Scatchard analysis showed that there are two different kinds of binding sites in MSEP@MIP. Furthermore, the thermodynamics parameters indicated that the reaction between MSEP@MIP and atrazine was physical, exothermic, and nonspontaneous in nature.
研究领域:
环境科学
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材料科学
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DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE
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2021
·Vol. 56
·期 22
·12803-12813
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IF 4.22
Ali M. R.,Bacchu M. S.,Al-Mamun M. R.,Rahman M. M.,Ahommed M. S.,Aly M. Aly Saad,Khan M. Z. H.
An electrochemical sensor based on molecular imprinted polymer (MIP) to detect ceftizoxime (CFX) with high sensitivity and selectivity is demonstrated. MIP was synthesized by electropolymerization of poly-cysteine (P-Cys) on a multi-walled carbon nanotube (MWCNT) modified glassy carbon electrode (GCE). A targeted drug was used as a template molecule during the polymerization process. The bare GCE was coated with a layer of MWCNT before the synthesis of MIP to improve the sensor sensitivity. Experimental parameters such as polymerization conditions, the influence of pH, molar ratio of the template molecules and the monomer molecules were all optimized. The peak potential exhibited linearity with CFX concentration in the ranges of 1 \(\times 10^{ - 9}\) –1× \(10^{ - 7}\) molL −1 ( R 2 = 0.9904) and 2× \(10^{ - 7}\) –1× \(10^{ - 4}\) molL −1 ( R 2 = 0.9949). The LOD of the MIP sensor was found to be \(1 \times 10^{ - 10}\) molL −1 under optimal conditions using a differential pulse voltammetry (DPV). The proposed sensor was tested on real samples, and good recovery results were obtained.
研究领域:
分析化学
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药物分析
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生物传感技术
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DOI
高影响因子
Analytical Methods
·
2019
·Vol. 11
·期 35
·4501-4510
·
IF 2.378
Pu Wu,Qiuzheng Du,Yiyang Sun,Zhonghong Li,Hua He
Lincomycin (LCM) has low ultraviolet (UV) absorption and thus, its determination requires complex instrumentation. Herein, a novel fluorometric probe based on an europium metal–organic framework coated with molecularly imprinted polymers (Eu(BTC)-MIP) was fabricated for the selective determination of LCM. First, highly luminescent Eu(BTC) was synthesized at room temperature. Then, through a simple co-polymerization process, MIPs were formed on the Eu(BTC) surface in the presence of LCM as template molecules to obtain Eu(BTC)-MIP. Eu(BTC) acted as a supporting substrate and antenna, and the MIP layer provided specific binding sites for LCM. Due to the advantages of both Eu(BTC) and MIPs, the probe had a long emission lifetime, strong fluorescence and specific recognition. Under the optimized conditions, the probe achieved the selective determination of LCM in the concentration range of 10 to 100 μg L−1 with a detection limit (LOD) of 7.18 μg L−1 (S/N = 3). This method is attractive because of its low cost, good selectivity, ready availability and simple manipulation without the need for complex instrumentation. Furthermore, the probe was successfully employed to detect LCM in egg samples with a recovery in the range of 88.02% to 97.29% and a relative standard deviation (RSD) of less than 3.46%. The satisfactory results demonstrate that the proposed probe based on Eu(BTC)-MIP can be used for the quantitative analysis of LCM and exhibits great potential for LCM determination in food samples. Also this study provides a new perspective for the determination of weak ultraviolet absorption or non-fluorescent substances without conjugated groups.
研究领域:
分析化学
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食品科学
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材料科学
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DOI
高影响因子
FOOD AND AGRICULTURAL IMMUNOLOGY
·
2020
·
IF 0
Meiguo Xin,Lin Zeng,Di Ran,Xiangmei Chen,Yang Xu,Daoxuan Shi,Yonghong He,Suyi Zhong
A label-free and rapid detection of cooked rat meat-based molecular-imprinted polymer (MIP) conjugated with quantum weak measurement system (QWM) has been developed. Cooked rat protein-specific MIP was prepared by a modified reverse-phase microemulsion method and coated on the internal surface of measuring channels assembled into Mach-Zrhnder interferometer of an optical weak measurement system. The measurement is based on the optical phase shift due to the refractive index change, which is induced by the specific capture of rat protein at its MIP sites. The results can be characterized and calculated by the central wavelength shift of output spectra through weak value amplification. This MIP-WQM system was demonstrated with cooked rat protein showing linear dynamic range from 0 to 20 µg/L. The limit of detection is calculated as 0.1 µg/L for cooked rat meat protein. The system also showed high specificity to cooked rat meat protein, compared to cooked pork meat proteins.
研究领域:
食品科学
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光学检测
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生物技术
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DOI
TALANTA
·
2023
·
IF 6.1
Yulin Lu,Qunshan Liu,Bangguo Fu,Pan Li,Weiping Xu
Early diagnosis of colorectal cancer can significantly improve the overall survival rate of patients, thus selective and sensitive detection of biomarkers in serum samples is vital for early detection and dynamic monitoring of cancer. Nucleoside diphosphate kinase NM23-H2 (NDKB) is an important biomarker and therapeutic target for the diagnosis of colorectal cancer (CRC). Here, a label-free and ultrasensitive biosensor for NDKB protein markers is presented for the first time, combining the characteristic capture selectivity of molecularly imprinted polymers (MIPs) and the ultrasensitivity of surface-enhanced Raman Spectroscopy (SERS) technique. The imprinted cavity serves as the only channel for Raman reporter to approach the SERS substrate, providing highly complementary non-covalent binding sites that selectively capture the target protein based on ionic, hydrogen bonding or hydrophobic interactions. Specific recognition of the NDKB protein will perfectly fill the imprinted cavity, which makes it difficult for the Raman reporter to get close to the SERS substrate, and the Raman signal decreases significantly, while the proteins of other structural sizes can not match the imprinted cavity. Through the change of the Raman signal, the proposed biosensor can realize the ultra-sensitive detection of NDKB, and the limit of detection (LOD) is 0.82 pg/mL. Compared with the traditional immunoassay technology, this combined approach with the advantages of low cost, fast response, high sensitivity and selectivity, provides clinical application potential for the early diagnosis of CRC.
研究领域:
癌症生物学
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分析化学
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生物医学工程
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医学诊断
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2026
·
IF 7.8
Xiaoman Wei,Xuezheng Hou,Hui Zhang,Hanjie Wang,Lei Wang,Huanyu Cheng,Li Yang
Leucine (Leu) is a promising biomarker for metabolic health and muscle growth, offering significant potential for assessing physical well-being. Sweat sensors for Leu detection eliminate the reliance on invasive blood analysis and the sophisticated, large-scale instrumentation. Current sweat sensors, however, are complex to fabricate, exhibit low sensitivity toward nonelectroactive Leu, require intense exercise or thermal/chemical stimulation to generate sweat, and lack reusability. This work reports a flexible, highly sensitive, and reusable sweat sensor based on molecularly imprinted polymers, Prussian blue nanoparticles, and laser-induced graphene. When integrated with a highly permeable porous Poly(vinyl alcohol) hydrogel for convenient and rapid collection of instantaneous sweat secreted from the fingertip, the sensor can continuously detect Leu with high sensitivity (7641 nA mm–2 per decade), low detection limit (10 nM), and excellent repeatability. This flexible biosensing patch offers a promising strategy for noninvasive sweat Leu analysis and wearable health monitoring, showing potential for assessing health status related to obesity, type 2 diabetes (T2DM), and muscle loss.
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DOI
PubMed
高影响因子
Journal of Food Measurement and Characterization
·
2026
·
IF 3.6
Zhang Xia,Sun Yizhan,Ben Yingying,Liu Ru,Lv Shuwen,Xu Yuandong,Li Peng
An electrochemical molecularly imprinted polymer (MIP) sensor is developed for sensitive detection of bisphenol A (BPA) based on leaf-like Cu 2 S/activated functionalized carbon nanotubes (AFCNTs) nanocomposite as the conductive substrate. The Cu 2 S/AFCNTs composite shows a BET specific surface area of 24.7 m 2 g − 1 , which is approximately eight times higher than that of the pure Cu 2 S (3.3 m 2 g − 1 ). This enlarged surface area contributes to abundant accessible active sites and promotes efficient electron transfer. The electroactive area of the Cu 2 S/AFCNTs-modified electrode is 0.40 cm 2 which is fivefold higher than the bare electrode (0.08 cm 2 ). The MIP film is electropolymerized onto the composite surface with a thickness of 2.91 nm. Mechanistic studies confirm that BPA oxidation is an adsorption-controlled irreversible process involving 2H + /2e − . Under optimized conditions, the sensor displays a broad linear range from 0.9 to 20 µM and a limit of detection of 0.19 µM with high selectivity against common interferents. The sensor also demonstrates good reproducibility (RSD 4.2%) and stability. Practical applicability is validated by determination of BPA in milk samples, with recoveries ranging from 99% to 102.1% (RSD 2.1 ~ 3.7%). This work highlights the synergistic enhancement of Cu 2 S and AFCNTs for constructing high-performance MIP-based electrochemical sensors for food safety monitoring.
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高影响因子
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2025
·
IF 2.6
Sun Yiwei,Luo Yuting,He Yuyang,Wang Haoxiang,Wang Ruoyu,Lu Mengqi,Li Ke,Wu Siqi,Wei Sucan,Song Huiqiao,Xu Dongpo
A highly efficient electrochemical molecularly imprinted sensor for the detection of chloramphenicol (CAP) was prepared using carbon nanofibers (CNFs). The conductivity and large surface area of CNFs improved the electrochemical detection performance while providing more binding sites for chloramphenicol molecularly imprinted polymers. CNF@MIP was prepared using polypyrene as the functional monomer and chloramphenicol as the template molecule. The electrochemical performance of CNF@MIP was evaluated with cyclic voltammetry (CV) and differential pulse voltammetry (DPV). Under optimized conditions, CNF@MIP exhibited a wide detection range (0.1–100 nM), low detection limit (1.15 pM), and good selectivity, stability, and reproducibility. In addition, CNF@MIP can be used to detect chloramphenicol in milk samples.
研究领域:
分析化学
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食品科学
·
生物医学工程
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Haoxiang Wang,Yuyang He,Ruoyu Wang,Mengqi Lu,Yuting Luo,Yiwei Sun,Dongpo Xu
An electrochemical sensor with a dual recognition strategy was designed using molecularly imprinted polymers (MIPs) and boric acid for the highly sensitive detection of rutin. The synthesized metal-organic framework (MOFs) is combined with phenylboronic acid, which increases the recognition area of phenylboronic acid and its recognition ability towards adjacent diol compounds. Meanwhile, the molecularly imprinted polymer (MIP) provides specific recognition for rutin. The dual recognition strategy is widely adopted in sensors, enhancing the specificity of the imprinted cavity and effectively addressing the influence of interfering substances in complex matrices. After optimizing the experimental parameters, the detection range of this sensor is from 1 nM to 200 nM, and the limit of detection is 0.43 nM, indicating that this sensor has high potential for practical applications.
研究领域:
分析化学
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生物传感
·
药物分析
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）