Bioenergy crops provide enormous renewable biomass resources convertible for biofuel production, but lignocellulose recalcitrance fundamentally determines its enzymatic saccharification at high cost and low efficiency. In this study, total 30 diverse Miscanthus lignocellulose substrates were incubated with T. reesei strain to secret lignocellulose-degradation enzymes, and their major wall polymers features (cellulose crystallinity , hemicellulose arabinose and lignin H-monomer) were meanwhile examined with distinct impacts on the enzyme activities . Using characteristic Miscanthus (Msi62) de-lignin residue as inducing substrate with the reesei strain, this study detected that the Msi62-induced enzymes were of consistently higher enhancements on enzymatic saccharification of various lignocellulose residues examined in 17 grassy and woody bioenergy crops, particularly for the hemicellulose hydrolyses , compared to other two reesei -secreted cellulases and three commercial enzymes. Notably, based on SDS-gel protein separation profiling and LC-MS/MS analysis, the Msi62-induced enzymes consist of distinct cellulases (CBHI, BG, EGII) compositions and high-activity xylanases. Therefore, this study has demonstrated an applicable approach to achieve the optimal cellulases and xylanases cocktails that enable for low-costly and high-efficient enzymatic saccharification of diverse lignocellulose sources, providing a potential strategy for large-scale biofuel production in all major bioenergy crops.
Miscanthus lutarioriparius ( ML ) is an ideal energy crop due to its high biomass production and cellulose quality. In this study, ML was pretreated with formic acid combined with alkaline hydrogen peroxide ( FA-AHP ) to enhance glucose concentration during enzymatic hydrolysis. The physicochemical properties and enzymatic hydrolysis efficiency of ML lignocellulose following different pretreatment methods were investigated. The physicochemical results showed that cellulose fractionated by FA-AHP pretreatment ( FA-AHP-C ) became more hydrophilic, with a reduction in the degree of polymerization from 1,040 (untreated ML ) to 515 ( FA-AHP-C ). Compared to untreated ML , FA-AHP-C showed a 9.49-fold increase in cellulose conversion and a 20.61-fold increase in glucose concentration during enzymatic hydrolysis. For FA-AHP-C , the maximum cellulose conversion reached 99.86% at 5% solid loading, with optimal enzyme dosing at 10 FPU/g. Tween-80 had the greatest impact on improving enzymatic hydrolysis efficiency, increasing the cellulose conversion by 7.5% at 20% solid loading. The highest glucose concentration of FA-AHP-C reached 223.04 g/L under 34% solid loading through a fed-batch enzymatic hydrolysis process. This research offers technical support for producing high glucose concentration from ML .
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