ACS Applied Nano Materials
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2024
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IF 5.3
Kun Wang,Yingru Tang,Kangli Xing,Xiaoming Mou,Xiaoyan Yang,Zhenlan Quan
Protein self-assembly has attracted extensive interest in the field of biomaterials and biomedicine due to its inherent biocompatibility and flexible designability. In this paper, we propose a potential route to generate a photoelectrochemical (PEC) sensing platform through Sn-triggered protein self-assembly. Specifically, Sn2+ ion was used to trigger the self-assembly of bovine serum albumin (BSA) to form in situ Sn/BSA film on the indium tin oxide (ITO) glass. Then, a CdS thin film as a PEC active material was electrodeposited on the Sn/BSA modified ITO electrode. The hybrid Sn/BSA film was demonstrated to stabilize the PEC response of the CdS. A dual-electrode PEC cell comprised a graphene oxide (GO)-modified photocathode and a Sn/BSA hybrid film/CdS-loaded photoanode. Pb2+-response deoxyribozymes (DNAzymes) were hybridized with the substrate strands tagged with Au nanoparticles (Au NPs) and then immobilized on the photocathode. Upon Pb2+-induced cleavage, Au NP-linked oligonucleotide fragment dislocated from the photocathode surface, resulting in a decrease in the PEC response. The present PEC biosensor showed highly sensitive detection of Pb2+ with a wide detection range from 10.0 fM to 0.1 μM and a low detection limit of 4.0 fM. This work demonstrates that the Sn/BSA hybrid film has potential application prospects in PEC sensing, thereby providing a new way of thinking for the design and development of the bioinspired biosensor.
研究领域:
生物材料
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生物医学
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分析化学
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DOI
高影响因子
RSC Advances
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2016
·Vol. 6
·期 10
·7765-7771
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IF 3.289
Ruipeng Hou,Xiaoqing Niu,Fengling Cui
A highly sensitive and selective label-free biosensor was designed for DNA determination based on target-induced opening of the hairpin DNA and forming a G-quadruplex with protoporphyrin IX (PPIX) as a signal reporter. In the absence of target-DNA (T-DNA), the probe-DNA (P-DNA) forms a hairpin structure. A G-rich sequence in the strand is partially caged in the stem-loop structure and cannot fold into a G-quadruplex. In the presence of T-DNA, the specific combination of targets with the sensing sequence (loop sequence) triggers the release of the G-rich sequence and allows it to fold properly and bind with PPIX resulting in the fluorescence of the G-quadruplex–PPIX providing the readout signal for the sensing event (detection limit of 3.5 nM). Based on the higher efficiency of Pb2+ at stabilizing G-quadruplexes than K+ and the Pb2+-stabilized G-quadruplexes not binding to PPIX, which resulted in fluorescence decrease, the proposed DNA-sensing system can be further exploited as a Pb2+-sensing method. The detection limit for Pb2+ is 2.6 nM.
研究领域:
分析化学
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生物化学
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环境科学
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产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2017
·
IF 6.065
Wang Ren,Ying Zhang,Yu Zhu Fan,Jiang Xue Dong,Nian Bing Li,Hong Qun Luo
A resonance Rayleigh scattering (RRS) aptasensor was fabricated for detection of Pb 2+ via hairpin-like label-free substrate and G-wire for signal amplification. A hairpin-like DNA substrate contains a sequence in the loop labeled with ribonucleobase A and c-myc sequence in the stem. When hybridized with 8–17 DNAzyme in the presence of Pb 2+ , the sequence in the loop was activated and cleaved. Hundreds of c-myc sequences departing from the 8–17 DNAzyme yield nanowires superstructure called G-wire in the presence of Mg 2+ . The polymer G-wire was demonstrated by the RRS spectrum, polyacrylamide gel electrophoresis, and AFM. The RRS intensity was enhanced by the product G-wires, and the RRS signal at 370 nm was linear with the logarithm of Pb 2+ concentration in the range of 2.0 nM to 5.0 μM. This method was selective for Pb 2+ even coexisting with other metal ions at high concentrations and was successfully applied to the determination of Pb 2+ in real samples. The aptasensor holds a great promise for universal RRS sensing platform for sensitive detection of various metal ions just by changing the sequence of the probe in the loop and DNAzyme.
研究领域:
分析化学
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环境科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
International Journal of Electrochemical Science
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2023
·
IF 1.5
Daiwu Chen,Fenfang Deng,Cong Ding,Yu Wang,He Li
A novel niobium doped nickel oxide nanoparticle (NbNiO) was synthesized by sol-gel method and was applied to fabricate DNA biosensor for detection of Pb 2+ in water sample for the first time. The amino-modified G-rich DNA probes were immobilized on the surface of CH-NbNiO-MWCNTs/ITO electrode by the covalent interaction between the amino of DNA and the carboxyl of MWCNTs. When the lead (II) ion (Pb 2+ ) interacted with the G-rich DNA, it will induce the DNA conformational to switch from a random-coil to a G-quadruplex resulting in an increase of alternating current impedance. On the basis of this, we developed an impedance biosensor for the detection of Pb 2+ . The results showed that the synergistic effect of the synthesized NbNiO nanoparticles and MWCNs could improve the performance of this DNA biosensor. Under optimal conditions, the fabricated DNA biosensor possess a wide linear range of 1.0×10 -10 to 1.0×10 -5 M (correlation coefficient, R=0.998) and a low detection limit of 7.51 ×10 -12 M (3σ).
研究领域:
分析化学
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环境科学
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产品关联
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
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2023
·
IF 9.8
Limin Jing,Qijun Ding,Xia Li,Jiang Lou,Zhuqing Liu,Yifei Jiang,Wenjia Han,Zheng Cheng
In this work, fluorescent adsorbents that can efficiently detect and remove Pb 2+ were developed by integrating the designed amino-modified carbon quantum dots and carboxyl-modified collagen. The adsorption properties of the fluorescent adsorbent were further optimized and analyzed using a series of response surface experiments. The maximum adsorption concentration for Pb 2+ was 183 mg.g −1 . The adsorption isotherms fit well with the Langmuir model, and the adsorption kinetics fit with the pseudo-second-order model. The emission intensity of the fluorescent adsorbent gradually decreased with the increase of the concentration of Pb 2+ , and had a good linear correlation. In addition, the mechanism of detection and removal of Pb 2+ by fluorescent adsorbents was further demonstrated. The novel three-dimensional structured fluorescent aerogel can be used as a promising adsorbent with good adsorption concentration and sensing ability for Pb 2+ , which shows great prospects in wastewater.
研究领域:
环境科学
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材料科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Hai-Bo Wang,Li-Hong Ma,Bi-Yun Fang,Fang Tan,Yuan-Cheng Cao,Yuan-Di Zhao,Xue-Bin Hu
PS2M aptamer was selected as sensing element to construct Pb 2+ strip biosensor, and for the first time, AuNP-DNA polymer was used as sensitivity enhancement probe (SEP) to improve sensitivity. Pb 2+ separated the double-strand formed by PS2M and its complementary sequence at Anti-PS2M and induced PS2M to form G-quadruplex structure, then single-strand Anti-PS2M was released. When migrated along the strip, sequence at one end of Anti-PS2M hybridized with AuNP-DNA probe G immobilized on conjugate pad, when the composite migrated to test zone (TZ), other end of Anti-PS2M hybridized with biotin-DNA probe T on TZ to form sandwich structure. Subsequent SEP hybridized with the remaining oligonucleotides of AuNP-DNA probe G on TZ thus enhanced the signal. After optimization, the false positive result could be eliminated and the best signal value could be obtained. The results showed that the limit of detection of Pb 2+ strip biosensor was 2.5 nM by adding the sensitivity enhancement probe, which was 40 times better than that strip without enhancement. The test strip showed good results in the direct detection of soil and lake samples, presenting good practical value. In addition, the cost of this strip biosensor was low, with good market prospect.
研究领域:
分析化学
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环境科学
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生物传感器
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纳米技术
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Xinying Han,Yutong Shan,Hai Zhao,Jing Guo,Yongchun Fu,Yang Zhao,Huan Wang
Driven by the urgent demand for ultrasensitive detection of trace lead (Ⅱ) ions (Pb 2+ ), we developed a novel electrochemical sensor based on oxygen vacancy-engineered NiCo 2 O 4 (NCO) nanorods anchored on coconut shell-derived porous biochar (BC). The BC/NCO composite was prepared via a synergistic NaHCO 3 -assisted one-step carbonization activation and hydrothermal metal oxide doping strategy, which simultaneously introduced abundant oxygen vacancies and optimized the heterostructure interface. The unique rod-like morphology of NCO, coupled with abundant oxygen-containing functional groups on BC, creates synergistic adsorption-catalytic sites for Pb 2+ . The BC/NCO-modified glassy carbon electrodes (GCE) demonstrate exceptional sensitivity (24.90 µA·µM −1 ), outperforming bare GCE (0.60 µA·µM −1 ), BC-modified GCE (7.92 µA·µM −1 ), and NCO-modified GCE (4.90 µA·µM −1 ) by 42.03-fold, 3.14-fold, and 5.08-fold, respectively. This performance is attributed to oxygen vacancy-mediated electron transfer acceleration, redox pair interactions (Co 2+ /Co 3+ , Ni 2+ /Ni 3+ ), and enhanced Pb 2+ adsorption via complexation and pore confinement. The sensor achieves an ultralow detection limit of 0.004 µM (S/N = 3) and maintains robust anti-interference performance against common coexisting interferences (Cd 2+ , Cu 2+ , Fe 2+ , Ni 2+ , Zn 2+ , Fe 3+ , Hg 2+ , As 3+ , Cr 6+ , catechol, hydroquinone, resorcinol), with 98.3 −106.5 % recovery in real water samples. This work not only advances the design of eco-friendly sensing platforms through defect engineering and biomass valorization but also demonstrates high practicality for on-site heavy metal monitoring.
研究领域:
材料科学
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环境科学
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分析化学
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纳米技术
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电化学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Zhichang Ma,Jing Sun,Hemeiling Cai,Junwei Di
A photoelectrochemical (PEC) sensor, utilizing the semiconductor BiVO 4 and gold nanoparticles (AuNPs) modified with glutathione (GSH), was successfully fabricated for the sensitive detection of Pb 2+ ions. The calcinated BiVO 4 -modified indium tin oxide (ITO) electrode (ITO/BiVO 4 ) generated a low cathodic photocurrent response under light irradiation . The photocurrent at ITO/BiVO 4 /AuNPs photoelectrode increased significantly due to the plasmon enhancement of AuNPs and the electron transfer. This enhancement photocurrent intensity was largely suppressed by introducing GSH to form ITO/BiVO 4 /AuNPs/GSH. It minimized the base photocurrent of the photoelectrode. Upon the addition of Pb 2+ ions in the cell, they were adsorbed on the electrode surface through the interaction with the two carboxyl groups in GSH. Subsequently, Pb 2+ ions, serving as electron acceptors , underwent reduction under light irradiation, leading to increase in photocurrent intensity again. Therefore, a signal "on-off-on" PEC sensor was developed for the detection of Pb 2+ ions. The concentration of Pb 2+ ions show a good linearity between 0.1 pM and 10 μM with a detection limit of 0.08 pM (S/N = 3). The prepared PEC sensor shows high sensitivity, a wide linear range, and good selectivity. This work provides a promising platform for the analytical detection of heavy metal ions .
研究领域:
分析化学
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环境科学
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材料科学
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电化学
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产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2021
·
IF 10.588
Xiaohong Liu,Libo Li,Fang Li,Wanlin Zhao,Lijun Luo,Xiaoya Bi,Xia Li,Tianyan You
Signal amplification provides an effective way to improve detection performance. Herein, an ultrasensitive electrochemiluminescence (ECL) aptasensor for Pb 2+ detection was developed based on a dual signal-amplification strategy of the abscission of a quencher and the generation of a G-quadruplex by one-step and simultaneous way. Nitrogen-doped carbon quantum dots linked with complementary DNA (cDNA-NCQDs) at the sensing interface was applied as the quencher of a tris(4,4′-dicarboxylic acid-2,2′-bipyridyl)ruthenium(II) (Ru(dcbpy) 3 2+ )/tripropylamine system to minimize the ECL signal due to the intermolecular hydrogen bond-induced energy-transfer process. Upon the addition of Pb 2+ , its specific binding with the aptamer triggered the abscission of cDNA-NCQDs, accompanied by the formation of G-quadruplex on the surface of the electrode, both of which amplified the intensity of the light emission. The ECL amplification efficiency induced by the above two mechanisms (78.6%) was valuably greater than that of their sum value (69.3%). This synergistic effect resulted in high detection sensitivity of the ECL aptasensor, which allowed to thereby obtain Pb 2+ measurements in the range of 1 fM - 10 nM with an ultra-low detection limit of 0.19 fM. The Pb 2+ -mediated synergistic signal-amplification ECL strategy can provide a new approach for integrating various amplification strategies.
研究领域:
分析化学
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环境科学
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材料科学
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纳米技术
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）