FITOTERAPIA
·
2025
·
IF 2.6
Jinyuan Yang,Zhaojing Chen,Yikun Ren,Chunlei Yuan,Senhua Hu,Yiyang Liu,Dahong Li,Huiming Hua
Phytochemical investigation of Thalictrum baicalense Turcz. roots, traditionally used for inflammatory conditions, led to the isolation of three novel isoflavones, thalibaicalins A-C ( 1 – 3 ), and a new lignan, (7 R , 8 S , 7′ R , 8′ S )-9 E -feruloylneoolivill ( 6 ), alongside six known compounds ( 4 – 5 , 7 – 10 ). Structural characterization was accomplished by HRESIMS, comprehensive NMR analysis, and quantum chemical calculations. Anti-inflammatory evaluation in lipopolysaccharide-stimulated RAW 264.7 macrophages identified potent NO inhibitors, with lignan 6 exhibiting the highest activity (IC 50 = 9.56 ± 2.03 μM). This study expands the phytochemical diversity of T. baicalense Turcz. and highlights its potential as a source of anti-inflammatory lead compounds, particularly the highly active lignan derivative.
研究领域:
植物化学
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药理学
·
药物化学
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天然产物研究
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炎症生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
·
2019
·Vol. 108
·期 3
·1077-1084
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IF 2.674
Zhaoliang Guo,Qiannan Zhao,Yuqian Zhang,Bingdong Li,Lijuan Li,Liwei Feng,Manman Wang,Xianguang Meng,Guifu Zuo
An oxidized, porous graphitic carbon nitride nanosheets (CNNs) was successfully obtained via a bottom-up approach. The inner filter effect (IFE) of Fe (III) tetrakis (1-methyl-4-pyridyl) porphyrin pentachlorideporphyrin pentachloride (FeTMPyP) on the CNNs results in fluorescence quenching of the CNNs due to the overlap of FeTMPyP absorbance band and CNNs emission band. It is interesting that the quenched fluorescence could be “turned on” in response to the participation of H 2 O 2 , which caused by decomposition of FeMPyP. In this study, for the first time, a porous fluorescence probe based on CNNs and FeTMPyP was designed and an excellent H 2 O 2 detection performance with a large detection range of 0.1 ~ 100 μM and a detection limit of 0.07 μM was achieved. Furthermore, the proposed method was successfully used for H 2 O 2 detection in RAW 264.7 cells.
研究领域:
分析化学
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生物医学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2018
·Vol. 8
·期 46
·26371-26376
·
IF 2.936
Qiu Li,Qun Li,Zhihui Hao,Xucai Zheng,Wei He
We report a novel water-soluble polysaccharide from Rhizoma panacis japonica, namely, PJ-1 with anti-inflammatory activity. We first obtained a polysaccharide from this herb by eluting with water. After fully characterizing PJ-1 with a series of chromatographic technologies, we evaluated its biological activity by stimulating Raw 264.7 cells to express inflammatory cytokines and found that PJ-1 effectively enhanced the production of anti-inflammatory factors such as IL-10. We further identified its anti-inflammatory effect in a mouse model of sepsis induced by a lipopolysaccharide. The treatment of PJ-1 evidently suppressed the secretion of major inflammatory cytokines and increased the survival rate of mice. Particularly, we verified the STAT3 signal pathway responsible for the anti-inflammatory activity. Thus, PJ-1, which has remarkable anti-inflammatory effect and mechanism of action, may be a promising candidate as an anti-inflammatory agent.
研究领域:
药理学
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免疫学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
Foods
·
2022
·Vol. 11
·期 21
·3538
·
IF 5.561
Kaiying Jia,Min Wei,Yao He,Yujie Wang,Hua Wei,Xueying Tao
Exopolysaccharide (EPS) from probioticEnterococcus hiraeWEHI01 was isolated and purified by anion exchange chromatography and gel chromatography, the results of which show that the EPS consists of four fractions, namely I01-1, I01-2, I01-3, and I01-4. As the main purification components, I01-2 and I01-4 were preliminarily characterized for their structure and their immunomodulatory activity was explored. The molecular weight of I01-2 was 2.28 × 104Da, which consists mainly of galactose, and a few other sugars including glucose, arabinose, mannose, xylose, fucose, and rhamnose, while the I01-4 was composed of galactose only and has a molecular weight of 2.59 × 104Da. Furthermore, the results of an evaluation of immunomodulatory activity revealed that I01-2 and I01-4 could improve the viability of macrophage cells, improve phagocytosis, boost NO generation, and encourage the release of cytokines including TNF-α and IL-6 in RAW 264.7 macrophages. These results imply that I01-2 and I01-4 could improve macrophage-mediated immune responses and might be useful in the production of functional food and medications.Keywords:Enterococcus hiraeWEHI01;exopolysaccharide;immunomodulatory properties
研究领域:
微生物学
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免疫学
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药学
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食品科学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Jing-yan Guo,Xin-ru Song,Yan-nian Wang,Hui-ming Hua,Shu-meng Ren,Ying-ni Pan,Kun Ren,Yong-fang Sun,Dong-mei Wang,Xiao-qiu Liu
Frankincense (FRA), the oily resin consisting of essential oils, boswellic acids (BAs) and polysaccharides, has been used to improve the blood circulation and relieve pain against carbuncles. According to the theory of traditional Chinese medicine, vinegar processed frankincense (VPF) can increase the effects of promoting blood circulation and relieving pain. Existing studies have carried out much on BAs and essential oils. However, the comparative analysis of polysaccharides from FRA and VPF has not been reported. In this paper, two polysaccharides were isolated and purified from FRA and the other two were from VPF, and their structures and physicochemical properties were analyzed. The immunological and anticoagulatant activities of the four polysaccharides were tested in RAW 264.7 cell and Sprague-Dawley rats, respectively. The polysaccharides purified from VPF showed better immunological and anticoagulatant activities than those in FRA. Therefore, polysaccharides may be one of the active substances for the synergistic effect of VPF.
研究领域:
药理学
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传统中药学
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生物化学
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免疫学
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血液学
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产品关联
DOI
PubMed
高影响因子
Foods
·
2024
·Vol. 13
·期 24
·4019
·
IF 4.7
Zhou Xu,Yanxia Xiong,Pei Hu,Long Chen,Jianhua Wan,Chenggang Huang,Wenjun Liu
Alpinia officinarumHance has a medicinal history of thousands of years in treating cough, diabetes, and gastrointestinal system diseases, and it is also a medicine food homology (MFH) plant in China. To evaluate the pharmacological activities of polysaccharides from the rhizomes ofA. officinarum, polysaccharides were initially obtained by hot-water extraction and the ethanol precipitation method. A homogenous polysaccharide designated as AOP-w was isolated by a DE-52 column. The proposed structure was elucidated and the immunoregulatory effects on RAW 264.7 macrophage cells were evaluated. The results showed that AOP-w had a molecular weight of 5.26 kDa, and mainly consisted of galactose and glucose (molar ratio of 0.12:0.88). Its backbone comprised α-(1→4)-Glcp, α-(1→4,6)-Glcpand β-(1→3,4)-Galpresidues, terminated by α-(1→6)-Glcpand T-Glcpresidues. AOP-w was nontoxic to RAW 264.7 cells, but demonstrated promotion in cell proliferation within a 100 μg/mL concentration. The immunostimulatory effects of AOP-w were confirmed by the elevated NO production of AOP-w-treated cells. Moreover, the RNAseq was conducted and the results showed that AOP-w may activate the TNF and NF-κB signaling pathways by binding to Toll-like receptors, thereby affecting the immune modulatory activity of RAW264.7 cells. These results suggest a high potential of AOP-w fromA. officinarumfor immunotherapeutic applications.
研究领域:
药理学
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分子生物学
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免疫学
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产品关联
DOI
PubMed
高影响因子
NATURAL PRODUCT RESEARCH
·
2020
·
IF 2.158
Sen-Ju Ma,Hai-Bo Li,Jun-Ran Shao,Qian-Qian Pang,Ting Li,Xin-Sheng Yao,Yang Yu
A new phenylpropanoid allopyranoside (1) and a new indolinone alkaloid (2) were isolated from the rhizomes of Actaea dahurica (syn. Cimicifuga dahurica). The structures of those two compounds were deduced as cimicifugaside F (1) and 3E,11E-(3-methyl-2-butenylidene acid)-2-indolinone-1-O-β-d-glucopyranoside (2) by detailed analysis of their MS, 1D and 2D NMR data and comparison with literatures. Additionally, the isolates were evaluated for their inhibitory effects on the production of NO by LPS-stimulated RAW 264.7 macrophages.
研究领域:
植物化学
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药理学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2016
·Vol. 51
·期 3
·664-671
·
IF 1.504
Zhen Wu,Daodong Pan,Xiaoqun Zeng,Yangying Sun,Jinxuan Cao
Summary Peptidoglycan (PG) is available from a wide variety of lactic acid bacteria (LAB) and is the main structure of cell wall components. Phosphorylated modification would bring new properties such as the potential antioxidant activities and antiviral capability to an organic molecule. In the present work, small molecular fragments of PG (derived from Lactobacillus acidophilus ) hydrolysed by mutanolysin were phosphorylated under optimal conditions. P-PG had a monomer molecular structure of GlcNAC[PO 3 ]–MurNAC–Ala–Glu–Lys–Ala, with a molecular mass of 884 Da and a phosphorus content of 8.9%. P-PG displayed some immunoregulatory activity in lipopolysaccharides (LPS) stimulated RAW 264.7 macrophages. Compared with the LPS-stimulated group, the addition of P-PG inhibited the secretion of GM-CSF, TNF-α and IL-1 in a dose-dependent manner. The effect of 50 μg mL −1 of P-PG was more significant than 50 μg mL −1 of PG. Lower fluorescence of lysosomes was observed in P-PG-treated RAW 264.7 cells may also reveal some immune defence function in the LPS-induced macrophages.
研究领域:
微生物学
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免疫学
·
分子生物学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2016
·
IF 3.138
Hong Niu,Dan Song,Haibo Mu,Wuxia Zhang,Feifei Sun,Jinyou Duan
Mushroom Inonotus obliquus ( I. obliquus ), a folk medicine, has been widely used to treat several human malicious tumors since 16th century. In this study, three homogenous biomolecules (designated IOA1, IOA2 and IOA3) were prepared from the alkali extract of I. obliquus . Their molecular weights were measured to be 6.1 × 10 4 , 2.9 × 10 4 and 3.5 × 10 4 g/mol respectively and all of them were characterized as lignin–carbohydrate complexes mainly comprised lignin as well as −25% carbohydrates. Antioxidant assays indicated that all of them exhibited pronounced reductive power and strong scavenging activities on DPPH and hydroxyl radicals in vitro . Immunological tests showed that they could also significantly stimulate nitric oxide production and phagocytic activity in RAW 264.7 macrophages. These results implied that the lignin–carbohydrate complexes extracted from I. obliquus might be used as novel natural antioxidants or immunostimulants in functional foods or pharmaceutical candidates.
研究领域:
药理学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Shasha Cheng,Yuzhen Hou,Xue Wen,Dawei Xu,Xianzhu Luo,Hongshuai Cao
Real-time monitoring of nitric oxide from endogenously cells released is crucial for revealing their physiological activities or pathological processes. However, designing electrochemical sensors for real-time monitoring at the cellular level is challenging due to the simultaneous requirements of sensitivity, transient recording capability, and biocompatibility. Combining the universally self-supported grown highly active materials with stretchable conductive flexible substrates holds promise for achieving sensitive detection of cells and real-time monitoring of mechanically induced biomolecular release. However, existing manufacturing strategies for highly sensitive stretchable flexible electrochemical sensors still face significant challenges due to low material activity, high processing requirements, and the complexity of further designing membrane electrodes. Here, we designed a method of in-situ self-supported growth to grow Co-doped Ni(OH) 2 structures on rigid Ti foil. Leveraging the synergistic effect of Co and Ni, sensitive detection of NO was achieved, enabling real-time monitoring of trace amounts of NO released by Raw 264.7 cells. Based on this material, using catalytic ink method, it was transferred to low-resistance flexible ITO film (LIF), enabling real-time monitoring of mechanically induced NO release from Human Umbilical Vein Endothelial Cells (HUVECs) cells.
研究领域:
生物医学工程
·
材料科学
·
分析化学
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产品关联
DOI
高影响因子
Journal of Analytical Methods in Chemistry
·
2023
·
IF 2.6
Qianchun Zhang,Haijiang Du,Siqi Xie,Fengling Tian,Xixi Long,Shan Liu,Yun Wu
Hypochlorite (ClO−) has received extensive attention owing to its significant roles in the immune defense and pathogenesis of numerous diseases. However, excessive or misplaced production of ClO− may pose certain diseases. Thus, to determine its biological functions in depth, ClO− should be tested in biosystems. In this study, a facile, one-pot synthesis of nitrogen-fluorine-doped carbon quantum dots (N, F-CDs) was developed using ammonium citrate tribasic, L-alanine, and ammonium fluoride as raw materials under hydrothermal conditions. The prepared N, F-CDs demonstrate not only strong blue fluorescence emission with a high fluorescence quantum yield (26.3%) but also a small particle size of approximately 2.9 nm, as well as excellent water solubility and biocompatibility. Meanwhile, the as-prepared N, F-CDs exhibit good performance in the highly selective and sensitive detection of ClO−. Thus, a wide concentration response range of 0–600 μM with a low limit of detection (0.75 μM) was favorably obtained for the N, F-CDs. Based on the excellent fluorescence stability, excellent water solubility, and low cell toxicity, the practicality and viability of the fluorescent composites were also successfully verified via detecting ClO− in water samples and living RAW 264.7 cells. The proposed probe is expected to provide a new approach for detecting ClO− in other organelles.
研究领域:
分析化学
·
生物医学
·
环境科学
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产品关联
DOI
PubMed
高影响因子
CHEMISTRY & BIODIVERSITY
·
2025
·
IF 2.5
Zebin Huang,Wanying Zhang,Qi Shu,Xiao-Chun Guo,Xi Zheng,YuJing Lu
Inflammation is an important predisposing factor for many chronic diseases. The dietary flavonoid silibinin has excellent anti-inflammatory properties in cells, but its low bioavailability in the blood compromises its therapeutic potential. This study aims to investigate the potential of dibenzoylmethane (DBM) to synergistically enhance the anti-inflammatory benefits of silibinin (SB). The synergistic effects of DBM and SB in combination were evaluated in LPS-induced RAW264.7 cells and TPA-induced mice. In addition, a network pharmacology approach and molecular docking were used to explore the key targets and signaling pathways of DBM and SB in combination. The results showed that DBM and SB synergistically inhibited the production of NO, ROS, IL-1β, and TNF-α in a 1:1 concentration ratio. These two compounds may exert their synergistic effects by modulating the NF-κB and HIF-1 signaling pathways, among others. Molecular docking revealed that both compounds exhibited high binding affinities to iNOS and COX-2. Compared with single compound use, the two compounds in combination significantly reduced ear edema and inflammatory cell infiltration, and inhibited the protein expression of iNOS and COX-2 in TPA-induced mice. This research provides a rationale for the combination of DBM and SB as an effective anti-inflammatory agent.
研究领域:
药理学
·
免疫学
·
分子生物学
·
炎症研究
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产品关联
DOI
PubMed
高影响因子
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