ANALYST
·
2024
·Vol. 149
·期 4
·1318-1326
·
IF 3.6
Kaida Kuang,Ya Li,Yang Chen,Yu Ji,Nengqin Jia
Conventional single-signal or emerging sandwich-type double-signal electrochemiluminescence (ECL) immunosensors/aptasensors have offered accurate detection of small molecules, yet suffer from complicated setup, long processing time, and non-reusability. Here, we demonstrate a simplified molecularly imprinted ECL sensor based on Mn2SnO4 nanocubes. As an n-type semiconductor, Mn2SnO4 has numerous active sites that can capture electrons to accelerate chemical reactions, resulting in enhanced ECL activity and stability. For the first time, we verify a robust cathodic ECL emission of Mn2SnO4 luminophores in the presence of K2S2O8 coreactants. The proposed ECL sensor applies to the sensitive detection of ribavirin (RBV), endowing a wide linear range (1–2000 ng mL−1), low detection limit (0.85 ng mL−1, S/N = 3), high stability, specificity, and reproducibility, and the detection capability in real milk and chicken samples. This work highlights single semiconductor luminophore-driven molecularly imprinted ECL sensors, meeting the original aspiration of uncomplicated but high-performance sensing in food safety inspection.
研究领域:
分析化学
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生物医学工程
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食品安全
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DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Junlei Zheng,Lin Nie,Yukui Tong,Miaomiao Tian
Ribavirin (RBV) in food and environmental samples presents significant risks to human health, necessitating the development of methods capable of its simultaneous detection and adsorption. This study describes the preparation of a multi-functional molecularly imprinted material (Nylon@Eu-MOF@MIPs), achieved through in-situ growth of Eu-MOF with fluorescence properties on a nylon membrane, designed for the concurrent adsorption and detection of RBV. The material demonstrates high adsorption capacity for RBV (41.36 mg g −1 ), a pronounced imprinting effect (imprinting factor of 3.06), and substantial sensitivity (detection limit of 0.01 μM). Furthermore, following ten adsorption–desorption cycles, Nylon@Eu-MOF@MIPs retain effective adsorption capacity, with a minimal loss of 5.8 %. Additionally, a filtration-enhanced adsorption and detection mode is proposed, achieving a 1.63-fold increase in adsorption capacity compared to the traditional soaking method. The integrated platform developed in this study facilitates efficient adsorption and visual detection of RBV. This research not only advances membrane separation materials but also establishes foundational conditions for creating integrated systems for simultaneous adsorption and visual detection.
研究领域:
材料科学
·
环境科学
·
分析化学
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DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2026
·Vol. 14
·期 4
·123314
·
IF 7.5
Junhao Wang,Xinrui Qin,Jiajia Ge,Yongtao Cheng,Kang Li,Jun Liu,Kang Shan,Chuanyue Peng,Wenjing Yan,Juan Wang,Min Zuo,Ying Liu
Abstrct Microalgae are capable of removing various organic pollutants through their inherent processes of growth, reproduction and biological metabolism. In present study, a microalgae-based fuel cell was constructed using Chlorella sp. SI-50, significantly improving the removal efficiency of the antiviral ribavirin (RBV) from wastewater. Compared with conventional microalgal systems, which typically achieve only 70% removal of RBV within 18 days, the present system achieved complete elimination (100%) of 5 mg/L RBV by day 6. Furthermore, the microalgal antioxidant enzyme system exhibited outstanding antioxidant activity, which was attributed to oxidative stress from microcurrent and electron transfer. Transcriptomic analysis screened phosphotransferase and nucleotide transferase as key enzymes involved in RBV biodegradation. These enzymes participate in purine/pyrimidine metabolism and xenobiotic detoxification, potentially catalyzing RBV oxidation and conjugation. This work highlights that microalgal electrochemical systems efficiently enhance the remediation of antiviral contaminants and reveal core molecular mechanisms underlying RBV metabolism, providing a promising eco-friendly strategy for the treatment of pharmaceutical wastewater.
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DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2025
·
IF 4.1
Ziqi Wang,Yahui Ji,Nana You,Xiaoping Hu,Fangxin Du,Gen Liu
Testing ribavirin (RBV) is essential to promote its appropriate clinical use, mitigate resistance development, reduce adverse effects, and safeguard public health. Electrochemiluminescence (ECL) outperforms conventional methods in RBV detection, offering superior sensitivity and reduced background noise. In this work, ECL micro-reactors were prepared by incorporating Ru(bpy) 3 2+ into a mesoporous silica matrix with two-dimensional hexagonal structure (SBA-15). In this configuration, tripropylamine (TPA) was employed as a co-reactant, which interacted with Ru(bpy) 3 2+ to enhance the ECL signal. The quenching effect of ribavirin (RBV) on the Ru(bpy) 3 2+ -TPA system was confirmed through its significant suppression of ECL emission. A glassy carbon electrode (GCE) modified with SBA-15 loaded with Ru(bpy) 3 2+ (SBA-15@Ru) was fabricated as a novel ECL sensor. This sensor enabled the detection of RBV over a wide concentration range 1.0 × 10 −9 mol·L −1 to 1.0 × 10 −5 mol·L −1 , achieving a detection limit (LOD) of 3.33 × 10 −10 mol·L −1 . Additionally, the sensor exhibited outstanding stability and selectivity and was successfully applied for accurate quantification of RBV in pharmaceutical samples.
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高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2021
·
IF 8.742
Muning Chen,Jian Lu,Jia Gao,Chao Yu,Wendong Xing,Jiangdong Dai,Minjia Meng,Yongsheng Yan,Yilin Wu
Powerful drivers exist for the exploitation of high natural life materials to selectively separate valuable substances or pollutants. Molecularly imprinted membranes (MIMs) possessing high selectivity and stability are highly desirable. Carefully designed Ribavirin (RBV)-imprinted membranes (RBVMIMs) with remarkable self-cleaning ability as well as anti-fouling and antibacterial surfaces enable selective separation of RBV from industrial effluent produced by the RBV process. (i) Self-cleaning ability was achieved by the photo-Fenton catalytic activity of RBVMIMs based on Fe 2 O 3 nanoparticles . (ii) Anti-fouling and antibacterial performance were provided by a multifunction hybrid coating of polydopamine (pDA)-quaternary ammonium compounds (QAC). (iii) Satisfactory selectivity was stemmed from the uniform presence of RBV-imprinted sites which synthesized by “thiol-ene click reaction” on the membrane surface. Nfioteworthily, the employment of two functional monomers (methacrylic acid and acrylamide) synergistically promoted selective recognition of RBV-imprinted sites. The results indicated that satisfactory rebinding selectivity (αRBV/ACV = 9.87, αRBV/AZT = 9.21, αRBV/LAM = 6.63 and αRBV/G = 13.59) and permselectivity ( β AZT/RBV = 11.361, β LAM/RBV = 12.518, β ACV/RBV = 30.918 and β G/RBV = 18.732) with negligible permselectivity fluctuation (<10%) of RBVMIMs suggested the potential of RBVMIMs in practical applications, which was further confirmed by superior performance in semi-practical experiments. Importantly, the development and modification strategies developed in this work not only guided selective separation of valuable constituents from complex environments but also provided the reference for the development of membrane with high natural life.
研究领域:
环境科学
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材料科学
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生物医学工程
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DOI
高影响因子
JOURNAL OF FOOD SCIENCE
·
2021
·Vol. 86
·期 7
·2851-2860
·
IF 3.167
Jianyu Zhu,Qiang Li,Xuezhi Yu,Xiya Zhang,Hongfang Li,Kai Wen,Yuebin Ke,Suxia Zhang,Zhanhui Wang
Ribavirin (RBV) is an effective antiviral drug, whose use is prohibited in animal husbandry worldwide. In this work, a novel immunizing hapten of RBV, named Hapten 4, was designed by comparing the conformational and electronic properties of RBV and haptens based on computational chemistry. Hapten 4 was synthesized and conjugated with carrier proteins to produce monoclonal antibody (mAb). The obtained mAb 4C3 for RBV exhibited an IC 50 value of 6.24 ng/ml in an indirect competitive enzyme-linked immunosorbent assay (icELISA) and displayed no cross-reaction with five other antiviral drugs, including amantadine. The applicability of the developed icELISA was verified in chicken, with a calculated limit of detection of 4.23 µg/kg. The recoveries in spiked chicken were 79.2%–107.3% with a coefficient of variation less than 15.9%. The results indicated that the produced antibody from the new hapten was reliable and would be useful for RBV screening in chicken. Practical Application RBV is a broad-spectrum antiviral drug, which is commonly used illegally in poultry farms. A high-affinity mAb 4C3 against RBV was produced and used to develop icELISA with acceptable sensitivity and accuracy. The constructed icELISA has excellent performance for detecting RBV residues in chicken.
研究领域:
免疫学
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药物分析
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食品安全
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DOI
PubMed
高影响因子
BMC Chemistry
·
2023
·Vol. 17
·期 1
·1-10
·
IF 4.6
Xu Daokun,Huang Haolun,Hu Wenyan,Liu Xinmei,Yang Jun
A liquid chromatography–tandem mass spectrometry (LC–MS/MS) method was developed for the analysis of ribavirin in chicken. Samples was extracted with 0.1% formic acid and purified by Hypercarb cartridge prior to LC–MS/MS analysis. The eluates were evaporated to dryness, reconstituted in 1 mL 5mM ammonium acetate containing 5% acetonitrile (v/v) and 0.1% (v/v) formic acid. Chromatographic separation was performed on a Hypercarb analytical column under a gradient elution program with acetonitrile and 0.1% (v/v) formic acid in 5 mM ammonium acetate at a flow rate of 0.6 ml/min. The intraday and interday accuracy ranged from − 7.83 − 1.39%, and − 6.38 − 2.25%, with precisions between 1.34 − 3.88%% and 1.10 − 4.67%. The limits of detection (LODs) and limits of quantitation (LOQs) of ribavirin was 0.1 ng/mL and 0.5 ng/mL, respectively. The method was validated for linearity, accuracy, precision, matrix effect and stability. Application of the method confirmed 3 ribavirin positive samples out of 50 commercial chicken samples, with concentrations of ribavirin ranging from 0.9 μg/kg to 5.8 μg/kg a, respectively. Additionally, both AB Sciex 5500 and Agilent 6945B were proven to be suitable in ribavirin separation and quantification. The described method is suitable for the determination of ribavirin in chicken in analytical practice to monitor illegal addition of this kind of anti–viral drug.
研究领域:
分析化学
·
兽药残留检测
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食品科学
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DOI
PubMed
高影响因子
DESALINATION
·
2025
·
IF 9.8
Yilin Wu,Yanke Xu,Faguang Ma,Jingjing Zhen,Yue Li,Fanlei Kong,Ming Yan
In this study, modified hollow e-ZIF-8 nanoparticles with a hollow structure and hydroxyl-rich surface were ingeniously synthesized through a one-step etching method, thereby increasing the contact area and addressing the issue of stacking imprinting sites, fundamentally enhancing selectivity. Among these, the hollow structure of the e-ZIF-8 nanoparticles not only increased the interlayer spacing of the graphene oxide (GO) nanosheets, providing additional pathways for water molecules, but also, through π-π interactions, cross-linked the phenol-modified nanoporous metal-organic frameworks (MOFs) with the GO nanosheets, significantly enhancing the structural stability and anti-fouling capability of the composite membrane. The effects of different e-ZIF-8 addition amounts on membrane flux and hydrophilicity were investigated, constructing a GO-based imprinting structure on the stacked e-ZIF-8/GO nanosheet surfaces, offering a novel strategy to improve the selectivity and stability of the membrane. The maximum adsorption capacity of e-MG-IMs reached 38.35 mg g −1 , demonstrating good selective separation factors ( α RBV/ACV = 3.24, α RBV/AZT = 2.41 and α RBV/LAM = 4.21), as well as excellent permeation selectivity ( β ACV/RBV = 4.75, β AZT/RBV = 4.42 and β LAM/RBV = 5.92). In this study, the optimized regulation of GO nanosheets extended the molecular transport channels, facilitating the construction of more molecular recognition sites, thus positively impacting the separation performance of the imprinting membrane.
研究领域:
材料科学
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药物分离工程
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环境科学
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DOI
高影响因子
COLLOID AND POLYMER SCIENCE
·
2025
·
IF 2.3
Mao Xiaoxi,Zhou Haijing,Wang Xiaotian,Jiao Enguang,Gao Meng,Wang Kunhua,Guan Meili,Ning Liangmin,Fu Min,Chen Wei
Ribavirin (RBV) is a broad-spectrum antiviral agent with proven clinical efficacy. Improper RBV disposal however may cause substantial ecosystem contamination and human health hazards, attributable to its confirmed developmental toxicity. Consequently, operationally simple RBV extraction strategies urgently needed for environmental waters. In this work, leveraging the superior chemical durability, extensive active surface area, and eco-friendly nature of biomass-activated carbon (BAC), a novel molecularly imprinted polymer (PC@PEI/PBA/MIPs) was engineered through dopamine-mediated surface polymerization using rapeseed pollen carbon (PC) as the substrate, specifically designed for selective RBV separation. Furthermore, branched polyethyleneimine (PEI) was covalently grafted onto the porous carbon surface to add the density of boronic acid groups, resulting in a high density of recognition sites in a mild environment. The adsorption experiments show that PC@PEI/PBA/MIPs has a significant binding efficiency (158.03 mg /g) for RBV, and can quickly reach the adsorption equilibrium time within 60 min, with good selectivity and regeneration performance. Finally, PC@PEI/PBA/MIPs were used to capture RBV from real water samples, achieving > 80% recovery efficiency. The prepared PC@PEI/PBA/MIPs plays a decisive role in the separation and enrichment of RBV in complex systems.
研究领域:
环境科学
·
材料化学
·
分析化学
·
高分子科学
·
水污染控制
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DOI
高影响因子
LANGMUIR
·
2025
·
IF 3.9
Chen Chen,Fei Wu,Ting Cheng,Fuqiang Liu,Xiao Zhang,Baoxuan Hou,Li Song,Yuan Tian,Xin Zhao,Safi Ullah,Rui Li
The unregulated zinc dendrite formation, together with undesirable parasitic side reactions, has posed major obstacles to the commercialization and stability of aqueous zinc-ion batteries (AZIBs). Herein, as an additive in the electrolyte of AZIBs with dual functions, ribavirin, a small molecule antiviral drug, was innovatively employed to tackle these issues. Simulation and experimental findings consistently revealed that the incorporation of ribavirin effectively suppressed the formation of zinc dendrites and markedly enhanced the stability of the reaction system. Ribavirin disrupted the stable coordination structure of the ZnSO4 electrolyte. Mediated by hydrophilic interactions from its hydroxyl groups, ribavirin decreased the number of water molecules within the solvation shell, which in turn enhanced the dynamic freedom of zinc ions and adjacent water molecules, thereby markedly promoting zinc-ion diffusion during battery operation. Additionally, ribavirin molecules preferentially adsorbed onto the zinc sheet surface, forming a protective layer that not only effectively mitigated the corrosive effects of the ZnSO4 electrolyte but also facilitated electron transfer and inhibited the hydrogen evolution reaction (HER). Moreover, the introduction of ribavirin into the ZnSO4 electrolyte facilitated three-dimensional (3D) ion diffusion, leading to a smoother zinc deposition process and a remarkable suppression of zinc dendrite growth during battery cycling. Benefiting from this dual function of coordination regulation and surface adsorption, the Zn//Cu asymmetric cells with ribavirin maintained consistent performance over 1000 charge/discharge cycles, still exhibiting a stable Coulombic efficiency of 98.6% at the 950th cycle, while the Zn//Zn symmetric cells demonstrated exceptionally stable cycling for over 2800 h at 1 mA·cm–2 and over 1050 h at 2 mA·cm–2, concurrently delivering enhanced Coulombic efficiency and superior rate performance. Furthermore, in V2O5-based full cells, ribavirin enhanced zinc-ion transport, improved specific capacity, and enabled stable cycling for over 2000 cycles.
研究领域:
电化学
·
储能
·
材料科学
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电池技术
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DOI
PubMed
高影响因子
Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure & Risk Assessment
·
2018
·
IF 2.129
Zhaopeng Wang,Xuezhi Yu,Licai Ma,Hebing Liu,Shuangyang Ding,Zhanhui Wang,Xiya Zhang,Jianzhong Shen,Kai Wen
In this work, high affinity polyclonal antibodies for ribavirin (RBV) from new haptens were prepared and were used to analyse RBV residues in chicken muscle, eggs and duck muscle. The new haptens were synthesised with different spacers, and the best antibody was obtained with an IC50 value as low as 0.61 ng/mL in indirect competitive enzyme-linked immunosorbent assay (ELISA). The cross-reactivities with another five antiviral drugs including amantadine, rimantadine, moroxydine, zanamivir and oseltamivir were less than 0.1%, which indicated the good specificity of the antibody. An ELISA was developed based on the antibody and applied to detect RBV in multi-food matrices. The sample preparation prior to detection only needed simple dilution after trichloroacetic acid extraction. The limits of detection were 1.07, 1.18 and 1.03 μg/kg in chicken muscle, eggs and duck muscle, respectively. Recoveries ranged from 89.0% to 112.7% with coefficients of variation below 13.0%. Ten blind samples of chicken muscle were analysed simultaneously by ELISA and liquid chromatography-tandem mass spectrometry, and a good correlation between the methods was observed. The results indicated that the high affinity antibody could be applied for the simple and fast detection of RBV in multi-food matrices.
研究领域:
免疫学
·
食品科学
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分析化学
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DOI
PubMed
高影响因子
Analytical Methods
·
2022
·Vol. 14
·期 45
·4669-4679
·
IF 3.532
Lvcheng Liu,Yuqi Ling,Junshan Han,Tingting Hao,Xing Li
Solvated mercuric ions (Hg2+), a toxic and harmful water pollutant, can easily accumulate in organisms and cause serious damage to the kidney, liver, and central nervous system. To realize rapid and efficient detection of mercury (II) ions in water sources, a kind of new colorimetric sensor of gold nanoparticles (AuNPs) functionalized with ribavirin (Rib-AuNPs) was proposed and characterized by TEM, DLS, XRD, and UV-vis in this work. The color of the Rib-AuNP solution rapidly changed from wine-red to gray-blue with the addition of Hg2+ based on the aggregation mechanism. The limits of detection (LODs) are 0.20 μM by the naked eye and 3.64 nM by UV-vis spectroscopy with a fine linear relationship in the range of 0–0.25 μM (R2 = 0.9834) and 0.25–0.80 μM (R2 = 0.9893) of Hg2+, indicating that the detection system of Rib-AuNPs could be applied to analyze Hg2+ with excellent selectivity and anti-interference in real water samples.
研究领域:
环境科学
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分析化学
·
纳米技术
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）