JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Aimin Song,Zhiheng He,Luqing Pan,Xin Yu,Yaobing Li
Polycyclic Aromatic Hydrocarbons (PAHs), as persistent organic pollutants widespread in marine environments, significantly impact ocean ecosystems. However, their effects on the taste quality of aquatic animals and the underlying mechanisms remain unknown. This study investigated the changes in content and metabolism of taste substances in Ruditapes philippinarum under PAHs stress. The results showed that PAHs reduced the umami and sweet tastes of R. philippinarum but enhanced its bitterness. In addition, the EUC value in soft tissue of R. philippinarum was significantly decreased by PAHs. The expression levels of key genes showed that PAHs mainly interfere with the mTOR signaling pathway, AMPK signaling pathway, and the synthesis and decomposition processes of taste substances, altering the content of taste substances in R. philippinarum and affecting their taste quality. This study will provide scientific warning and support for the quality assurance of aquatic products and the monitoring of marine PAHs pollution.
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高影响因子
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
·
2019
·Vol. 96
·期 12
·1367-1377
·
IF 1.72
Zhong-Yuan Liu,Da-Yong Zhou,Kanyasiri Rakariyatham,Hong-Kai Xie,De-Yang Li,Bei-Wei Zhu,Fereidoon Shahidi
The current study shows the compositional changes and oxidation development of clam ( Ruditapes philippinarum , R. philippinarum ) lipids and frying oils when subjected to different processing conditions. Parameters measured include acid value, peroxide value (POV), thiobarbituric acid-reactive substances (TBARS), total oxidation (TOTOX), lipid classes, fatty acid composition, phosphatidylcholine (PC), and phosphatidylethanolamine (PE) contents together with major glycerophospholipid (GP) molecular species. Deep-fat frying increased triacylglycerol (TAG) content and decreased the contents of PC, PE, and GP molecular species in clam in a time-dependent manner. Meanwhile, minor amounts of free fatty acids, diacylglycerols, monoacylglycerols, and polar lipids were detected in frying oils, indicating lipid migration between the clam and frying oils. The time-dependent increase of POV, TBARS, and TOTOX in fried clams and frying oils with concurrent reduction of docosahexenoic acid and eicosapentaenoic acid indicates extensive oxidative degradation of clam lipids. Moreover, the moisture-rich clam aggravated the deterioration of frying oils. Consequently, deep-fat frying significantly altered the lipid profile and decreased the nutritional value of clams.
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Nanoscale
·
2022
·Vol. 14
·期 21
·7807-7816
·
IF 8.307
Hongyu Du,Yunqing Wang,Panpan Zhang,Rongchao Mei,Yunxia Ji,Xizhen Zhao,Zhiyang Zhang,Jiping Ma,Lingxin Chen
Nanoplastics (NPs) as emerging marine pollutants can be taken up by seafood organisms. It is crucial to quantitatively assess NP's distribution behavior in organisms to elucidate concentration dependent biological effects. Such a knowledge gap has remained due to the lack of reliable NP models and analytical methods. Herein, surface enhanced Raman scattering (SERS)-labeled NP models were developed and their bioavailability, distribution and accumulation in Ruditapes philippinarum, a typical marine bivalve, were quantitatively studied. Taking advantage of the sensitive and characteristic SERS signals of the NP models, distribution could be quickly and accurately obtained by the Raman imaging technique. Moreover, quantitative analysis of NPs could be performed by the detection of gold element contents via inductively coupled plasma mass spectroscopy (ICP-MS) detection. ICP-MS results revealed that after 3 days exposure of monodispersed NPs (100 nm, 0.2 mg L−1), the digestive gland accumulated 86.7% of whole-body NPs followed by gill (5.2%), mantle (5.1%), foot (1.3%), exhalant siphon (1.1%), and adductor (0.6%). Upon 11 days depuration, 98.7% of NPs in the digestive gland were excreted, whereas the clearance ratios in other organs were much lower. NP aggregates (around 1.5 μm) demonstrated similar distribution and clearance trends to the monodispersed ones. However, the accumulation amount in each organ was 15.2% to 77.6% lower. Surface adherence and passive ingestion routes resulted in NP accumulation, which contributed to the comparable NP abundance in these organs. Additionally, boiling treatment (mimicking a cooking process) did not decrease the NP amount in these organs. This work provided a dual-mode and quantitative analysis protocol for NPs for the first time, and suggested the risk of NP uptake by humans via bivalve seafood diets.
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高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Zheng Zhou,Yu-Ying Zhang,Jing-Xuan Gao,Li-Xin Ma,Xu-Hui Huang,Jie Zheng,Xiu-Ping Dong,Lei Qin
This study investigated the changes in free amino acids (FAAs), 5′-nucleotides, and lipid compositions of Manila clams ( Ruditapes philippinarum ) collected from December 2017 to November 2018. High-performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry was used to quantify twenty-one FAAs and six 5ʹ-nucleotides. Lipid profiles were explored by ultra-performance liquid chromatography coupled with a Q-Exactive Orbitrap mass spectrometer with an untargeted lipidomic approach. Results showed significant monthly changes of FAAs, 5′-nucleotides, and lipids profiles. Alanine , glutamic acid , glycine, and aspartic acid , which contributed to the sweet and umami flavor, were the most abundant FAAs in Manila clams. Totally 90 glycerides, 76 glycerophospholipids , 21 lyso-glycerophospholipids, and 52 fatty acids were identified in Manila clams. Among these lipids, most of them possessed a high-level of unsaturation degree. The unsaturation degree of lipids in February, March, and April was the highest, showing the best nutritional value of Manila clams.
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CHEMOSPHERE
·
2024
·
IF 8.1
Lu Wang,Jingjing Miao,Min Ding,Wei Zhang,Luqing Pan
Nonylphenol (NP) contamination in the coastal environment of China poses ecological risks to aquatic organisms. However, the endocrine disruptive impacts of NP on bivalves, particularly on ovarian development, remain poorly understood. In this study, Manila clams Ruditapes philippinarum at the developing stage of gonad were exposed to 1.0 μg/L NP for 21 days. Utilizing RNA interference (RNAi) to suppress ER gene expression, we observed a delay in ovarian development as evidenced by histological observations under both NP and NPRi (NP with ER-RNAi) treatment, with Vtg elevation exclusive to the NP group. Comprehensive analyses encompassing transcriptomics, real-time quantitative PCR, and steroid hormone measurement revealed significant alterations in aldosterone synthesis, estrogen signaling, and thyroid hormone synthesis. These pathways showed similar perturbations in both NP and NPRi groups compared to controls. Notably, the NPRi group exhibited distinct enrichment in PPAR and insulin signaling pathways, may implicating these in ER function suppression. Steroid hormone biosynthesis was notably reduced in both treatments, pointing to a profound impact on hormone synthesis. The contrast between in vivo and in vitro findings suggests that NP's detrimental effects on ovarian development may primarily involve neuroendocrine regulation of steroidogenesis. This investigation highlights the complex dynamics of NP-induced endocrine disruption in bivalves, emphasizing the pivotal role of ER and associated pathways.
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PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2018
·
IF 3.412
Hongkai Xie,Dayong Zhou,Xiaopei Hu,Zhongyuan Liu,Liang Song,Beiwei Zhu
To predict the shelf life through an Arrhenius model and evaluate the changes in lipid profiles, two types of dried clams were stored at 50 and 65 °C and collected periodically for analysis. The predicted shelf life values of the two dried clam samples were 530 ± 14 and 487 ± 24 h (24 °C), and the relative errors between the actual and predicted values were 5.7 and 6.8%, respectively. During accelerated storage, the peroxide value, p-anisidine value, thiobarbituric acid-reactive substances value, total oxidation value, acid value, and free fatty acid content all increased, while the levels of triacylglycerol, phosphatidylcholine, phosphatidylethanolamine, major glycerophospholipid molecular species, and polyunsaturated fatty acid (PUFA) decreased. Moreover, content of phospholipid containing PUFA decreased significantly than that of triacylglycerol containing PUFA. Results indicated that the Arrhenius model was suitable for the shelf life prediction of dried clams and accelerated storage caused loss in quality of dried clams in terms of lipids.
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高影响因子
AQUACULTURE INTERNATIONAL
·
2026
·Vol. 34
·期 7
·284
·
IF 3.5
Hu Qi,Liao Kai,Zhang Yang,Liu Yang,He Xiaoxue,Xie Fengjun,Tuo Rui,Qin Qin,Xu Jilin
Bivalve planktonic larvae are extremely vulnerable to oxidative stress under intensive hatchery conditions, resulting in growth retardation and elevated mortality. This study evaluated the effects of dietary supplementation with microencapsulated vitamin C on growth, antioxidant capacity, and innate immunity in planktonic Manila clam ( Ruditapes philippinarum ) larvae. An 11-day feeding trial was conducted in cylindrical tanks using a microalgal-based diet and supplemented with 0, 0.086, 0.114, or 0.142 mg of microencapsulated vitamin C powder per gram of larval wet weight. Relative to the non-supplemented control (0 mg g −1 group), larvae fed 0.114 mg g −1 microencapsulated vitamin C showed a 33% increase in weight per tank and a 20% increase in survival rate. Following H 2 O 2 exposure, mortality at both 8 h and 12 h was significantly reduced in all vitamin C-supplemented groups relative to the control. In addition, the 0.114 mg g −1 group showed significantly higher superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities and increased reduced glutathione (GSH) content, lower malondialdehyde (MDA) content, upregulated Nrf2 pathway genes ( nrf2 and keap1 ), and downregulated gclc and gstp1 . In the same group, lysozyme (LZM), phenoloxidase (PO), and alkaline phosphatase (AKP) activities were significantly increased, while NF-κB pathway genes ( tlr4 , myd88 , cox2 , ikkα , and nfkb1 ) were significantly downregulated. Regarding proinflammatory and hypoxia-related factors, tnf-α was significantly downregulated and hif-1α was markedly downregulated in the 0.114 mg g −1 group, whereas il-17 was significantly downregulated in the 0.142 mg g −1 group. Overall, dietary supplementation with 0.114 mg g −1 microencapsulated vitamin C significantly enhanced weight per tank, survival, oxidative stress resistance, and innate immunity in R. philippinarum larvae.
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