SENSORS
·
2025
·Vol. 25
·期 5
·1630
·
IF 3.5
Qiang Xu,Huiting Yin,Mei Cui,Renliang Huang,Rongxin Su
A fiber-reinforced SPR sensor based on silver-nucleated gold-shell bimetallic nanoparticles and graphene oxide was developed and applied to human IgG detection. The refractive index (RI) sensitivity of the Ag@Au/GO fiber SPR sensor is as high as 4715.9 nm/RIU in the RI range of 1.333–1.365. Staphylococcus aureus protein A (SPA) can specifically recognize and bind to the fragment crystallizable (Fc) of the antibody; it facilitates the highly targeted immobilization of the antibody. SPA and rabbit anti-human IgG were immobilized on the surface of the Ag@Au/GO fiber SPR sensor for the detection of different concentrations of human IgG with a sensitivity of 0.53 nm/μg/mL and detection limits of 0.037 μg/mL. This biosensor based on the mixed structure of GO and Ag@Au combined the common advantages of the two materials. Therefore, our study provides a simple platform for biological analysis and has a good application prospect.
研究领域:
生物传感技术
·
材料科学
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2025
·
IF 4.7
Shishu Sun,Tianyi Sun,Dashuai Zhang,Jinrui Liu,Liying Ren,Chuilong Chen,Wei Yao,Zaifeng Shi
In this paper, the synthesis, characterization, and photocatalytic performance of CuNi/TiO 2-x composites in the degradation of antibiotic Norfloxacin (NOR) under visible light were investigated. Compared to conventional TiO 2 , the composite catalyst demonstrated superior photocatalytic activity by introducing CuNi nanoparticles and oxygen vacancy in TiO 2 . The oxygen vacancy defect in titanium dioxide crystal reduced the band gap energy. In addition, the incorporation of Cu nanoparticles leveraging Surface Plasmon Resonance (SPR) and Ni nanoparticles as electron traps further markedly enhanced the electron-hole pair separation efficiency and solar energy utilization, catalyzing NOR degradation efficiently. Electron Spin Resonance (ESR) confirmed the generation of reactive oxygen species, which was pivotal to the NOR degradation. This study underscores the potential of bimetallic composites for environmental remediation, particularly in treating antibiotic-laden wastewater.
研究领域:
环境科学
·
材料科学
·
化学工程
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
OPTICS AND LASERS IN ENGINEERING
·
2020
·
IF 4.273
Hang Song,Qi Wang,Wan-Ming Zhao
In this work, we proposed a novel surface plasmon resonance (SPR) sensitivity-enhancing method by adding MoS 2 nanosheets between gold film and fiber (fiber-MoS 2 -gold film). And we apply it to the detection of human IgG protein. The sensor was fabricated by attaching MoS 2 nanosheets to the U-shaped fiber surface and then covering a layer of gold film. The refractive index sensitivity of 6184.4 nm/RIU was achieved by the fiber-MoS 2 -gold film sensor which is 59 percent higher than the gold film SPR sensor and 25 percent than the fiber-gold film-MoS 2 SPR sensor.The presence of MoS 2 increases the absorption of light energy and excites a stronger SPR phenomenon. At the same time, since the monolayer MoS 2 is a direct bandgap semiconductor, the absorbed light energy promotes the transfer of electrons, generates strong coupling on the gold film surface, enhances the surface electric field, and improves the sensitivity of the sensor. Biofunctionalized sensor detect human IgG with the limit of detection as low as 19.7 ng/mL. This work proposed a novel method for enhancing the sensitivity of SPR sensor, it owns a higher sensitivity and a lower LOD which has broad application prospects in measuring low concentration biological analytes.
研究领域:
光学
·
生物传感
·
纳米技术
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
OPTICS EXPRESS
·
2023
·Vol. 31
·期 13
·21212-21224
·
IF 3.8
Zhuo Ren,Chunlan Liu,Yong Wei,Chunbiao Liu,Chen Shi,Xingkai Wang,Yixiong Tang,Rui Wang,Zhihai Liu
The Fiber SPR chip laboratory has become a popular choice in biochemical detection. To meet the needs of different kinds of analytes for the detection range and number of channels of the chip, we proposed a multi-mode SPR chip laboratory based on microstructure fiber in this paper. The chip laboratory was integrated with microfluidic devices made from PDMS and detection units made of bias three-core fiber and dumbbell fiber. By injecting light into different cores of a bias three-core fiber, different detection areas of dumbbell fiber can be selected, enabling the chip laboratory to enter high refractive index detection, multi-channel detection and other working modes. In the high refractive index detection mode, the chip can detect liquid samples with a refractive index range of 1.571-1.595. In multi-channel detection mode, the chip can achieve dual parameter detection of glucose and GHK-Cu, with sensitivities of 4.16 nm/(mg/mL) and 9.729 nm/(mg/mL), respectively. Additionally, the chip can switch to temperature compensation mode. The proposed multi working mode SPR chip laboratory, based on micro structured fiber, offers a new approach for the development of portable testing equipment that can detect multiple analytes and meet multiple requirements.
研究领域:
生物化学检测
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光学传感
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微流控技术
·
生物医学工程
✓ 已验证
DOI
高影响因子
TALANTA
·
2022
·
IF 6.556
Like Li,Ya-nan Zhang,Wanlu Zheng,Xuegang Li,Yong Zhao
An optical fiber SPR biosensor based on multimode fiber (MMF)- hollow core fiber (HCF)-MMF is proposed and experimentally confirmed for in situ DNA hybridization analysis. In order to improve the sensitivity of DNA hybridization detection, a sandwich model based on gold nanoparticles (AuNPs) amplification is proposed. In this model, the probe DNA (pDNA) is first modified on the optical fiber sensing area by covalent bonding, and then the biotinylated target DNA (tDNA) is modified on the AuNPs by the high affinity between biotin and streptavidin. Finally, the tDNA on the surface of the AuNPs specifically hybridizes with the pDNA on the optical fiber to form a sandwich model. The near field coupling enhancement between AuNPs and gold film and the high mass ratio of AuNPs give high sensitivity to DNA hybridization detection. Experimental results show that the sandwich-type fiber SPR sensor has a log-linear response in the DNA concentration range of 1 pM–10 nM, and the sensitivity and detection limit are 4.04 nm/log(μM) and 1pM, respectively. This is 1.44 times more sensitive than that without sandwich assay. In addition, the DNA sensor has good specificity and stability, which foreshadows its great application potential in the fields of disease diagnosis, microbial detection and environmental science.
研究领域:
生物传感
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纳米技术
·
光纤技术
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生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2020
·Vol. 45
·期 6
·3102-3111
·
IF 3.288
Tehseen Nawaz,Muhammad Ahmad,Jieying Yu,Shiqi Wang,Tianxin Wei
A novel tetracycline (TC) imprinted polymer was prepared in visible light via synergy of dual functional group monomers methacrylic acid (MAA) and itaconic acid (IA) for the selective detection of TC in urine and milk samples. Surface plasmon resonance (SPR) and the reversible addition fragmentation chain transfer (RAFT) mechanism were employed in combination with 4-cyano-4-[(dodecylsulfanylthiocarbonyl)sulfanyl]pentanoic acid (CDTPA) in the presence of ethylene glycol dimethacrylate (EGDMA) as the cross-linker. In addition, the ratio of template, IA, MAA and EGDMA was optimized (0.8 : 0.3 : 5 : 9) by real time monitoring via SPR. The surface of the TC imprinted sensor chip was characterized by contact angle measurements, Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). The (IA–MAA@SPR)MIP sensor chip displayed a high adsorption capacity and selectivity in comparison to other analogues and non-imprinted polymer (NIP) based sensor chips. Through 7 adsorption–desorption cycles, the (IA–MAA@SPR)MIP sensor chip exhibited a good recoverability. These features suggested that the IA–MAA based sensor chip was an ideal candidate for the detection of TC. Consequently, within the concentration range of 1 × 10−13–1 × 10−7 mol L−1, the reflectivity changes versus the negative logarithm of concentrations presented excellent linearity; R2 = 0.99. Based on a linear equation, the IA–MAA@SPR MIP sensor chip showed lower values for the limit of detection (LOD) and limit of quantification (LOQ) i.e. 1.38 × 10−14 mol L−1 and 4.5 × 10−14 mol L−1, respectively. Furthermore, the influence of the matrix on the sensor chip was successfully analyzed in real samples with satisfactory outcomes. Hence, IA–MAA based MIPs in combination with the SPR sensor chip demonstrated potential application prospects for rapid and effective sensing even in the complicated matrices.
研究领域:
分析化学
·
材料科学
·
传感器
·
环境科学
✓ 已验证
DOI
高影响因子
INFRARED PHYSICS & TECHNOLOGY
·
2025
·
IF 3.4
Lixia Li,Yurui Huang,Jiabin Zhao,Feiyou Liu,Ning Feng,Yufang Liu
Mercury ions (Hg 2+ ) are highly toxic heavy metal pollutants that pose significant risks to the environment and human health. In this paper, a fiber optic surface plasmon resonance (SPR) sensor is proposed for ultra-sensitive detection of Hg 2+ . The probe features Ag/ Ge 2 Sb 2 Te 5 (GST)/Ag composite film and metal–organic framework (MOF)-sensitized layer functionalized with probe DNA (pDNA). In the presence of Hg 2+ , pDNA interacts with target DNA (tDNA) carrying gold nanoparticles (AuNPs) in the solution through the T-Hg 2+ -T structure, immobilizing AuNPs on the sensing surface and inducing a spectral red shift. By combining GST’s high dielectric constant and MOF’s large surface area with strong pDNA affinity, the sensor achieves a maximum refractive index (RI) sensitivity of 11471 nm/RIU, detecting Hg 2+ as low as 10 pM. Furthermore, the deep learning model based on the residual neural network (ResNet) is employed to classify and recognize the SPR spectra corresponding to six distinct concentrations of Hg 2+ , achieving a training accuracy of 99.88 % and a test accuracy of 97.5 %. Therefore, ultra-sensitive fiber optic SPR sensor with deep learning delivers high-performance sensing while enabling rapid, precise, and sensitive quantification of Hg 2+ detection.
研究领域:
传感器
·
环境监测
·
光学传感
·
纳米技术
·
重金属检测
·
机器学习
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Chao Zhao,Jiayi Zhu,Xingye Ma,Jiaqi Sun,Ning Wang,Xi Chen,Xiaohan Chen,Yihao Wang,Chuanquan Wang,Xinyue Jing
Rapid and accurate pathogen detection is critical for optimizing clinical decision-making within precision medicine frameworks, specifically for identifying Staphylococcus aureus (S. aureus) in wound infections. Most of conventional detection methods suffer from procedural complexity, protracted analysis times, and insufficient sensitivity, thereby constraining their clinical deployment. In this work, an optical fiber surface plasmon resonance (SPR) sensor enhanced by Ti 3 C 2 T x MXene was developed for detecting S. aureus , biofunctionalized with goat anti-human immunoglobulin G (IgG). The optimal thickness of Ti 3 C 2 T x MXene layer, determined to be approximately 94 nm based on maximizing figure of merit (FOM) of 17.88 RIU −1 , yielded high sensitivity and low full width at half maximum (FWHM). By monitoring SPR wavelength shifts in the transmission spectra, the sensor was employed to detect S. aureus with a low limit of detection (LOD) of 1.14 CFU/mL and a high sensitivity of 3.063 nm/log (CFU/mL). It exhibited excellent linearity across the concentration range of 10 2 –10 8 CFU/mL, alongside high stability and selectivity. Validation using S. aureus -infected wound tissue from diabetic murine models exhibited a precision exceeding 97 %. This technology represents a promising avenue for developing next-generation, high-performance biosensing platforms with significant translational potential in precision medicine diagnostics.
研究领域:
生物传感器
·
光学传感
·
纳米材料
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生物医学诊断
·
传染病检测
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 9
·1-9
·
IF 6.232
Yin Zhenzhen,Cheng Xiaoli,Wang Gan,Chen Jia,Jin Yan,Tu Qiuyun,Xiang Juan
A highly sensitive and specific surface plasmon resonance (SPR) method using one anti-alpha-synuclein antibody (anti-αS) and titanium phosphate nanoparticles (Ti 4+ @TiP) was developed for quantitative evaluation of phosphorylated αS level which was defined by the ratio of p-αS to total alpha-synuclein (t-αS) (p-αS/t-αS). The close affinities of anti-αS to αS (0.975 pM −1 ) and p-αS (0.938 pM −1 ) were obtained. Based on this fact , both αS forms were simultaneously captured and the t-αS was quantified using the anti-αS immobilized Au chip. With the selective recognition of Ti 4+ @TiP nanoparticles, the p-αS was quantified. The dynamic ranges of our method were 1.0~20.0 pg mL −1 for the detection of t-αS and 0.1~10.0 pg mL −1 for that of p-αS. The analysis of αS- and p-αS-spiked artificial cerebrospinal fluid samples revealed the high accuracy of the method. Furthermore, the concentrations of αS and p-αS in clinical CSF samples collected from three healthy donors were determined and displayed a high correlation with the results from a commercial ELISA kit, confirming the viability and of the proposed method. The method is convenient, economical, and practical for the evaluation of phosphorylated αS level with high sensitivity and selectivity. It is of great significance for the early diagnosis of PD and the evaluation of PD progression. Graphical abstract
研究领域:
生物传感器
·
纳米技术
·
神经退行性疾病
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2020
·
IF 5.339
Haixia Yu,Yang Chong,Penghao Zhang,Jiaming Ma,Dachao Li
Fiber-based techniques make it possible to implant a miniaturized and flexible surface plasmon resonance (SPR) sensor into the human body for glucose detection. However, the miniaturization of fiber SPR sensors results in low sensitivity compared with traditional prism-type SPR sensors due to limited sensing area. In this paper, we proposed a D-shaped fiber SPR sensor with a composite nanostructure of molybdenum disulfide (MoS 2 )-graphene to improve the sensor sensitivity. Compared with the traditional cylindrical fiber, the planar sensing area on the side-polished fiber makes it easier to modify two-dimensional materials. Chemical vapor deposition (CVD) graphene and CVD MoS 2 were modified on the sensor surface to obtain the MoS 2 -graphene composite nanostructure. π-π stacking interactions were used to modify pyrene-1-boronic acid (PBA) on the graphene. The excellent photoelectric properties of the MoS 2 -graphene composite nanostructure and the ability of PBA to specifically bind glucose molecules improved the glucose detection performance of the SPR sensor. The results show that specific detection of glucose was realized and that the highest sensitivity was achieved with three-layer MoS 2 and monolayer graphene.
研究领域:
光学传感
·
纳米技术
·
生物传感器
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Jie Zhou,Youjun Zeng,Xueliang Wang,Changlin Wu,Zhiwen Cai,Bruce Zhi Gao,Dayong Gu,Yonghong Shao
In this study, a wavelength-interrogated surface plasmon resonance imaging-based immunosensor was developed for ABO blood typing. To construct the immunosensor, the detection line array was formed by the site-directed immobilization of blood group antibodies on SPR chip modified with antibody-binding proteins. Red blood cell (RBC) samples were flowed into the multichannel flow cell that was orthogonal to the detection line arrays. By serially analyzing the interaction between RBCs, antibody A, and antibody B through the observed changes in the resonance dip, blood typing of four RBC samples was achieved in a single run. Detailed investigations focused on optimizing the conditions that affected the efficiency and sensitivity of immunoassay. Under the optimized conditions, the RBC samples were appropriately grouped in less than 1 h, including chip modification time. The lowest detection limit for RBC-A and RBC-B was 2×10 6 cells/ml. The immunosensor developed through this study provided a simple, rapid, high-throughput, and sensitive immunology strategy for classification of ABO blood group.
研究领域:
生物医学工程
·
分析化学
·
免疫学
✓ 已验证
产品关联
DOI
高影响因子
Plasmonics
·
2025
·
IF 4.3
Zhang Yirui,Wang Zhiyang,Zhang Ailing,Chang Pengxiang,Li Zhen,Shi Yanmei
In this paper, a fiber-optic biosensor with the structure of both Au film and Au nanoparticles (AuNps) co-modified on D-shaped photonic crystal fiber (PCF) by adhesion with polydopamine (PDA) is proposed and experimentally fabricated, where the electric field near the sensing surface is increased and thus the sensitivity is improved by coupling the surface plasmon resonance (SPR) in Au film with the localized surface plasmon resonance (LSPR) in Au nanoparticles (AuNps), D-shaped PCF as well as high refractive index of PDA. In addition, due to excellent adhesion and biocompatibility properties of PDA, an extra layer of PDA is modified on the surface of AuNps to anchor a layer of goat anti-rabbit IgG on it, which is a bioassay for rabbit IgG. The experimental results show that the sensor obtains a high sensitivity of 4301.49 nm/RIU in the refractive index (RI) range of 1.335 ~ 1.365, which is 2.4 times the RI sensitivity of the PCF-Au sensor. The bio-sensitivity of the sensor for rabbit IgG reaches 1.017 nm/(µg/ml), and the limit of detection (LOD) is 0.019 µg/ml. This sensitized fiber-optic biosensor based on the SPR-LSPR coupling effect has a potential application in clinical disease diagnosis and immunoassay.
研究领域:
光学生物传感
·
纳米技术
·
生物光子学
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分析化学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）