NEW JOURNAL OF CHEMISTRY
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2022
·Vol. 46
·期 18
·8912-8918
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IF 3.925
Qianqian Hu,Guoning Chen,Lu Wang,Xia Cui,Chun Chang,Qiang Fu
Sarcosine is an important biomarker in the diagnosis of prostate cancer. It is essential to explore a method that detects sarcosine in biological samples quickly and accurately for the early screening and diagnosis of prostate cancer. Considering the high catalytic efficiency and good selectivity of enzymes, there is potential for establishing enzymatic methods for the determination of sarcosine. However, the poor stability, high economic consumption, and challenging recyclability of free enzymes limit their use. Here, sarcosine oxidase was immobilized to improve its stability. The properties of the immobilized enzyme, including the morphology and stability, were characterized. Then, the enzymatic reaction between the immobilized enzyme and the substrate sarcosine was employed to establish a fluorescence method with a linear range from 0.1563 to 10.00 μg mL−1, a LOD of 0.04 μg mL−1, and a recovery of 84.7–104.0%. It has been proved that sarcosine can be detected using the established method with short time consumption, high specificity, and low cost, and which is easy to operate and is affordable for many laboratories and community-level medical institutions. These results demonstrate that this method shows potential for practical applications in the diagnosis of prostate cancer.
研究领域:
生物化学
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医学诊断
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材料科学
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DOI
高影响因子
TALANTA
·
2017
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IF 4.162
Zhonghua Xue,Hui Wang,Honghong Rao,Nan He,Xiaofen Wang,Xiuhui Liu,Xiaoquan Lu
Indicator displacement assay plays a fundamental role in the development of chemosensors . We explored here an ingenious yet effective strategy for amperometric assay of electrochemically inactive sarcosine based on an indicator displacement principle, in which 1,2-naphthoquinone-4-sulphonic acid sodium salt (NQS) was proposed as the receptor and the electroactive Ru(NH 3 ) 6 3+ cations used as an indicator. Due to the stronger binding affinity of the NQS toward sarcosine than toward Ru(NH 3 ) 6 3+ , the developed amperometric indicator displacement assay (A-IDA) exhibits high selectivity and excellent sensitivity toward sarcosine determination as well as with a lower detection limit (30.00 nM, S/N =3).
研究领域:
分析化学
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生物医学工程
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电化学传感器
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DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2019
·
IF 9.518
Qia Wang,Yuting Zhao,Qingui Yang,Dan Du,Haipeng Yang,Yuehe Lin
Amperometric enzyme biosensors are some of the simplest and cheapest types of medical devices used in the rapid detection of biomarkers that have been developed in the past fifty years. When the concentrations of biomarkers are at micromoles per liter, such as for sarcosine , which was recently discovered as a biomarker for prostate cancer, the response signal of the interferences is huge, and the biosensor is hard to satisfy the requirements of practical applications. In this manuscript, we describe a strategy for synthesizing a surface electronegative organic-inorganic hybrid mesoporous material , which could reduce the interference signal much better than Nafion and Chitosan. We verify that the surface potential of the carrier nanomaterial plays an important role in excluding anionic interferences. We also prepare a sensitive (16.35 μA mM −1 ), low LOD (0.13 μM) and wide linear range (1–70 μM) amperometric sarcosine biosensor with excellent anti-interference properties. This mesoporous material provides a bio-composite platform for the development of simple amperometric biosensors for detecting micromoles per liter of analytes in serum or urine.
研究领域:
生物医学工程
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分析化学
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癌症诊断
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DOI
PubMed
高影响因子
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
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2020
·Vol. 86
·期 17
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IF 4.016
Ting Yang,Ya-Hui Shao,Li-Zhong Guo,Xiang-Lin Meng,Hao Yu,Wei-Dong Lu
Chromohalobacter salexigens DSM 3043 can grow on N,N-dimethylglycine (DMG) as the sole C, N, and energy source and utilize sarcosine as the sole N source under aerobic conditions. However, little is known about the genes and enzymes involved in the conversion of DMG to sarcosine in this strain. In the present study, gene disruption and complementation assays indicated that the csal_0990, csal_0991, csal_0992, and csal_0993 genes are responsible for DMG degradation to sarcosine. The csal_0990 gene heterologously expressed in Escherichia coli was proven to encode an unusual DMG dehydrogenase (DMGDH). The enzyme, existing as a monomer of 79 kDa with a noncovalently bound flavin adenine dinucleotide, utilized both DMG and sarcosine as substrates and exhibited dual coenzyme specificity, preferring NAD+ to NADP+. The optimum pH and temperature of enzyme activity were determined to be 7.0 and 60°C, respectively. Kinetic parameters of the enzyme toward its substrates were determined accordingly. Under high-salinity conditions, the presence of DMG inhibited growth of the wild type and induced the production and accumulation of trehalose and glucosylglycerate intracellularly. Moreover, exogenous addition of DMG significantly improved the growth rates of the four DMG– mutants (Δcsal_0990, Δcsal_0991, Δcsal_0992, and Δcsal_0993) incubated at 37°C in S-M63 synthetic medium with sarcosine as the sole N source. 13C nuclear magnetic resonance (13C-NMR) experiments revealed that not only ectoine, glutamate, and N-acetyl-2,4-diaminobutyrate but also glycine betaine (GB), DMG, sarcosine, trehalose, and glucosylglycerate are accumulated intracellularly in the four mutants.IMPORTANCE Although N,N-dimethylglycine (DMG) dehydrogenase (DMGDH) activity was detected in cell extracts of microorganisms, the genes encoding microbial DMGDHs have not been determined until now. In addition, to our knowledge, the physiological role of DMG in moderate halophiles has never been investigated. In this study, we identified the genes involved in DMG degradation to sarcosine, characterized an unusual DMGDH, and investigated the role of DMG in Chromohalobacter salexigens DSM 3043 and its mutants. Our results suggested that the conversion of DMG to sarcosine is accompanied by intramolecular delivery of electrons in DMGDH and intermolecular electron transfer between DMGDH and other electron acceptors. Moreover, an unidentified methyltransferase catalyzing the production of glycine betaine (GB) from DMG but sharing no homology with the reported sarcosine DMG methyltransferases was predicted to be present in the cells. The results of this study expand our understanding of the physiological role of DMG and its catabolism to sarcosine in C. salexigens.
研究领域:
微生物学
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生物化学
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DOI
PubMed
高影响因子
ANALYST
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2025
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IF 3.3
Yiwen Sun,Ying Xie,Zixuan Yuan,Yujiao Mao,Huiru Liu,Yiting Zhu,Jinyu Dong,Xiaojun Chen
As a significant biomarker for the early development of prostate cancer (PCa), the sensitive detection of sarcosine (SA) in urine can serve as an effective non-invasive early warning method. Herein, we developed novel a hollow double shell NiMn PBA (NiMn-PBA-DSNB) with outstanding peroxidase-like activity through a facile cation exchange reaction according to the distinct solubility product constants (Ksp) of different Prussian blue analogues (PBAs). Moreover, we have innovatively proposed a “enzyme - nanozyme” cascade strategy to realize ultrasensitive SA sensing. This system utilized the enzymatic reaction between SA and sarcosine oxidase (SOX) to generate H2O2 in situ. In the presence of the generated H2O2, the NiMn-PBA-DSNB nanozyme can efficiently catalyze the oxidation of 3, 3’, 5, 5’-tetramethylbenzidine (TMB) to produce the blue oxTMB. Consequently, the concentration of SA directly correlated with the absorbance value of oxTMB, providing a quantitative detection. Based on this dual-enzyme cascade mechanism, a highly sensitive colorimetric assay was developed for the precise analysis of SA. The assay achieved a wide linear range from 8 to 500 μM, with a low detection limit of 1.75 μM, fully fulfilling the requirements for SA sensing in the urine of PCa patients. The method was also successfully applied to the analysis of real human urine samples, achieving recoveries ranging from 102.15% to 104.35% with relative standard deviations ≤ 5.03%, which demonstrates its strong potential for clinical application.
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DOI
PubMed
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
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2022
·Vol. 169
·期 7
·077512
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IF 4.386
Wenhuang Xiao,Xiaolin Zhao,Luyin Lin,Jian Luo,Haipeng Yang
Sarcosine has been discovered as a better potential biomarker for Prostate cancer (PCa). Though many high-performance amperometric sarcosine biosensors have been reported, it is still difficult to accurately detect sarcosine because of the extremely low concentration and the presence of many electroactive interferents in human serum or urine. Since most of the reported sarcosine biosensors use platinum group metals (PGM) as catalysts, it is meaningful to explore other catalysts with higher catalytic activity. Metal-nitrogen-doped carbon (M–N–C) materials are considered as alternatives to precious metals. In this study, iron doped zeolitic-imidazolate-framework-8 (ZIF-8) composites were synthesized and calcined to obtain an Iron-nitrogen-doped Carbon (Fe–N–C) material, which has excellent catalytic activity. The sensitivity of the Fe–N–C modified sarcosine biosensor is 16.5 μA mM−1, (234.2 μA mM−1 cm−2), which is the highest one in recent reported works. It has a low limit of detection (LOD, 0.7 μM, S/N = 3), and an appropriate linear detection range of 2–37 μM. This work provides a new approach to prepare high performance sarcosine biosensors by improving the catalytic activity of the modifier of the sensor. It has great potential to be used as portable devices for the rapid detection of PCa.
研究领域:
医学
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生物化学
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材料科学
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DOI
高影响因子
ELECTROCHIMICA ACTA
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2023
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IF 6.6
Xiaofang Zhang,Wenhuang Xiao,Shenghui Xie,Gaochao Fan,Xinlin Shi,He Meng,Haipeng Yang
Hydrogen peroxide (H 2 O 2 ) is an important signaling molecule that regulates many biological processes. It is of great value to realize rapid, accurate, highly sensitive and low-cost H 2 O 2 detection in the immediate detection of trace disease markers. Low-density nanoelectrode arrays have a high signal-to-noise ratio and are advantageous for trace detection. In this paper, low-density Pt nanoelectrode arrays were successfully prepared by a simple method of ion sputtering combined with electrodeposition based on a track-etched polycarbonate membrane template. The arrays had a detection limit as low as 0.03 μM for H 2 O 2 at a constant potential, a sensitivity of 125.9 μA mM −1 , and a linear range spanning nearly four orders of magnitude (0.1–655 μM). The developed sarcosine sensors based on these arrays had a very low detection limit (0.08 μM) and wide linear range (0.5–64.5 μM) for the rapid detection of trace amounts of sarcosine in serum. With a wide linear range and low detection limits, this hydrogen peroxide sensing platform has great potential for use in the rapid electrochemical detection of trace substances.
研究领域:
分析化学
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生物传感器
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纳米技术
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医学诊断
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产品关联
DOI
高影响因子
Analytical Chemistry Letters
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2024
·
IF 1
Sara Salatin,Ali Asghar Hamidi,Ali Abolhassani,Samin Hamidi
Sarcosine has emerged as a potential prostate cancer marker, yet current detection methods have limitations, necessitating the development of non-invasive approaches for early and accurate diagnosis. To develop a novel, sensitive method combining dispersive micro-solid phase extraction with high-performance liquid chromatography for sarcosine determination in urine samples. A meso-sized graphene oxide@chitosan adsorbent was synthesized via a template-free, freeze-drying method. The adsorption mechanism was investigated using Langmuir and Freundlich isotherm models, and extraction parameters were optimized for maximum efficiency. Under optimized conditions, the method showed linearity over 0.05-5 µg/mL with precision better than 10%. The adsorption data demonstrated better correlation with the Langmuir model, indicating monolayer adsorption. Regeneration studies using acetonitrile and water washing cycles demonstrated the adsorbent’s reusability for up to 5 extraction cycles, with approximately 70% retention of initial extraction capacity. The method exhibited high sensitivity and good precision using a simple setup. This approach offers a practical, non-invasive method for sarcosine determination in urine, potentially contributing to improved prostate cancer screening and monitoring. The reusability of the adsorbent enhances its economic viability and aligns with green chemistry principles, though some capacity loss occurs after multiple cycles, likely due to memory effects or structural changes in the porous framework.
研究领域:
生物医学工程
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分析化学
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癌症诊断
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DOI
高影响因子
Journal of Saudi Chemical Society
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2020
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IF 3.517
Pingping Tang,Yaobin Wang,Feiyu He
Accurate determination of Sarcosine (SAR) in urine with high sensitivity and selectivity is important, because it was recently recommended as a prospective biomarker for prostate cancer (PCa) and significant for the early identification of PCa. In this study, an electrochemical sensor based on Fe 3 O 4 incorporated metal–organic frameworks (MOFs) @molecularly imprinted polymer (MIP) was constructed for SAR detection. Magnetic Fe 3 O 4 nanoparticles embedded zeolitic imidazolate framework-8 (ZIF-8) was used as the support of MIP. MIP provides specific recognition sites for template molecules SAR and MOFs increase the rate of mass transfer and adsorption capacity due to the porous structure. The synthesized super-magnetic Fe 3 O 4 @ZIF-8@MIP was self-assembled onto an Au electrode in magnetic field and used as the sensing unit of electrochemical sensor. Cyclic voltammetry was used to monitor the electrochemical behavior, and the binding of SAR resulted in a reduction in the measured current. The results revealed a wide linear range from 1 to 100 pM towards trace SAR determination, with extremely low limit of detection down to 0.4 pM. In conclusion, the Fe 3 O 4 @ZIF-8@MIP based sensor provides a selective, sensitive, and convenient method for SAR diagnosis and other cancer marker detection.
研究领域:
生物医学工程
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分析化学
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癌症诊断
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
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2026
·
IF 5.2
Xu-yang Guo,Renagul Abdurahman,Xing-hong Pan,Rui Zhang,Ling-hua Zuo,Rui-hong Xu,Tao Li,Xue-Bo Yin,Ming Guan
First synthesis of Mn-CeO 2 @ZnGa₂O₄:Cr³⁺, Eu³⁺ composite. • Trimodal sarcosine detection using phosphorescence, peroxidase mimicry, and photothermal TMB. • Smartphone-based mobile tool enables on-site sarcosine detection. • Paper-based chip sensor simplifies sarcosine detection, making it facile and convenient. Prostate cancer (PCa) is the second most common cancer in men, while sarcosine levels have profound implications for the state of prostate cancer. Herein, we have designed a smart paper-chip sensor based on Mn-CeO₂ nanozyme@persistent luminescent nanoparticles (PLNPs) for trimodality fluorescence, colorimetric, and photothermal detection of sarcosine. The PLNPs were prepared as ZnGa₂O₄: 0.5% Cr 3+ , 1.5% Eu 3+ and then integrating Mn-CeO₂ nanozyme. The composite was integrated onto a polypropylene (PP) substrate as the paper chip. In pa1rticular, PLNPs exhibited the afterglow emission for eliminating the interference from the autofluorescence of biological samples; In the presence of both sarcosine and sarcosine oxidase, red phosphorescence is restored, for phosphorescence detection. The nanozyme of Mn-doped CeO₂ exhibit enhanced peroxidase activity, rapidly converting colorless 3,3′,5,5′-tetramethylbenzidine (TMB) into a blue form as oxidized TMB, which also possesses photothermal conversion capabilities, enabling both colorimetric and photothermal detection. Using a smartphone, this sensor enables on-site detection of sarcosine in serum with a detection range of 0–10 μM with extremely low limits of detection (LOD) as 1.88 μM for fluorescence; 0.08 μM for colorimetry; and 1.88 μM for photothermal mode, with all RSD below 5%. Overall, we have successfully designed for the detection of sarcosine in serum. The sensor's low cost, high sensitivity, and portability have been validated, thereby improving treatment success rates and survival rates for patients with early-stage prostate cancer. Scheme 1. Schematic illustration of a smartphone-based Mn-CeO₂@persistent luminescence nanoparticles (PLNPs) paper-based sensor for triple-mode detection of sarcosine. ZnGa₂O₄: Cr 3+ , Eu 3+ (ZCEGO) was fabricated as PLNPs featuring red emission at 700 nm. The PLNPs were surface-functionalized with Mn-CeO₂ nanozymes as peroxidase mimics, and the resultant nanocomposite was further incorporated onto a paper-based substrate. In the presence of sarcosine and sarcosine oxidase (SOX), H₂O₂ is generated. In Zone 1 (yellow section), the PLNPs are exposed, enabling phosphorescence detection. Meanwhile, in the presence of TMB, TMB in Zone 2 (white section) is oxidized to oxTMB, enabling colorimetric detection. The oxTMB can be used for photothermal detection upon irradiation with an 808 nm laser. Quantitative analysis of sarcosine is achieved using a smartphone. Download: Download high-res image (102KB) Download: Download full-size image
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高影响因子
CHEMICAL ENGINEERING JOURNAL
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2021
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IF 13.273
Zhiqiang Ma,Lijun Yang,Yutong Wang,Mengfan Wang,Wei Qi,Zhimin He
Peptide assembly provides a new approach for the construction of enzyme-like catalysts. However, low stability is still a big challenge for the peptide-based enzyme mimics because the non-covalently driven structures are often destroyed when exposed to environmental changes. Inspired by the catalytic centre of natural horseradish peroxidase (HRP) and the biological function of dityrosine in structural proteins, in this study, we presented the construction of a stable peptide-based HRP mimic (CCA-YH) through introducing covalent bonds . CCA-YH was obtained through the co-assembly of YYHYY and hemin, followed by a ruthenium-mediated photo-crosslinking reaction. Catalytic activity assay demonstrated that CCA-YH displayed enhanced catalytic ability than the non-covalent co-assemblies (CA-YH). Remarkably, CCA-YH exhibited superior long-term stability than CA-YH and natural HRP under different pH, temperature, ion strength and organic medium environments, as well as a favorable reusability. Finally, CCA-YH was applied to the colorimetric detection of uric acid and sarcosine in human urine, which displayed satisfactory sensitivity, specificity and reproducibility. This is the first report the construction of stable peptide-based enzyme mimic based on covalent co-assembly strategy.
研究领域:
生物化学
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分析化学
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分子生物学
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DOI
高影响因子
ADVANCED POWDER TECHNOLOGY
·
2024
·
IF 4.2
Han Hao,Tiantian Ren,Cuilu Quan,Wei Guo,Jing Wang
Pharmaceutical cocrystal is an emerging strategy not only to improve physicochemical properties, but also to generate synergistic effects. In this work, a novel cocrystal composed by 5-fluorouracil (5-FU) and sarcosine (SAR) was successfully assembled by wet powder grinding method. The cocrystal was characterized by single crystal X-ray diffraction (SCXRD), powder X-ray diffraction (PXRD), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR) and Raman spectra. The differences of 1 H NMR spectroscopy between 5-FU/SAR physical mixture (PM) and cocrystal hinted the existence of supramolecular interactions between 5-FU and SAR in solid and solution. Bioavailability of cocrystal was increased than 5-FU. Most importantly, the weak interactions between 5-FU and SAR in cocrystal solution induced the superior cellar inhibition activity on 4T1 and BEL-7402 cells compared than 5-FU and PM. Cocrystal can interfere with the stability of methionine cycle and tetrahydrofolate (FH 4 ) level through the SAR-Gly-Met pathway, enhance the inhibition of thymidylate synthase (TS) compared to 5-FU, thereby interfering with cell cycle and inducing cell apoptosis. The results provided the novel strategy to develop the cocrystal drugs using bioactive amino acids as precursors to enhance the efficacy.
研究领域:
药学
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肿瘤学
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生物化学
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DOI
高影响因子
危险品化学品经营许可证（带存储）